• 한국가금학회지
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• 제16권4호
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• pp.219-232
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• 1989

본 연구는 닭에 있어서 사료성분에 대한 췌장소화효소(amylase. trypsinogen 및 chymotrypsinogen) 분비기구에 대해서 검토했다. 먼저, 췌효소분비의 단기응답실험에 유용한 새로운 췌액채취법을 개발했다. 이 방법을 이용해서 아미노산 및 glucose를 날개정맥으로 투여한 결과 phenylalanine만이 trypsinogen 및 Chymotrypsinogen이 증가되었지만 그 외의 아미노산 및 glucose에 의해서는 분비증가 효과가 없었다. Cholecystokinin(CCK)투여 에 의해 췌효소분필는 즉각적으로 높은 분비반응을 보였으며, 이 반응은 또한 농도의존성을 나타냈다. CCK투여는 chymotrypsinogen의 쪽이 amylase 및 trypsinogen보다 높은 비율로 분비되는 선택적인 분비반응을 나타냈다. 아미노산과 CCK을 공동투여하면 첨가한 아미노산의 종류에 따라 췌효소분비반응은 여러 가지 형태로 증가되었지만 glucose와의 공동투여에서는 CCK 단독투여와 비교해서 차가 없었다. Valine과 arginine을 여러 가지 농도로 CCK와 공동 투여한 결과, valnine에서는 0.5mM일때, arginin에서는 5mM일때 가장 높은 분비반응을 보였다. 위의 결과로부터 아미노산의 조합에 의한 췌효소분비반응에 대해서 검토했다. 즉, 아미노산 mixture, threonine＋phenylalanine＋isoleucine, Threonine＋phenylalanine, threonine＋isoleucine 및 phenylalanine＋isoleucine과 CCK를 공동투여 했다. 각 물질을 투여한 후 50분간 분비한 효소를 비교하면, threonine＋phenylalanine에 의한 췌효소분비반응은 아미노산 mixture에 의한 분비반응과 동일하게 높은 반응을 보였다. 이상의 결과로부터 닭에 있어서 췌장소화효소분비는 CCK와 아미노산의 사이에 협동작용이 있으며, 그 협동작용은 아미노산의 종류에 따라 선택적인 분비반응을 함으로써 장내소화가 진행된다고 본다.

• 한국식품영양학회지
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• 제14권6호
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• pp.521-526
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• 2001

홍버섯쌀은 현재 혈압과 혈중 고지혈 강하제로서 의약품 뿐만 아니라 기능성 식품으로서 많은 관심을 불러일으키고 있다. 그러나 이 홍버섯에 함유된 유효성분인 monacolin K의 정성법과 정량적인 분석 방법이 제시되지 못하였다. 본 연구에서는 monacolin K의 lactone과 acid 형태에 대한 각각의 정확한 정성법과 정량가능한 분석법을 제안하였다. 이 방법은 HPLC의 역상컬럼을 사용하여 acetonitrile과 0.1%TFA를 62:38의 비율로 혼합한 용매를 사용하는 것이다. 이 제시된 방법을 활용했을 때 홍국중의 monacolin K의 최소 검출농도는 5ng이었다.

• 대한지하수환경학회지
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• 제1권1호
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• pp.6-9
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• 1994

지하수의 Ar/$N_2$가스의 비율은 탈질화(denitrification)의 정도를 측정하는 하나의 방법으로 사용된다. (Barnes et al., 1975; Vogel et al., 1981; Mariotti et al., 1988) 그 비율 측정은 일반적으로 질량분석기 (Mass Spectrometer)를 사용한다. 본 실험에서 사용한 Mass Spectrometer는 일반적인 Static Mass Spectrometer가 아닌 Ar과 $N_2$을 시간적으로 동시에 측정하여 오차를 줄일 수 있는 VG Optima isotope ratio Mass Spectrometer를 사용하였다. 두 지역 지하수의 Ar/$N_2$가스의 비율을 측정한 결과 Site 1(Grand Island, NE)의 경우 대기가스가 포화된 증류수의 Ar/$N_2$가스 비율인 0.026보다 낮은 0.015-0.018로서 탈질이 일어났음을 알 수 있으며, Site 2(Shelton, NE)의 경우 0.023-0.02로서 탈질이 일어나지 않음을 알 수 있다. 본 실험의 목적은 지하수에 응해되어 있는 Ar/Nitrogen의 비율을 정확히 측정하는 방법을 개발하므로서 탈질의 정도를 정확히 측정하며 탈질의 전개 과정을 관찰하기 위한 것이다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.1697-1702
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• 2003

In this paper, the effect of oxygen component in fuel on the exhaust emissions has been investigated for a direct injection diesel engine. It was tested to estimate change of engine performance and exhaust emission characteristics for the commercial diesel fuel and oxygenated blended fuel which has seven kinds of mixed ratio. And, the effects of exhaust gas recirculation(EGR) on the characteristics of NOx emission have been investigated. Ethylene glycol mono-n-butyl ether(EGBE) contains oxygen component 27% in itself, and it is a kind of effective oxygenated fuel of mono-ether group that the smoke emission of EGBE blended fuel is reduced remarkably compared with commercial diesel fuel, that is, it can supply oxygen component sufficiently at higher loads and speeds in a diesel engine. It was found that simultaneous reduction of smoke and NOx was achieved with oxygenated fuel and cooled EGR method.

