• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.1
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• pp.57-60
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• 1985

The inhibitory substance on the growth of mung bean seedling was amorphous crystal that had a single spot on silica gel thin layer chromatography under ultra-violet irradiation in dark room. The Rf value was 0.85 in n-butanol-acetic acid-formic acid-water(15 : 12 : 3 : 10) system, and 0.92 in n-butanol-acetic acid-water(4 : 1 : 5) system, respectively. Biochemical reaction of the inhibitor was negative to $FeCl_3$, pyrimidine, ninhydrine, folin, fehling, Salkowski and Ehrlich reaction, but the inhibitor had a blue fluorescence from irradiation of ultra-violet rays and had maximum absorption at 252nm on UV spectrum. On IR spectrum, the Peaks of the inhibitor appeared at $3300{\sim}3500,\;2900{\sim}3000,\;1600{\sim}1700,\;and\;1400cm^{(-1)}$. The strain tested was identified to be similar Streptomyces iuteogriseus by morphorogical and physiological characteristics.

• Restorative Dentistry and Endodontics
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• v.9 no.1
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• pp.15-24
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• 1983

Irradiation is frequently employed as the sole therapy for oral cancer. These irradiated patients presents peculiar and progressive dental problems. But there is only scanty informations concerning specific approaches to endodontic treatment for head and neck cancer patients who have been subjected to tumorcidal doses of radiation therapy. The purpose of the present study was to determine the effects of cobalt-60 radiation on the pulpal healing of dogs after the direct pulp capping. As the experimental animals, 10 dogs (above 7-8 months after birth) were divided into 3 groups (Control, Group I, Group II). The cobalt-60 was irradiated to the Group I and Group II each 1,009 and 1,562.5 rads as single dose. As the capping material Dycal$^{(R)}$(L.D. Caulk company) was selected. After the direct pulp capping the dogs were sacrified 1, 2, 3, 4, week interval and made the original slides cut with a thickness of 8 microns and stained with hematoxylin and eosin. After examination and comparision of all specimen, the results of this study were drawn as follows; 1. The formation of reparative dentin was observed from the 1st week in the Control group, the 2nd week in the Group I & II. The few and irregular tuble structure was appeared in the 4th week in the Control group only, but failed in the Group I & II. 2. The continuity of dentin bridge was appeared in the 3rd week in all group and the degeneration of odontoblast in the 1st week of the Group II. 3. The congestion and hemorrhage in the pulp tissue were observed in all groups until 3rd week. The inflammation was appeared within the 2nd week in the Group I and especially marked in the Group II, but absent in the Control group. 4. In cases Dycal into the pulp tissue deeply, the local necrosis of pulp and decrease of dentin formation was observed.

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.300-310
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• 1996

Recently, there have been attempts to obstruct the dentinal tubules and remove the smear layer by way of laser irradiation in the root canal during endodontic treatment. This treatment was designed to make the root canal to be nonporous. Using 33 extracted single rooted teeth, 30 teeth were divided into 3 groups (10 each), and 3 teeth were used as samples for SEM. Using Nd : YAG laser, the control group was not irradiated, experimental group l(1W group) was irradiated with 1W, 15pps, 15sec., 3 times, 6.7mJ and experimental group 2(3W group) was irradiated with 3W, 15pps, 15sec., 3 times 20mJ. Thereafter the roots were immersed in methylene blue for 8 hours, and the dye infiltration pattern was observed under stereomicroscope and canal wall surface change was observed under SEM. The results are as following ; 1. As a result of evaluating the dye infiltration rate of the apical and middle 1/3, there was significant difference between control group and 1W group, control group and 3W group and there was no significant difference between 1 W group and 3W group. 2. In each group, as a result of comparing the dye infiltration rate of the apical and middle 1/3, there was no significant difference in control and 1W group but significant difference in 3W group. 3. In the control group smear layer was scarecely found and many dentinal tubules were found to be open. 4. In the 1W group, the number of dentinal tubules were decreased and gradual changes of the dentin surface could be seen and 3W group, almost no dentinal tubules could be found and the dentin surface was changed a little more and showed signs of partial fusion.

