• Journal of Acupuncture Research
• /
• v.19 no.4
• /
• pp.1-15
• /
• 2002

Objective : To observe the biological responses and therapeutic effects of moxibustion and review the trend of related study. Methods : We searched and investigated the journals supplied by Pubmed online site and Je-Han Oriental Medical Academy homepage with the key word "moxibustion". Results : 1. The biological responses of moxibustion reveal on skelectal, digestive, urinary systems, especially blood, angiological systems. 2. The therapeutic effect of moxibustion are analgesic action, controls of excitation or inhibitation of nerve system, improvement of blood circulation, nutrition in organs, increase of absorption of pathlogical product, controls of secreting glands, care of tuberculosis, increase of natural healing power etc. 3. Moxibustions effects on diarrhea, edema, diabetus mellitus, hyperlipiemia, tinnitus, osteoporosis, facial palsy, myopia, pimple etc. 4. Most of moxibustion studys related on immunofunctional actions and renal functional actions. 5. To elevated quality of studies, we needs well-designed epeerimental methods, efficient secure of experimental groups, appropriate statistical verification, accumulations of knowledges about data research. Conclusions : We find out moxibustion is remakble on clinincal therapeutic effects, from now on much more studies are needed to develop this therapy.

• Archives of Pharmacal Research
• /
• v.25 no.5
• /
• pp.643-646
• /
• 2002

The succinic semialdehyde dehydrogenase (SSADH) inhibitory component was isolated from the EtOAc fraction of Lactuca sativa through repeated column chromatography; then, it was identified as phytol, a diterpenoid, based on the interpretation of several spectral data. Incubation of SSADH with the phytol results in a time-dependent loss of enzymatic activity, suggesting that enzyme modification is irreversible. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of $6.15{\times}10^{-2}mM^{-1}min^{-1}.$ Complete protection from inactivation was afforded by the coenzyme $NAD^{+}$, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme; therefore, it seems likely that phytol covalently binds at or near the active site of the enzyme. It is postulated that the phytol is able to elevate the neurotransmitter GABA levels in central nervous system through its inhibitory action on one of the GABA degradative enzymes, SSADH.

• Molecules and Cells
• /
• v.20 no.2
• /
• pp.196-200
• /
• 2005

The neurofibromatosis type2 (NF2) tumor suppressor gene product, merlin, is structurally related to the ezrin-radixin-moesin (ERM) family of proteins that anchor the actin cytoskeleton to specific membrane proteins and participate in cell signaling. However, the basis of the tumor suppressing activity of merlin is not well understood. Previously, we identified a role of merlin as an inhibitor of the Ras-ERK signaling pathway. Recent studies have suggested that phosphorylation of merlin, as of other ERM proteins, may regulate its function. To determine whether phosphorylation of merlin affects its suppression of Ras-ERK signaling, we generated plasmids expressing full-length merlin with substitutions of serine 518, a potential phosphorylation site. A substitution that mimics constitutive phosphorylation (S518D) abrogated the ability of merlin to suppress effects of the Ras-ERK signaling pathway such as Ras-induced SRE transactivation, Elk-mediated SRE transactivation, Ras-induced ERK phosphorylation and Ras-induced focus formation. On the other hand, an S518A mutant, which mimics nonphosphorylated merlin, acted like wild type merlin. These observations show that mimicking merlin phosphorylation impairs not only growth suppression by merlin but also its inhibitory action on the Ras-ERK signaling pathway.

• Journal of the Korea Safety Management & Science
• /
• v.17 no.1
• /
• pp.45-52
• /
• 2015

The purpose of this investigation is to enhance the survival rate of patients by transporting them to the hospital within the golden hour through the operational improvement of emergency dispatch instruction. To this end, problems and improvements of current operating system were derived by carrying out a survey against paramedics of Incheon city in 2012 and analyzing the current emergency dispatch instruction. This study analyzed the emergency activity daily reports for one year from January 1 through December 31, 2012 and researched the consciousness of 119 emergency medical technician. According to the analysis of the survey, there were no meaningful differences in the on-site arrival times per triage. Therefore, the item of 'Emergency Classification' specified in the emergency dispatch instruction needs to be integrated in the scheme of "triage". Also, the feedbacks of the emergency action log and the emergency dispatch instruction are necessary for 'duty for operation' to review the adequacy to the severity after the end of emergency operation. Finally, the improvement of the system for the continuous communication between the paramedics and the command staff is necessary. This improvements as stated above are expected to contribute to raise survival rate of patients.

• Structural Monitoring and Maintenance
• /
• v.2 no.2
• /
• pp.95-113
• /
• 2015

The knowledge of the behavior of any roller compacted concrete (RCC) dam and its foundation is gained by studying the service action of the dam and its foundation using measurements of an external and internal nature. The information by which a continuing assurance of structural safety of the RCC dam can be gauged is of primary importance. Similarly, the fact that the information on structural and thermal behavior and the properties of concrete that may be used to give added criteria for use in the design of future RCC dams is of secondary importance. Wide spread attention is now being given to the installation of more expensive instrumentation for studying the behavior of concrete dams and reservoirs and forecasting of any adverse trends. In view of this, the paper traces installation and need of the comprehensive instrumentation scheme implemented to monitor the structural and thermal behavior of 102.4 m high RCC dam constructed near Mumbai in India. An attempt is made in the present paper to emphasize the need to undertake an instrumentation program and evaluate their performance during construction and post construction stage of RCC structures. Few typical results, regarding the thermal and structural behavior of the dam, obtained through instrumentation installed at the dam site are presented and compared with the design considerations. The fair agreement is seen in the response observed through instrumentation with that governing the design criteria.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2020.11a
• /
• pp.63-64
• /
• 2020

