• 한국축산식품학회지
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• 제18권1호
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• pp.19-26
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• 1998

In order to study the tenderizing effect of the proteolytic enzyme, ficin, from fig fruit (Ficus carica L), the enzyme was purified from fig latex by precipitation and chromatography. The ficin separated from Bongraesi showed single band on SDS-PAGE. However, the ficin from Masui showed tow bands. The specific activity of ficin purified from Bongraesi species was 2.8 unit/mg protein and that from Masui species was 6.5 unit / mg protein. The amounts of ficin purified from 50 mL of crude latex of Bongraesi and Masui were 1,760 mg and 657 mg, respectively. the water holding capacity of beef decreased to the large extent, when sugar Bongraesi latex and Masui latex were added. The hardness of beef showed decreasing tendency with the time, however, after 60 min, it decreased and thereafter increased a little after 120 min. the hardness of beef decreased sharply with addition of the latex of Bongraesi and Masui. The Masui has more tenderizing effect than the Bongraesi. When meat was mixed with tenderizing agent(ficin) and not heated, the change of color showed significant difference (p＜0.01). when meat was mixed with tenderizing agent(ficin) and heated, the toughness showed significant difference (p＜0.01) and the softness showed significant difference (p＜0.001).

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1184-1190
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• 2009

A thermostable extracellular $\beta$-mannanase from the culture supernatant of a fungus Aspergillus niger gr was purified to homogeneity. SDS-PAGE of the purified enzyme showed a single protein band of molecular mass 66 kDa. The $\beta$-mannanase exhibited optimum catalytic activity at pH 5.5 and $55^{\circ}C$. It was thermostable at $55^{\circ}C$, and retained 50% activity after 6 h at $55^{\circ}C$. The enzyme was stable at a pH range of 3.0 to 7.0. The metal ions $Hg^{2+}$, $Cu^{2+}$, and $Ag^{2+}$ inhibited complete enzyme activity. The inhibitors tested, EDTA, PMSF, and 1,10-phenanthroline, did not inhibit the enzyme activity. N-Bromosuccinimide completely inhibited enzyme activity. The relative substrate specificity of enzyme towards the various mannans is in the order of locust bean gum>guar gum>copra mannan, with $K_m$ of 0.11, 0.28, and 0.33 mg/ml, respectively. Since the enzyme is active over a wide range of pH and temperature, it could find potential use in the food-processing industry.

• 한국인터넷방송통신학회논문지
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• 제14권3호
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• pp.69-74
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• 2014

범죄발생 예방 및 도로상 교통 상황파악 등 각종 요구로 인하여 각 기관 및 지자체 마다 CCTV설치가 이루어져 우리 주변의 많은 곳에서 CCTV를 살펴볼 수 있다. 이렇게 기존에 설치된 CCTV의 공간적 분포도만의 단면적 분석자료로는 향후 신규로 설치될 CCTV의 위치 선정이나 제한된 예산으로 이루어져야 할 시스템 구축에 명확한 기준을 줄 수 없다. 또한 각각의 기관마다 내부적으로 고유의 업무 기능 수행결과로 구축된 자료인 DataBas들이 많이 있지만 이를 다른 목적을 위해 통합적으로 분석하는 경우는 빈번하지 않다. 매시업(Mashup)은 별도로 존재하는 정보와 서비스를 융합하여 새로운 서비스, 데이터베이스 등을 만드는 것을 말한다. 이에 본 연구는 지역의 교통 환경, 기설치된 CCTV위치, 방범 등의 기관 내부 DB를 매쉬업기능을 통하여 안양시를 중심으로 하여 새로운 CCTV 설치 위치를 결정하는 방법을 제공한다.

