• 생명과학회지
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• 제13권4호
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• pp.463-472
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• 2003

제5인자와 지질막 phosphatidylserine과의 상호작용은 prothrombinase 복합체의 활성을 조절하는데 중요하다. 본 연구에서 제5인자의 지질 결합부위에 위치한 Trp2063과 Trp2064를 동시에 돌연변이 시킨 재조합 제5인자를 과발현 시키고 정제하였다. 돌연변이된 제5인자는 1-10%의 phosphatidylserine을 포함하는 지질막에서 아주낮은 활성을 보였다. surface plasmon resonance에 의해서 지질막과의 결합을 측정한 결과 돌연변이된 제5인자가 본래의 제5인자보다 고정된 지질막에의 결합이 현저하게 떨어지는 것을 관찰하였다. 제5인자가 phosphatidylserine을 포함하는 지질막에 높은 친화력으로 결합하기 위해서는 Trp2063과 Trp2064가 필수적이고 이러한 상호작용은 생리적인 phosphatidylserine 농도를 포함하는 지질막 위에서 prothrombinase 복합체의 형성에 필요하다는 결론을 내렸다.

• 방사선산업학회지
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• 제2권3호
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• pp.97-104
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which leads a variety of secondary metabolites to participate in differentiation and protection of plant against environmental stresses. In this study, we isolated a full-length cDNA of the C4H gene from a black raspberry (Rubus occidentalis L.), using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of the RocC4H gene contained a 1,515 bp open reading frame (ORF) encoding a 504 amino acid protein with a calculated molecular weight of about 57.9 kDa and an isoelectric point (pI) value of 9.1. The genomic DNA analysis revealed that RocC4H gene had three exons and two introns. By multiple sequence alignment, RocC4H protein was highly homologous with other plant C4Hs, and the cytochrome P450-featured motifs, such as the heme-binding domain, the T-containing binding pocket motif (AAIETT), the ERR triad, and the tetrapeptide (PPGP) hinge motif, were highly conserved. Southern blot analysis revealed that RocC4H is a single copy gene in R. occidentalis.

• 한국음향학회지
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• 제38권5호
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• pp.568-573
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• 2019

폐질환 진단에서 청진은 다른 진단 방식에 비해 단순하고, 폐음을 이용하여 폐질환 환자식별뿐 아니라 폐음과 관련된 질병을 예측할 수 있다. 따라서 본 논문에서는 다양한 합성곱 신경방 방식을 기반으로 폐음을 이용하여 폐질환 환자를 식별하고, 소리특성에 따른 폐음을 분류하여 각 신경망 방식의 분류 성능을 비교한다. 먼저 폐질환 소견을 갖는 흉부 영역에서 단채널 폐음 녹음기기를 이용하여 폐음 데이터를 수집하고, 수집된 시간축 신호를 스펙트럼 형태의 특징값으로 추출하여 각 분류 신경망 방식에 적용한다. 폐 사운드 분류 방식으로는 일반적인 합성곱 신경망, 병렬 구조, 잔류학습이 적용된 구조의 합성곱 신경망을 사용하고 실험을 통해 각 신경망 모델의 폐음 분류 성능을 비교한다.

• Smart Structures and Systems
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• 제18권3호
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• pp.405-423
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• 2016

In this study, the feasibility of using telecommunication single-mode optical fiber (SMF) as a distributed fiber optic strain and crack sensor was evaluated in concrete pavement monitoring. Tensile tests on various sensors indicated that the $SMF-28e^+$ fiber revealed linear elastic behavior to rupture at approximately 26 N load and 2.6% strain. Six full-scale concrete panels were prepared and tested under truck and three-point loads to quantify the performance of sensors with pulse pre-pump Brillouin optical time domain analysis (PPP-BOTDA). The sensors were protected by precast mortar from brutal action during concrete casting. Once air-cured for 2 hours after initial setting, half a mortar cylinder of 12 mm in diameter ensured that the protected sensors remained functional during and after concrete casting. The strains measured from PPP-BOTDA with a sensitivity coefficient of $5.43{\times}10^{-5}GHz/{\mu}{\varepsilon}$ were validated locally by commercial fiber Bragg grating (FBG) sensors. Unlike the point FBG sensors, the distributed PPP-BOTDA sensors can be utilized to effectively locate multiple cracks. Depending on their layout, the distributed sensors can provide one- or two-dimensional strain fields in pavement panels. The width of both micro and major cracks can be linearly related to the peak strain directly measured with the distributed fiber optic sensor.

