• Journal of Microbiology and Biotechnology
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• 제7권1호
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• pp.8-12
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• 1997

In order to overproduce D-xylose isomerase, the Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 5.3.1.5) gene (xylA) was fused to ${\lambda}P_{L}$ promoter. The promoterless xylA gene containing the ribosome binding site and coding region for D-xylose isomerase was cloned into a site 0.3 kb downstream from the ${\lambda}P_{L}$ promoter on a high copy number plasmid. An octameric XbaI linker containing TAG amber codon was inserted between 33rd codon of ${\lambda}N$ and the promoterless xylA gene. The resulting recombinant plasmid (designated as pPX152) was transformed into E. coli M5248 carrying a single copy of the temperature sensitive ${\lambda}cI857$ gene on its chromosomal DNA. When temperature-induced, the transformants produced 15 times as much D-xylose isomerase as that of D-xylose-induced parent strain. The amount of overproduced D-xylose isomerase was found to be about 60% of total protein in cell-free extracts.

• 한국실내디자인학회논문집
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• 제17권3호
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• pp.34-42
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• 2008

The objective of this research is not only making the planning guides regarding a small-scale residential remodeling but also examining closely the social roles of the interior design for the poor and the underprivileged class by analyzing the residential remodeling cases. The scope of research includes 40 remodeling houses free of charge which are broadcasted in the TV program 'Love House'. Taking in consideration the characteristics of users like parentless or single-parent families and families with disabled persons, this study uses the spatial approach, user's convenience approach and psychological problem solving approach, in terms of the methodology. In result of the analysis of their interior design, first of all, it is essential solving the problem of limited house space by comparison with the family number. Second, the universal design concept for the family with disabled persons(72.5%), old persons or children is strengthened guaranteeing privacy in small and old interior space. Third, the interior design is focused in increasing communication among family members, because mental and physical obstacle would obstruct accessibility among them. This research shows the importance of the interior design which makes wide social roles contributing to create "welfare society".

• Parasites, Hosts and Diseases
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• 제45권4호
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• pp.287-293
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• 2007

The identification and characterization of antigens that elicit human T cell responses is an important step toward understanding of Leishmania major infection and ultimately in the development of a vaccine. Micropreparative SDS-PAGE followed by electro transfer to a PVDF membrane and elution of proteins from the PVDF, was used to separate 2 novel proteins from L. major promastigotes, which can induce antibodies of the IgG2a isotype in mice and also are recognized by antisera of recovered human cutaneous leishmaniasis subjects. Fractionation of the crude extract of L. major revealed that all detectable proteins of interest were present within the soluble Leishmania antigens (SLA). Quantitation of these proteins showed that their expression in promastigotes is relatively very low. Considering the molecular weight, immunoreactivity, chromatographic and electrophoretic behavior in reducing and non-reducing conditions, these proteins are probably 2 isoforms of a single protein. A digest of these proteins was resolved on Tricine-SDS-PAGE and immunoreactive fragments were identified by human sera. Two immunoreactive fragments (36.4 and 34.8 kDa) were only generated by endoproteinase Glu-C treatment. These immunoreactive fragments or their parent molecules may be ideal candidates for incorporation in a cocktail vaccine against cutaneous leishmaniasis.

• Bulletin of the Korean Chemical Society
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• 제13권2호
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• pp.127-131
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• 1992

Changes in network structures of ${\alpha}-Nb_2O_5$ in the X MgO+(1-X) ${\alpha}-Nb_2O_5$ solid solutions occurring as the MgO doping level (X) was varied were investigated by means of infrared spectroscopy and X-ray analysis. X-ray diffraction revealed that all the synthesized specimens have the monoclinic structure. The FT-IR spectroscopy showed that the system investigated forms the solid solutions in which $Mg^{2+}$ ions occupy the octahedral sites in parent crystal lattice. Electrical conductivities were measured as a function of temperature from 600 to $1050{\circ}$ and $P_{O2}$ form $1{\times}10^{-5}$ to $2{\times}10^{-1}$ atm. The defect structure and conduction mechanism were deduced from the results. The $1}n$ value in ${\alpha}{\propto}{P_{O2}^{1}n}}$ is found to be -1/4 with single possible defect model. From the activation energy ($E{\alpha}$ = 1.67-1.73 eV) and the1/n value, electronic conduction mechanism is suggested with a doubly charged oxygen vacancy.

