• BMB Reports
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• 제42권3호
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• pp.160-165
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• 2009

The temperate Staphylococcus aureus phage ${\Phi}11$ harbors cI and cro repressor genes similar to those of lambdoid phages. Using extremely pure ${\Phi}11$ Cro (the product of the ${\Phi}11$ cro gene) we demonstrated that this protein possesses a single domain structure, forms dimers in solution at micromolar concentrations and maintains a largely $\alpha$-helical structure even at $45^{\circ}C$. ${\Phi}11$ Cro was sensitive to thermolysin at temperatures ranging from $55-75^{\circ}C$ and began to aggregate at ${\sim}63^{\circ}C$, suggesting that the protein is moderately thermostable. Of the three homologous 15-bp operators (O1, O2, and O3) in the ${\Phi}11$ cI-cro intergenic region, ${\Phi}11$ Cro only binds efficiently to O3, which is located upstream of the cI gene. Our comparative analyses indicate that the DNA binding capacity, secondary structure and dimerization efficiency of thermostable ${\Phi}11$ Cro are distinct from those of P22 Cro and $\lambda$ Cro, the best characterized representatives of the two structurally different Cro families.

• 한국산업정보학회논문지
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• 제16권4호
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• pp.19-26
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• 2011

중계기를 활용한 다중 입출력 기술이 차세대 광대역 무선 이동 통선의 유력한 후보로 연구되고 있다. 본 논문에서는 AF (amplify-and-forward) 방식 중계기 네트워크에서 SC-FDE (single carrier-frequency domain equalizer)를 위한 MRC (maximum ratio combining) MMSE (minimum mean-square-error) 등화 기법을 제안한다. 송신국과 수신국 간의 전송 신호와, 중계기를 통한 송신국과 수신국 간의 전송 신호를 MRC 방식으로 합쳐 MMSE 등화 기법을 적용하면 다이버시티 이득과 MMSE 등화 이득을 모두 획득하여 SC-FDE 시스템의 수신 성능을 크게 향상시킬 수 있다. AF 방식 중계기 네트워크에서 SC-FDE를 위한 MRC 계수 및 MMSE 등화탭의 수식을 정확히 도출하여 제시하고, 실험 결과를 통해 성능 향상을 확인한다.

• 한국해양공학회지
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• 제29권3호
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• pp.255-262
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• 2015

The present study developed a numerical simulation tool for the coupled dynamic analysis of multiple turbines on a single floater (or Multiple Unit Floating Offshore Wind Turbine (MUFOWT)) in the time domain, considering the multiple-turbine aero-blade-tower dynamics and control, mooring dynamics, and platform motions. The numerical tool developed in this study was designed based on and extended from the single-turbine analysis tool FAST to make it suitable for multiple turbines. For the hydrodynamic loadings of floating platform and mooring-line dynamics, the CHARM3D program developed by the authors was incorporated. Thus, the coupled dynamic behavior of a floating base with multiple turbines and mooring lines can be simulated in the time domain. To investigate the effect of asymmetric aerodynamic loading on the global performance and mooring line tensions of the MUFOWT, one turbine failure case with a fully feathered blade pitch angle was simulated and checked. The aerodynamic interference between adjacent turbines, including the wake effect, was not considered in this study to more clearly demonstrate the influence of the asymmetric aerodynamic loading on the MUFOWT. The analysis shows that the unbalanced aerodynamic loading from one turbine in MUFOWT may induce appreciable changes in the performance of the floating platform and mooring system.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1997년도 추계학술대회 논문집
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• pp.120-123
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• 1997

In this paper, we deal with the effect on magnetic properties when Nd is added to Sr ferrite bonded magnet. First, we choose SrO$_{n}$.Fe$_2$O$_3$(n=5.9), which is nonstoichiomatric composition, as specimen ferrite. Then, we add 5wt% polyvinyl alcohol and calcinate at 12$25^{\circ}C$ under $N_2$ environment for carbon coating on chemical compound specimen. After that we obtain 1.2${\mu}{\textrm}{m}$ single domain powder through grinding process for 18 hours. The single domain Sr ferrite Powder is well mixed with silage coupling and calcium stearate of 1wt% Then, it is kneaded by using polyamide12 as a binder and is pelleted. After adding Nd-Fe-B powder to the pelleted specimen, we injection-mould it under magnetic field by using anisotropic mould. Especially, when we add l3wt% Nd-Fe-B powder to the polyamide12, we obtain excellent magnetic propertiecs which are $_{B}$H$_{C}$=2.65KOe, Br=3.16KG and (BH)$_{max}$=2.61MGOeOeOeOeOe

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2017년도 추계학술대회
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• pp.611-613
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• 2017

본 논문에서는 기존의 single carrier - frequency domain equalization (SC-FDE) 전송구조에서 파일럿으로 인해 발생하는 오버헤드를 감소시키기 위한 새로운 방안을 제안한다. 기존의 SC-FDE 전송구조에서는 데이터 블록마다 파일럿을 전송하기 때문에 파일럿으로 인한 오버헤드 부담이 크다. 제안하는 SC-FDE 구조는 두 개의 파일럿 사이에 여러 개의 데이터 블록을 전송하여 선형 보간법을 통해 채널 추정 및 등화를 수행한다. 이는 기존의 구조에 비해 파일럿을 줄여서 전송하기 때문에 파일럿으로 인한 오버헤드를 감소시키면서 안정적인 채널 추정 및 등화를 가능하게 한다.

