• Title/Summary/Keyword: Site-specific mapping

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Climatic Suitability Mapping of Whole-Crop Rye Cultivation in the Republic of Korea

  • Peng, Jing Lun;Kim, Kyung Dae;Jo, Mu Hwan;Kim, Moon Ju;Lee, Bae Hun;Kim, Ji Yung;Chemere, Befekadu;Kim, Si Chul;Kim, Byong Wan;Sung, Kyung Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.337-342
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    • 2018
  • This study was conducted to perform the suitability analysis of whole-crop rye (Secale cereale L.) based on the climatic information in the Republic of Korea to present useful information for producers and policy makers to determine the site-selection for the cultivation of the whole-crop rye. The criteria to analyze the climatic suitability of whole-crop rye was developed firstly. Then, the climatic suitability map for spatial analysis was developed through weighted overlaying the raster layers of climatic items in the evaluation criteria. Meanwhile, 16 geographically representative weather stations were selected to show examples of the calculation process of the climatic suitability score of a specific cultivation area. The results of the climatic suitability mapping indicated that the climatic conditions in most arable lands of the Republic of Korea such as the coastal, southern, western areas in the southern region of the Korean Peninsula and central areas in Jeju Island are suitable for the cultivation of whole-crop rye. The climatic suitability scores of the 16 weather stations were all in line with the results of the climatic suitability map.

Object-Oriented Field Information Management Program Developed for Precision Agriculture

  • Sung J. H.;Choi K. M.
    • Agricultural and Biosystems Engineering
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    • v.4 no.2
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    • pp.50-57
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    • 2003
  • This study was conducted to develop software which provides automatic site-specific field data acquisition, data processing, data mapping and management for precision agriculture. The developed software supports acquisition and processing of both digital and analog data streams. The architecture was object-oriented and each component in the architecture was developed as a separate class. In precision agriculture research, the laborious task of manual ground-truth data collection will be avoided using the developed software. In addition, gathering high-density data eliminates the need for interpolation of values for un-sampled areas. This software shows good potential for expansion and compatibility for variable-rate-application (VRA). The FIM (Field Information Management) computer program provides the user with an easy-to-follow process for field information management for precision agriculture.

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Identification of promoter sites in Babesia equi ema-l 5' intergenic nucleotide: I. PCR amplification and restriction mapping (Babesia equi ema-l 5' intergenic 뉴클레오타이드의 프로모터 위치 확인: I. PCR 증폭 및 제한효소지도)

  • 곽동미
    • Korean Journal of Veterinary Service
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    • v.27 no.1
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    • pp.103-109
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    • 2004
  • Babesia equi ema-1 5' intergenic(IG) nucleotide was PCR amplified and analyzed for restriction sites in order to identify a promoter region in this IG nucleotide sequence. B equi ema-1 5' IG specific primers identified a 1268 bp PCR product. The sequence had restriction sites for 34 restriction enzymes when analyzed by a computer program. Among them, 26 enzymes had only one restriction site, but the others had more than one sites. When four restriction enzymes (Bgll , HindⅢ, Kpn1 and BamH1) were treated to digest the 1268 bp nucleotide, they had restriction sites as expected by the computer program. Information of restriction sites in the 1268 bp IG nucleotide will be applied to select restriction enzymes for cloning the IG nucleotide to a vector.

2-dimensional Mapping of Sulfur Dioxide and Bromine Oxide at the Sakurajima Volcano with a Ground Based Scanning Imaging Spectrograph System

