• 멤브레인
• /
• 제25권4호
• /
• pp.301-309
• /
• 2015

최근 해수담수화 기술에 관한 관심이 증가하면서 해수 전처리 기술의 중요성이 날로 증가하고 있다. 해수담수화 기술은 미래 수처리 핵심기술로 자리매김하고 있고 이를 위한 전처리 기술의 올바른 선택과 운영은 향후 해수담수화 기술의 효율향상과 공정 최적화를 위해 중요하게 고려되어야 할 것이다. 해수담수화 전처리 기술의 목적은 주로 해수에 존재하는 입자성 물질, 콜로이드성 물질, 유기물질, 무기물질 그리고 미생물 오염물질 등의 처리를 통해 후단 담수화 기술의 효율성을 향상시키기 위함이나 전처리 기술 대상 처리물질의 범위는 매우 다양하여 맞춤형 전처리 기술의 적절한 적용이 필요하다. 해수담수화에서 올바른 전처리 기술의 적용은 후단 담수화 시설의 높은 처리효율 및 문제점을 최소화시킴과 동시에 해수의 큰 수질변동과 기후적인 그리고 지역적인 영향 등에 즉각적으로 대처할 수 있으므로 전처리 기술의 운영전략은 미래 해수담수화 기술의 성공여부를 결정짓기 위해 매우 중요하게 다루어져야 한다. 또한 최근에 많은 관심을 가지고 있는 해수 미세조류의 번성은 담수화 전처리 기술의 선정에 있어서 잠재적인 장애가 되고 있어 이에 대한 올바른 이해도 반드시 필요하다. 본 총설에서는 해수담수화 전처리 기술에 관한 그동안의 연구동향을 분석하고 해수담수화 전처리 기술의 선택 및 운전 최적화 달성을 위한 향후 도전과제들을 제시하고자 한다.

• 대한치과마취과학회지
• /
• 제14권3호
• /
• pp.157-165
• /
• 2014

Background: Incisional site of surgical operation become transient ischemic state and then occur reoxygenation due to vasodilatation by inflammatory reaction, the productive reactive oxygen species (ROS) give rise to many physiologic results. Apoptosis have major role on elimination of inflammatory cell and formation of granulation tissue in normal wound healing process. Remifentanil can prevent the inflammatory response and can suppress inducible nitric oxide synthase expression in a septic mouse model. After cardiopulmonary bypass for coronary artery surgery, remifentanil can also inhibit the release of biomarkers of myocardial damage. Here we investigated whether remifentanil pretreatment has cellular protective effect against hypoxia-reoxygenation in HaCaT human keratinocytes, if so, the role of apoptosis and autophagy on this phenomenon. Methods: The HaCaT human keratinocytes were exposed to various concentrations of remifentanil (0.01, 0.05, 0.1, 0.5 and 1 ng/ml) for 2 h before hypoxia (RPC/HR group). These cells were cultured under 1% oxygen tension for 24h at $37^{\circ}C$. After hypoxia, to simulate reoxygenation and recovery, the cells were reoxygenated for 12 h at $37^{\circ}C$. 3-MA/RPC/HR group was treated 3-methyladenine (3-MA), autophagy inhibitor for 1h before remifentanil treatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazoliumbromide (MTT, amresco), showing the mitochondrial activity of living cells. To investigate whether the occurrence of autophagy and apoptosis, we used fluorescence microscopy and Western blot analysis. Results: The viability against hypoxia-reoxygenation injury in remifentanil preconditioning keratinocytes were increased, and these cells were showed stimulated expression of autophagy 3-MA suppressed the induction of autophagy effectively and the protective effects on apoptosis. Atg5, Beclin-1, LC3-II and p62 were elevated in RPC/HR group. But they were decreased when autophagy was suppressed by 3-MA. Conclusions: Remifentanil preconditioning showed the protective effect in human keratinocytes, and we concluded that autophagy may take the major role in the recovery of wound from hypoxia-reoxygenation injury. We suggest that further research is needed about the cell protective effects of autophagy.

