• Earthquakes and Structures
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• 제21권6호
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• pp.627-639
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• 2021

Fragility curves are being more significant as a useful tool for evaluating the relationship between the earthquake intensity measure and the effects of the engineering demand parameter on the buildings. In this paper, the effect of different site conditions on the vulnerability of the structures was examined through the fragility curves taking into account different strength capacities of the precast columns. Thus, typical existing single-story precast RC industrial buildings which were built in Turkey after the year 2000 were examined. The fragility curves for the three typical existing industrial structures were derived from an analytical approach by performing non-linear dynamic analyses considering three different soil conditions. The Park and Ang damage index was used in order to determine the damage level of the members. The spectral acceleration (Sa) was used as the ground motion parameter in the fragility curves. The results indicate that the fragility curves were derived for the structures vary depending on the site conditions. The damage probability of exceedance values increased from stiff site to soft site for any Sa value. This difference increases in long period in examined buildings. In addition, earthquake demand values were calculated by considering the buildings and site conditions, and the effect of the site class on the building damage was evaluated by considering the Mean Damage Ratio parameter (MDR). Achieving fragility curves and MDR curves as a function of spectral acceleration enables a quick and practical risk assessment in existing buildings.

• Earthquakes and Structures
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• 제19권1호
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• pp.29-44
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• 2020

The effectiveness and the sensitivity of a Wireless impedance/Admittance Monitoring System (WiAMS) for the prompt damage diagnosis of two single-storey single-span Reinforced Concrete (RC) frames under cyclic loading is experimentally investigated. The geometrical and the reinforcement characteristics of the RC structural members of the frames represent typical old RC frame structure without consideration of seismic design criteria. The columns of the frames are vulnerable to shear failure under lateral load due to their low height-to-depth ratio and insufficient transverse reinforcement. The proposed Structural Health Monitoring (SHM) system comprises of specially manufactured autonomous portable devices that acquire the in-situ voltage frequency responses of a network of twenty piezoelectric transducers mounted to the RC frames. Measurements of external and internal small-sized piezoelectric patches are utilized for damage localization and assessment at various and increased damage levels as the magnitude of the imposed lateral cycle deformations increases. A bare RC frame and a strengthened one using a pair of steel crossed tension-ties (X-bracing) have been tested in order to check the sensitivity of the developed WiAMS in different structural conditions since crack propagation, damage locations and failure mode of the examined frames vary. Indeed, the imposed loading caused brittle shear failure to the column of the bare frame and the formation of plastic hinges at the beam ends of the X-braced frame. Test results highlighted the ability of the proposed SHM to identify incipient damages due to concrete cracking and steel yielding since promising early indication of the forthcoming critical failures before any visible sign has been obtained.

• The Korean Journal of Physiology and Pharmacology
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• 제8권2호
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• pp.95-100
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• 2004

Ischemic preconditioning (IPC) has been accepted as a heart protection phenomenon against ischemia and reperfusion (I/R) injury. The activation of ATP-sensitive potassium $(K_{ATP})$ channels and the release of myocardial nitric oxide (NO) induced by IPC were demonstrated as the triggers or mediators of IPC. A common action mechanism of NO is a direct or indirect increase in tissue cGMP content. Furthermore, cGMP has also been shown to contribute cardiac protective effect to reduce heart I/R-induced infarction. The present investigation tested the hypothesis that $K_{ATP}$ channels attenuate DNA strand breaks and oxidative damage in an in vitro model of I/R utilizing rat ventricular myocytes. We estimated DNA strand breaks and oxidative damage by mean of single cell gel electrophoresis with endonuclease III cutting sites (comet assay). In the I/R model, the level of DNA damage increased massively. Preconditioning with a single 5-min anoxia, diazoxide $(100\;{\mu}M)$, SNAP $(300\;{\mu}M)$ and 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP) $(100\;{\mu}M)$ followed by 15 min reoxygenation reduced DNA damage level against subsequent 30 min anoxia and 60 min reoxygenation. These protective effects were blocked by the concomitant presence of glibenclamide $(50\;{\mu}M)$, 5-hydroxydecanoate (5-HD) $(100\;{\mu}M)$ and 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate, Rp-isomer (Rp-8-pCPT-cGMP) $(100\;{\mu}M)$. These results suggest that NO-cGMP-protein kinase G (PKG) pathway contributes to cardioprotective effect of $K_{ATP}$ channels in rat ventricular myocytes.

• 한국응급구조학회지
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• 제10권1호
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• pp.51-60
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• 2006

Background: The toxicity of paraquat has been known to be caused by oxygen free radicals which leads to the lipid peroxidation and multiple organ failure. High dose vitamin C has been known to be a effective antioxidant activities against the paraquat intoxication. This study was designed to evaluate the effect of regular injection of high dose vitamin C on liver damage in paraquat poisoned rat model. Method: Fifty five rats of Sprague-Dawley strain were divided into three groups: control group, only intraperitoneally injected saline; paraquat group, single dose intraperitoneally injected paraquat(24.5%, 40 mg/kg) and every 8 hours injected saline; paraquat and vitamin C group, single dose intraperitoneally injected paraquat(24.5%, 40 mg/kg) and every 8 hours injected vitamin C(72 mg/kg). Rats were sacrificed on the 12 hours, 1st day, 2nd day, 4th day, 7th day after injection and liver tissue was obtained. H&E(Hematoxylin & Eosin) stain and Masson's trichrome stain for collagen fiber detection were undertaken. The results were observed using the microscope. Results: 1. There were no differences between control and experimental group at the 12hours after regular injection of high dose vitamin C. 2. There were significantly decreased liver damage in experimental group in the 1st day after regular injection of high dose vitamin C. 3. There were significantly increased recovery of liver damage with time in experimental group after regular injection of high dose vitamin C. Conclusion: These results suggest that regular injection of high dose vitamin C is effective in decreasing liver damage in paraquat intoxication.