• Archives of Plastic Surgery
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• v.38 no.6
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• pp.803-807
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• 2011

Purpose: The safety of radiation therapy after breast reconstruction using transverse rectus abdominis myocutaneous (TRAM) flap is still being debated, and few studies exist on the outcome of irradiation after immediate TRAM breast reconstruction. Some authors presented satisfactory outcomes after adjuvant radiation therapy on reconstructed breast with pedicled TRAM flap, while others reported significant post radiation changes of the flap. Effect of radiation therapy on TRAM flap was evaluated to see whether adjuvant radiation therapy was tolerable. Methods: 1000 immediate TRAM breast reconstruction was done by a single surgeon from July, 2001 to December, 2009. Among them 105 patients required adjuvant radiation therapy because of advanced disease or locoregional recurrence. Fat necrosis, radiation fibrosis, mastectomy skin flap necrosis, need for secondary touch up procedures, patient satisfaction were evaluated. Results: The incidence of fat necrosis was 10.5% and significant radiation fibrosis occurred in only one patient. Delayed wound problem did not occur during or after irradiation. Secondary touch-up procedures were performed in 12.3%, the most common being fat graft (8.6%). Average patient satisfaction score was 8.62/10, which was not significantly different from the authors' previous report involving all the TRAM patients (8.50). Conclusion: Radiation therapy did not increase the complication rate significantly. Aesthetic result was affected but was tolerated in most cases. The fear of adjuvant radiation is not a negative factor in selecting immediate breast reconstruction with TRAM flap.

• The Korean Journal of Pharmacology
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• v.24 no.2
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• pp.217-220
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• 1988

At present, the treatment of the radiation-induced diseases are only performing by the palliative treatment technique. Moreover, radioprotective drugs are a little toxic for human being and this seriously limits their application with various complication in clinical uses. Accordingly, new radioprotectors need developing. In our Lab., we synthesized trithioformaldehyde (TTFA), containing three sulfur atom, and examined the effect on mouse jejunal crypt cells after irradiation. Mice treated with TTF A (2.0 g/ kg) showed 78% survival ratio at 30 day after 800 rad irradiation. 1.0 g/kg and 2.0 g/kg of TTF A increased resistance of jejunal crypt cells to single doses of radiation by protection factors of 1.17 and 1.23, respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.15
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• pp.6263-6267
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• 2014

Background: It is standard treatment to combine chemotherapy (CT) and thoracic radiotherapy (TRT) in treating patients with limited stage small cell lung cancer (LS-SCLC). However, optimal timing of TRT is unclear. We here evaluated the survival impact of early versus late TRT in patients with LS-SCLC. Materials and Methods: Follow-up was retrospectively analyzed for seventy consecutive LS-SCLC patients who had successfully completed chemo-TRT between January 2006 and January 2012. Patients received TRT after either 1 to 2 cycles of CT (early TRT) or after 3 to 6 cycles of CT (late TRT). Survival and response rates were evaluated using the Kaplan-Meier method and comparisons were made using the multivariate Cox regression test. Results: Median follow-up was 24 (5 to 57) months. Carboplatin+etoposide was the most frequent induction CT (59%). Median overall, disease free, and metastasis free survivals in all patients were 15 (5 to 57), 5 (0 to 48) and 11 (3 to 57) months respectively. Late TRT was superior to early TRT group in terms of response rate (p=0.05). 3 year overall survival (OS) rates in late versus early TRT groups were 31% versus 17%, respectively (p=0.03). Early TRT (p=0.03), and incomplete response to TRT (p=0.004) were negative predictors of OS. Significant positive prognostic factors for distant metastasis free survival were late TRT (p=0.03), and use of PCI (p=0.01). Use of carboplatin versus cisplatin for induction CT had no significant impact on OS (p=0.634), DFS (p=0.727), and MFS (p=0.309). Conclusions: Late TRT appeared to be superior to early TRT in LS-SCLC treatment in terms of complete response, OS and DMFS. Carboplatin or cisplatin can be combined with etoposide in the induction CT owing to similar survival outcomes.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5371-5374
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• 2013

Objective: Photodynamic therapy (PDT) is an emerging therapeutic procedure suitable for the treatment of cervical cancer. However, the side effects of PDT are severe, including skin ulceration, so we designed an experiment to examine the effects of multiple low-dose photodynamic therapy of 5, 10, 15, 20-tetrakis(1-methylpyridinium-4-yl) porphyrin (Tmpyp4) on tumour growth by utilizing a model in nude mice implanted with Hela cervical cancer cells. Materials and Methods: Female BALB/c nude mice (aged 5-6 weeks, weighing 18-20 g) were used. Hela cervical cancer cells were injected subcutaneously ($1{\times}10^7cells/200{\mu}L$). Ten days after injection, the mice were divided into three groups (n=6), the A group of controls without any treatment, the B group receiving a single-treatment with Tmpyp4 (10 mg/kg, intratumor injection) and irradiation (blue laser, $108J/cm^2$), and the C group given three-treatments with Tmpyp4 (10 mg/kg, intratumor injection) and irradiation at intervals of two days. After starting treatment, tumours were measured every two days, to assess growth. At 2 weeks after the last treatment of C group, tumour tissue and organs were collected from each mouse to evaluate tumor histology and organ damage. Results: Tumour growth in C group was significantly inhibited compared with A and B groups (P<0.05), without any injury to the skin and internal organs. Conclusion: Our novel findings demonstrated that multiple low-dose photodynamic therapy of Tmpyp4 could inhibit cervical cancer growth significantly with no apparent side effects.

