• Journal of the Korean Institute of Telematics and Electronics A
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• v.32A no.8
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• pp.31-38
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• 1995

In this paper, we consider the leaky bucket parameter dimensioning problem in the presence of the cell delay variation(CDV) which arises at the customer premises network dud to the multiplexing with other traffic streams. We consider an ATM multiplexer in which a single CBR stream and several heterogeneous VBR traffic streams are multiplexed. Choosing an MMPP model for the bursty traffic streams, we derive an (MMPP+DD)/D/1/K queueing model for the evaluation of the CDV experienced by the CBR stream. We first evaluate the equilibrium queue length distribution embedded at tagged-cell arrival-time instants, based on whcih we calcuate the inter-cell time distribution and the distribution kof the number of tagged-cell departures in an arbitrary interval. Then we apply the analysis to the dimensionging problem of the leaky bucket parameters, examining how the employed traffic model affects the determination of the bucket size. Through numerical examples, we confirm that the Poisson traffic model can underestimate the bucket size, thus causing a considerable blocking probability for compliant use cells while the MMPP model can optimally design the bucket size which keeps the blocking probability under the target value.

• Journal of Hydrogen and New Energy
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• v.19 no.4
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• pp.283-290
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• 2008

In this paper, experiments with an air-breathing proton exchange membrane fuel cell (PEMFC) for mobile devices were carried out according to cathode conditions. These conditions are defined by the cathode flow field plate type (the channel type, the open type) and the cathode surface direction. Single-cell and 6-cell stack were used in the experiments. The experimental results showed that the open-type cathode flow field plate gave a better performance than the small channel type. In the experiments related to the direction of the slits on the cathode flow field plate, the horizontal slit cell was better than the vertical one. With respect to the cathode surface direction, when the cathode surface is placed in the direction normal to the ground, the PEMFC generated more stable power in the mass transport loss region. Since stable power in the mass transport region is closely related to the air supply, computational fluid dynamics (CFD) analysis for air-breathing PEMFC of different cathode surface directions was performed.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.72-78
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• 2001

It is possible ot prepare protoplasts of the zygomycete fungus, Phycomyces blakesleeanus, by digesting the cell wall of spore germlings with commercially available chitinase and chitosanase. However, the cells without any cell walls immediately form large aggregates, and thus, it is difficult to isolate the individually separated protoplasts. Inherent problem with the formation of aggregates in preparing protoplasts could be solved by the use of bovine serum albumin (BSA). As a result, we were able to prepare a large number of single protoplsts quickly and easily. We took time-lapse photomicrographs of the formation of protoplasts, and found that there were certain regions of the cell wall of spore germlings that were sensitive to chitinase and chitosanase, although the cell wall of the original spores is known to be insensitive to these enzymes. There are two kinds of cell walls on a spore germling; one with a bound wheat germ agglutinin (WGA), and the other a bound concanavalin A (ConA). Furthermore, only cells with walls which had bound WGA were able to regenerate, while those with walls with bound ConA were not able to regenerate.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.12
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• pp.2328-2339
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• 1994

The PBS(Partial Buffer Sharing) space priority mechanism is one of priority control methods which may improve the performance of a single server queueing system when mixed traffic with different performance requirements is applied to the system. This paper analyzes the cell loss behavior of PBS assuming loss sensitive traffic and delay sensitive traffic are applied to the system. To derive the successive cell loss probabilities. which are an important performance measure of realtime traffic, we develop a recursive algorithm. Performance results show the successive cell loss probabilities obtained by our method are lager than the probabilities derived from an independent cell loss assumption. These results may indicate the limitation of PBS for realtime traffic and the increase of the admissible load with the criterion of quality of service.

• BMB Reports
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• v.42 no.8
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• pp.516-522
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• 2009

SH2D4A, comprising a single SH2 domain, is a novel protein of the SH2 signaling protein family. We have previously demonstrated SH2D4A is expressed ubiquitously in various tissues and is located in the cytoplasm. In this study we investigated the function of SH2D4A in human embryonic kidney (HEK) 293 cells using interaction analysis, cell proliferation assays, and kinase activity detection. SH2D4A was found to directly bind to estrogen receptor $\alpha$ (ER$\alpha$), and prevent the recruitment of phospholipase C-$\gamma$ (PLC-$\gamma$) to ER$\alpha$. Moreover, we observed its inhibitory effects on estrogen-induced cell proliferation, involving the protein kinase C (PKC) signaling pathway. Together, these findings suggested that SH2D4A inhibited cell proliferation by suppression of the ER$\alpha$/PLC-$\gamma$/PKC signaling pathway. SH2D4A may be useful for the development of a new anti-cancer drug acting as an ER signaling modulator.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.1
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• pp.17-21
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• 2005

The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR. Porcine embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% $CO_2$ and 20% $O_2$ at $37^{\circ}C$ in NCSU23 medium. Melatonin was added to medium at concentration of 1nM, 5 nM, and 10 nM. When treated with 1nM (39.0%) of melatonin, the developmental rate of embryos beyond the morula stage were higher than that of control group (31.0%) (p<0.05). Number of inner cell mass and tropectoderm cell in control (23.0${\pm}$0.5 and 17.3${\pm}$0.8), 1 nM (23.6${\pm}$0.6 and 19.0${\pm}$0.5), and 5 nM (23.3${\pm}$1.1 and 16.3${\pm}$0.8) treated with melatonin were higher than in 10 nM (20.0${\pm}$0.5 and 13.3${\pm}$0.8) treated with melatonin (p<0.05). To develop an mRNA phenotypic map for the expression of catalase, bax and caspase-3, single cell RT-PCR analysis were carried out in porcine IVM/IVF embryo. Catalase was detected in 0, 1 and 5 nM supplemented with melatonin, but bax and caspase-3 were detected in 10 nM treated with melatonin.