• Journal of Periodontal and Implant Science
• /
• 제26권4호
• /
• pp.889-905
• /
• 1996

It is known that growth factors function as potent biologic mediators regulating numerous activities of wound healing via cell proliferation, migration and extracellular matrix formation and they also promote periodontal regeneration. But, method of growth factor application is controversial yet. So purpose of this study is to evaluate the effect of demineralized root surface as one of method of growth factor application. The ginigival fibroblasts were primary cultured and fifth or sixth subpassages were used in these experiments. In first experiment, root surface blocks demineralized with 100mg/ml tetracycline for 5 minutes and pH 1 citric acid for 3 minutes(experimental groups) and nonteminerilized root surface blocks (control groups) were placed in 100ng/ml PDGF-BB for 5 minutes. Then the cells were seeded on each root surface blocks and cultured for 6, 24, 48, 72 hours. In second experiment, root surface blocks deminerilized with tetracycline and citric acid and nondemineralized root surface blocks were placed in 200ng/ml PDGF-BB for 5 minutes and another non-demineralized root surfcae blocks were placed in DMEM without PDGF-BB. At 1, 2, 4, 6, 8 days, the cells were seeded in 24-well plate and using of each eluent, cultured for 72 hours. The results of the four determinants were presented as mean and S.D.. The results were as follows : The attachment and proliferation of human gingival fibroblast on root surface were more increased when PDGF-BB was applicated on root surfrace demineralized with tetracycline or citric acid than non-demineralized root surface. And, in comparision tetracycline with citric acid, there were more attachment and proliferation of human gingival fibroblast on root surface demineralized with tetracycline than citric acid, and proliferation of human gingival fibroblast on demineralized root surface was increased time dependently 1 day to 3 days. In second experiment using eluent, proliferation of human gingival fibroblast was more increased to 6 days when human gingival fibroblast was cultured in eluent that PDGF-BB was applicated on demineralized root surface than two control groups, and degree of proliferation was decreased time dependently 1 day to 6 days. Proliferation of human gingival fibroblast cultured in eluent without PDGF-BB was constant 1 day to 6 days. After 6 days, degree of proliferation of human gingival fibroblast was similar in four groups. This means that release duration of PDGF-BB from demineralized root surface is 6 days. And in comparision tetracycline with citric acid, there was more proliferation of human gingival fibroblast in tetracycline-treated group than citric acid. In conclusion, demineralized root surface as primary site for PDGF-BB application, especially demineralized with tetracycline has important roles in attachment and proliferation of human gingival fibroblast, and may be useful clinical applications in periodontal regenerative procedures.

• Journal of Periodontal and Implant Science
• /
• 제49권6호
• /
• pp.355-365
• /
• 2019

Purpose: The modified minimally invasive surgical technique (M-MIST) has been successfully employed to achieve periodontal regeneration. Platelet-rich fibrin (PRF) is known to enhance wound healing through the release of growth factors. This study aimed to observe the outcomes of periodontal surgery when M-MIST was used with or without PRF for the treatment of isolated intrabony defects. Methods: This randomized clinical trial was conducted on 36 systemically healthy patients, who had chronic periodontitis associated with a single-site buccal probing pocket depth (PPD) and clinical attachment level of ≥5 mm. Patients were randomly divided into 2 groups: the test group treated with M-MIST and PRF, and the control group treated with M-MIST alone. The primary periodontal parameters analyzed were PPD, relative attachment level (RAL), and relative gingival margin level. The radiographic parameters analyzed were change in alveolar crest position (C-ACP), linear bone growth (LBG), and percentage bone fill (%BF). Patients were followed up to 6 months post-surgery. Results: Intragroup comparisons at 3 and 6 months showed consistently significant improvements in PPD and RAL in both the groups. In intergroup comparisons, the improvement in PPD reduction, gain in RAL, and the level of the gingival margin was similar in both groups at 3 and 6 months of follow-up. Furthermore, an intergroup comparison of radiographic parameters also demonstrated similar improvements in C-ACP, LBG, and %BF at 6 months of follow-up. Conclusions: M-MIST with or without PRF yielded comparable periodontal tissue healing in terms of improvements in periodontal and radiographic parameters. Further investigation is required to confirm the beneficial effects of PRF with M-MIST.

• Journal of Radiation Protection and Research
• /
• 제24권2호
• /
• pp.109-120
• /
• 1999

TRIGA Mark-II, III 연구로를 폐로함에 따라, 원자로를 비롯한 관련 시설로부터 작업자의 방사선피폭을 최소화하고 환경으로의 방사성물질 누출을 사전에 방지하며, 안전한 해체방법을 모색하여 해체공사시의 기술기준을 수립하기 위하여 해체대상 시설내에 잔존하고 있는 방사성물질의 방사선/능 준위를 측정 및 분석 평가하였다. 측정대상은 시설내의 바닥 및 벽면과 잔존 실험시설물의 표면, 수조내 방사화 물질의 표면, 시설내 잔존하고 있는 냉각수 등이다. 이들 측정대상에 대한 방사선량율과 표면오염도, 핵종 등을 측정, 분석 및 평가하였다. 또한 전산코드 Fispin 을 사용하여 원자로 수조내의 주요 방사화 물질에 대한 방사능양과 핵종을 평가하였다. 본 평가 결과는 해체계획서 작성시 기본자료로서 유용하게 사용되었다.

