• 산업진흥연구
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• 제6권2호
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• pp.55-62
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• 2021

한 부모 가족은 2000년 이후로 증가하였는데 50대의 이혼율의 급증과 40대와 50대 남성의 사망률 증가와도 관련이 있다. 한 부모 중년 여성가장은 생의 발달 과정이라는 시기와 맞물려 자기실현의 의식화 과정이 드러나는 시기에 속한다. 이러한 측면에서 한 부모 중년여성가장의 GIM을 통한 자기실현의 과정을 연구할 필요성이 있다. 이에 본 연구는 2018년 9월부터 2020년 6월까지 4명의 한 부모 중년 여성가장을 연구 참여자로 선정, 10~11회기의 GIM을 진행하여 다음과 같은 결론이 도출되었다. 첫째, 한 부모 중년 여성가장이 GIM을 통해 경험한 심상을 신체, 감정, 기억사고, 감각의 4개 하위 구성 요소로 분류하고, 8개의 의미단위 요소를 46개로 범주화하였으며, 152개의 의미단위를 도출하였다. 둘째, GIM을 통해 경험된 자기실현 과정은 참여자들에게 그림자와 페르소나, 자기라는 원형상으로 나타났다. 참여자들은 GIM 안에서 현재 가족관계의 부정적인 감정을 언어로 표현하고 수동적인 자신의 심상 안에서 자신의 생각을 적극적으로 변화시키는 경험을 통하여 그림자의 의식화 과정을 거쳤으며, 긍정적이고 밝았던 자신의 내적인 모습을 재확인하였다. 이들은 부모나 형제들에게 억압되고 소외된 자신의 삶이 착한 딸과 종교인이라는 페르소나에 억눌린 상태와, 분노하는 자신과 타인을 향한 이중적인 사고와 감정을 지니게 된 현재 상태를 연관 지어 경험하며 페르소나의 자각과 재정립을 이루었다. 결론적으로 본 연구를 통해서 다양한 심상의 경험과 음악을 접하며 부정적 사건의 재경험을 이끌어 냄으로써 고유한 자기로 새롭게 탄생하는 본질적인 자기다움의 자기실현과정을 이루는데 의의가 있다

• 사회복지연구
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• 제41권1호
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• pp.5-37
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• 2010

본 연구는 장기간의 활동을 통해 다양한 변화 과정을 경험해온 빈곤 한부모여성 자조집단을 대상으로, 정체기 이후 자조집단 참여자들이 변화를 주도하게 된 동기와 과정 및 전략을 살펴보고 그 변화가 참여자들과 소속집단, 기관에 주는 시사점을 분석함으로써 자조집단이 지향해야 할 실천방향을 모색하는데 목적이 있다. 이를 위해 참여자와 실천가가 협력적으로 대안을 모색하는 참여실행 연구방법(PAR)을 적용하여, 문제도출, 지역조사 및 계획, 실행, 평가 및 검토의 4단계에 걸친 연구과정을 통해 변화의 과정과 내용을 분석하고 그에 따른 사회복지실천의 함의를 고찰하였다. 연구결과, 첫째, 자조집단 참여자들의 변화 동기는 주체적으로 활동방향을 결정해야 한다는 문제인식에서 비롯되었으며, 그 결과 자신들의 역량을 고려한 활동모색을 변화의 필요성으로 도출하였다. 둘째, 변화를 모색하는 과정에서 참여자들은 변화의 범위와 내용을 주도적으로 결정했으며, 그 결과 정서적 지지를 넘어서 '목적성 있는 활동'을 지향하고자 하였다. 셋째, 변화를 위한 구체적인 전략으로 '의미있는 활동'이 제시되었으며 이는 지역자원봉사활동과 한부모 참여자 강사활동으로 계획·실행되었다. 결론적으로 자조집단이 지향해야 할 변화는, 참여자들의 주체적 인식의 변화를 기반으로 실천가 중심에서 참여자 중심의 실천으로 실천주체가 변화해야 할 것으로 나타났으며, 이를 통해 자신의 삶을 조성하는 사회적 조건에 대한 통제력을 갖도록 사회적 차원의 임파워먼트를 강화하는 것으로 나타났다. 나아가 자조집단의 한계를 극복하기 위해 공동체적 접근으로의 변화를 고려해보아야 할 필요성이 있는 것으로 제시되었다. 이러한 분석결과를 바탕으로 본 연구가 사회복지실천에 주는 함의를 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제27권4호
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• pp.718-724
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• 2017

The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the C-terminus of scFv57R in order to help it resist adverse stress and improve the stability and half-life. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.