• Tuberculosis and Respiratory Diseases
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• v.87 no.4
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• pp.494-504
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• 2024

Background: Ubiquitin C-terminal hydrolase L1 (UCHL1), which encodes thiol protease that hydrolyzes a peptide bond at the C-terminal glycine residue of ubiquitin, regulates cell differentiation, proliferation, transcriptional regulation, and numerous other biological processes and may be involved in lung cancer progression. UCHL1 is mainly expressed in the brain and plays a tumor-promoting role in a few cancer types; however, there are limited reports regarding its role in lung cancer. Methods: Single-cell RNA (scRNA) sequencing using 10X chromium v3 was performed on a paired normal-appearing and tumor tissue from surgical specimens of a patient who showed unusually rapid progression. To validate clinical implication of the identified biomarkers, immunohistochemical (IHC) analysis was performed on 48 non-small cell lung cancer (NSCLC) tissue specimens, and the correlation with clinical parameters was evaluated. Results: We identified 500 genes overexpressed in tumor tissue compared to those in normal tissue. Among them, UCHL1, brain expressed X-linked 3 (BEX3), and midkine (MDK), which are associated with tumor growth and progression, exhibited a 1.5-fold increase in expression compared to that in normal tissue. IHC analysis of NSCLC tissues showed that only UCHL1 was specifically overexpressed. Additionally, in 48 NSCLC specimens, UCHL1 was specifically upregulated in the cytoplasm and nuclear membrane of tumor cells. Multivariable logistic analysis identified several factors, including smoking, tumor size, and high-grade dysplasia, to be typically associated with UCHL1 overexpression. Survival analyses using The Cancer Genome Atlas (TCGA) datasets revealed that UCHL1 overexpression is substantially associated with poor survival outcomes. Furthermore, a strong association was observed between UCHL1 expression and the clinicopathological features of patients with NSCLC. Conclusion: UCHL1 overexpression was associated with smoking, tumor size, and high-grade dysplasia, which are typically associated with a poor prognosis and survival outcome. These findings suggest that UCHL1 may serve as an effective biomarker of NSCLC.

• Journal of Biomedical Engineering Research
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• v.33 no.4
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• pp.169-176
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• 2012

Tumor cell morphology is closely related to its migratory behaviors. An active tumor cell has a highly irregular shape, whereas a spherical cell is inactive. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use 3D time-lapse phase-contrast microscopy to analyze single cell morphology because it enables to observe long-term activity of living cells without photobleaching and phototoxicity, which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we calculated the Fourier descriptor that morphological characteristics of cell to classify tumor cells into active and inactive groups. We validated classification accuracy by comparing our findings with manually obtained results.

• Journal of IKEEE
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• v.1 no.1 s.1
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• pp.11-18
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• 1997

By utilizing the technique to monitor the DC cell node voltages through circuit simulation, degradation of the static read operating margin In high load-resistor SRAM cell was examined, which is caused by parasitic resistances and transistor asymmetries in this cell structure. By selectively adding the parasitic resistances to an ideal cell, the influence of each parasitic resistance on the operating margin was examined, and then the cases with parasitic resistances in pairs were also examined. By selectively changing the channel width of cell transistors to generate cell asymmetry, the influence of cell asymmetry on the operating margin was also examined. Analyses on the operating margins were performed by comparing the supply voltage values at which two cell node voltages merge to a single value and the differences of cell node voltages at VDD=5V in the simulated node voltage characteristics. By determining the parasitic resistances and the transistor asymmetries which give the most serious effect on the static read-operation of SRAM cell from this analysis based on circuit simulated, a criteria was provided, which can be referred in the design of new SRAM cell structures.

• Journal of Broadcast Engineering
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• v.18 no.2
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• pp.215-224
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• 2013

With the rising popularity of smart-phones, the supply of Internet protocol television is largely increased and it causes the wireless communication network load to be increased. To overcome such an overloading problem, 3GPP is now working on the standardization of MBMS since LTE Release 6 specification. MBMS has good performance in bandwidth efficiency by sharing the same bandwidth with the mulitple MBMS users having the same content. According to the proposed algorithm, in heterogeneous networks having a dispersed Single-cell MBMS where 3GPP network and Wi-Fi network are mixed, if the number of MBMS users, who are belonging to cells supporting a dispersed Single-cell MBMS, is more than a specified threshold, the MBMS priority policy is operated. Otherwise, the Wi-Fi priority policy is executed. As the simulation results show both the increase of total available bandwidth ratio and the decrease of network usage cost, it is confirmed that the proposed scheme allows the network efficiency to be maximized.