  • Lee, Han-Lim;Kim, J.-Hoon;Ryu, Jae-Yong;Kwon, Soon-Chul;Noh, Young-Min;Gu, Myo-Jeong
    • Journal of the Optical Society of Korea
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    • v.14 no.3
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    • pp.204-208
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    • 2010
  • A scanning imaging spectrograph system was used in this study to retrieve readings of the 2-D distribution of $SO_2$ and BrO around the crater of the Sakurajima volcano in Japan. The measurement was carried out during the daytime on November 2, 2005. Measurements were made at the surface of the site, located 5 km from the Sakurajima crater. One hundred horizontal scans were performed. Each column scanned by the system consists of 64 vertical pixels in order to retrieve the spatial distributions of BrO and $SO_2$ in the plume in terms of slant column densities (SCDs). Measured spectra were analyzed to identify and quantify $SO_2$ and BrO in the volcanic plume utilizing the plume's specific absorption features in the ultra violet region. Two-dimensional BrO and $SO_2$ distributions in SCD were retrieved horizontally covering the upwind, crater and downwind areas, and vertically, including the plume in the center of the scanned image. Both horizontal and vertical dispersions of $SO_2$ SCD from the crater were successfully measured to be from $10^{17}$ to $4.5{\times}10^{18}$ molecules $cm^{-2}$. However, BrO was measured below $10^{15}$ molecules $cm^{-2}$, which is considered its background level.

Mapping Categories of Heterogeneous Sources Using Text Analytics (텍스트 분석을 통한 이종 매체 카테고리 다중 매핑 방법론)

  • Kim, Dasom;Kim, Namgyu
    • Journal of Intelligence and Information Systems
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    • v.22 no.4
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    • pp.193-215
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    • 2016
  • In recent years, the proliferation of diverse social networking services has led users to use many mediums simultaneously depending on their individual purpose and taste. Besides, while collecting information about particular themes, they usually employ various mediums such as social networking services, Internet news, and blogs. However, in terms of management, each document circulated through diverse mediums is placed in different categories on the basis of each source's policy and standards, hindering any attempt to conduct research on a specific category across different kinds of sources. For example, documents containing content on "Application for a foreign travel" can be classified into "Information Technology," "Travel," or "Life and Culture" according to the peculiar standard of each source. Likewise, with different viewpoints of definition and levels of specification for each source, similar categories can be named and structured differently in accordance with each source. To overcome these limitations, this study proposes a plan for conducting category mapping between different sources with various mediums while maintaining the existing category system of the medium as it is. Specifically, by re-classifying individual documents from the viewpoint of diverse sources and storing the result of such a classification as extra attributes, this study proposes a logical layer by which users can search for a specific document from multiple heterogeneous sources with different category names as if they belong to the same source. Besides, by collecting 6,000 articles of news from two Internet news portals, experiments were conducted to compare accuracy among sources, supervised learning and semi-supervised learning, and homogeneous and heterogeneous learning data. It is particularly interesting that in some categories, classifying accuracy of semi-supervised learning using heterogeneous learning data proved to be higher than that of supervised learning and semi-supervised learning, which used homogeneous learning data. This study has the following significances. First, it proposes a logical plan for establishing a system to integrate and manage all the heterogeneous mediums in different classifying systems while maintaining the existing physical classifying system as it is. This study's results particularly exhibit very different classifying accuracies in accordance with the heterogeneity of learning data; this is expected to spur further studies for enhancing the performance of the proposed methodology through the analysis of characteristics by category. In addition, with an increasing demand for search, collection, and analysis of documents from diverse mediums, the scope of the Internet search is not restricted to one medium. However, since each medium has a different categorical structure and name, it is actually very difficult to search for a specific category insofar as encompassing heterogeneous mediums. The proposed methodology is also significant for presenting a plan that enquires into all the documents regarding the standards of the relevant sites' categorical classification when the users select the desired site, while maintaining the existing site's characteristics and structure as it is. This study's proposed methodology needs to be further complemented in the following aspects. First, though only an indirect comparison and evaluation was made on the performance of this proposed methodology, future studies would need to conduct more direct tests on its accuracy. That is, after re-classifying documents of the object source on the basis of the categorical system of the existing source, the extent to which the classification was accurate needs to be verified through evaluation by actual users. In addition, the accuracy in classification needs to be increased by making the methodology more sophisticated. Furthermore, an understanding is required that the characteristics of some categories that showed a rather higher classifying accuracy of heterogeneous semi-supervised learning than that of supervised learning might assist in obtaining heterogeneous documents from diverse mediums and seeking plans that enhance the accuracy of document classification through its usage.