• Smart Structures and Systems
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• 제7권4호
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• pp.241-262
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• 2011

Cast iron pipe has been used as a water distribution technology in North America since the early nineteenth century. The first cast iron pipes were made of grey cast iron which was succeeded by ductile iron as a pipe material in the 1940s. These different iron alloys have significantly different microstructures which give rise to distinct mechanical properties. Insight into the non-destructive structural condition assessment of aging pipes can be advantageous in developing mitigation strategies for pipe failures. This paper examines the relationship between the small-strain and large-strain properties of exhumed cast iron water pipes. Nondestructive and destructive testing programs were performed on eight pipes varying in age from 40 to 130 years. The experimental program included microstructure evaluation and ultrasonic, tensile, and flexural testing. New applications of frequency domain analysis techniques including Fourier and wavelet transforms of ultrasonic pulse velocity measurements are presented. A low correlation between wave propagation and large-strain measurements was observed. However, the wave velocities were consistently different between ductile and grey cast iron pipes (14% to 18% difference); the ductile iron pipes showed the smaller variation in wave velocities. Thus, the variation of elastic properties for ductile iron was not enough to define a linear correlation because all the measurements were practically concentrated in single cluster of points. The cross-sectional areas of the specimens tested varied as a result of minor manufacturing defects and levels of corrosion. These variations affect the large strain testing results; but, surface defects have limited effect on wave velocities and may also contribute to the low correlations observed. Lamb waves are typically not considered in the evaluation of ultrasonic pulse velocity. However, Lamb waves were found to contribute significantly to the frequency content of the ultrasonic signals possibly resulting in the poor correlations observed. Therefore, correlations between wave velocities and large strain properties obtained using specimens manufactured in the laboratory must be used with caution in the condition assessment of aged water pipes especially for grey cast iron pipes.

• 지구물리와물리탐사
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• 제3권4호
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• pp.119-124
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• 2000

본 연구에서는 단일주파수 일방향파동방정식을 이용하여 주파수영역에서 얻어진 파동장을 델타함수(delta function)화하고 로그(logarithm)변환을 하여 정확하고 계산시간이 적은 새로운 주시계산 방법을 개발하였다. 주시 오차를 최소화하기 위하여 여러 가지 실험을 통해 격자간격과 주파수간의 경험식을 구하였다. 아이코날 방정식(eikonal equation)에 의해 얻어진 주시곡선이나 유한차분 모델링을 이용해서 얻은 스냅샷(snapshot)의 파면 등, 다른 주시 계산 방법들에 의한 결과가 본 연구에서 얻은 결과와 아주 유사하였다. 위 두 가지 방법과 달리 본 알고리즘은 투과파의 주시만을 계산한다. 본 연구에 의해서 구한 주시를 이용하여 구조보정을 해 본 결과, 원 모델과 아주 유사한 좋은 단면을 얻을 수 있었다. 본 연구의 결과를 볼 때 이 새로운 주시계산 알고리즘은 지하구조보정과 투과 토모그래피 (transmission tomography)에 필요한 직접파의 주시를 더 빨리 계산하는 방법이다.

