• 대한물리치료과학회지
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• 제13권2호
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• pp.37-45
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• 2006

The purpose of this study was to determine the effect of treadmill training on gait, balance, and trunk control in a patient with hemiparesis. A female subject who had suffered a left hemiparesis 12 months previously was selected for this study. A single subject ABA design was used. Eight data-collection sessions were conducted during each of three phases (baseline-intervention-withdrawal). During baseline and withdrawal phases, the treatment based on Bobath approach was performed for the subject, and during the intervention phase, treadmill walking training was added. Assessment tools were made using the 10 m walk test, Rivermead Visual Gait Assessment(RVGA), Berg Balance Scale(BBS), and a seated Lateral Reach Test(LRT). During the intervention phase, the time measured in 10 m walk test and the scores of RVGA and BBS were significantly improved, and the number of steps in 10 m walk test and LRT showed a small improvement. During withdrawal phase, the time measured in 10 m walk test and the scores of RVGA and BBS were shown the carry-over effect. This findings indicate that treadmill training has significant effect to gait function and balance in a patient with chronic hemiparesis.

• 한국식품영양과학회지
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• 제38권11호
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• pp.1528-1534
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• 2009

구기자는 식용가능하고 제2형 당뇨병 합병증인 지방간분해, 고혈압 및 고혈당에 효능이 있다고 알려져 있다. 하지만 당 대사 및 지질 대사에 작용하는 정확한 기작에 대한 연구는 아직 미비하기에 탄수화물 소화효소인 ${\alpha}$-amylase와 ${\alpha}$-glucosidase의 저해활성 및 정상생쥐를 이용한 혈당강하 효과와 3T3-L1 adipocyte를 통하여 비만축적 억제효과를 보았다. 구기자 추출은 80% MeOH, 70% EtOH 및 열수추출하였으며 이들 중 ${\alpha}$-amylase 50% 저해 활성($IC_{50}$)은 saliva ${\alpha}$-amylase에서 acarbose, 80% MeOH, 70% EtOH, aqueous(7.04, 3.19, 3.20, 3.66 mg/mL), pacreas ${\alpha}$-amylase acarbose, 80% MeOH, 70% EtOH, aqueous(10.97, 4.16, 3.07, 3.55 mg/mL)로 saliva ${\alpha}$-amylase에선 acarbose보다 80% MeOH, 70% EtOH 추출물이 가장 낮은 농도에서 $IC_{50}$값을 나타냈고 pacreas ${\alpha}$-amylase에서는 70% EtOH 추출물이 가장 낮은 농도에서 $IC_{50}$값을 나타냈다. 또한 10 ${\mu}g/mL$ 농도에서 yeast ${\alpha}$-glucosidase 활성저해 $IC_{50}$값은 80% MeOH, 70% EtOH, aqueous(5.01, 4.98, 4.80 ${\mu}g/mL$)로 구기자 추출물 모두 acarbose(5.05)보다 우수한 $IC_{50}$값을 나타냈다. 정상생쥐에게 탄수화물 소화효소의 저해활성이 높은 70% EtOH 추출물을 전분과 함께 경구투여 한 후 내당능을 측정한 결과, 전분과 함께 100, 200 mg/kg body weight를 경구투여 한 경우, 대조군에 비해 투여 후 30, 60, 90, 120분에 혈당 증가가 낮았다. 또한 추출물을 100 mg보다 200 mg 투여하였을 경우 대조군에 비해 각각 19% 및 40%씩 저하되었으며, 120분에는 대조군 수준이 되었다. 그리고 3T3-L1 adipocyte에서 비만축적 억제효과를 측정한 결과 70% EtOH 추출물 농도가 증가함에 따라 leptin, 중성지방 농도가 감소하였고 유리지방산 농도는 증가하였다. 이상의 연구 결과 구기자 추출물 중 70% EtOH는 acarbose보다 높은 ${\alpha}$-glucosidase, ${\alpha}$-amylase 저해활성을 나타냈으며, 정상생쥐를 통해 식후 혈당증가를 저하시키는 효과를 확인하였고 GLUT4 발현 역시 증가함을 확인했다. 이를 통해 구기자 추출물이 지방축적 억제와 인슐린 저항성 억제 및 혈당강하 기전을 확인하여 제2형 당뇨병 개선에 효과적인 것으로 판단, 추후 더 연구하여 보다 더 정확한 성분을 조사할 필요성이 있다고 사료된다.

• Food Science and Biotechnology
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• 제17권1호
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• pp.130-134
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• 2008

Hypoglycemic effect of onion skin extract in vitro and in vivo was investigated. A methanol extract of onion skin inhibited yeast $\alpha$-glucosidase with an $IC_{50}$ of 0.159 mg/mL. A single oral administration of the onion skin extract (500 mg/kg) significantly lowered the postprandial area under the glucose response curve to starch (1 g/kg, p<0.05). Three-week-old db/db mice were fed an AIN-93G diet or a diet supplemented with a 0.5% onion skin extract for 7 weeks after a 1-week adaptation period. Consumption of onion skin extract significantly reduced the levels of plasma glucose, insulin, and blood glycated hemoglobin as compared with the control group (p<0.05). These findings suggest that onion skin is effective in controlling hyperglycemia in animal models of type 2 diabetes mellitus, at least in part by inhibiting $\alpha$-glucosidase activity.