• Journal of the Korean Society of Clothing and Textiles
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• v.38 no.3
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• pp.415-426
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• 2014

This study investigated the color change, enhancement of dye uptake, washing fastness, and light fastness of cotton and silk when dyed with Juniperus chinensis and Terminalia chebula (combination dyeing) using various methods. The dyeing methods were 1) dyeing Juniperus chinensis extract and Terminalia chebula Retzius extract sequentially (J-T), 2) dyeing Terminalia chebula Retzius extract and Juniperus chinensis extract sequentially (T-J), 3) dyeing Juniperus chinensis extract and Terminalia chebula Retzius extract simultaneously (J+T), and 4) dyeing Juniperus chinensis extract 4 times repeatedly and followed by a dyeing Terminalia chebula Retzius extract in sequence. In terms of increased dyeing uptake, combination dyeing (dyeing 2 times like method 1, 2, 3) had the same impact as the repeated dyeing (method 4). The color of J, T combination dyeing showed wide range of mixed YR series, Y series resulting from Terminalia chebula Retzius, and R series resulting from Juniperus chinensis. In cotton, J-T dyeing method showed superior enhancement of dye uptake, and T-J method in silk. In both of the cases, Y series color from Terminalia chebula Retzius extract was mainly shown (Y series color was superior to R series color after dyeing). Therefore, it is recommended for cotton to implement T-J method and for silk to implement J-T method in order to enhance both dyeing uptake and emphasize the color of R series. The K/S value of combination dyed fabrics was decreased by increasing washing time; however, the K/S value of combination dyed fabrics was increased by increasing UV irradiation time. But after repeated washing and longer UV irradiation, ${\Delta}E$ value of combination dyed fabric became smaller compared to fabric dyed with Juniperus chinensis only. Thus, it was found that after J, T combination dyeing, fabric became less prone to fading by washing and UV than after Juniperus chinensis single dyeing was done.

• Journal of Plant Biotechnology
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• v.5 no.1
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• pp.25-31
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• 2003

As a consequence of tissue culture of rice, RAPD analysis was peformed to determine whether extended culture periods as undifferentiated calli affected the subsequent genetic constancy, and whether any resulting DNA rearrangements could be detected between sibling plants produced from the same callus. Somaclonal variation was induced at the initial stage of tissue culture and it increased with the length of culture maintenance. Of the 192 total bands, the number of polymorphic bands was 22 (11.5%), 33 (17.2%), and 49 (25.5%) in the callus of 1,3, and 6 months culture, respectively. A significantly higher level of genotypic polymorphisms between regenerants from two different somaclones was also detected, although all the regenerants were derived from a single genotype. In comparison of DNA polymorphisms between regenerants from non-irradiated and from irradiated calli, a scope of variation spectrum by gamma ray irradiation was larger than that by tissue culture. Consideration must be given to this genomic variation where attempts are to be made to use desirable somaclonal variants for plant breeding purpose and in genetic engineering program.

• Journal of Radiation Protection and Research
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• v.20 no.2
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• pp.85-95
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• 1995

Radiation measuring system using wireless communication method with single channel has been diveloped and tested. In this system, radiation signals from GM tube are transformed into digital pulses in pulse processing circuit and modulated in FSK (frequency shift keying) circuit for digital communication and then wirelessly transmitted to a receiving unit. The digital pulses received are then demodulated in FSK circuit and converted into radiation dose/dose rate in the data acquisition unit to display on the screen of a personal computer. The performance of this system was evaluated by using both a pulse generator and a standard radiation source(Cs-137). In both cases, digital pulses with 5V were observed in pulse processing circuit without distortion of their shape through wireless communication system. The experimental results of radiation measurement by this system after several test-irradiation of GM detector to a standard radiation source(Cs-137), showed good agreement with irradiation dose rate within 10% difference, and proved that this system could be effectively utillized as radiation measuring instrument. It is expected that this wireless radiation measuring system developed for the first time in Korea, can be used as a radiation monitor as well as a personal dosimeter if we can further improve this system to adopt wireless multichannel communication system.