• 한국ITS학회 논문지
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• 제8권3호
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• pp.27-33
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• 2009

기존의 교통상황판운영에 사용하는 지도는 2D를 기본으로 하는 전자지도를 중심으로 표준노드링크의 속성을 반영하는 형태이다. 2D형태의 교통전자지도는 그래픽형식에 운영자에게 실시간으로 교통상황을 직관적으로 판단하는데 도움을 제공하였으나 2D형식이라는 한계가 존재할 수밖에 없었다. 점차적으로 IT기술의 고도화, 하드웨어, 통신기술의 발달 등으로 과거에 다룰수 없었던 대용량데이터처리가 원활해지고, 다양한 도로이용자의 고급화된 교통수요에 대응하기 위해서는 점차적으로 교통관리자나 운영자들이 교통정보관련 장비들이나 운영시나리오에 대해 다각적으로 분석을 할 수 있는 방안이 강구되어야 한다. 기존의 교통상황판은 점, 선, 면 형식의 2D전자지도를 기반으로 그 위에 교통소통상황 등의 부가정보를 표현하였으나, 본 연구에서는 구글어스의 API를 활용하도록 한다. 구글어스는 고해상도의 위성사진과 이를 이용한 3D화면을 표출서비스를 제공하며, 이를 기반으로 교통소통정보, 버스노선 및 정류장 제보, 3D 객체 표현 등의 콘텐츠와 야후 POI(야후 거기)를 Mash-Up하여 보다 현실적인 교통상황정보를 제공하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1196-1206
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• 2014

A metagenomic fosmid library was constructed using genomic DNA isolated from the gut microflora of Hermetia illucens, a black soldier fly. A cellulase-positive clone, with the CS10 gene, was identified by extensive Congo-red overlay screenings for cellulase activity from the fosmid library of 92,000 clones. The CS10 gene was composed of a 996 bp DNA sequence encoding the mature protein of 331 amino acids. The deduced amino acids of CS10 showed 72% sequence identity with the glycosyl hydrolase family 5 gene of Dysgonomonas mossii, displaying no significant sequence homology to already known cellulases. The purified CS10 protein presented a single band of cellulase activity with a molecular mass of approximately 40 kDa on the SDS-PAGE gel and zymogram. The purified CS10 protein exhibited optimal activity at $50^{\circ}C$ and pH 7.0, and the thermostability and pH stability of CS10 were preserved at the ranges of $20{\sim}50^{\circ}C$ and pH 4.0~10.0. CS10 exhibited little loss of cellulase activity against various chemical reagents such as 10% polar organic solvents, 1% non-ionic detergents, and 0.5 M denaturing agents. Moreover, the substrate specificity and the product patterns by thin-layer chromatography suggested that CS10 is an endo-${\beta}$-1,4-glucanase. From these biochemical properties of CS10, it is expected that the enzyme has the potential for application in industrial processes.

• 한국정보통신학회논문지
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• 제21권11호
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• pp.2153-2166
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• 2017

웹 앱에는, 서로 다른 웹 페이지를 웹브라우저에 로드할 때 동일 데이터가 반복적으로 획득되고 처리되어 화면상에 표시되는 문제가 있다. 본 논문은 이 문제를 해결하기 위해 자바 웹 앱을 재구조화하는 기법을 제안하고 평가한다. 자바 웹 앱을 MVC(Model-View-Controller) 아키텍처 관점에서 동적 분석하고 컴포지트 뷰 패턴을 활용하여 중복해서 사용되는 데이터를 식별한다. 이를 토대로 페이지 요청 시 중복 데이터가 로드되지 않도록 앱을 재구조화한다. 재구조화를 통해 MVC 아키텍처에 부합하며 성능이 향상된 웹 앱을 생성한다. 이렇게 재구조화된 웹 앱은 기존 웹 앱의 응답 시간을 데스크톱 PC, 모바일 기기에서 각각 38%, 55% 개선하였다. 아울러 오픈 소스 웹 앱을 이용한 사례 연구를 통해 제안하는 기법의 적용 가능성을 보였다.