Re-Engineering of Carcinoembryonic Antigen RNA with the Group I Intron of Tetrahymena thermophila by Targeted Trans-Splicing

  • JUNG HEUNG-SU;LEE SEONG-WOOK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.6
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    • pp.1408-1413
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    • 2005
  • Elevated expression of carcinoembryonic antigen (CEA) has been implicated in various biological aspects of neoplasia such as tumor cell adhesion, metastasis, blocking of cellular immune mechanisms, and antiapoptosis function. Thus, the CEA could be an important target for anticancer therapy. In this study, we developed Tetrahymena group 1 intron-based trans-splicing ribozymes that can specifically target and replace CEA RNA. To this end, we first determined which regions of the CEA RNA were accessible to ribozymes by employing an RNA mapping strategy that was based on a trans-splicing ribozyme library. Next, we assessed the ribozyme activities by comparing the trans-splicing activities of several ribozymes that targeted different regions of the CEA RNA, and then the ribozyme that could target the most accessible site was observed to be the most active with high fidelity in vitro. Moreover, the specific trans-splicing ribozyme was found to react with and altered the target CEA transcripts in mammalian cells with high fidelity. These results suggest that the Tetrahymena ribozyme can be utilized to replace CEA RNAs in tumors with a new RNA-harboring anticancer activity, thereby hopefully reverting the malignant phenotype.

Molecular cloning and restriction endonuclease mapping of homoserine dehydrogenase gene (HOM6) in yeast saccharomyces cerevisiae (Aspartate계 아미노산 대사 효모 유전자 HOM6의 cloning 및 구조분석)

  • 김응기;이호주
    • Korean Journal of Microbiology
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    • v.24 no.4
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    • pp.357-363
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    • 1986
  • Synthesis of threonine and methionine in yeast, Saccharomyces cerevisiae shares a common pathway from aspartate via homoserine. HOM6 gene encodes homoserine dehydrogenase (HSDH) which catalyzes the inter-conversion of beta-aspartate semialdehyde and homoserine. The level of HSDH is under methionine specific control. A recombinant plasmid (pEK1: 13.3kb), containing HOM6 gene, has been isolated and cloned into E. coli by complenemtary transformation of a homoserine auxotrophic yeast strain M-20-20D (hom6, trp1, ura3) to a prototrophic M20-20D/pEK1, using a library of yeast genomic DNA fragments in a yeast centromeric plasmid, YCp50(8.0kb). Isolation of HOM6has been primarily confirmed by retransformation of the original yeast strain M20-20D, using the recombinant plasmid DNA which was extracted from M20-20D/pEK1 and subsequently amplified in E. coli. Eleven cleavage sites in the insery (5.3kb) have been localized through fragment analysis for 8 restriction endonucleases; Bgl II(2 site), Bgl II(1), Cla I(3), Eco RI(1), Hind III(2), Kpn I (1), Pvu II(1) and Xho I(1).

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A Molecular Marker Specific to Metarhizium anisopliae var. majus

  • YOON, CHEOL-SIK;GI HO SUNG;JAE MO SUNG;JAEANG OON LEE
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.334-339
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    • 1999
  • More innovative molecular markers were investigated for rapid and consistent differentiation of Metarhizium anisopliae var. majus from M. anisopliae var. anisopliae. A total of 28 isolates were obtained from various countries and hosts: 13 isolates of M. anisopliae var. anisopliae, 12 isolates of M. anisopliae var. majus, and 3 isolates of M. anisopliae collected in Korea. This study involved restriction enzyme digestions of a PCR product amplified from nuclear internally transcribed spacer (ITS) and a portion of the 28S rDNA regions. Among 11 different restriction enzymes used in this study, MboⅠ digestion particularly produced a restriction pattern that had characteristics of M. anisopliae var. majus. This restriction pattern was consistent in all isolates of M. anisopliae var. majus regardless of their geographic origins and insect hosts. Mapping experiments revealed that MboⅠ sites of M. anisopliae var. majus are identical to those of M. anisopliae var. anisopliae with an exception for the presence of an additional site in the PCR product. Results from this study provide an additional method for identification and differentiation of isolates of these two varieties of M. anisopliae for use in the field and laboratory experiments.