• 전자공학회논문지
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• 제52권3호
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• pp.59-66
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• 2015

본 논문에서는 4bit SAR-ADC(Successive Approximation ADC) 기반의 LDO(Low Drop-Out Regulator)와 파워 트랜지스터의 사이즈 선택을 통하여 DC-DC 벅 컨버터의 효율을 개선하는 방법을 제안한다. 제안하는 회로는 부하 전류에 따라서 파워 트랜지스터 사이즈를 선택하여 DC-DC 벅 컨버터의 효율을 개선한다. 이를 위해, 우리는 스위칭 손실과 전도 손실이 교차하는 지점을 파워 트랜지스터의 적절한 사이즈로 선택하였다. 또한, standby mode 또는 sleep mode로 동작 시에는 효율을 개선하기 위해 LDO로 동작하도록 하였다. 제안하는 회로는 4bit로 파워 트랜지스터 사이즈(X1, X2, X4, X8)를 선택하였고, 저부하에서 단일 사이즈를 이용한 기존의 방식보다 최대 25%의 효율 개선을 얻을 수 있었다. 입력 전압은 5V, 출력 전압은 3.3V, 최대 부하 전류는 500mA이다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.765-768
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• 2013

Background and Aims: Prostate cancer is the most commonly diagnosed cancer in males in many populations. Metformin is the most widely used anti-diabetic drug in the world, and there is increasing evidence of a potential efficacy of this agent as an anti-cancer drug. Metformin inhibits the proliferation of a range of cancer cells including prostate, colon, breast, ovarian, and glioma lines. MicroRNAs (miRNAs) are a class of small, non-coding, single-stranded RNAs that downregulate gene expression. We aimed to evaluate the effects of metformin treatment on changes in miRNA expression in PC-3 cells, and possible associations with biological behaviour. Materials and Methods: Average cell viability and cytotoxic effects of metformin were investigated at 24 hour intervals for three days using the xCELLigence system. The $IC_{50}$ dose of metformin in the PC-3 cells was found to be 5 mM. RNA samples were used for analysis using custom multi-species microarrays containing 1209 probes covering 1221 human mature microRNAs present in miRBase 16.0 database. Results: Among the human miRNAs investigated by the arrays, 10 miRNAs were up-regulated and 12 miRNAs were down-regulated in the metformin-treated group as compared to the control group. In conclusion, expression changes in miRNAs of miR-146a, miR-100, miR-425, miR-193a-3p and, miR-106b in metformin-treated cells may be important. This study may emphasize a new role of metformin on the regulation of miRNAs in prostate cancer.

• 에너지공학
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• 제6권1호
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• pp.41-51
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• 1997

단일 드럼형 보일러를 갖는 발전 플랜트의 동특성 해석을 위한 프로세스 모델이 모듈 개념을 기반으로 묘사된다. 현재의 프로세스 모델은 플랜트 전체 구성 요소 및 고부하에서의 부하 변동을 포함하며 lumped parameter에 의해 물리적으로 접근된다. 플랜트 성분을 의미하는 모듈은 필수적으로 성분 특성을 잘 묘사하며 사용자에 의해 결정된 배열에 대해 압력점 방식에 의해 상호 연결된다. 프로세스 모델의 발전 플랜트 시스템에 대한 적용성 여부를 조사하기 위해 100MW발전 플랜트의 실제 운전 LOG-IC을 갖는 제어 시스템에 연결하여 3MW/min 부하 변동율로 75MW에서 95MW, 95MW에서 75MW조건에 대해 조사된다.

• 대한토목학회논문집
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• 제38권5호
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• pp.751-760
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• 2018

현재 다짐공사는 작업자가 정해진 다짐횟수, 두께, 속도, 진동여부 등을 자신의 경험에 의해 개별적으로 판단하고 감독자의 육안 검사에 의해 다짐공사의 품질측정이 이루어지고 있으며, 작업 후 대표성이 떨어지는 재래식 다짐강도 측정방법인 일점시험(Spot test)을 통하여 후속 단계의 작업을 진행하고 있는 실정이다. 따라서 다짐작업 결과에 대한 정량적 확인이 불가능하며, 시공부실로 인한 문제점이 제기될 가능성이 상존하고 있다. 본 연구에서는 여러 가지 시험조건에서 실제 현장규모로 다짐을 수행한 후 연속 다짐강도 측정 방법과 재래식 다짐강도 측정 방법과의 비교를 통해 연속 다짐강도 측정 방법의 사용 타당성을 입증하였다. 타당성 입증은 연속 다짐강도 측정방법인 GPS (Global Positioning System)와 가속도계로 구성된 IC (Intelligent Compaction) 롤러의 CMV (Compaction Meter Value)를 분석하는데 있으며, 입증된 결과를 토대로 일점시험을 배제한 현장 전 범위 품질확인이 가능하다. 품질 확인은 본 연구에서 개발한 다짐공사 관제프로그램에 시각화되며, 이를 통해 현장관리자는 다짐작업과 동시에 실시간 품질 모니터링이 가능하다.