• 농업과학연구
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• 제45권3호
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• pp.385-400
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• 2018

Marker-assisted backcrossing (MABC) is useful for selecting offspring with a highly recovered genetic background for a recurrent parent at early generation unlike rice and other field crops. Molecular marker sets applicable to practical MABC are scarce in vegetable crops including tomatoes. In this study, we used the National Center for Biotechnology Information- short read archive (NCBI-SRA) database that provided the whole genome sequences of 234 tomato accessions and selected 27,680 tag-single nucleotide polymorphisms (tag-SNPs) that can identify haplotypes in the tomato genome. From this SNP dataset, a total of 143 tag-SNPs that have a high polymorphism information content (PIC) value (> 0.3) and are physically evenly distributed on each chromosome were selected as a MABC marker set. This marker set was tested for its polymorphism in each pairwise cross combination constructed with 124 of the 234 tomato accessions, and a relatively high number of SNP markers polymorphic for the cross combination was observed. The reliability of the MABC SNP set was assessed by converting 18 SNPs into Luna probe-based high-resolution melting (HRM) markers and genotyping nine tomato accessions. The results show that the SNP information and HRM marker genotype matched in 98.6% of the experiment data points, indicating that our sequence analysis pipeline for SNP mining worked successfully. The tag-SNP set for the MABC developed in this study can be useful for not only a practical backcrossing program but also for cultivar identification and F1 seed purity test in tomatoes.

• 한국콘텐츠학회논문지
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• 제18권5호
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• pp.502-513
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• 2018

본 연구는 영아유기에 관련된 위기임신 및 출산 지원정책과 청소년 미혼부모 정책에 관한 대학생의 지지도를 살펴보고자 수행되었다. 2015년 5월부터 6월까지 서울경기지역의 미혼인 청년대학생에게 설문조사를 통해 자료를 수집한 서술적 조사연구가 수행되었다. 연구결과는 위기 임신과 출산 지원 및 전문적인 성교육을 통한 예방교육 실시, 현행 법령과 제도의 보완에 이르는 위기임신 및 출산정책 전반에 대학생의 지지율이 높은 것으로 나타났다. 또한 청소년 한부모 정책 전반에 관해서도 대학생들은 지지적인 태도를 보이는 것으로 나타났다. 청소년 한부모 정책 중 부모교육 및 경제적 교육, 재임신 예방을 위한 교육 정책에 관한 지지율은 다른 한부모 정책 지지율보다 높은 것으로 보고되었다. 종교유무에 따른 정책 지지도에 차이가 있었으며, 종교가 있다고 응답한 경우 그렇지 않은 경우보다 청소년 한부모 지원정책 중 다수 항목에서 지지율이 높은 것으로 나타났다. 이와 같은 연구결과를 토대로 위기임신 및 출산과 청소년 미혼부모 정책의 필요성에 관해 논의하고, 관련 정책의 개정 및 지원 서비스 구축을 위한 기초자료로 활용을 제안한다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.767-771
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• 2005

In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4${\times}$4 diallel cross, involving 4 chicken breeds, which were White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). Total of 331 differential displayed cDNA bands from 1,161 were displayed in the 4${\times}$4 diallel cross combinations with 30 pairs of primers, which shows the differences of gene expression between hybrids and their parental lines were very obvious in quantity and quality. Seven types of differential expression patterns were found: Co-dominance expressed pattern (T1), under-expression of parental fragments in hybrids (T2), over-expression of parental fragments in hybrids (T3), hybrid-absence expressed pattern (T4), single parentspecific expressed pattern (T5), dominant expression fragments of single parent in hybrids (T6), hybrid-specific expressed pattern (T7). Correlation analysis indicated that there were significant correlations between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old chickens(p<0.01), while there were negative significant correlations between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old birds (p<0.01).

