• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 하계학술대회 논문집
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• pp.304-307
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• 2008

일반적으로 마이크로 전원으로 연료전지 발전장치는 100w ~수백kw의 용량을 가지며 종전의 대규모 전력설비와 비교하여 높은 신뢰도를 갖는 고품질의 전력을 공급할 수 있다. 본 연구에서는 소형 분산에너지원으로서 PEMFC(Proton Exchange Membrane Fuel Cell )연료전지를 사용하는 발전장치의 PSIM 모델을 설정하고 부하변화에 대한 연료전지 발전장치의 출력전압의 강인성을 살펴보았다. 연료전지 발전장치의 교류출력의 강인성은 선형 부하 및 비선형 부하가 급변하는 과도상태에서 파악하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권4호
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• pp.349-362
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• 2020

Temperature affects the firing pattern and electrical activity of neurons in animals, eliciting diverse responses depending on neuronal cell type. However, the mechanisms underlying such diverse responses are not well understood. In the present study, we performed in vitro recording of abdominal ganglia cells of Aplysia juliana, and analyzed their burst firing patterns. We identified atypical bursting patterns dependent on temperature that were totally different from classical bursting patterns observed in R15 neurons of A. juliana. We classified these abnormal bursting patterns into type 1 and type 2; type 1 abnormal single bursts are composed of two kinds of spikes with a long interspike interval (ISI) followed by short ISI regular firing, while type 2 abnormal single bursts are composed of complex multiplets. To investigate the mechanism underlying the temperature dependence of abnormal bursting, we employed simulations using a modified Plant model and determined that the temperature dependence of type 2 abnormal bursting is related to temperature-dependent scaling factors and activation or inactivation of potassium or sodium channels.

• The Korean Journal of Pain
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• 제35권2호
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• pp.160-172
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• 2022

Background: The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods: SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results: The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions: Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.

• Geomechanics and Engineering
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• 제34권5호
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• pp.507-527
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• 2023

Accurately estimation of the geo-mechanical parameters in Artificial Ground Freezing (AGF) is a most important scientific topic in soil improvement and geotechnical engineering. In order for this, one way is using classical and conventional constitutive models based on different theories like critical state theory, Hooke's law, and so on, which are time-consuming, costly, and troublous. The others are the application of artificial intelligence (AI) techniques to predict considered parameters and behaviors accurately. This study presents a comprehensive data-mining-based model for predicting the Young's Modulus of frozen sand under the triaxial test. For this aim, several single and hybrid models were considered including additive regression, bagging, M5-Rules, M5P, random forests (RF), support vector regression (SVR), locally weighted linear (LWL), gaussian process regression (GPR), and multi-layered perceptron neural network (MLP). In the present study, cell pressure, strain rate, temperature, time, and strain were considered as the input variables, where the Young's Modulus was recognized as target. The results showed that all selected single and hybrid predicting models have acceptable agreement with measured experimental results. Especially, hybrid Additive Regression-Gaussian Process Regression and Bagging-Gaussian Process Regression have the best accuracy based on Model performance assessment criteria.

• 조명전기설비학회논문지
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• 제20권2호
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• pp.45-49
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• 2006

본 논문은 마이크로 전극 시스템에 의하여 연료전지 및 Ni-MH 전지로의 응용을 가정한 $AB_5$계 수소저장합금인 $MmNi_{3.55}Co_{0.75}Mn_{0.4}Al_{0.3}$의 단일 입자에 대하여 전기화학적인 평가를 수행하였다. 즉 Carbon fiber 마이크로 전극을 합금 입자 한개 위에 전기적인 접촉을 이루도록 조정하고, 합금 입자 내에서 수소원자의 겉보기 화학적 확산계수를 계산하기 위하여 Potential-Step 실험을 실시하였다. 여기에서 사용되는 합금입자는 치밀하고 전도성이 있는 구형이므로 데이터 해석을 위해 구형확산 모델을 적용하였다. 실험결과로서 겉보기 확산계수($D_{app}$)는 수소 흡장 및 방출되는 전 과정에서 $10^{-9}$과 $10^{-10}[cm^2/s]$ 수준인 것으로 확인되었다. 마이크로 전극 측정 시스템에 의한 단일 입자의 전기화학적 평가는 기존의 Composite Film 전극에 비해 수소저장합금에 대해 보다 상세하고 정확한 정보를 쉽게 얻을 수 있었다.

