• Journal of Species Research
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• v.13 no.1
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• pp.10-31
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• 2024

In this study, 11 strains of epilithic cyanobacteria belonging to six unrecorded species in Korea were isolated from gravel submerged in freshwater of seven collection sites in Korea. The morphological characteristics of the six species isolated in this study were consistent with the type strain of each species, and the similarity of the 16S rRNA gene sequences with the type strain of each species were 98.8-100%. In the phylogenetic tree using the 16S rRNA gene sequences, the 11 strains of these six species formed the same cluster as the strains of each species. The habitat of each previously reported species is mainly the soil surface, but all Korean strains appeared from the gravel submerged in freshwater. As a result of the morphological, ecological, and molecular analyses, these six species of cyanobacteria were identified as Geminocystis papuanica, Allocoleopsis franciscana, Ancylothrix terrestris, Klisinema persicum, Scytolyngbya timoleontis, and Shackletoniella antarctica, which were added as newly recorded species in Korea.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.99-107
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• 1996

Eighteen nuclear probes were used to examine RFLP(restriction fragment length polymorphism) between six species of Artemisia spp. of Korea. Total DNA from six different species of Artemisia was separately cut with three restrict enzymes. The PstI enzyme was showed to reduce the variation of polymorphisms than the other two enzymes(EcoRl and BamHI). The genetic variation of polymorphism was similar between the Dhewegiki-ssug and Cham-ssug. RAPD analysis was applied to the same six species of Artemisia spp. in order to assess the degree of DNA polymorphism within the Artemisia genus. Six species of Artemisia were evaluated for variation using a set of 11 random 10-mer primers. Nine out of the eleven primers revealed scorable polymorphisms between six species of Artemisia spp. Genetic distances between each of the species were calculated and cluster analysis was used to generate a dendrogram showing phylogenetic relationships between them This result indicates that molecular markers will be more usable in intraspecific study of Artemisia spp. than isoenzyme markers.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.2
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• pp.195-206
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• 2017

Joint time-frequency images of the broadband echo signals of six fish species were obtained using the smoothed pseudo-Wigner-Ville distribution in controlled environments. Affine transformation and principal component analysis were used to obtain eigenimages that provided species-specific acoustic features for each of the six fish species. The echo images of an unknown fish species, acquired in real time and in a fully automated fashion, were identified by finding the smallest Euclidean or Mahalanobis distance between each combination of weight matrices of the test image of the fish species to be identified and of the eigenimage classes of each of six fish species in the training set. The experimental results showed that the Mahalanobis classifier performed better than the Euclidean classifier in identifying both single- and mixed-species groups of all species assessed.

• The Korean Journal of Mycology
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• v.51 no.1
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• pp.7-24
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• 2023

More than five hundreds of yeast species (including 9 variants) encompassing 142 genera and 48 classes of 2 phyla exist in Korea. However, only 173 species have been cataloged in the National Species List of Korea (NSLK), the backbone reference to claim sovereign rights over biological resources, as of December 2021, due to the lack of taxonomic descriptions, although some of these species are extensively used in industry. The present pilot study investigated the taxonomy of strains belonging to the six most widely used or frequently isolated yeast species (Meyeromyma guilliermondii, Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Wickerhamomyces anomalus, Candida tropicalis, and Papiliotrema flavescens) to include these species in the NSLK. Strains with diverse habitats and geographic origins were retrieved from the National Institute of Biological Resources culture collection. These strains clustered in the same clade as the type strains of the designated species according to phylogenetic analysis of the D1/D2 sequences. Moreover, we described the cell morphology and physiological characteristics of representative strains of each species. This study suggests that these six species are indigenous to Korea and can be accordingly listed in the NSLK.

• Animal Systematics, Evolution and Diversity
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• v.30 no.3
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• pp.196-205
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• 2014

This paper describes six new species of sponges in the genus Agelas from Kosrae Island, The Federated States of Micronesia. Most Agelasid sponges are known from only tropical regions. All the new Agelas species; A. fragum n. sp., A. kosrae n. sp., A. purpurea n. sp., A. bakusi n. sp., A. vansoesti n. sp. and A. incrustans n. sp. are compared with other valid species that were studied. Six new species differ from the other species by morphology, growth form, skeletal fibres, habitats and spicule size. Agelas fragum n. sp. is characterized by its tuberculate surface and primary fibres with brush-like spicules. Agelas kosrae n. sp. is differs in skeletal structure and have tertiary fibres. Agelas purpurea n. sp. is characterized by primary, secondary and tertiary fibres are all cored with spicules. Agelas bakusi n. sp. is similar to Agelas clathrodes in shape, but differs in the primary fibres. Agelas vansoesti n. sp. is characterized by having acanthostrongyles. Agelas incrustans n. sp. is distinguished by its encrusting and not cavernous interior.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.461-470
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• 2023

The indigenous fungi in Mudeungsan National Park were investigated between 2020 and 2022. All collected specimens were identified to the species level based on their morphological characteristics and rDNA sequences. Species belonging to the family Boletaceae were analyzed in depth. Six species, Aureoboletus sinobadius, Hourangia densisquamata, H. nigropunctata, Tengioboletus glutinosus, Tylopilus himalayanus, and Xerocomus subparvus were newly recorded as macromycota in Korea.

• Journal of Microbiology
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• v.41 no.4
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• pp.312-319
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• 2003

In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

• Development and Reproduction
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• v.23 no.4
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• pp.367-375
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• 2019

Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

• Animal Systematics, Evolution and Diversity
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• v.25 no.1
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• pp.49-59
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• 2009

One new species and six unrecorded are described with illustrations based on the specimens collected in 1970s-1990s in Korea. The genera Phaenopsectra, Xenochironomus, and Smittsia are reported for the first time in Korea.

• Animal Systematics, Evolution and Diversity
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• v.35 no.4
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• pp.182-185
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• 2019

In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.