• 약학회지
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• 제52권5호
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• pp.376-383
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• 2008

Chungkookjang (CKJ) is a fermented soybean product and one of favorite traditional foods in Korea. In this study, the alcoholic extract from Korean fermented soybean (CKJ) and its one of major flavonoids, genistein were evaluated for their protective effect against B(a)P induced cytotoxicity and DNA damage in HepG2 cells. CKJ extract and genistein decreased B(a)P-induced cell cytotoxicity. CKJ extract inhibited DNA single strand breaks evaluated by single cell gel electrophoresis. From RT-PCR study, it was revealed that CKJ extract decrease DNA damage induced in HepG2 cells expressing CYP1A1 and 1A2 by B(a)P. The metabolizing activities of CYP1A1 and CYP1A2, as measured by the 7-alkoxy resorufin O-deethylation (AROD) assay, showed that CKJ extract and genistein inhibited CYP1A1 and CYP1A2 activities. Genistein may contribute to these biological effects of CKJ extract at least in part. All these results indicate that CKJ extract and genistein may be useful for protection against B(a)P-induced cytotoxicity and DNA damage. Therefore, the alcoholic extract of Korean fermented soybean (CKJ) is suggested to be promising functional food which can prevent the cellular genotoxicity of dietary and lifestyle related carcinogens.

• Biomolecules & Therapeutics
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• 제13권3호
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• pp.150-155
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• 2005

The ethanolic extract of chestnut (Castanea crenata S. et Z., Fagaceae) inner shell (CISE) and one of its components, ellagic acid (EA), were evaluated for their protective effects against 1, 1-diphenyl-2-picryl hydrazine (DPPH) free radical generation and hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line. CISE and EA were shown to possess the free radical scavenging effect against DPPH radical generation, significantly. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from Chinese hamster lung (CHL) cell, assessed by single cell gel electrophoresis assay and 8-hydroxy -2'-deoxy guanosine (8-OH-2'dG) assay. Furthermore, topical application of CISE [$12.5\%$(w/w) cream] and ellagic acid [$1.0\%$(w/w) cream] for 14 days potently inhibited malondialdehyde (MDA) formation of mouse dorsal skin (a marker of lipid peroxidation) induced by ultraviolet B exposure. Therefore, CISE and its component, ellagic acid, may be the useful natural antioxidants by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage when topically applied.

• 한국항공우주학회지
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• 제38권2호
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• pp.111-118
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• 2010

복합재료 구조물에서 조인트 부위는 매우 취약한 부분이다. 본 논문에서는 후판 알루미늄-알루미늄 조인트 및 복합재-알루미늄 조인트를 접착제로 접합하여 제작한 다음, 인장실험을 수행하여 파손형태를 고찰하였다. 또한, 항복 변형률에 기초한 수정 파손영역 이론을 제안하였으며, 파괴모드별 파손하중과 상호 비교하였다. 후판 알루미늄-알루미늄 조인트와 복합재-알루미늄 조인트의 파손강도를 동일한 파손기준값을 적용하여 예측하였으며, 제작된 14종류의 시편에서는 최대 19.3% 오차범위 내에서 파손강도를 예측할 수 있었다.

• Earthquakes and Structures
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• 제2권1호
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• pp.65-88
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• 2011

A new method for the seismic design of plane steel moment resisting frames is developed. This method determines the design base shear of a plane steel frame through modal synthesis and spectrum analysis utilizing different values of the strength reduction (behavior) factor for the modes considered instead of a single common value of that factor for all these modes as it is the case with current seismic codes. The values of these modal strength reduction factors are derived with the aid of a) design equations that provide equivalent linear modal damping ratios for steel moment resisting frames as functions of period, allowable interstorey drift and damage levels and b) the damping reduction factor that modifies elastic acceleration spectra for high levels of damping. Thus, a new performance-based design method is established. The direct dependence of the modal strength reduction factor on desired interstorey drift and damage levels permits the control of deformations without their determination and secures that deformations will not exceed these levels. By means of certain seismic design examples presented herein, it is demonstrated that the use of different values for the strength reduction factor per mode instead of a single common value for all modes, leads to more accurate results in a more rational way than the code-based ones.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.775-783
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• 2005

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a $KBrO_3$ treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.

• Transactions on Electrical and Electronic Materials
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• 제9권5호
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• pp.209-212
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• 2008

We developed a simple and effective purification method to obtain high-purity single-walled carbon nanotubes (SWCNTs) with low surface damage. The purification process consists of oxidization at $430^{\circ}C$ for 1 h in a furnace system of air atmosphere and homogenization in dilute hydrochloric acid solution for extremely short time. The role of homogenizer was examined during purification process in terms of purity and quality of purified SWCNTs. High-purity and low surface damage of SWCNT products was obtained using homogenizer which was operated at 8500 rpm for 10 min in the environment of 7 % HCI solution. From XRD spectra, we observed that metal catalysts were thoroughly removed. Raman spectra showed that the intensity values of crystallization ($I_{G}/I_{D}$) of purified SWCNTs were very similar with that of pristine SWCNTs. Moreover, the structure damage of purified SWCNTs was hard to find from electron microscopy. Consequently, homogenizing, which is a quick and simple manner, can be promising method for obtaining final SWCNTs with clearly high purity and crystallinity.