• Proceedings of the Korean Environmental Health Society Conference
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• 한국환경보건학회 2005년도 국제학술대회
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• pp.341-344
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• 2005

The expression of the glutathione S-transferase (GST), whose induction accounts for antioxidant defense system, is regulated by activation of CCAAT/enhancer binding protein ${\beta}$ ($C/EBP{\beta}$), Sick house syndrome (SHS) presents healthy damage owing to the indoor environment of a building. Toluene has been implicated in one of the important causes of SHS. The present study investigated the effects of toluene treatment on the induction of GSTA2 gene and its mechanism. H411E cells treated with toluene, and GSTA2 expression was determined by immunoblot analysis. The translocation of $C/EBP{\beta}$ was assessed by immunocytochemical assays. $C/EBP{\beta}$ DNA binding activity was determined by electrophoretic mobility shift assays. The role of the C/EBP binding site in the induction of the GSTA2 gene was assessed by luciferase reporter-gene activity. Toluene induced GSTA2 protein expression. In toluene-treated cells, $C/EBP{\beta}$ translocated to the nucleus and bound to the consensus sequence of C/EBP (TTGCGCAA). Toluene treatment increased luciferase reporter-gene activity in cells transfected with the C/EBP-containing regulatory region of the GSTA2 gene. Oxidative stress is believed to play an important role in the induction of GSTA2 gene by toluene This study shows that toluene-induced GSTA2 gene expression is dependent upon nuclear translocation and binding of $C/EBP{\beta}$ to the C/EBP response element in the GSTA2 gene promoter.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 한국식물병리학회 2004년도 The 2004 KSPP Annual Meeting & International Symposium
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• pp.65-67
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• 2004

During initial interactions of bacteria with their host plants, most plants recognize the bacterial infections and repel the pathogen by plant defense mechanism. The most active plant defense mechanism is the hypersensitive response (HR) which is the localized induced cell death in the plant at the site of infection by a pathogen. A primary locus induced in gram-negative phytopathogenic bacteria during this initial interaction is the Hrp locus. The Hrp locus is composed of a cluster of genes that encodes the bacteral Type 111 machinery that is involved in the secretion and translocation of effector proteins to the plant cell. DNA sequence analysis of hrp gene in phytopathogenic bacteria has revealed a Hrp pathogenicity is]and (PAI) with a tripartite mosaic structure. For many gram-negative pathogenic bacteria, colonization of the host's tissue depends on the type III protein secretion system (TTSS) which secrets and translocates effector proteins into the host cell. Effectors can be divided into several groups including broad host range effectors, host specific effectors, disease specific effectors, and effectors inhibit host defenses. The role of effectors carrying LRR domain in plant resistance is very elusive since most known plant resistance gene carry LRR domain. Host specific effectors such as several avr gene products are involved in the determination of the host specificity. Almost all the phytopathogenic Xanthomonas spp. carry avrBs1, avrBs2, and avrBs3 homologs. Some strains of X. oryzae pv. oryzae carry more than 10 copies of avrBs3 homologs. However, the functions of all those avr genes in host specificity are not characterized well.;

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• 제33권6호
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• pp.507-516
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• 2015

Debris flow is a representative natural disaster in Korea and occurs frequently every year. Recently, it has caused considerable damage to property and considerable loss of life in both mountainous and urban regions. Therefore, It is necessary to estimate the scope of damage for a large area in order to predict the debris flow. A response model such as the random walk model(RWM) can be used as a useful tool instead of a physics-based numerical model. RWM is a probability model that simplifies both debris flows and sedimentation characteristics as a factor of slopes for a subjective site and represents a relatively simple calculation method compared to other debris flow behavior calculation models. Although RWM can be used to analyzing and predicting the scope of damage caused by a debris flow, input variables for terrain conditions are yet to be determined. In this study, optimal input variables were estimated using DEM generated from the Aerial Photograph and LiDAR data of Mt. Umyeon, Seoul, where a large-scale debris flow occurred in 2011. Further, the deposition volume resulting from the debris flow was predicted using the input variables for a specific area in which the deposition volume could not be calculated because of work restoration and the passage of time even though a debris flow occurred there. The accuracy of the model was verified by comparing the result of predicting the deposition volume in the debris flow with the result obtained from a debris flow behavior analysis model, Debris 2D.

