• 제목/요약/키워드: Single-cell Measurement

검색결과 115건 처리시간 0.033초

Single Chip Microcomputer를 이용한 탁주발효(濁酒醱酵)의 자동계측(自動計測)과 제어방법(制御方法)의 개발(開發) (Development of Automatic Measurement and Control Method based on Single Chip Microcomputer for Tackjoo Fermentation)

  • 김경만;전재근
    • 한국식품과학회지
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    • 제25권4호
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    • pp.391-394
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    • 1993
  • 탁주발효의 자동화를 위하여 발효 중 발생하는 기체를 측정할 수 있는 bubble counter와 제어장치를 개발하였다. 동 장치를 활용하여 pilot 규모의 탁주발효에 적용하였다. 탁주발효중 발생하는 기체를 계측하는 bubble counter는 photo-interruptor와 acryl관으로 구성하였으며, 계측되는 기체용적은 기포당 0.018ml였다. 그리고 탁주발효의 제어장치는 single chip microcomputer(MC68705R3)로 제작하였으며, 발효액의 온도를 측정, 제어하기 위하여 온도계측회로를 제작하였고, 냉각수 순환을 결정하는 solenoid valve의 작동으로 온도제어를 하였다. 동 장치를 운영하기 위하여 software를 작성하여 ROM에 구조화하였다. 동 장치로 $CO_2$ 발생량과 속도를 자동적으로 추정한 결과 알코올 농도곡선과 형태가 서로 유사함을 보여주었으며, $30^{\circ}C$ 발효조건에서 24시간때에 최대 발생량을 보였다. 알코올농도와 $CO_2$ 발생량은 뛰어난 상관관계를 보였으며, 이로부터 제어알고리듬을 작성할 수 있었다.

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반도체레이져를 이용한 온도 및 농도의 계측 (Temperature and Concentration measurement using Semi-conductor diode laser)

  • 정대헌;노동순;지전유이
    • 한국연소학회:학술대회논문집
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    • 한국연소학회 2000년도 제21회 KOSCO SYMPOSIUM 논문집
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    • pp.168-174
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    • 2000
  • A diode laser sensor system based on absorption spectroscopy techniques has been developed to measure $CO_2$ concentration and temperature non-intrusively in high temperature combustion environments using a 2.0 ${\mu}m$ DFB(Distributed Feedback) laser. Two optics was fabricated in pig-tail fashion and all optical components were implemented in a single box. The evolution of measurement sensitivity was done using test cell by changing sweep frequency and $CO_2$ concentration. Gas temperature was determined from the ratio of integrated line strengths. Species concentration was determined from the integrated line intensity and the measured temperature. The result show that the system has 2% error in wide operation frequency range and accuracy of $CO_2$ concentration was about 3%. The system was applied to measure temperature and concentration in the combustion region of a premixed $CH_4$ +air triangular flame. The measurement results of gas temperature agreed well with thermocouple results. Many considerations were taken into account to reduce optical noise, etalon effect, beam steering and base line matching problem. The evaluations results and actual combustion measurement demonstrate the practical and applicability for in-situ and real time combustion monitoring in a practical system.

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광계측 시스템을 이용한 유.기중 코로나 방전의 전계변화 측정 및 비교분석 (Analysis and the measurement of the variation of electric field in air and oil using optical measuring system)

  • 마지훈;류철휘;강원종;장용무;구자윤
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2002년도 하계학술대회 논문집 C
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    • pp.1668-1670
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    • 2002
  • Since more than two decades, the conventional PD detecting systems have been employed in order to detect the partial discharges occurring inside the HV power apparatus for their diagnosis by use of different type of detection such as acoustic and UHF detection method. Regardless of their wide on-site application, a certain number of technical inconveniences have been disclosed as follows : multistage amplification. large volume, susceptible to external noise and high price. In this respect, the optical measurement techniques are widely proposed in these days in this concerned field ascribed to the following advantages : immune to external EMI noise and broad band response of the Pockels cell covering from DC to GHz. However, the reliability of several proposed techniques enabling to measure the electric field inside the large high power apparatus has not yet been well approved In this work, an optical measuring system, based on the Pockels effect, has been developed for measuring the field variation due to the corona discharges occurring in air and in oil. This system consists of He-Ne laser, single mode optical fiber, multi mode optical fiber, polarizing film, Y-cut LiNbO3 cell, photo detector, digital oscilloscope and personal computer with GPIB. For this purpose, optical probe has been specially designed and realized and put into the needle-plane electrode. Afterward, same measurement is carried out in oil. We demonstrate the characteristic of the optical measuring system and the measurement results.