• Journal of Radiation Protection and Research
• /
• 제28권4호
• /
• pp.311-319
• /
• 2003

본 연구는 격자형 가우시안 플룸모형을 Matlab언어를 이용하여 구축한 후, 영광원자력시설의 부지에서 시행된 추적자 확산실험자료를 이용하여 예측력을 평가하였다. 풍하방향으로는 20km까지 10m간격으로 격자를 구분하였으며, 풍하방향에 수직인 지표방향은 방출점을 중심으로 상하 5km를 각각 10m 간격으로 구분하여 $1,990{\times}1,000{\times}1$의 격자망으로 구성하였다. 실험당시의 대기안정도는 P-G방법에 의해 B등급으로 나타났으며 이를 이용하여 각 격자의 농도예측을 수행하였다. 반경 3km의 A-line의 경우가 반경 8km근방의 B-line에 비해 격자형 가우시안 모형의 예측력이 뛰어난 것으로 나타났으며, 방출점에서 거리가 멀어질수록 P-G방법에 의한 확산폭의 산정은 모형의 예측력을 떨어뜨리는 것으로 나타났다. 모형의 예측력을 향상시키기 위하여 P-G 방법에 의한 확산폭인 sigma y 및 sigma z를 선형계획법을 이용하여 수정하였다. 수정된 확산인자를 적용한 결과 3km와 8km 모두 모형의 예측력이 향상됨을 확인할 수 있었다. 향후 추적자 확산실험 데이터의 축적을 통해 기상조건에 따른 확산인자에 대한 경험식을 개발한다면 격자형 가우시안 모델이 원자력시설에서의 대기질 환경영향평가에 유용하게 쓰일 수 있을 것으로 기대된다.

• 한국수자원학회논문집
• /
• 제34권5호
• /
• pp.487-497
• /
• 2001

옥정호에 대한 수질변화 조사 자료를 토대로 수질모의를 실시하였으며, 그 결과 바탕으로 효율적인 댐저수지 수질관리 방안을 제시하였다. 옥정호의 수질은 계절에 다라 중영양상태와 부영양상태를 나타내는 것으로 평가되었으며 이러한 수질상태는 외부로부터 유입되는 오염원에 많은 영향을 받게 되므로 우선 유역으로부터 유입되는 오염부하의 정확한 산정이 필요한 것으로 평가되었다. 1999년에 조사한 7차례의 강우기간 중 총 부하와 일년간 발생한 총 부하를 비교하면 강우량은 17.5%인데 비해 SS는 72.8%, TN과 TP는 약 50%가 강우기에 유입되는 것으로 조상되었다. 또한 퇴적물의 용출율은 TP의 경우 가두리 양식장이 운영되던 지점에서는 11.92 mg/L, 정기수질 조사지점에서 0.2∼1.9 mg/L로 국내·외 자료와 비교하여 용출율이 작은 것으로 나타났다. 댐저수지 수질관리 방안 수립을 위하여 WASP5 모형을 적용하였으며 검증된 결과를 이용하여 각 소유역에서 오염부하 삭감에 따른 6가지 방안에 대한 수질모의를 실시하였고 각각에 대한 수질개선 효과를 제시하였다. 가장 효율적인 방안은 임실천 유역과 관촌교 유역의 오염부하를 제어하는 방안으로 평가되었으며, 가장 수질개선효과가 좋은 것은 대표 세유역의 오염부하를 제어하는 것으로 나타났다. 이를 바탕으로 수질개선을 위한 하·폐수처리장의 건설 적지를 제시하였다.

• 미생물학회지
• /
• 제50권3호
• /
• pp.239-244
• /
• 2014

ErmSF는 macrolide 항생물질인 tylosin을 생성하는 Streptomyces fradiae가 보유한 4개의 항생제 내성인자 단백질 중 하나로 23S rRNA의 $A_{2058}$에 dimethylation 시킴으로써 항생제가 부착되는 것을 막음으로써 그 내성을 일으킨다. ErmSF는 다른 Erm 단백질과는 달리 긴 N-terminal end region을 가지고 있어서 그 역할을 알아보기 위해 1-38번째의 아미노산을 제거한 결손변이 단백질을 고안하고 대장균에서 발현하여 그 활성을 in vivo와 in vitro에서 관찰하였다. 결손변이 단백질을 발현하는 대장균은 결손에 의한 활성저하에 기인하여 야생형 단백질을 발현하는 대장균에 비하여 항생제에 대한 내성이 손상된 것을 관찰하였다. 세포 외 in vitro에서의 활성은 야생형 ErmSF에 비하여 약 20%가 손상된 것으로 나타났다. 이렇게 관찰된 활성의 저하는 결손 변이에 의한 활성화 부위에서 일어난 결손에 의한 것이라기 보다는 기질의 부착 또는 생성물의 효소에서의 이탈 과정이 손상되어서 나타나는 것으로 사료된다.

