• Journal of Korean Society on Water Environment
• /
• v.34 no.2
• /
• pp.210-215
• /
• 2018

It is difficult to count colonial cyanobacteria Microcystis cells since the thickness of colonies is constrained by amorphous mucilage, making it impossible to estimate the number of cells. Disaggregation of Microcystis colonies into single cell is needed to improve the accuracy and precision of cell density estimation of naturally collected samples. Uultrasonic treatment method is commonly used owing to the simplicity and immediacy of the procedure. However, amplitude, frequency, and duration of ultrasonic treatment also cause cell loss during the experiment. Optimal ultrasonic treatment has not been standardized yet. Therefore, the objective of this study was to investigate optimal ultrasonic treatment by analyzing cell density and colony numbers. We collected colonial Microcystis from Changnyeong-Haman weir area in Nakdong River during harmful algal boom period from September to October in 2017. Ultrasonic treatment method was applied to disrupt colonies into single cells to enumerate cell density. Among treatment conditions, results from continuously treated for 100 seconds were found to be the optimum to reduce colonies to a suspension of single cell without cell losses under high and low density of Microcystis cells. Lugol iodine fixed cells followed by sonication showed less negative impact of cell damage within the optimal treatment time (100 seconds). Furthermore, disaggregated cells treated by sonication enables microscopic observation more easily since gas vacuoles were collapsed to facilitate sedimentation of cells under the counting chamber for quantitative enumeration of buoyant Microcystis cells.

• Journal of the Korean Geotechnical Society
• /
• v.16 no.4
• /
• pp.31-42
• /
• 2000

In this study, the stability of group piles installed in deep sea to the seaquake was studied by performing the calibration chamber model tests for open-ended pipe piles, grouted piles under soil plug and close-ended piles installed in the simulated deep sea. For each case (a single pile, 2-pile and 4-pile groups), series of seaquake tests were performed. While, during the simulated seaquake, the compressive capacity of the single open-ended pile depended on pile penetration depth(=7m), were found to be stable. But, a single grouted pile with penetration depth of 13m kept "mobility" state, the one with penetration depth of 20m was stable and grouted pile groups with penetration depth of 7m were stable regardless of pile penetration depth. By grouting soil plug of open-ended piles and soil under the pile toe of open-ended pipe piles installed in the deep sea, failure of soil plugging was prevented. Thus, close-ended piles were more stable than open-ended pile against the seaquake motionake motion.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.21 no.5
• /
• pp.15-24
• /
• 2013

Numerical studies were conducted to investigate the internal flow field and combustion characteristics of DISI engine with methanol blended in gasoline. Dual injection was applied and the characteristics were compared to single injection strategy. The amount of the fuel injection was corresponded to air-fuel ratio of each fuel for complete combustion. The preforming model in this study, software STAR-CD was employed for both modeling and solving. The operating speed condition were at 4000 rpm/WOT (Wide open throttle) where the engine was fully warmed. The results of single injection with M28 showed that the uniformity, equivalence ratio, in-cylinder pressure and temperature increased comparing to gasoline (M0). When dual injection was applied, there was no significant change in uniformity and equivalence ratio but the in-cylinder pressure and temperature increased. When M28 fuel and single injection was applied, the CO (Carbon monoxide) and NO (Nitrogen oxides) emission inside the combustion chamber increased approximately 36%, 9% comparing with benchmarking case in cylinder prior to TWC (Three Way Catalytic converter). When dual stage injection was applied, both CO and NO emission amount increased.

• Korean Journal of Veterinary Research
• /
• v.38 no.4
• /
• pp.882-897
• /
• 1998

This experiment was conducted to predict the effects of motional characteristics on the fertility of Korean native cattle(KNC) by using CASA technology and in vitro fertilization system. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system(SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber(Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The oocytes collection, in vitro maturation, IVF, in vitro culture and determination of the cleavage rate were performed by the technique, as described by Hwang et al (1997). Statistical analysis was done by linear regression with use of the Sigma plot program on a IBM personal computer. The cleavage rate in vitro fertilized oocyte was significantly correlated(P<0.05) with MOT, VCL, VSL, VAP, ALH, BCF and MAD, but not CON, LIN, STR, WOB, DNM, DNC and HYP in regressional analysis. The results show that some kinematic characteristics of frozen-thawed semen by CASA can be predict the fertility in in vitro model system.

• Journal of the Korean Society of Tobacco Science
• /
• v.30 no.1
• /
• pp.1-7
• /
• 2008

Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tobacco in Korea. No effective single control measure is available at present time. One of the most potential way for controlling the bacterial wilt on tobacco is growing tobacco cultivars resistant to the bacterial wilt. In this study, optimal conditions for screening tobacco cultivars resistant to the bacterial wilt were examined to provide reproducible and efficient methods in growth chamber testing and field experiments for evaluating plant disease resistance. For this, already-known inoculation methods, inoculum densities, and incubation temperature, and plant growth stages at the time of inoculation were compared using tobacco cultivars resistant (Nicotiana tabacum cv, NC95), moderately resistant (N. tabacum cv. SPG70), and susceptible (N. tabacum BY4) to the bacterial disease. It was determined that root-dipping of tobacco seedlings at six true leaf stage into the bacterial suspension with inoculum level of $10^8$ colony-forming units (CFU)/ml for 20 min before transplanting was simple and most efficient in testing for resistance to the bacterial wilt of tobacco caused by R. solanacearum, for which disease incidences and severities were examined at 2 weeks of plant growth after inoculation at $20{\sim}25^{\circ}C$ in a growth chamber. These experimental conditions could discriminate one tobacco cultivar from the others by disease severity better than any other experimental conditions. In field testing, the optimum time for examining the disease occurrence was late June through early July. These results can be applied to establishing a technical manual for the screening of resistant tobacco cultivars against the bacterial wilt caused by R. solanacearum.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.02a
• /
• pp.230-230
• /
• 2013