• 한국초전도ㆍ저온공학회논문지
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• 제21권3호
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• pp.27-31
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• 2019

Large grain YBCO bulk superconductors are fabricated by the top-seeded melt growth (TSMG) or top-seeded infiltration growth (TSIG) method. Both growth methods use at least one of $YBa_2Cu_3O_{7-{\delta}}$, $Y_2BaCuO_5$, $BaCuO_3$ pre-reacted precursor powders. However, the synthesis of the pre-reacted powders includes multiple calcination runs which are cost-bearing and time-consuming. In this work, we report the successful growth of single-domain YBCO bulk superconductors directly by using the powder compact that has been pressed from the mixture of $Y_2O_3$, $BaCuO_3$ and CuO powders without any intermediate grinding step. Single-domain YBCO bulk superconductor has been also prepared by using $Y_2O_3$, $BaO_2$ and CuO powders without intermediate grinding step. Investigations on the trapped magnetic field and microstructure of the melt-processed specimen show that the elimination of the repeated processes of calcinations and pulverization has hardly affected on the crystal growth and the magnetic properties of the grown YBCO bulk superconductors. However, it is thought that the presence of residual carbon affects on the size of Y211 particles in melt-processed YBCO bulk superconductor.

• 한국지진공학회논문집
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• 제25권1호
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• pp.43-52
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• 2021

According to several seismic design standards, a ground motion time history should be selected similar to the design response spectrum, or a ground motion time history should be modified by matching procedure to the design response spectrum through the time-domain method. For the response spectrum matching procedure, appropriate seed ground motions need to be selected to maintain recorded earthquake accelerogram characteristics. However, there are no specific criteria for selecting the seed ground motions for applying this methodology. In this study, the characteristics of ground motion time histories between seed motions and spectral matched motions were compared. Intensity measures used in the design were compared, and their change by spectral matching procedure was quantified. In addition, the seed ground motion sets were determined according to the response spectrum shape, and these sets analyzed the response of nonlinear and equivalent linear single degrees of freedom systems to present the seed motion selection conditions for spectral matching. As a result, several considerations for applying the time domain spectral matching method were presented.

• Current Optics and Photonics
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• 제4권6호
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• pp.509-515
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• 2020

A practical single photon source for fiber-based quantum information processing is still lacking. As a possible 1.55-㎛ quantum-dot single photon source, an InGaAsP/InP-air-aperture micropillar cavity is investigated in terms of fabrication tolerance. By properly modeling the processing uncertainty in layer thickness, layer diameter, surface roughness and the cavity shape distortion, the fabrication imperfection effects on the cavity quality are simulated using a finite-difference time-domain method. It turns out that, the cavity quality is not significantly changing with the processing precision, indicating the robustness against the imperfection of the fabrication processing. Under thickness error of ±2 nm, diameter uncertainty of ±2%, surface roughness of ±2.5 nm, and sidewall inclination of 0.5°, which are all readily available in current material and device fabrication techniques, the cavity quality remains good enough to form highly efficient and coherent 1.55-㎛ single photon sources. It is thus implied that a quantum dot contained InGaAsP/InP-air-aperture micropillar cavity is prospectively a practical candidate for single photon sources applied in a fiber-based quantum information network.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1186-1190
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• 2008

Single-chain Fv (scFv) antibody against c-Met is expected to be employed in clinical treatment or imaging of cancer cells owing to the important biological roles of c-Met in the proliferation of malignancies. Here, we show that the productivity of scFv against c-Met in Escherichia coli is significantly influenced by the orientation of its variable domains. We generated anti-c-Met scFv antibodies with two different domain orders (i.e., $V_L$-linker-$V_H$ and $V_H$-linker-$V_L$), expressed them in the cytoplasm of E. coli trx/gor deleted mutant, and compared their specific activities as well as their productivities. Productivity of total and functional anti-c-Met scFv with $V_H/V_L$ orientation was more than five times higher than that with $V_L/V_H$ format. Coexpression of DsbC enhanced the yield of soluble amounts of anti-c-Met scFv protein for both constructs. The purified scFv antibodies of the two different formats exhibited almost the same antigen-binding activities. We also compared the productivities and specific activities of anti-c-Met diabodies with $V_H/V_L$ or $V_L/V_H$ formats and obtained similar results to the case of scFv antibodies.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.529-535
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• 2011

Single-chain variable fragment (scFv) is a fusion protein of the variable regions of the heavy (VH) and light (VL) chains of immunoglobulin, connected with a short linker peptide of 10 to about 20 amino acids. In this study, the scFv of a monoclonal antibody against the third domain of human CD4 was cloned from OKT4 hybridoma cells using the phage display technique and produced in E. coli. The expression, production, and purification of anti-CD4 scFv were tested using SDS-PAGE and Western blot, and the specificity of anti-CD4 scFv was examined using ELISA. A 31 kDa recombinant anti-CD4 scFv was expressed and produced in bacteria, which was confirmed by SDS-PAGE and Western blot assays. Sequence analysis proved the ScFv structure of the construct. It was able to bind to CD4 in quality ELISA assay. The canonical structure of anti-CD4 scFv antibody was obtained using the SWISS_MODEL bioinformatics tool for comparing with the scFv general structure. To the best of our knowledge, this is the first report for generating scFv against human CD4 antigen. Engineered anti-CD4 scFv could be used in immunological studies, including fluorochrome conjugation, bispecific antibody production, bifunctional protein synthesis, and other genetic engineering manipulations. Since the binding site of our product is domain 3 (D3) of the CD4 molecule and different from the CD4 immunological main domain, including D1 and D2, further studies are needed to evaluate the anti-CD4 scFv potential for diagnostic and therapeutic applications.