• 원예과학기술지
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• 제32권4호
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• pp.535-543
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• 2014

Backcross breeding is the method most commonly used to introgress new traits into elite lines. Conventional backcross breeding requires at least 4-5 generations to recover the genomic background of the recurrent parent. Marker-assisted backcrossing (MABC) represents a new breeding approach that can substantially reduce breeding time and cost. For successful MABC, highly polymorphic markers with known positions in each chromosome are essential. Single nucleotide polymorphism (SNP) markers have many advantages over other marker systems for MABC due to their high abundance and amenability to genotyping automation. To facilitate MABC in hot pepper (Capsicum annuum), we utilized expressed sequence tags (ESTs) to develop SNP markers in this study. For SNP identification, we used Bukang $F_1$-hybrid pepper ESTs to prepare a reference sequence through de novo assembly. We performed large-scale transcriptome sequencing of eight accessions using the Illumina Genome Analyzer (IGA) IIx platform by Solexa, which generated small sequence fragments of about 90-100 bp. By aligning each contig to the reference sequence, 58,151 SNPs were identified. After filtering for polymorphism, segregation ratio, and lack of proximity to other SNPS or exon/intron boundaries, a total of 1,910 putative SNPs were chosen and positioned to a pepper linkage map. We further selected 412 SNPs evenly distributed on each chromosome and primers were designed for high throughput SNP assays and tested using a genetic diversity panel of 27 Capsicum accessions. The SNP markers clearly distinguished each accession. These results suggest that the SNP marker set developed in this study will be valuable for MABC, genetic mapping, and comparative genome analysis.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1078-1087
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• 2018

Objective: The genetic diversity of the Landrace population, a representative maternal pig breed in Korea, is important for genetic improvement. Previously, the effective population size (Ne) has been used to infer the genetic diversity of a population of interest. In this study, we aimed to use single nucleotide polymorphism (SNP) data to characterize linkage disequilibrium (LD) and the Ne of the Korean Landrace population. Methods: We genotyped 1,128 Landrace individuals from three representative Korean major grand-grand-parent (GGP) farms using the Illumina PorcineSNP60 version2 BeadChip, which covers >61,565 SNPs located across all autosomes and mitochondrial and sex chromosomes. We estimated the expected LD and current Ne, as well as ancestral Ne. Results: In the Korean Landrace population, the mean LD ($r^2$) of 3.698 million SNP pairs was $0.135{\pm}0.204$. The mean $r^2$ decreased slowly with as the distance between SNPs increased, and remained constant beyond 3 Mb. According to the $r^2$ calculations, 8,085 of 3.698 million SNP pairs were in complete LD. The current Ne (${\pm}$standard deviation) of the Korean Landrace population is approximately 92.27 [79.46; 105.07] individuals. The ancestral Ne exhibited a slow and steady decline from 186.61 to 92.27 over the past 100 generations. Additionally, we observed more a rapid Ne decrease from the past 20 to 10 generations ago, compared with other intervals. Conclusion: We have presented an overview of LD and the current and ancestral Ne values in the Korean Landrace population. The mean LD and current Ne for the Korean Landrace population confirm the genetic diversity and reflect the history of this pig population in Korea.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1223-1232
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• 2017

Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating-type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of C. militaris were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of C. militaris. After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with MAT1-1 and the others with MAT1-2. Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4-bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new C. militaris strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing C. militaris strains.

• Journal of Preventive Medicine and Public Health
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• 제42권6호
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• pp.392-402
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• 2009

Objectives : The aims of this paper were to develop the composite deprivation index (CDI) for the sub-district (Eup-Myen-Dong) levels based on the theory of social exclusion and to explore the relationship between the CDI and the standardized mortality ratio (SMR). Methods : The paper calculated the age adjusted SMR and we included five dimensions of social exclusion for CDI; unemployment, poverty, housing, labor and social network. The proxy variables of the five dimensions were the proportion of unemployed males, the percent of recipients receiving National Basic Livelihood Security Act benefits, the proportion of households under the minimum housing standard, the proportion of people with a low social class and the proportion of single-parent household. All the variables were standardized using geometric transformation and then we summed up them for a single index. The paper utilized the 2004-2006 National Death Registry data, the 2003-2006 national residents' registration data, the 2005 Population Census data and the 2005-2006 means-tested benefit recipients' data. Results : The figures were 115.6, 105.8 and 105.1 for the CDI of metropolitan areas (big cities), middle size cities and rural areas, respectively. The distributional variation of the CDI was the highest in metropolitan areas (8.9 - 353.7) and the lowest was in the rural areas (26.8 - 209.7). The extent and relative differences of deprivation increased with urbanization. Compared to the Townsend and Carstairs index, the CDI better represented the characteristics of rural deprivation. The correlation with the SMR was statistically significant and the direction of the CDI effects on the SMR was in accordance with that of the previous studies. Conclusions : The study findings indicated mortality inequalities due to the difference in the CDI. Despite the attempt to improve deprivation measures, further research is warranted for the consensus development of a deprivation index.