• Journal of Korean Society of Forest Science
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• 제94권2호통권159호
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• pp.73-81
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• 2005

This study was conducted to examine the geographic variation among provenances of Pinus densiflora in survival rate and height growth at four test plantations (Jungsun, Chungju, Naju, and Jeju). The plantations were parts of the eleven provenance trials of Pinus densiflora established by Korea Forest Research Institute in 1996. The survival rate and height growth were significantly different among test plantations at $p{\leq}0.01$. Latitude and longitude of test plantation were negatively correlated with survival rate and height growth. On the other hand, annual mean temperature, mean temperature (Nov.~Feb.), extremely low temperature (Dec.~Feb.), and annual mean growing days of test plantation were positively correlated with these two. The relationships between growth variables and geographic variables were analysed with canonical correlation analysis. A considerable amount of variation in survival rate and height growth was explained by latitude, annual mean growing days, extremely low temperature (Dec.~Feb.) and extremely high temperature (Nov.~Feb.) of provenances. It is estimated that up to 47.1% and 67.4% of the genetic variability in survival rate and height growth was attributable to the environmental variability of the provenances, respectively. The response surface curve of survival rate and height growth was plotted against latitude and longitude to examine growth performance of provenances for each test site. Generally, the local provenances showed better survival rate and height growth.

• Proceedings of the Korean Geotechical Society Conference
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.1118-1125
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• 2005

In this study, seismic safety of CFRD(Concrete-Face Rockfill Dam) type "D" dam in operation is evaluated from the results of 1-g shaking table test using similitude laws. Model dam is made by similitude law considering the grain size of prototype dam component. After the model dam is impounded to the normal water level(N.W.L), it is excited by artificial earthquake wave corresponding to standard design respond spectrum of the "D" dam site. Displacement response behavior of the dam is examined through the measurement of vertical and horizontal displacement of dam crest. Also, amplification characteristics of acceleration with dam height is examined through the measurement of acceleration with dam height. Finally, the purpose of this study is to evaluate seismic safety of "D" dam in operation. From the results of acceleration measurement, it was found that acceleration of dam crest was amplified about 1.52 times compared to the acceleration of dam bottom and amplification phenomenon is outstanding at three quarters of dam height from the bottom of dam. From the analysis of displacement behavior, it was estimated that vertical displacement of prototype dam is 6.8cm (0.1% of dam height) and horizontal displacement 12.3cm(0.2% of dam height). These percentages is much lower than 1% of dam height(general stability criteria). Therefore, it was concluded that seismic stability of "D" dam against an estimated earthquake is guaranteed.

• The Transactions of The Korean Institute of Electrical Engineers
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• 제64권2호
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• pp.228-231
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• 2015

In power system research fields, one of current key issues is the construction and commercialization of micro grid site which is called green island, carbon zero island, energy independent island, building micro grid, etc. and various affiliated technologies have been being vigorously developed to realize. In addition, various researches about electric vehicles (EVs) are in progress and it is expected to penetrate rapidly with the next a few years. Some new load models should be developed integrating with electric vehicle loads because the EVs' deployment could cause the change of load composition rate on power system planning and operations. EVs are also resources for micro grid as well as distributed generation and demand response so that various supply and demand side resources should be considered for micro grid researches. In this paper, the load composition rate of residential sectors is prospected considering the deployment of EVs and the resource configuration of micro grid is optimized based on net present cost. In the optimization, the load patten of case studies includes EV's charging characteristics and various cases are simulated comparing micro grid environment and normal condition. HOMER is used to compare various cases and economic effects.