• Journal of Animal Science and Technology
• /
• 제53권6호
• /
• pp.533-540
• /
• 2011

The objectives of experiments were to compare directly the effects of synchrony of slowly fermented (corn and corn gluten meal; C-CGM) and of rapidly fermented ingredients (barley and soybean meal; B-SBM) in the rumen on starch disappearances in gastrointestinal tracts (Experiment 1) and growth performance (Experiment 2) of Hanwoo steers in the feedlot barn. In experiment 1, four Hanwoo steers ($288{\pm}21$ kg) fitted with ruminal and "T" shaped duodenal cannula were placed in one pen with Calan gate and assigned randomly to a duplicate $2{\times}2$ Latin square design. In experiment 2, eight intact Hanwoo steers ($311{\pm}8$ kg) were assigned randomly to one of two pens with Calan gate to evaluate the effect of the same diets as like in experiment 1 on growth performance. There were no differences in ruminal pH, ammonia and total VFA concentrations between treatments. Percentage of apparent ruminal starch disappearance was 33.3% unit lower (p<0.05) for steers fed C-CGM than for steers fed B-SBM diets and this difference resulted in 268% higher (p<0.05) in duodenal starch flows for steers fed C-CGM diet than for steers fed B-SMB diet. There was significant increase (p<0.05) in quantity (927 vs. 400 g/d) of corn starch digested post-ruminally compared to barley starch. However, percentage of starch apparently digested post-ruminally was 8% higher (p=0.1) in steers fed fast synchrony diet with B-SBM than in steers fed slow synchrony diets with C-CGM. The differences of percentage and amount of starch apparently digested post-ruminally between C-CGM and B-SBM diets did not affect rice straw DM intake, average daily gain (ADG) and feed efficiency. In conclusion, there is some uncertainty in regards to the relationship between site of starch digestion and DM intake, ADG, and feed efficiency in this study.

• Archives of Plastic Surgery
• /
• 제36권4호
• /
• pp.432-436
• /
• 2009

Purpose: Negative pressure therapy has been used in various conditions to promote wound healing. It has also been used to secure a skin graft by improving microcirculation and improving tight adhesion between the graft and the recipient bed. To reduce post burn scar contracture and improve aesthetical result, many types of dermal substitutes have been invented and used widely. The goal of this study was evaluate usefulness of the VAC (Kinetic concepts Inc., San Antonio, TX) in improving the take rate and time to incorporation of Integra$^{(R)}$ in reconstruction of burn scar contracture. Methods: A retrospective study was performed from October, 2006 to December, 2008. The VAC was utilized for 11 patients. The average patient's age was 19.7 years (range 5 - 27) and average surface area was $785cm^2$ (range 24 - 1600). The burn scars were excised deep into normal subcutaneous tissue to achieve complete release of the scar, Integra$^{(R)}$ was sutured in place with skin staple와 Steri - strip$^{(R)}$. Then slit incisions were made on silicone sheet only with No.11 blade for effective drainage. The VAC was used as a bolster dressing over Integra$^{(R)}$. Negative - Pressure ranging from 100 to 125 mm Hg was applied to black polyurethane foam sponge trimmed to the appropriate wound size. An occlusive seal over the black polyurethane foam sponge was maintained by a combination of the occlusive dressing, OP - site$^{(R)}$. The VAC dressing changes were performed every 3 or 4 days until adequate incorporation was obtained. The neodermis appeared slightly yellow to orange color. When the Integra$^{(R)}$ deemed clinically incorporated, The VAC was removed and take was estimated with visual inspection. Very thin STSG(0.006 ~ 0.008 inches) was performed after silicone sheet removal. Result: The mean time for clinically assessed incorporation of Integra$^{(R)}$ was 10.00 days (range 9 - 12). The mean dressing change was 3.5 times until take was obtained. In All patients, Integra$^{(R)}$ had successful incorporation in tissue without serious complications. Conclusion: Integra$^{(R)}$ in combination with Vacuum - Assisted Closure(VAC) may be incorporated earlier than conventional dressing method.