The purpose of this study is to find out how construction sites workers experience adaptation processes in relation to the prevention of safety accidents in the workplace through the method of grounded theory. In order to understand the experience of preventing safety accidents of workers at construction sites, a grounded theory analysis method was chosen. In order to achieve the purpose of this study, the first questionnaire was sent to 17 people working in the construction site by e-mail, and the participants of the study were met one by one for in-depth interviews. As a result of this study, the paradigm model of the experience of preventing safety accidents of workers at construction sites was classified into causal conditions, contextual conditions, central phenomena, intervening conditions, action/interactions, and outcomes. The conclusions of this study are as follows. First, forming a safety culture that can improve the safety awareness of construction sites is a priority. Second, it is necessary to improve self-management capabilities so that construction workers can accurately diagnose their current state such as their own body, emotion, and cognition and provide appropriate safety education. Third, providing safety education for construction workers with negative thoughts or distorted beliefs about safety accidents needs to include psychological treatment and counseling, such as methods of emotional purification, methods of relieving and managing stress, and methods of removing trauma.

• 제어로봇시스템학회:학술대회논문집
• /
• 2005.06a
• /
• pp.1158-1162
• /
• 2005

Drug delivery systems have been developed to reduce the side toxicity of drugs by localizing them in the site of action. But it depends on the circulation of the blood and it doesn't have the function of locomotive mechanism of itself for searching for the region of disease. However, this problem could be solved by nanobot which have the locomotive function. So, we mimic the movement of cell that can move in a human body. In this paper, to polymerize the encapsulated actin within the liposome, electroporation technique is employed. In order to optimize polymerization and depolymerization of the liposome, we compare the time of polymerization and depolymerization by concentration of crown ether. we synthesis the liposome which contain azobenzene Linked crown Ether conjugated Actin protein. Azobenze linked crown ether holds the K+ ion by exposure of UV light and this disturbs the actin polymerization. In result, UV light could control the liposome growth. Finally, we could develop the liposome robot and control the growth and degeneration of the liposome by external stimuli such s UV light. The merit of the controlling by UV light doesn't need to inject proteins which induce polymerization and depolymerization of actin protein.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1995.04a
• /
• pp.80-80
• /
• 1995

T4 Endonuclease V (Mw 16,000) acts as a repair enzyme for UV induced pyrimidine dimers in DNA. Many researchers have studied the biochemical characteristics of the enzyme. However the precise action mechanism of T4 endo V has not fully elucidated yet. In our laboratory NMR spectroscopy technique is being used for the structural study of T4 endo V. Because of its low temperature stability and high content of ${\alpha}$-helix, the conventional $^1$H NMR technique was inapplicable. Therefore we utilized stable isotope labeling technique and so far prepared about 10 amino acid specific labeled proteins. The HSQC spectra of amino acid specific labeled proteins will help us to interpret the triple resonance 3D, 4D data which are under processing, We also studied the behaviors of specific amino acid residues whose roles might be critical. When the enzyme labeled by $\^$15/N-Thr was mixed with the substrate oligonucleotide (semispecific -TT- sequence), one crosspeak in its HSQC spectrum was completely desappeared, which means that one of seven Thr residues is in the binding site of the enzyme with DNA, This result is well consistent with previous report that implicated the Thr 2 residue in the activity of the enzyme. Similar studies were carried on the behaviors of Arg and Tyr residues.

• Animal cells and systems
• /
• v.2 no.2
• /
• pp.223-227
• /
• 1998

We isolated two soluble forms of glutamate dehydrogenase isoproteins, GDH I and GDH II, from bovine brain. The regulation of GDH I and GDH II by phosphorylation and dephosphorylation has been examined in various conditions. There were dose- and time- dependent activation of the GDH isoproteins when phosphorylated by cAMP-dependent protein kinase. The phosphorylated GDH had 1.1 mol of covalently bound phosphate/mol of subunit and a 2-fold increased specific activity. The phosphorylated amino acid was identified as serine. When treated with alkaline phosphatase, the activities of the phosphorylated GDH isoproteins were reduced in dose and time dependent manner and returned to those of unphosphorylated enzymes. There were no significant differences between GDH I and GDH II in their sensitivities to the action of phosphorylation and dephosphorylation demonstrating that the microenvironmental structures of the phosphorylation site in GDH isoproteins are similar to each other, These results results suggest that the inter-conversion between less active form of brain GDH isoproteins and more active form is regulated by phosphorylation through cAMP-dependent protein kineses.

• Annals of Clinical Neurophysiology
• /
• v.3 no.2
• /
• pp.156-159
• /
• 2001

Miller Fisher syndrome(MFS) has been the focus of conflicting opinions regarding the peripheral versus the central nature of the site of major neural injury. We present our electrophysiological findings in one case of MFS to help clarify the pattern of peripheral nerve injury in this syndrome. A 45-year-old man visited our hospital due to sudden diplopia. Initial examination revealed internuclear opthalmoplegia. The next day, his symptoms rapidly aggravated to complete external ophthalmoplegia, ataxia, and areflexia with hand and foot numbness. Serial electrophysiological studies were performed. The results of brainstem evoked potential(BAEP) and blink reflex were normal in the serial studies. Motor and sensory nerve conduction study(NCS) were normal findings in second hospital day, but ulnar sensory nerve shows no sensory nerve action potential(SNAP) and sural sensory conduction velocity was delayed in 7th hospital day. Our patient's clinical presentation began to improve on 15th hospital day, and his electrophysiologic study showed improvement on 29th hospital day. We believe that all the manifestations of MFS can be explained by the involvement of peripheral nerves without brainstem or cerebellar lesion with the serial electrophysiological studies.