• Journal of Veterinary Science
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• 제21권1호
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• pp.9.1-9.15
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• 2020

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

• Journal of Korean Neurosurgical Society
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• 제61권4호
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• pp.434-440
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• 2018

Objective : The purpose of this study was to find an optimal delivery route for clinical trials of intrathecal cell therapy for spinal cord injury in preclinical stage. Methods : We compared in vivo distribution of Cy5.5 fluorescent dye in the spinal cord region at various time points utilizing in vivo optical imaging techniques, which was injected into the lateral ventricle (LV) or cisterna magna (CM) of rats. Results : Although CM locates nearer to the spinal cord than the LV, significantly higher signal of Cy5.5 was detected in the thoracic and lumbar spinal cord region at all time points tested when Cy5.5 was injected into the LV. In the LV injection Cy5.5 signal in the thoracic and lumbar spinal cord was observed within 12 hours after injection, which was maintained until 72 hours after injection. In contrast, Cy5.5 signal was concentrated at the injection site in the CM injection at all time points. Conclusion : These data suggested that the LV might be suitable for preclinical injection route of therapeutics targeting the spinal cord to test their treatment efficacy and biosafety for spinal cord diseases in small animal models.

• Wind and Structures
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• 제11권3호
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• pp.221-240
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• 2008

The spanwise flow structure around a rigid smooth circular cylinder model in cross-flow has been investigated based on the experimental data obtained from a series of wind tunnel tests. Surface pressures were collected at five spanwise locations along the cylinder over a Reynolds number range of $1.14{\times}15^5$ to $5.85{\times}10^5$, which covered sub-critical, single-bubble and two-bubble regimes in the critical range. Separation angles were deduced from curve fitted to the surface pressure data. In addition, spanwise correlations and power spectra analyses were employed to study the spatial structure of flow. Results at different spanwise locations show that the transition into single-bubble and two-bubble regimes could occur at marginally different Reynolds numbers which expresses the presence of overlap regions in between the single-bubble regime and its former and later regimes. This indicates the existence of three-dimensional flow around the circular cylinder in cross-flow, which is also supported by the observed cell-like surface pressure patterns. Relatively strong spanwise correlation of the flow characteristics is observed before each transition within the critical regime, or formation of first and second separation-bubbles. It is also noted that these organized flow structures might lead to greater overall aerodynamic forces on a circular cylinder in cross-flow within the critical Reynolds number regime.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 하계학술대회 논문집 Vol.9
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• pp.429-429
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• 2008

The structural stability and the elastic modulus of hexagonal ZnO nanowires and nanotubes are investigated using atomistic simulations based on the shell model. The ZnO nanowire with (10-10) facets is energetically more stable than that with (11-20). Our calculations indicate that the structural change of ZnO nanowires with (10-10) facets is sensitive to the diameter. With decreasing the diameter of ZnO nanowires, the unit-cell length is increased while the bond-length is reduced due to the change of surface atoms. Unlike the conventional layered nanotubes, the energetic stability of single crystalline ZnO nanotubes is related to the wall thickness. The potential energy of ZnO nanotubes with fixed outer and inner diameters decreases with increasing wall thickness while the nanotubes with same wall thickness are independent of the outer and inner diameters. The transformation of single crystalline ZnO nanotubes with double layer from wurtzite phase to graphitic suggests the possibility of wall-typed ZnO nanotubes. The size-dependent Young's modulus for ZnO nanowires and nanotubes is also calculated. The diameter and the wall thickness play a significant role in the Young's modulus of single crystalline ZnO nanowires and nanotubes, respectively.

• 한국정밀공학회지
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• 제30권3호
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• pp.308-316
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• 2013

This paper proposes an observation model for a particle filter-based localization using a sonar grid map. The proposed model estimates a predicted observation by considering the properties of a sonar sensor which has a large angular uncertainty. The proposed model searches a grid which has the highest probability to reflect a sonar beam using the following procedures; (1) the reliable area of a single sonar data is determined using the footprint association model; (2) the detection probability of each grid cell in a sonar beam coverage in estimated. The proposed model was applied to the particle filter based localization, and was verified by experiments in indoor environments.