• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.96-98
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• 2006

Proper water management is vital to achieve high performance and durability of PEFC (Polymer Electrolyte Fuel Cell). The effects of the residual water from PEFC after purge in shut-down processes on GDL/MEAs were investigated with freeze/thaw cycles Freeze/thaw cycle tests were conducted with single cells which were designed from transparent acryl plates. Single cells which contain several amount of residual water were cycles from $80^{\circ}C$ to $-28^{\circ}C$. The resistance changes of the single cells which have various amount of residual water were evaluated by ac-impedance analysis with 24 times of freeze/thaw cycles. Also, after the freeze/thaw cycles, the property changes were characterized by visual methods such as SEM, EPMA. Though it was difficult to observe noticeable property changes in the visual characterizations, the resistance of cells dramatically increased with the amount of remained water.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2005년도 추계학술대회 논문집
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• pp.229-234
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• 2005

Thin-walled multicell box girders subjected to an eccentric load can he produced the three global behaviors of flexure, torsion, and distortion. Specially in railway bridges subjected to much eccentric load, it is quite important to evaluate influences of torsion and distortion. But it is very difficult to evaluate each influences of major behaviors numerically. If we can decompose an eccentric load P into flexural, torsional, and distortional forces. we can execute quantitative analysis each influences of major behaviors. Decomposition of Applied Load for Thin-walled Rectangular multi-cell box girders is reserched by Park, Nam- Hoi(Development of a multicell Box Beam Element Including Distortional Degrees of Freedom, 2003). But researches about trapezoidal multi-cell section is insufficient. So, this paper deals with multi-cell trapezoidal box girders. An expanded method, which is based on the force decomposition method for a single cell box girder given by Nakai and Yoo, is developed herein to decompose eccentric load Pinto flexural, torsional, and distortional forces. Derive formulas by decomposition of eccentric load is verified by 3D shell-modelling numerical analysis.

• 농업과학연구
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• 제49권4호
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Korean Chemical Engineering Research
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• 제50권4호
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• pp.743-748
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• 2012

본 논문에서는 미세 환경이 Pseudomonas aeruginosa의 운동성에 주는 영향을 조사하기 위하여 다양한 크기의 미세유로 내에서 박테리아의 운동성을 분석하였다. 본 논문에서는 미세유체 칩을 사용하여 2차원 공간을 만들며, $10{\sim}100{\mu}m$ 너비의 채널 안에서 단일 박테리아의 운동 변수인 이동속도, 'run'운동 지속시간, 'tumble' 각도를 측정하였고 각 미세유로 내에서 박테리아의 운동을 표현할 수 있는 물리적 상수인 random motility coefficient를 구하였다. 상기의 물리적 측정치를 분석한 결과, 박테리아는 공간제약이 있는 경우 편모의 운동이 채널의 벽의 영향으로 인하여 회전 운동에 영향을 받게 되고, 'run' 운동 지속 시간이 짧아지는 것을 확인하였다. 따라서, 공간의 제한이 박테리아의 운동성을 감소시킴을 알 수 있었다. 본 연구의 결과는 박테리아의 운동성을 쉽고 정확하게 분석할 수 있는 측정 방법으로 널리 활용될 것으로 기대된다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2006년도 춘계종합학술대회
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• pp.927-930
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• 2006

마이크로프로세서 제조업체의 대명사로 불리는 인텔이 비메모리 시장을 거의 독점하고 있는 가운데, IBM, Sony, Toshiba 인 세 개의 기업이 도전적으로 힘을 합하여 차세대 엔터테인먼트 프로세서라고 불리는 멀티코어 기반의 Cell 프로세서를 개발하였다. Cell 프로세서는 기존의 Power 라는 아키텍쳐를 기반으로 하는 코어 PPE (Power processor Element)를 중심으로 8개의 데이터 처리용 코어 SPE(Synergistic processor Element)를 포함하고 있으며, SIMD(Single Input Multiple Data) 데이터 처리 방식을 지원하여 데이터 처리량이 많은 멀티미디어나 게임 어플리케이션을 처리하는데 최적화되도록 설계된 프로세서로서, 기본적으로는 Power 마이크로프로세서 아키텍쳐 및 구조에 대한 혁신적인 확장 기능을 포함하고 있다. 본 논문에서는 현존하는 프로세서 중 가장 성능이 우수하다고 평가받는 Cell 마이크로프로세서의 설계 개념과 그 구조에 대하여 분석한다.

• IMMUNE NETWORK
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• 제13권2호
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• pp.43-54
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• 2013

Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells modulating the immune network. These advances significantly support highthroughput and high-content analyses and enable an integrated understanding of the cellular and molecular interactions that underlie complex biological systems.

• Applied Microscopy
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• 제46권3호
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• pp.134-139
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• 2016

Detailed structural and molecular imaging of intact organs has incurred academic interest because the associated technique is expected to provide innovative information for biological investigation and pathological diagnosis. The conventional methods for volume imaging include reconstruction of images obtained from serially sectioned tissues. This approach requires intense manual work which involves inevitable uncertainty and much time to assemble the whole image of a target organ. Recently, effective tissue clearing techniques including CLARITY and ACT-PRESTO have been reported that enables visualization of molecularly labeled structures within intact organs in three dimensions. The central principle of the methods is transformation of intact tissue into an optically transpicuous and macromolecule permeable state without loss of intrinsic structural integrity. The rapidly evolving protocols enable morphological analysis and molecular labeling of normal and pathological characteristics in large assembled biological systems with single-cell resolution. The deep tissue volume imaging will provide fundamental information about mutual interaction among adjacent structures such as connectivity of neural circuits; meso-connectome and clinically significant structural alterations according to pathologic mechanisms or treatment procedures.

• Animal cells and systems
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• 제7권2호
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• pp.159-164
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• 2003

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2.8 kb was detected by Northern blot analysis, The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV-inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60％ identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.

• Structural Engineering and Mechanics
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• 제78권3호
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• pp.255-267
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• 2021

The analysis of simply supported single-cell skew-curved reinforced concrete (RC) box-girder bridges is carried out using a finite element based CsiBridge software. The behaviour of skew-curved box-girder bridges can not be anticipated simply by superimposing the individual effects of skewness and curvature, so it becomes important to examine the behaviour of such bridges considering the combined effects of skewness and curvature. A comprehensive parametric study is performed wherein the combined influence of the skew and curve angles is considered to determine the maximum bending moment, maximum shear force, maximum torsional moment and maximum vertical deflection of the bridge girders. The skew angle is varied from 0° to 60° at an interval of 10°, and the curve angle is varied from 0° to 60° at an interval of 12°. The scantly available literature on such bridges focuses mainly on the analysis of skew-curved bridges under dead and point loads. But, the effects of actual loadings may be different, thus, it is considered in the present study. It is found that the performance of these bridges having more curvature can be improved by introducing the skewness. Finally, several equations are deduced in the non-dimensional form for estimating the forces and deflection in the girders of simply supported skew-curved RC box-girder bridges, based upon the results of the straight one. The developed equations may be helpful to the designers in proportioning, analysing, and designing such bridges, as the correlation coefficient is about 0.99.

• 한국전기전자재료학회논문지
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• 제24권9호
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• pp.744-749
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• 2011

Recently, annual usage of energy is dramatically increasing because industrialization is going faster and more electricity is needed due to various electronic devices. This study focused on the performance characteristics of solar cell using the impedance technique. The experiment measured an impedance according to frequency's from 2 mHz until 1 MHz. It could know that the impedance was decreased according to the frequency increases in solar cell. The imaginary part was changed from capacitance component to inductance component.