• Journal of Yeungnam Medical Science
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• v.15 no.1
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• pp.47-54
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• 1998

Interleukin-10(IL-10) inhibits production of a wide range of cytokines in various cell types and transcriptionally inhibits lipopolysaccharide (LPS)-induced expression of proinflammatory mediators. Cytokine expression by macrophages is an important aspect to ochestrate inflammatory responses. As an approach to identify mechanistic targets of IL-10, it was examined the time course for expression of KC(murine homologue of Gro) gene in murine peritoneal macrophages stimulated with LPS with or without IL-10. The effect of IL-10 on LPS induced KC mRNA expression was delayed and only seen after 1 hour treatment. Pretreatment with IL-10 did not eliminate the delayed inhibitory response nor increase the magnitude of suppression. These effects did not depend upon time of IL-10 treatment but the time of LPS treatment. LPS-induced KC mRNA expression by inhibitory action of IL-10 was not controlled at the level of transcription. The result indicates that IL-10 acts late in the process of KC gene expression and that the prominant site of action may be mRNA stability or translation.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1577-1583
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• 2014

Encapsulation is a method used to protect material from certain undesirable environments, for controlled release at a more favorable time and place. Animal productivity would be enhanced if feed additives are delivered to be utilized at their site of action, bypassing the rumen where they are likely to be degraded by microbial action. A novel method of encapsulation with sesame gum was used to coat nitrate, a known enteric methane mitigating agent, and tested for the effect on methane reduction and other in vitro fermentation parameters using rumen fluid from cannulated Hanwoo steers. Orchard grass was used as basal diet for fermentation. The treatments were matrix (1.1 g sesame gum+0.4 g sesame oil cake) only, encapsulated nitrate (matrix+nitrate [21 mM]), free nitrate (21 mM), and a control that contained no additive. Analyses of fermentation parameters were done at 0, 3, 6, 9, 12, 24, and 48 h time periods. In comparison to control, both free and encapsulated nitrate produced significantly reduced (p<0.01) methane (76% less) and also the total volatile fatty acids were reduced. A significantly higher (p<0.01) concentration of ammonia nitrogen was obtained with the encapsulated nitrate treatment (44%) compared to the free form (28%) and matrix only (20%) (p = 0.014). This might suggest slow release of encapsulated nitrate so that it is fully reduced to ammonia. Thus, this pioneering study found a significant reduction in methane production following the use of sesame gum encapsulated nitrate that shows the potential of a controlled release system in enhancing sustainability of ruminant production while reducing/eliminating the risk of nitrite toxicity.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.3
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• pp.597-610
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• 2014

The purpose of this study was to inquiry of influence of Meister high school and specialized vocational high school' students on career maturity in problem solving ability domain of test for enhanced employ ability & upgraded proficiency. Research subjects and region were sophomore students of each one of Meister high schools and specialized vocational high schools in Gwangju region. The sample(N=481) was drawn from 2 high schools and collected data were analyzed with SPSS using t-test, Pearson correlation and Levene's test. Items of problem solving ability domain of test for enhanced employ ability & upgraded proficiency were used test items developed in 2012. Career maturity test in Careernet site was used test items developed in 2001 which was made up 3 domains such as attitude, ability and action and 8 sub-regions such as planning, attitude on the vocation, independence, self-understanding, rational decision-making, explore of information, knowledge of hope vocation and career exploration and preparation of action, and constituted 64 items. The results were as follows. Findings of the analysis in specialized vocational high school students demonstrated the positive results of rational decision-making and explore of information in problem solving ability test for enhanced employ ability & upgraded proficiency. Comparing career maturity influence on Meister high school with specialized vocational high school students about problem solving ability domain of test for enhanced employ ability & upgraded proficiency, Meister high school students' score were more high grade than specialized vocational high school students grade.

