• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1120-1126
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• 2013

The syndecan family consists of four transmembrane heparan sulfate proteoglycans present in most cell types and each syndecan shares a common structure containing a heparan sulfate modified extracellular domain, a single transmembrane domain and a C-terminal cytoplasmic domain. To get a better understanding of the mechanism and function of syndecan-4 which is one of the syndecan family, it is crucial to investigate its three-dimensional structure. Unfortunately, it is difficult to prepare the peptide because it is membrane-bound protein that transverses the lipid bilayer of the cell membrane. Here, we optimize the expression, purification, and characterization of transmembrane, cytoplasmic and short extracellular domains of syndecan4 (syndecan-4 eTC). Syndecan-4 eTC was successfully obtained with high purity and yield from the M9 medium. The structural information of syndecan-4 eTC was investigated by MALDI-TOF mass (MS) spectrometry, circular dichroism (CD) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. It was confirmed that syndecan-4 eTC had an ${\alpha}$-helical multimeric structure like transmembrane domain of syndecan-4 (syndecan-4 TM) in membrane environments.

• Molecules and Cells
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• 제21권1호
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• pp.1-6
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• 2006

Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) is a mitochondrial pro-apoptotic protein that has a single Bcl-2 homology 3 (BH3) domain and a COOH-terminal transmembrane (TM) domain. Although it belongs to the Bcl-2 family and can heterodimerize with Bcl-2, its pro-apoptotic activity is distinct from those of other members of the Bcl-2 family. For example, cell death mediated by BNIP3 is independent of caspases and shows several characteristics of necrosis. Furthermore, the TM domain, but not the BH3 domain, is required for dimerization, mitochondrial targeting and pro-apoptotic activity. BNIP3 plays an important role in hypoxia-induced death of normal and malignant cells. Its expression is markedly increased in the hypoxic regions of some solid tumors and appears to be regulated by hypoxia-inducible factor (HIF), which binds to a site on the BNIP3 promoter. Silencing, followed by methylation, of the BNIP3 gene occurs in a significant proportion of cancer cases, especially in pancreatic cancers. BNIP3 also has a role in the death of cardiac myocytes in ischemia. Further studies of BNIP3 should provide insight into hypoxic cell death and may contribute to improved treatment of cancers and cardiovascular diseases.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권12호
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• pp.4725-4747
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• 2020

Software defined networking (SDN) is considered to be one of the most promising paradigms in the future. To solve the scalability and performance problem that a single and centralized controller suffers from, the distributed multi-controller architecture is adopted, thus forms multi-domain SDN. In a multi-domain SDN network, it is of great importance to ensure a reliable control plane. In this paper, we focus on the reliability problem of multi-domain SDN against controller failure from perspectives of backup controller deployment and controller replication. We firstly propose a placement algorithm for backup controllers, which considers both the reliability and the cost factors. Then a controller replication mechanism based on shared data storage is proposed to solve the inconsistency between the active and standby controllers. We also propose a shared data storage layout method that considers both reliability and performance. Besides, a fault recovery and repair process is designed based on the controller backup and shared data storage mechanism. Simulations show that our approach can recover and repair controller failure. Evaluation results also show that the proposed backup controller placement approach is more effective than other methods.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권12호
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• pp.4024-4041
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• 2022

Robust reversible watermarking has attracted widespread attention in the field of information hiding in recent years. It should not only have robustness against attacks in transmission but also meet the reversibility of distortion-free transmission. According to our best knowledge, the most recent robust reversible watermarking methods adopt a single image as the carrier, which might lead to low efficiency in terms of carrier utilization. To address the issue, a novel dual-image robust reversible watermarking framework is proposed in this paper to effectively utilize the correlation between both carriers (namely dual images) and thus improve the efficiency of carrier utilization. In the dual-image robust reversible watermarking framework, a two-layer robust watermarking mechanism is designed to further improve the algorithm performances, i.e., embedding capacity and robustness. In addition, an optimization model is built to determine the parameters. Finally, the proposed framework is applied in different domains (namely domain-independent), i.e., Slantlet Transform and Singular Value Decomposition domain, and Zernike moments, respectively to demonstrate its effectiveness and generality. Experimental results demonstrate the superiority of the proposed dual-image robust reversible watermarking framework.

• 한국항공우주학회지
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• 제42권11호
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• pp.937-946
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• 2014

본 논문에서는 단일 틸트윙 무인기의 시스템 식별기법과 결과를 제시하였다. 주파수 영역의 시스템 식별 기법인 Modified Equation Error Method(MEEM)와 시간 영역의 시스템 식별 기법인 확장 칼만 필터(Extended Kalman Filter, EKF)를 사용하였다. 시스템 식별 기법의 검증을 위해 단일 틸트윙 방식 무인기인 CNUX-3의 모델을 통해 획득한 수직모드의 선형 시뮬레이션 결과에 센서 특성을 반영한 노이즈를 합성하여 가상의 비행데이터를 생성했다. 설계 변수의 변화에 따른 MEEM의 시스템 식별 성능 변화를 분석하였다. 또한 고정밀 푸리에 변환(High accuracy Fourier Transform)을 MEEM에 적용하여 정확도를 향상시켰다. MEEM과 EKF의 시스템 식별 결과를 비교하고, 최상의 성능지수를 갖는 설계 변수값과 초기값을 최적화를 통해 결정하였다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2007년도 The 1st International Symposium on Advanced Magnetic Materials
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• pp.224.1-224.1
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• 2007

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2007년도 The 1st International Symposium on Advanced Magnetic Materials
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• pp.214.2-214.2
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• 2007

• 한국광학회:학술대회논문집
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• 한국광학회 2009년도 동계학술발표회 논문집
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• pp.171-172
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• 2009

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 2006년도 High Temperature Superconductivity Vol.XVI
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• pp.16-16
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• 2006

• 산경연구논집
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• 제11권8호
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• pp.39-49
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• 2020

Purpose: Current study aimed at exploring the roles of system justification in the effects of consumers' self-identification with the threatened social in-group on the within-domain versus across-domain consumption. It focused on whether there are positive effects of both of the self-definition and the self-investment on the in-group system justification, and also explored whether the system justification, in turn, could make positive effects on the consumption. Research design, data and methodology: The self-identification was approached in view of self-definition and self-investment when the in-group was threatened by members of their out group. The empirical study was performed with the single factor within-subject design based on the feeling of the consumers' being threatened when the in-group was criticized by the others. The in-group threatened was accessed from the memory of each of the undergraduate students participating in the empirical study by asking them to remember the events by which their important in-group was perceived to be threatened in their past life. Questionnaire data collected from the undergraduate students were used to verify research hypotheses by structural equation model in Amos 21.0 program. Results: First, the self-definition positively affected the within-domain versus across-domain consumption, but did not affect the in-group system justification. Second, the self-investment positively affected the in-group system justification. Third, the system justification made positive effects on the within-domain versus the across-domain consumption. Therefore, this article could contribute to the development of the theory related to compensatory consumption in the view that there could be the positive mediation roles of system justification in the effects of consumers' self-investment to their in-group on the within-domain versus across-domain consumption when the in-group is threatened. Conclusions: The results of this study could give managerial implications to brand or product marketing managers. How to vitalize consumers' self-definition with, and self-investment to, the threatened in-group is at issue to the marketers when consumers' important in-group was threatened by others. By evoking the in-group-based self-investment to consumers when the in-group was threatened, the marketers should increase the level of the system justification, and the marketers should promote the consumers to recognize that their products or brands are included into the within-self domain.