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pH 측정 시스템의 표준화에 관한 연구 (Studies on the Standardization of pH Measurement System)

  • 이화심;김명수;김진복;오상협
    • 대한화학회지
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    • 제42권4호
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    • pp.432-442
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    • 1998
  • $pH=-loga_H$ 정의에서 pH는 단일 이온의 활동도항을 포함하므로 단지 측정의 방법만으로는 결정되어질 수 없고 근사법을 필요로 한다. 근사법에 의한 pH 측정은 수소전극과 은/염화은 전극을 사용한 액간 접촉이 없는 cell의 기전력 측정으로부터 이루어지는 방법이며, 이 과정을 통해 pH 값이 인증된 pH의 일차 표준 물질을 얻을 수 있다. 일반적인 pH 미터와 전극을 검정하는 데에는 인증된 pH 값을 가지는 표준 완충용액을 사용하므로 표준 완충용액의 pH 값 정혹도는 실제 측정된 pH 값의 실효성을 제한할 수 있다. 본 연구에서는 정확한 pH의 측정을 위해서 pH 값을 인증할 수 있는 시스템을 구축하기 위한 제반의 연구를 수행하였으며, 이를 토대로 pH 1.6∼12.5의 범위에 해당하는 완충용액의 pH를 0.005 pH 단위 이내의 불확도로 인증하였다.

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Immune Responses of NIH Mice Infected with Avirulent and Virulent Strains of Plasmodium chabaudi adami Single and Mixed Infections

  • Namazi, M.J.;Phillips, R.S.
    • Parasites, Hosts and Diseases
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    • 제48권1호
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    • pp.23-33
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    • 2010
  • An understanding of the nature of the immune response to asexual erythrocytic stages of malaria parasites will facilitate vaccine development by identifying which responses the vaccine should preferentially induce. The present study examined and compared the immune responses of NIH mice in either single or mixed infections with avirulent (DK) or virulent (DS) strains of Plasmodium chabaudi adami using the ELISA test for detecting and measurement of cytokines and antibody production. In both single and mixed infections, the study showed that both cell- and antibody-mediated responses were activated. In all experiments, an early relatively high level of IFN-$\gamma$ and IgG2a during the acute phase of the infection, and later elevation of IL-4 and IgG1, suggested that there was a sequential Th1/Th2 response. However, in the avirulent DK strain infection a stronger Th1 response was observed compared to the virulent DS strain-infection or in mixed infections. In the virulent DS infection, there was a stronger Th2 response compared to that in the DK and mixed infections. The faster proliferation rate of the virulent DS strain compared to the DK strain was also evident.

Continuous Viscosity Measurement of Non-Newtonian Fluids over a Range of Shear Rates Using a Mass-Detecting Capillary Viscometer

  • Sehyun Shin;Keum, Do-Young
    • Journal of Mechanical Science and Technology
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    • 제16권2호
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    • pp.255-261
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    • 2002
  • A newly designed mass-detecting capillary viscometer uses a novel concept to continuously measure non-Newtonian fluids viscosity over a range of shear rates. A single measurement of liquid-mass variation with time replaces the now rate and pressure drop measurements that are usually required by capillary tube viscometers. Using a load cell and a capillary, we measured change in the mass flow rate through a capillary tube with respect to the time, m(t), from which viscosity and shear rate were mathematically calculated. For aqueous polymer solutions, excellent agreement was found between the results from the mass-detecting capillary viscometer and those from a commercially available rotating viscometer. This new method overcomes the drawbacks of conventional capillary viscometers meassuring non-Newtonian fluid viscosity. First, the mass-detecting capillary viscometer can accurately and consistently measure non -Newtonian viscosity over a wide range of shear rate extending as low as 1 s$\^$-1/. Second, this design provides simplicity (i. e., ease of operation, no moving parts), and low cost.