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Sequence Analysis, Molecular Cloning and Restriction Mapping of Mitochondreal Genome of Domesticated Silkworm, Bombyx mori (누에 미토콘드리아 유전체의 제한효소 지도작성, 클로닝 및 염기서열 분석)

  • 이진성;성승현;김용성;서동상
    • Journal of Sericultural and Entomological Science
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    • v.42 no.1
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    • pp.14-23
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    • 2000
  • The mitochondrial genome of domesticated silkworm (Bombyx mori) was mapped with five restriction endonucleases (BamHI, EcoRI, HindIII, PstI and XbaI), the entire genome was cloned with HindIII and EcoRI. From the end sequencing results of 5$^1$and 3$^1$region for full genome set of eleven mitochondrial clones, the seven mitochondrial genes (NADH dehydrogenase 6, ATPase 6, ATPase 8, tRN $A^{Lys}$, tRN $A^{Asp}$, tRN $A^{Thr}$ and tRN $A^{Phe}$ of mori were identified on the basis of their nucleotide sequence homology. The nucleotide composition of NADH dehydrogenase 6 was heavily biased towards adenine and thymine, which accounted for 87.76%. On basis of the sequence similarity with published tRNA genes from six insect species, the tRN $A^{Lys}$, tRN $A^{Asp}$ and tRN $A^{Thr}$ were showed stable canonical clover-leaf tRNA structures with acceptible anticodons. However, both the DHU and T$\psi$C arms of tRN $A^{Phe}$ could not form any stable stem-loop structure. The two overlapping gene pairs (tRN $A^{Lys}$ -tRN $A^{ASP}$ and ATPase8-ATPase6) were found from our sequencing results. The genes are encoded on the same strad. ATPase8 and ATPase6 overlaps (ATGATAA) which are a single example of overlapping events between abutted protein-coding genes are common, and there is evidence that the two proteins are transcribed from a single bicistronic message by initiation at 5$^1$terminal start site for ATPase8 and at an internal start site for ATPase6. Ultimately, this result will provide assistance in designing oligo-nucleotides for PCR amplification, and sequencing the specific mitochondrial genes for phylogenetics of geographic races, genetically improved silkworm strains and wild silkworm (mandarina) which is estimated as ancestal of domesticated silkworm.sticated silkworm.

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Multi-Buffer Zone Analysis of Geo-Based Integrated Thematic Mappable Information by Using GIS (GIS를 이용한 지질자료 기반 통합 주제정보의 다중 버퍼 영역분석)

  • 이기원;박노욱;권병두
    • Spatial Information Research
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    • v.7 no.2
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    • pp.159-173
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    • 1999
  • GIS has been regarded as one of important tools or methodologies for various geoscience applications. Recently, spatial data integration schemes for site-specific or field-specific thematic mapping are newly developed and utilized. However, these kinds of approaches are somewhat insufficient quantitative assessment of integrated layers towards known targets in-detailed . Moreover, GIS analysis scheme is rarely extended to scientific approaches. In this study, simple approach of Multi-Buffer Zone Analysis , related to GIS analystical aspect, is addressed and an actual application for predicting or favorable mapping of mineral occurrences, one of GIS-based geoscientific approaches, is performed, As for geo-processing in GIS itself, this scheme can be regarded as extension or adaptiation of cell-based buffering or proximity analysis to geoscientific data interpretation. This study is based on rationale that surface geological pattern around primitives such as a point, a line, or a polygon in GIS, representing significant geological features, can be efficiently utilized to delineate complex geological behaviors or events, especially handling multiple dta sets originated from multiple sources such as airborne geophysical/radiometric exploration, field survey, and even a classified image of remote sensing. Conclusively, this methodology associated wit GIS is though to be helpful to analyze the spatial pattern of multiple data, pointing given sources, and is expected to effectively utilize for exploratory analysis of cell-based resultant layer integrated with complex or different data sources.

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