• 생약학회지
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• 제45권2호
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• pp.113-120
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• 2014

Generally, Gwakhyangjeonggi-san has been used for treatment of diarrhea-predominant irritable bowel syndrome. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous quantification of marker compounds 1-20 in Gwakhyangjeonggi-san water extract. All analytes were separated by gradient elution using two mobile phases on a UPLC BEH $C_{18}$ ($100{\times}2.1mm$, $1.7{\mu}m$) column and maintained at $45^{\circ}C$. The injection volume was $2.0{\mu}L$ and the flow rate was 0.3 mL/min with detection at mass spectrometer. Regression equations of the compounds 1-20 were acquired with $r^2$ values ${\geq}0.9950$. The values of limit of detection and quantification of all analytes were 0.01-2.79 ng/mL and 0.03-8.37 ng/mL, respectively. The amounts of the compounds 1-20 in Gwakhyangjeonggi-san water extract were not detected $-3,236.67{\mu}g/g$. The established LC-MS/MS methods will be valuable to improve quality control of traditional herbal formula, Gwakhyangjeonggi-san.

• Fisheries and Aquatic Sciences
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• 제19권10호
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• pp.45.1-45.5
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• 2016

As a novel strategy to remove ${\beta}$-lactam antibiotic residues from fish tissues, utilization of ${\beta}$-lactamase, enzyme that normally degrades ${\beta}$-lactam structure-containing drugs, was explored. The enzyme (TEM-52) selectively degraded ${\beta}$-lactam antibiotics but was completely inactive against tetracycline-, quinolone-, macrolide-, or aminoglycoside-structured antibacterials. After simultaneous administration of the enzyme with cefazolin (a ${\beta}$-lactam antibiotic) to the carp, significantly lowered tissue cefazolin levels were observed. It was confirmed that the enzyme successfully reached the general circulation after intraperitoneal administration, as the carp serum obtained after enzyme injection could also degrade cefazolin ex vivo. These results suggest that antibiotics-degrading enzymes can be good candidates for antibiotic residue depuration.

• 생약학회지
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• 제42권2호
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• pp.138-143
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• 2011

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire $C_{18}$ column (4.6 mm${\times}$250 mm, 5 ${\mu}M$) was used at $40^{\circ}C$ for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 ${\mu}l$. The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.

• 한국자동차공학회논문집
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• 제14권5호
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• pp.25-31
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• 2006

Controlled Auto Ignition(CAI) combustion has great potential in achieving significant increase in engine efficiency, while simultaneously reducing exhaust emissions. The process itself involves the auto ignition and subsequent simultaneous combustion of a premixed charge. In this study, NVO(Negative Valve Overlap) system was applied to a CAI engine in order to use residual gas. The fuel was injected directly to the cylinder under the high temperature condition resulting from heating the intake port to initiate CAI combustion. This paper introduced the valve timing strategy and experimental set-up. From this study, the effect of engine speed and valve timing on CAI combustion and exhaust emissions was clarified. In addition, stratified charge method was used to extend CAI operating region.

• 동력기계공학회지
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• 제9권3호
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• pp.15-20
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• 2005

In this paper, the effect of oxygen component in fuel on the exhaust emissions has been investigated for an indirect injection diesel engine. It was tested to estimate change of engine performance and exhaust emission characteristics for the commercial diesel fuel and oxygenated blended fuel which has four kinds of mixed ratio. And, the effects of exhaust gas recirculation(EGR) on the characteristics of NOx emission have been investigated. Ethylene glycol mono-n-butyl ether(EGBE) contains oxygen component 27% in itself, and it is a kind of effective oxygenated fuel of mono-ether group that the smoke emission of EGBE is reduced remarkably compared with commercial diesel fuel, that is, it can supply oxygen component sufficiently at higher loads and speeds in diesel engine. It was found that simultaneous reduction of smoke and NOx was achieved with oxygenated fuel(10vol-%) and cooled EGR method(10%).

• 약학회지
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• 제58권3호
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• pp.158-164
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• 2014

Dangguisu-san is a well-known traditional Korean herbal medicine prescription and has been widely used to treat ecchymosis, blood stagnation, and pain resulting from physical shock in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous determination of the 17 biomarker components in Dangguisu-san. All analytes were separated on an UPLC BEH $C_{18}$ ($100{\times}2.1$ mm, $1.7{\mu}m$) column and maintained at $45^{\circ}C$. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in water (A) and acetonitrile (B) by gradient flow. The injection volume was $2.0{\mu}l$ and the flow rate was 0.3 ml/min with detection at mass spectrometer. Calibration curves of the 17 biomarker components were acquired with $r^2$ values ${\geq}0.9951$. The values of limit of detection and quantification of all analytes were 0.02~6.32 ng/ml and 0.05~18.95 ng/ml, respectively. The amounts of the 17 components in Dangguisu-san sample were $3.17{\sim}13,224.50{\mu}g/g$.