Functional Genomics in the Context of Biocatalysis and Biodegradation

  • Koh Sung-Cheol;Kim Byung-Hyuk
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2002년도 추계학술대회
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    • pp.3-14
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    • 2002
  • Functional genomics aims at uncovering useful information carried on genome sequences and at using it to understand the mechanisms of biological function. Elucidating the unknown biological functions of new genes based upon the genomics rationales will greatly speed up the extensive understanding of biocatalysis and biodegradation in biological world including microorganisms. DNA microarrays generate a system for the simultaneous measurement of the expression level of thousands of genes in a single hybridization assay. Their data mining (transcriptome) strategy has two categories: differential gene expression and coordinated gene expression. Furthermore, measurement of proteins (proteome) generates information on how the transcribed sequences end up as functional characteristics within the cell, and quantitation of metabolites yields information on how the functional proteins act to produce energy and process substrates (metabolome). Various composite functional genomics databases containing genetic, enzymatic and metabolic information have been developed and will contribute to the understanding of the life blue print and the new discoveries and practices in biocatalysis and biodegradation that could enrich their industrial and environmental applications.

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레이저 간섭계와 포켈스 효과를 이용한 불평등 전계 측정 (A Possible Application of the Nonuniform Electric Field Measurement Using Laser Interferometer and Pockels Effect)

  • 강원종;구자윤
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제51권6호
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    • pp.240-245
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    • 2002
  • In this paper, a novel optical measuring system for the measurement of nonuniform electric field was proposed. The electric field distorted by the discharges was detected through proposed optical measuring system based on the Pockets effect and Mach-Zehnder interferometer. In order to produce distorted electric field, corona discharge was generated from needle-plane electrode in air and detected by optical measuring system. This optical measuring system is constructed by He-Ne laser, single mode optical fiber, $2{\times}2$ 50/50 beam splitter, $LiNbO_3$ Pockets cell, photo detector and PC. In this system, output signal of Pockels sensor is measured by digital oscilloscope and transferred to the PC for recording and statistical processing. Through this paper, a promising possibilities of proto-type optical measuring system were evinced.

Development of a Mushroom Powder Certified Reference Material for Element Analysis

  • Betru, Tegegn Gizachew;Yim, Yong-Hyeon;Lee, Kyoung-Seok
    • Mass Spectrometry Letters
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    • 제11권4호
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    • pp.108-112
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    • 2020
  • A certified reference material (CRM) for the analysis of nutrient elements in an edible mushroom (Ganoderma lyceum) powder has been developed (KRISS CRM 108-10-011). The mass fractions of calcium (Ca), iron (Fe), and zinc (Zn) were measured by isotope dilution inductively coupled plasma mass spectrometry (ID ICP/MS). To dissolve the fungi cell wall of mushroom consisted of chitin fibers, sample preparation method by single reaction chamber type microwave-assisted acid digestion with acid mixtures was optimized. The mean measurement results obtained from 12 sample bottles were used to assign as the certified values for the CRM and the between-bottle homogeneities were evaluated from the relative standard deviations. The certified values were metrologically traceable to the definition of the kilogram in the International System of Units (SI). This CRM is expected to be used for validation of analytical methods or quality control of measurement results in analytical laboratories when they determine the mass fractions of elements in mushroom or other similar samples.

I-V 특성곡선을 통한 태양전지 패널의 모델 파라미터 추출 방법 (Analytical Methods for the Extraction of PV panel Single-Diode model parameters from I-V Characteristic)

  • 최성원;류지형;이창구
    • 한국산학기술학회논문지
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    • 제12권2호
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    • pp.847-851
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    • 2011
  • 태양광 발전 시스템은 친환경성을 바탕으로 설치용량이 증가하고 있으며 효율 개선을 위한 연구가 활발하다. 고 효율 시스템 설계를 위해서는 태양전지 패널의 출력특성을 정확히 파악하는 것이 중요하다. 태양전지 패널은 단일 다이오드 모델로 물리적 특성을 표현할 수 있으나, 정확한 파라미터를 얻는 것은 여러 단계의 측정과 수치해석 등의 복잡한 과정을 거쳐야 한다. 본 논문에서는 패널 제조사의 데이터 시트에 제공되는 태양전지 패널의 I-V 특성곡선을 기반으로 패널의 단일 다이오드 모델의 특성 파라미터를 추출하는 방법을 제시하였다. 제시한 방법의 검증을 위하여, Simulink의 Solar Cell 블록에 추출한 파라미터를 입력하여 출력을 측정하고, 데이터 시트와 오차를 계산하였다.