• Restorative Dentistry and Endodontics
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• 제18권1호
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• pp.1-26
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• 1993

The purpose of this study was to investigate the effect of acid etching on the surface appearance and fracture toughness of five glass ionomer cements. Five kinds of commercially available glass ionomer cements including chemical curing filling type, chemical curing lining type, chemical curing metal reinforced type, light curing tilling type and light curing lining type were used for this study. The specimens for SEM study were fabricated by treating each glass ionomer cement with either visible light curing or self curing after being inserted into a rubber mold (diameter 4mm, depth 1mm). Some of the specimens were etched with 37% phosphoric acid for 0, 15, 30, 60, go seconds, at 5 minutes, 1 hour and 1 day after mixing of powder and liquid. Unetched ones comprised the control group and the others were the experimental groups. The surface texture was examined by using scanning electron microscope at 20 kV. (S-2300, Hitachi Co., Japan). The specimens for fracture toughness were fabricated by curing of each glass ionomer cement previously inserted into a metal mold for the single edge notch specimen according to the ASTME399. They were subjected to a three-point bend test after etching for 0, 30, 60, and 90 seconds at 5 minutes-, 1 hour-and 1 day-lapse after the fabrication of the specimens. The plane strain fracture toughness ($K_{IC}$) was determined by three-point bend test which was conducted with cross-head speed of 0.5 mm/min using Instron universal testing machine (Model No. 1122) following seven days storage of the etched specimens under $37^{\circ}C$, 100% humidity condition. Following conclusions were drawn. 1. In unetched control group, crack was present, but the surface was generally smooth. 2. Deterioration of the surface appearance such as serious dissolving of gel matrix and loss of glass particles occured as the etching time was increased beyond 15 s following Immediate etching of chemical curing type of glass ionomer cements. 3. Etching after 1 h, and 1 d reduced surface damage, 15 s, and 30s etch gave rough surface appearance without loss of glass particle of chemical curing type of glass ionomer cements. 4. Light curing type glass ionomer cement was etched by acid, but there was no difference in surface appearances according to various waiting periods. 5. It was found that the value of plane stram fracture toughness of glass ionomer cements was highest in the light curing filling type as $1.79\;MNm^{-1.5}$ followed by the light curing lining type, chemical curing metal reinforced type, chemical curing filling type and chemical curing lining type. 6. The value of plane stram fracture toughness of the chemical curing lining type glass ionomer cement etched after 5 minutes was lower than those of the cement etched after 1 hour or day or unetched (P < 0.05). 7. Light curing glass ionomer cement showed Irregular fractured surface and chemical curing cement showed smooth fractured surface.

• Nutrition Research and Practice
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• 제1권3호
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• pp.184-188
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• 2007

The major goal in the treatment of diabetes mellitus is to achieve near-normal glycemic control. To optimize both fasting blood glucose and postprandial glucose levels is important in keeping blood glucose levels as close to normal as possible. ${\alpha}-Glucosidase$ is the enzyme that digests dietary carbohydrate, and inhibition of this enzyme could suppress postprandial hyperglycemia. The purpose of this study was to test the inhibitory activity of methanol extract of Euonymus alatus on ${\alpha}-glucosidase$ in vitro and in vivo to evaluate its possible use as an anti-diabetic agent. Yeast ${\alpha}-glucosidase$ inhibitory activities of methanol extract of E. alatus were measured at concentrations of 0.50, 0.25, 0.10, and 0.05 mg/ml. The ability of E. alatus to lower postprandial glucose was studied in streptozotocin-induced diabetic rats. A starch solution (1 g/kg) with and without E. alatus extract (500 mg/kg) was administered to diabetic rats by gastric intubation after an overnight fast. Plasma glucose levels were measured at 30, 60, 90, 120, 180, and 240 min. Plasma glucose levels were expressed in increments from baseline, and incremental areas under the response curve were calculated. Extract of E. alatus, which had an $IC_{50}$ value of 0.272 mg/ml, inhibited yeast ${\alpha}-glucosidase$ activity in a concentration-dependent manner. A single oral dose of E. alatus extract significantly inhibited increases in blood glucose levels at 60 and 90 min (p<0.05) and significantly decreased incremental response areas under the glycemic response curve (p<0.05). These results suggest that E. alatus has an antihyperglycemic effect by inhibiting ${\alpha}-glucosidase$ activity in this animal model of diabetes mellitus.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.259-271
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• 2021

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatRI and CatRII regulate catBICIAI and catBIICIIAII in a cooperative manner, which provides new insights into naphthalene degradation.