• Journal of the Optical Society of Korea
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• 제18권3호
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• pp.261-273
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• 2014

In this paper, metal insulator metal (MIM) plasmonic slot cavity narrow band-pass filters (NBPFs) are studied. The metal and dielectric of the structures are silver (Ag) and air, respectively. To improve the quality factor and attenuation range, two novel NBPFs based on tapered structures and double cavity systems are proposed and numerically analyzed by using the two-dimensional (2-D) finite difference time domain (FDTD) method. The impact of different parameters on the transmission spectrum is scrutinized. We have shown that increasing the cavities' lengths increases the resonance wavelength in a linear relationship, and also increases the quality factor, and simultaneously the attenuation of the wave transmitted through the cavities. Furthermore, increasing the slope of tapers of the input and output waveguides decreases attenuation of the wave transmitted through the waveguide, but simultaneously decreases the quality factor, hence there should be a trade-off between loss and quality factor. However, the idea of adding tapers to the waveguides' discontinuities of the simple structure helps us to improve the device total performance, such as quality factor for the single cavity and attenuation range for the double cavity. According to the proposed NBPFs, two, three, and four-port power splitters functioning at 1320 nm and novel ultra-compact two-wavelength and triple-wavelength demultiplexers in the range of 1300-1550 nm are proposed and the impacts of different parameters on their performances are numerically investigated. The idea of using tapered waveguides at the structure discontinuities facilitates the design of ultra-compact demultiplexers and splitters.

• 방사성폐기물학회지
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• 제17권2호
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• pp.227-234
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• 2019

국내 고준위 방사성폐기물 심층처분시스템에 대한 프로세스 기반의 종합성능평가체계(APro) 개발을 위하여 사용자 편의성이 향상된 모델링 인터페이스를 구축하였다. APro의 모델링 인터페이스는 프로그래밍 언어인 MATLAB을 이용하여 구축되었고, 다중물리현상 모사가 가능한 COMSOL과 지화학반응 계산이 가능한 PHREEQC를 계산 엔진으로 활용하여 연산자분리 방식을 적용하였다. APro는 모델링 영역을 기존의 정형화된 처분시스템으로 제한함으로써 모델의 자유도는 낮지만, 사용자 편의성을 향상시켰다. 처분시스템에서 고려되는 주요 현상들을 모듈화하였고, 이를 "Default process"와 다수의 "Alternative process"로 구분하여 사용자가 선택할 수 있도록 함으로써 모델의 유연성을 높였다. APro는 크게 입력자료 부분과 계산실행 부분으로 구성된다. 기본 입력자료는 하나의 EXCEL 파일에 일정한 포맷으로 정리되고, 계산실행 부분은 MATLAB을 이용하여 코딩되었다. 최종적인 전체 계산 결과는 독립적인 COMSOL 파일 형태로 생성되도록 하여 COMSOL을 이용한 계산 결과의 후처리가 가능하도록 하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권8호
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• pp.4191-4211
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• 2019

Software product lines (SPLs) are complex software systems by nature due to their common reference architecture and interdependencies. Therefore, any form of evolution can lead to a more complex situation than a single system. On the other hand, software product lines are developed keeping long-term perspectives in mind, which are expected to have a considerable lifespan and a long-term investment. SPL development organizations need to consider software evolution in a systematic way due to their complexity and size. Addressing new user requirements over time is one of the most crucial factors in the successful implementation SPL. Thus, the addition of new requirements or the rapid context change is common in SPL products. To cope with rapid change several researchers have discussed the evolution of software product lines. However, for the evolution of an SPL, the literature did not present a systematic process that would define activities in such a way that would lead to the rapid evolution of software. Our study aims to provide a requirements-driven process that speeds up the requirements engineering process using social network sites in order to achieve rapid software evolution. We used classification, topic modeling, and sentiment extraction to elicit user requirements. Lastly, we conducted a case study on the smartwatch domain to validate our proposed approach. Our results show that users' opinions can contain useful information which can be used by software SPL organizations to evolve their products. Furthermore, our investigation results demonstrate that machine learning algorithms have the capacity to identify relevant information automatically.

• The Plant Pathology Journal
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• 제35권5호
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• pp.538-542
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• 2019

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.