• 한국정보시스템학회지:정보시스템연구
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• 제26권2호
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• pp.1-23
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• 2017

Purpose Facebook is a social network service that has the highest number of Monthly Active Users around the world. Hence, marketers have selected Facebook as the most important platform to get customer engagement. With respect to the customer engagement enhancement, the most popular and engaging post type in the Facebook brand fan pages related to what was usually classified as 'sweepstakes'. Sweepstakes refer to a form of gambling where the entire prize may be awarded to the winner. Which makes customers more engaged with the brand. This study aims to explore sweepstakes-oriented social media marketing approaches based on the application of big data analytics. Design/methodology/approach we collect sweepstakes data from each company based on the data crawling from the Facebook brand fan pages. The output of this study explains how companies in each category of FCB grid can design and apply sweepstakes for their social media marketing. Findings The results show that they have one thing in common across the four quadrants of FCB grid. Regardless of the quadrants, most frequently observed type is 'Simple/Quiz or Comments/Quatrains [event type of sweepstakes] + Gifticon [type of reward prize] + Image [type of message display] + No URL [Link toother website] +Single-Gift-Offer [type of reward prize payment]'. So, if the position of the brand is hard to be defined by the FCB grid model, then this general rule can be applied to all types of brands. Also some differences between the quadrants of the FCB grid were observed. This study offers several research implications by analyzing Sweepstakes-oriented social media marketing approaches in Facebook brand fan pages. By using the FCB grid model, this study provides guidance on how companies can design their sweepstakes-oriented social media marketing approaches in the context of Facebook brand fan pages by considering their context.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.545-555
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• 2014

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

• 한국시뮬레이션학회논문지
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• 제26권2호
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• pp.9-17
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• 2017

현대사회의 다양한 분야에서는 복잡한 사회현상에 대하여 문제를 정의하고 문제해결책의 결과를 분석하는 과정으로 모델링 및 시뮬레이션을 활용하고 있다. 나날이 사회현상의 문제가 복잡해짐에 따라 요구되는 모델링 및 시뮬레이션 환경의 계산자원의 요구사항도 높아지고 있다. 이러한 요구사항을 수용하기 위해서 클라우드 서비스 등과 같이 컴퓨팅 자원을 유동적으로 사용할 수 있는 환경이 대두되었다. 본 연구에서는 이러한 컴퓨팅 자원 활용 시스템을 보다 효율적으로 활용할 수 있는 웹 기반 재구성이 가능한 시뮬레이션 실험 틀을 제안한다. 제안하는 시뮬레이션 실험 틀은 다양한 분산 컴퓨팅 환경을 지원할 수 있도록 프론트엔드(Front-end)에서 웹을 활용하여 시뮬레이션 모델의 재구성 시스템을 구축하고 백엔드(Back-end) 이산 사건 시뮬레이션의 실행을 담당하는 이산사건 시뮬레이션 실행단으로 구성된다. 본 연구는 사례연구를 통해 분산형 시뮬레이션 환경이 단일 시뮬레이션 환경보다 시간적 효율이 더 높음을 확인하였다.

• 대한임베디드공학회논문지
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• 제14권2호
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• pp.87-96
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• 2019

Low power issue is one of the most critical problems in the Internet of Things (IoT), which are powered by battery. To solve this problem, various approaches have been presented so far. In this paper, we propose a method to reduce the power consumption by reducing the numbers of accesses into the flash memory consuming a large amount of power for on-chip software execution. Our approach is based on using cooperative logging structure to distribute the sampling overhead in single sensor node to adjacent nodes in case of rare-event applications. The proposed algorithm to identify event occurrence is newly introduced with negative feedback method by observing difference between past data and recent data coming from the sensor. When an event with need of flash access is determined, the proposed approach only allows access to write the sampled data in flash memory. The proposed event detection algorithm (EDA) result in 30% reduction of power consumption compared to the conventional flash write scheme for all cases of event. The sampled data from the sensor is first traced into the random access memory (RAM), and write access to the flash memory is delayed until the page buffer of the on-chip flash memory controller in the micro controller unit (MCU) is full of the numbers of the traced data, thereby reducing the frequency of accessing flash memory. This technique additionally reduces power consumption by 40% compared to flash-write all data. By sharing the sampling information via LoRa channel, the overhead in sampling data is distributed, to reduce the sampling load on each node, so that the 66% reduction of total power consumption is achieved in several IoT edge nodes by removing the sampling operation of duplicated data.