• BMB Reports
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• 제53권3호
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• pp.142-147
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• 2020

Lipid accumulation in white adipose tissue is the key contributor to the obesity and orchestrates numerous metabolic health problems such as type 2 diabetes, hypertension, atherosclerosis, and cancer. Nonetheless, the prevention and treatment of obesity are still inadequate. Recently, scientists found that brown adipose tissue (BAT) in adult humans has functions that are diametrically opposite to those of white adipose tissue and that BAT holds promise for a new strategy to counteract obesity. In this study, we evaluated the potential of sinapic acid (SA) to promote the thermogenic program and lipolysis in BAT. SA treatment of brown adipocytes induced the expression of brown-adipocyte activation-related genes such as Ucp1, Pgc-1α, and Prdm16. Furthermore, structural analysis and western blot revealed that SA upregulates protein kinase A (PKA) phosphorylation with competitive inhibition by a pan-PKA inhibitor, H89. SA binds to the adenosine triphosphate (ATP) site on the PKA catalytic subunit where H89 binds specifically. PKA-cat-α1 gene-silencing experiments confirmed that SA activates the thermogenic program via a mechanism involving PKA and cyclic AMP response element-binding protein (CREB) signaling. Moreover, SA treatment promoted lipolysis via a PKA/p38-mediated pathway. Our findings may allow us to open a new avenue of strategies against obesity and need further investigation.

• Journal of the Korea Safety Management & Science
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• 제11권2호
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• pp.111-115
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• 2009

As shown in the accident analysis from 2007, it has been found that causes of accidents on large-scale projects are different from those found in general construction projects. A 300-question survey regarding systematic and practical aspects of safety problems at construction sites was distributed to ten (10) different companies. Participants were to respond subjectively, so that the results could be used to assemble the first formal questionnaire survey. They were collected and compiled by an advisory committee for this study. The resulting surveys were then sent to the division chiefs of the top 100 construction companies in Korea, in order to improve the response rate. The Department of Safety & Health Direction, and the :Ministry of Labor, ROK sent the same sheets to medium and small construction companies that placed within 101-200th of all Korea construction companies. The above safety engineers were classified into four (4) levels, from 1st to 4th, followed by the project dollar amount and risk level. Formulae were developed to assign safety engineers to construction sites by engineer level, the project dollar amount, and the project risk level. Conclusions are summarized as follows: 1) Reviewing the assignment system of experienced safety engineers to large scaled projects - The more experienced the engineers assigned to a project, the higher the level of accident prevention. 2) Enforcing the assignment of advanced level safety engineers to large-scaled sites - At least one advanced-level safety engineer should be assigned to construction sites with projects valued at $15million USD (15,000,000,000). 3) For assigning safety engineers by risk level - Twenty models have been developed to calculate the number of safety engineers to be assigned by risk level. In the future, risk level for each job should be established by the government (as is now the practice in Germany).

• Korean Journal of Environmental Biology
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• 제26권3호
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• pp.214-219
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• 2008

Changes in stress-associated biomolecules can be used as an important criterion for assessing the levels of environmental pollution because living organisms demonstrate contamination-stimulated stress responses. This study was conducted to determine the environmental status of Masan-Jinhae Bay, Korea, and its effects on marine organisms by investigating the endoplasmic reticulum (ER) dysfunction in the organs of the flat fish, Limanda yokohamae. ER dysfunction was evaluated via Western blot analysis of the ER stress proteins, immunoglobulin heavy chain binding protein (BiP) and C/EBP-homologous protein (CHOP), and the ER stress-associated protein caspase-12. The results showed that the amount of BiP and CHOP immunoreactivity in the flat fish from the bay area was much greater than that from the Gangneung, as a reference site. Similar to the ER stress proteins, the immunoreactivity of caspase-12 was also found to be elevated in the bay area when compared with that of Gangneung. These data suggest that the environmental status of Masan-Jinhae Bay induces the ER stress response, which is able to lead to phenotypic changes in marine organisms including fish.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• 제28권9호
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• pp.698-706
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• 2017

The risk of high power electromagnetic(HPEM) pulse exposure to the devices used in digital control system such as PLC(programmable logic controller) and communication cable is increasing. In this paper, two different frequency ranges HPEMs were exposed to those control systems to assess the each vulnerability. The vulnerability of the EUTs exposed from HPEM were analyzed and compared with a variation of distances and source power. As the EUTs were exposed to higher level of HPEM, the voltage and communication waveform of the control system had shown a distorted response. And the unshielded twisted pair(UTP) cable connected to the EUTs showed operation failures with induced voltage. However, the foiled twisted pair(FTP) cable shielded the connected device efficiently from the HPEM exposure. Therefore, the necessity of the protection measures against the vulnerability of HPEM exposure for the digital control system used in power facilities and industrial site were verified.