• 토지주택연구
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• 제11권4호
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• pp.1-13
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• 2020

This research explores the generational similarities and discrepancies of socio-demographic and housing statuses between young adult children leaving the parental home and their parents. Utilizing the 20th Korean Labour and Income Panel Study (KLIPS), this study identified a total of 609 households who have left their parent home from 1999 to 2017. Two-thirds of the adult children were married couples while the rest was singles. Children's educational level was higher, and their household size was smaller than their parents. Both generations were mainly headed by employed and married men. The vast majority of the adult children lived in the same area with their parents and lived as tenants in much smaller housing than their parents. On the contrary, most parents were homeowners of a large single-family home. The generational differences were clearly observed in housing tenure, housing structure, and housing size. Although leaving parents' home is part of a transition to adulthoods (depending on the stability of the labor market and the affordability in the housing market), that process was largely triggered by the employment status that can lead to economic independence rather than their marital status. Both housing and job opportunities are important factors to determine independent life.

• Nuclear Engineering and Technology
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• 제54권4호
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• pp.1374-1381
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• 2022

Under the influence of nuclear radiation, the reliability of steam generators (SGs) is an important factor in the efficiency and safety of nuclear power plant (NPP) reactors. Motion planning that remotely manipulates an SG mobile tube-inspection robot to inspect SG heat transfer tubes is the mainstream trend of NPP robot development. To achieve motion planning, conditional traversal is usually used for base position optimization, and then the A^* algorithm is used for path planning. However, the proposed approach requires considerable processing time and has a single expansion during path planning and plan paths with many turns, which decreases the working speed of the robot. Therefore, to reduce the calculation time and improve the efficiency of motion planning, modifications such as the matrix method, improved parent node, turning cost, and improved expanded node were proposed in this study. We also present a comprehensive evaluation index to evaluate the performance of the improved algorithm. We validated the efficiency of the proposed method by planning on a tube sheet with square-type tube arrays and experimenting with Model SG.

• 천문학회보
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• 제45권1호
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• pp.62.1-62.1
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• 2020

We present a scaling relation between black hole (BH) mass and bulge luminosity for 35 nearby (z<0.1) type 1 active galaxies, selected from the 70-month Swift-BAT X-ray source catalog. Thanks to the unbiased selection and proximity of the parent sample, our sample is suitable to study the physical connection between central black holes and host galaxies. We use the F814W images obtained with the Advanced Camera for Surveys on Hubble Space Telescope, to perform the imaging decomposition with GALFIT. With a careful treatment on the PSF model, we measure the I-band bulge brightness robustly. In combination with the BH mass estimated from a single-epoch spectroscopic data, we present the correlation between BH mass and bulge luminosity of the target AGNs. We demonstrate that our sample marginally lies off from the M(BH)-L(bulge) relation of inactive galaxies. We discuss possible physical origins of this discrepancy. Finally, we present how the relation depends on the photometric properties of AGNs and host galaxies, which may provide an useful insight on the co-evolution between BHs and host galaxies.

• Journal of Hospice and Palliative Care
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• 제7권1호
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• pp.64-72
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• 2004

목적: 본 연구는 3차 의료기관에서 진행암 및 말기암 환자와 가족을 대상으로 집단 교육을 실시하여 그 효과성을 보고자 하였다. 방법: 집단교육에 참석한 환자와 가족을 대상으로 설문지를 실시했다. 응답한 설문지를 SAS 분석하고 CHISQ를 통해 유의성 검증을 하였다. 설문지 조사내용은 1) 참석자의 일반적인 특성, 2) 환자의 일반적인 특성, 3) 환자를 돌보는 어려움, 4) 교육에 대한 평가였다. 결과: 교육에 참석한 환자와 가족은 211명이었고, 이 중 응답한 설문지는 89명이었다. 응답한 설문지 결과를 보만 참석자 중 환자가 37.5%, 가족이 56.2% 참석했다. 참석자의 나이는 60대, 50대, 40대, 30대 순이었고, 가족관계는 배우자가 가장 많고, 부모, 자녀, 형제 순으로 참석했다. 환자의 진단은 위암, 폐암, 유방암, 대장암 순이었고, 진행암 환자가 48.8%, 말기가 17.4%를 차지했다. 환자와 가족이 호소하는 주된 어려움은 치료와 관련된 문제가 대부분이었고, 정서적인 문제, 식사문제, 증상관리, 통증조절, 의료문제의 어려움을 호소했다. 교육에 대한 평가는 95%가 도움이 되었다고 하였다. 특히 식사, 의료문제에 대한 교육 만족도가 높았다. 주목할 점은 환자와 가족은 치료와 관련된 문제뿐만 아니라 호스피스와 관련된 구체적인 정보를 알고 싶어했다. 결론: 이 프로그램은 진행암 및 말기암 환자와 가족을 대상으로 집단 교육을 실시하여 필요한 정보를 제공함으로써 교육이 도움이 되었다는 결과를 얻었다. 향후 프로그램은 환자와 가족이 필요로 하는 구체적인 정보와 충분한 교육시간의 확보와 보다 많은 교육의 기회의 제공이 요구된다.