In amorphous or microcrystalline thin-film silicon solar cells, p-i-n structure is used instead of p/n junction structure as in wafer-based Si solar cells. Hence, these p-i-n structured solar cells inevitably consist of many interfaces and the cell efficiency critically depends on the effective control of these interfaces. In this study, in-situ plasma treatment process of the interfaces was developed to improve the efficiency of a-Si:H solar cell. The p-i-n cell was deposited using a single-chamber VHF-PECVD system, which was driven by a pulsed-RF generator at 80 MHz. In order to solve the cross-contamination problem of p-i layer, high RF power was applied without supplying SiH4 gas after p-layer deposition, which effectively cleaned B contamination inside chamber wall from p-layer deposition. In addition to the p-i interface control, various interface control techniques such as thin layer of TiO2 deposition to prevent H2 plasma reduction of FTO layer, multiple applications of thin i-layer deposition and H2 plasma treatment, H2 plasma treatment of i-layer prior to n-layer deposition, etc. were developed. In order to reduce the reflection at the air-glass interface, anti-reflective SiO2 coating was also adopted. The initial solar cell efficiency over 11% could be achieved for test cell area of 0.2 $cm^2$.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.34 no.10
• /
• pp.71-79
• /
• 2006

Swirl injector with multi-stage tangential entry was analyzed to suppress high-frequency combustion instability in Liquid Rocket Engines. In order to analyze the effect of swirl injector as an acoustic absorber, swirl injector was regarded as a quarter-wave resonator and it's damping capacity is verified in atmospheric temperature. It has a finite mode of vibration and natural frequencies which can be tuned to the natural frequencies of a model combustion chamber. The interior air core shape of injector is more stable in the case of using the swirl injector with multi-stage entry than with single-stage entry. Also, when the swirl injector with multi-stage entry is used, tuned-injector length for unstable mode is well agreed with the calculated length. From the experimental data, it is proved that if the interior air core shape of swirl injector is stable, the fine tuned swirl injector can decrease the unstable mode of model chamber effectively and increase the damping rate.

• Journal of Veterinary Clinics
• /
• v.35 no.5
• /
• pp.233-236
• /
• 2018

A 13-year-old spayed female Beagle dog was referred with high intraocular pressure, hyperemia, and exophthalmos of the left eye and underwent ultrasound, which revealed a mass in the ciliary body of the left eye. Magnetic resonance (MR) imaging was ordered to evaluate invasion of surrounding structures and metastasis to the brain via the optic nerve. On MR imaging, a single, well-defined, smoothly marginated, triangular-to-oval-shaped mass was found. The mass was hyperintense on T1-weighted images and hypointense on T2-weighted images, similar to a previous case of ocular melanoma. The mass originated from the ciliary body and extended from the anterior chamber to the posterior chamber. Slight enhancement was observed in the mass. There was no evidence of invasion into surrounding structures or the optic nerve, and no sign of metastasis to the brain. The mass was histopathologically diagnosed as benign uveal melanocytoma.

• 유체기계공업학회:학술대회논문집
• /
• 2006.08a
• /
• pp.451-454
• /
• 2006

This paper describes the results of cold flow test and hot firing tests of an uni-element coaxial swirl injector and hot firing tests of a subscale combustor, as to the development effort of coaxial swirl injector for high performance liquid rocket engine combustor. A major design parameter for coaxial swirl injector is the recess number of a bi-swirl injector. The results of hot firing tests of the uni-element injector combustor and the sub-scale combustor are analyzed to investigate the effect of the recess number influencing on the combustion performance and pressure fluctuation. The test results of a cold flow test of the unielement combustor shows that it was shown that the change in recess number has significant effect on mixing characteristics and efficiency, while the effect of recess number on atomization characteristic is not The results of a series of firing tests using unielement and subscale combustor show that the recess length significantly affects the hydraulic characteristics, the combustion efficiency, and the dynamics of the liquid oxygen/kerosene bi-swirl injector. As a point of combustion performance, combustion efficiencies are 90% for unielement combustor and 95% for subscale combustor. The difference in the characteristic velocities between the unielement combustor and the subscale combustor may be caused by the difference in thermal loss to the combustor wall and the relative lengths of the combustion chamber. For a mixed type coaxial swirl combustor, the pressure drop across the injector increases as recess number becomes larger. The low frequency pressure fluctuation observed in unielement combustor can be related to the propellant mixing characteristics of the coaxial bi-swirl injector. The effect of the recess number on the pressure fluctuation inside the combustion chamber is more significant in un i-element combustor than the subscale combustor, of which the phenomena are also observed in time domain and frequency domain.

• Progress in Medical Physics
• /
• v.7 no.2
• /
• pp.57-65
• /
• 1996

Total Skin Electron Beam Therapy (TSEBT) is one of the most effective treatment methods for superficially disseminated skin cancer or cutaneous T-cell lymphoma. We have treated a patient with cutaneous T-cell lymphoma. We have used Stanford technique using six dual field. The nominal energy of electron beam was 4MeV. SSD was 390cm and the gantry angles of dual fields were 76$^{\circ}$ and 104$^{\circ}$. The dose profiles of single field and dual fields were measured with films and a Farmer type ion chamber. The field uniformity was 10％ over the patient's surface. During treatment, the patient was placed in six different positions for homogenous dose distribution over the body surface. The areas not directly exposed to the path of the electron beam (soles of feet, perineum and vertex of scalp) were boosted with 7MeV electron beam. During the treatment, lens, fingernails and toenails were shielded.