• Korean Journal of Weed Science
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• v.14 no.1
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• pp.1-7
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• 1994

The effects of S-23142{N-(4-chloro-2-fluoro-5-propargyloxyphenyl)-3, 4, 5, 6-tetrahydrophtalimide}, on protoporphyrin IX(PPIX) biosynthesis in Spinacia oleracea L, leaf in vivo and in vitro condition were investigated by reversed-phase HPLC with fluorescence detector. The stroma and the membrane fraction of spinach chloroplast were isolated by osmotic regulation. The conversion of ${\delta}$-aminolevulinic acid(ALA) to PPIX occured more in the stroma than in the membrane fraction. It suggested that the enzymes that catalyse PPIX biosynthesis from ALA were localized in the stroma. Also, the synthesized PPIX content from ALA was completely inhibited by $10^{-8}M$ of S-23412 or $10^{-7}M$ of acifluorfen in the stroma but not in the membrane fractions. Therefore, these results suggested that the target site of S-23142 and acifluorfen may exist in the stroma fraction of spinach chloroplast.

• The Journal of Korean Medicine
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• v.22 no.2
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• pp.109-119
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• 2001

Objective : There are many reports that acupuncture has thermoregulatory effects on human and animals. To investigate the effect and mechanism of antipyretic action of acupuncture, we observed the body temperature and cytokine expressions in the hypothalamus of rats. Methods : Lipopolysaccharide (LPS, i.p., 2.5mg/kg) was injected to conscious rats (Sprague-Dawley, male, n=4l) to cause hyperthermia and simple needling (stainless steel, 0.25 mm o.d., 5 mm insertion for 10 sec with no manipulation) was performed bilaterally with the measurement of rectal temperature. Next, we sacrificed rats to remove brain and determined the level of mRNA for interleukin-6 (IL-6), $interleukin-1{\beta}{\;}(IL-1{\beta})$, interleukin-2 (IL-2) and $interferon-{\gamma}{\;}(IFN-{\gamma})$ in the hypothalamus by using reverse transcriptase-polymerase chain reaction (RT-PCR). Resul1s : Needling on forepaw (acupoint HT8) and needling on hindpaw (acupoint BL66 and acupoint LR2) significantly inhibited LPS-induced fever of rats (P<0.01, 10 min after treatment respectively), but same treatment on proximal tail (non-acupoint) did not cause any change on fever. The levels of IL-6 and $IL-1{\beta}$ mRNA in the hypothalamus was significantly enhanced by LPS-injection, while the level of IL-6 and $IL-1{\beta}$ mRNA was markedly reduced after treatment on BL66 (P<0.01). Treatment on forepaw reduced it slightly, but not significantly. Equivalent stimulation on proximal tail did not cause any changes. Conclusions : Our results indicate that acupuncture stimulation on various body parts has differential thermoregulatory effects on LPS-induced fever of rats with site-specificity. And, we suggest that its antipyretic action might be accompanied with the suppression of hypothalamic production of pro-inflammatory cytokine of immune system, IL-6 and $IL-1{\beta}$.

• World Technopolis Review
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• v.4 no.1
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• pp.23-32
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• 2015

Public-private partnerships (PPPs) are very popular governance practices, as they enable the private partner to engage in business and have profits while the public partner improves the provision of public services. PPPs are organizational arrangements with a sector-crossing or sector-blurring nature, and are modes of governance - governance by partnerships or collaborative governance (Schuppert 2011). New models and applications of PPPs have been developed over time. Collaborative governance entails information exchange, action or movement harmonization, resource sharing, and capacity enhancement among the partners (Sale 2011; 2012a). As the national university, the University of the Philippines (UP) serves as a research university in various fields of expertise and specialization by conducting basic and applied research and development, and promoting research in various colleges and universities, and contributing to the dissemination and application of knowledge, among other purposes. (Republic Act 9500) It is the site of two (2) science and technology parks (Sale 2012b), one of which is the UP - Ayala Land Technohub. A collaboration between industry and the academe, the Technohub is envisioned as an integrated community of science and technology companies building a dynamic learning and entrepreneurial laboratory (UP-AyalaLand Technohub). This paper takes a closer look at the UP - Ayala Land Technohub as an example of a PPP or collaborative governance in science and technology parks. Have information exchange, action or movement harmonization, resource sharing, and capacity enhancement taken place in the Technohub? What are some significant outcomes of, and issues arising from, the PPP? What assessment indicators may be used? Is a governance instrument needed?