• 가족자원경영과 정책
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• 제16권1호
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• pp.199-227
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• 2012

Studies of leisure time generally have been concentrated on the amount of time spent by each family member in Korea. In this study, the shared leisure time among family members in various leisure activities was mainly investigated. Purposes of this study were to describe how family members spent the leisure time together and to investigate the determinants that affected whether or not family shared leisure time together. The data source was the '2009 Time Use Survey' conducted by the Korea National Statistical Office. The person involved in each activity was surveyed for the first time in the survey. The data from 17,470 diaries(10,359 weekdays and 3,549 Sunday) from 8,745 individuals aged from 20 to 59 who had a spouse were analyzed. The descriptive statistics and logistic regressions were used. On average, married couples spent leisure time together with spouses 36 minutes a day on weekdays and 1 hour and 38 minutes on Sunday, respectively. Parent did with preschool children 5 minutes a day on weekdays and 15 minutes on Sunday, respectively. The shared leisure time with other family and relatives was 22 minutes a day on weekdays and 1 hour and 13 minutes on Sunday, respectively. Although there was a wide variation on the shared family leisure time by the types of activities, the main shared leisure activity was using mass media, which accounts for about two third of all shared leisure time together with family. Independent variables, including the age, sex, education level, working hours, personal income, presence of preschool children, marital status, sex role attitude, and dual or single income earner were the significant determinants of family's or couple's parent-preschool children's shared leisure time on weekdays or Sunday. Based on the results of this study, family and leisure policy were suggested to increase active family shared leisure time.

• 부모자녀건강학회지
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• 제5권2호
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• pp.206-223
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• 2002

The purpose of this study was to discuss the nature of pregnancy experience among unwed Korean mothers and to figure out what it meant for them, by asking some of them to reflect on themselves in a written form from interpretative and phenomenological perspective, based on van Manen's research method of the same approach. The subjects in this study were seven unmarried mothers in Korea. The data were collected from July through December 2000 through in- depth interview and observation. The statements of the participants were taped, recorded, analyzed and select essential themes. To have a phenomenological understanding of the pregnancy unmarried mothers, literary works, including essays and novels, were reviewed. The findings of this study were as below: The selected substantial themes about the pregnancy and birth experiences of the single mothers included 'hatred for boy friend,' 'hatred for the fetus,' and 'hatred for myself.' The unmarried mothers met their boy friends through the introduction of friends. At first, they were good friends, but they soon went on a date and had sexual relation with no common knowledge about sex. Most of them were aware of their pregnancy after they felt fetal movement, but they put off taking the test because they didn't want to accept the fact and feared it. Such an attitude was rare among married pregnant women, and being pregnant with no prior preparation or sexual knowledge is not common in Western countries. They felt guilty and informed their boy friends of the fact, but the boy friends didn't admit their responsibility and rejected the babies. This made the poor girls hate them, and it turned into severe abhorrence. They thought their lives were ruined by the babies, and wished they would disappear. They reproached the babies, hitting their own bellies or making a kick. They considered themselves to be abandoned and didn't want to live any more. Giving themselves up to despair, some attempted to commit a suicide. They hoped to die, thinking their death would solve the problem. When they lost a chance of abortion and were about to have the babies adopted, they felt sorry for them. The unwanted pregnancy gave them a pain, but they keenly felt they were loved by their mothers, and learned the value of family. It's attempted in this study to clearly show how much unmarried Korean mothers suffered from pregnancy, and this effort paved the way for seeing unwed mothers in a new light and having a better understanding of them, instead of sticking to general perception.