• Biomolecules & Therapeutics
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• v.1 no.1
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• pp.1-8
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• 1993

It has been known that calcium antagonists also inhibit the radioligand binding to muscarinic and $\alpha$-adrenergic receptors and, in case of verapamil, these inhibitions may play a role in the effects of verapamil on the heart. In this study, the effects of nicardipine, nifedipine, nimodipine, diltiazem and verapamil on the binding of [$^3H$]dihydroalprenolol (DHA) to dog cardiac ${\beta}$-adrenergic receptors were examined. A single uniform [$^3H$]DHA binding site ($K_D/= 5nM\;and\;B_{max}=2600$ fmol/mg protein) was identified in dog cardiac sarcolemma. [$^3H$]DHA binding was not affected by the usual therapeutic concentrations of these calcium antagonists (nanomolar range) but in the "nonspecific"concentration ranges ($28-180{\mu}m$) these drugs inhibited [$^3H$]DHA binding to $\beta$-adrenergic receptors. Nicardipine, nifedipine, nimodipine and diltiazem competed for [$^3H$]DHA binding to ${\beta}$-adrenergic receptors with dissociation constants ($K_i$) of $28{\mu}m,\' 74{\mu}m, 39{\mu}m \;and \;35{\mu}m,$ respectively. Verapamil ($K_i=176.5 {\mu}m$) was less potent inhibitor than other drugs and this inhibition was noncompetitive; the maximal binding capacity ($B_{max}$) $300 {\mu}m$ verapamil without change in the apparent dissociation constant (4K_D$) for DHA. These results indicate that the inhibitory action of calcium antagonists at high concentrations on ${\beta}$-adrenergic receptors is not involved in the therapeutic effects of these drugs by the calcium channel blocking action.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.5
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• pp.64-71
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• 2018

This paper provides the results for experimental study on longitudinal shear performance for new-concept composite slabs embedded with silence insulator. Longitudinal shear capacity tests are used to predict characteristics of composite action between the concrete block, silence insulator and deckplate. A total set of 7 Push-Down specimens are tested to clarify the composite action between the concrete block, silence insulator and deckplate. Parameters in this study are the width of T-type bar, the depth of reinforced bar and the thickness of silence insulator. The results from experimental study on longitudinal shear capacity for new-concept composite slabs applied T-type bar, Reinforced bar and Silence insulator are summarized as follows. In test result, the Longitudinal Shear Capacity of the new concept specimen is 2 times excellent than basic specimen with safety. It is expected that applying the proposed composite slab detail at the actual site will provide a significant safety factor in structural aspect of the existing composite slab, and greatly contribute to the improvement capacities of resisting vibration and sound.

• Journal of Korean Library and Information Science Society
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• v.49 no.1
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• pp.99-122
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• 2018

The Korean government has established a plan on the 'service operating system of public libraries', which divides the local libraries into a central library, regional libraries, and small branches. However, due to the low interest of the libraries and, above all, the structural problems caused by the dualization of the library administrative bodies, no action plans have been developed. This study intends to propose an integrated operating system for public libraries in Busan metropolitan and to design specific roles and functions according to the library status. To the ends, this study examined the Korean government's plan for public library operation, analyzed the operation cases of foreign public libraries, and finally suggested the detailed action plan of an integrated operating system based on the case of Busan Metropolitan City. The data were collected from literature review and on site field survey, supplemented by e-mail inquiry and official statistical information.

• KIPS Transactions on Software and Data Engineering
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• v.10 no.5
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• pp.187-194
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• 2021

A person's behavioral recognition is the recognition of what a person does according to joint movements. To this end, we utilize computer vision tasks that are utilized in image processing. Human behavior recognition is a safety accident response service that combines deep learning and CCTV, and can be applied within the safety management site. Existing studies are relatively lacking in behavioral recognition studies through human joint keypoint extraction by utilizing deep learning. There were also problems that were difficult to manage workers continuously and systematically at safety management sites. In this paper, to address these problems, we propose a method to recognize risk behavior using only joint keypoints and joint motion information. AlphaPose, one of the pose estimation methods, was used to extract joint keypoints in the body part. The extracted joint keypoints were sequentially entered into the Long Short-Term Memory (LSTM) model to be learned with continuous data. After checking the behavioral recognition accuracy, it was confirmed that the accuracy of the "Lying Down" behavioral recognition results was high.