• Journal of Forest and Environmental Science
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• v.26 no.2
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• pp.117-129
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• 2010

The present study was undertaken in moist temperate forest of Mandal-Chopta area in the Garhwal region of Uttarakhand, India. The aim of the present study was to assess the physical properties of soils in relation to the forest structure and composition. Twelve forest types according to the altitude, slope aspect and species compositions were selected for the study. Physical properties of soil i.e., soil colour, soil texture (per cent of sand, silt and clay), moisture content, water holding capacity, porosity, bulk density (gm/$cm^3$) and void ratio were analyzed for three different depths viz., (i) 'upper' (0-10 cm), (ii) 'middle' (11-30 cm) and (iii) 'lower' (31-60 cm) in all the selected forest types. Phytosociological and diversity parameters viz. total basal cover ($Gha^{-1}$), stem density ($Nha^{-1}$), tree species richness, Simpson concentration of dominance and Shannon-Wiener diversity index were also calculated for each forest type. This study also provides the comparisons between the results of physical analysis of the present study with numerous other previous studies in the temperate Himalayan region of the Uttarakhand.

• Korean Journal of Weed Science
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• v.4 no.1
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• pp.11-18
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• 1984

The effect of transplanting method of rice seedlings on weed occurrence was investigated at the Yeongnam Crop Experiment Station in 1983. Rice was transplanted by hand and machine and eleven common herbicides were applied. The greatest weeds were harvested from the plot that rice was not transplanted and more weeds encountered at machine transplanted plot than hand transplanted plot. The degree of weed suppression by rice plant itself was 50% for machine transplatning and 55% for hand transplanting, respectively. Simpson index and community dominance of weed species were the highest in hand transplanting and followed by machine transplanting and no rice plot in order. Rice grain yield was exponentially correlated with the amounts of weed occurrence and more yield loss exhibited at machine transplanting than hand transplanting in the same amount of weeds. In the herbicidal activity, most of herbicides performed better at hand transplanting than machine transplanting while the degree of difference between transplanting methods varied depend on herbicide used.

• Journal of Microbiology
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• v.44 no.1
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• pp.106-112
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• 2006

In this study, the genetic diversities of multi-resistant Salmonella typhimurium (ST) isolates were analyzed via the application of both pulsed field gel electrophoresis (PFGE) and Polymerase chain reaction (PCR) analysis methods, using 6 kinds of primers (REP, ERIC, SERE, BOX, P-1254 and OPB-17). And their discriminative abilities (DA) were also compared in order to determine the most effective and reliable analysis method. 118 S. typhimurium isolates, cultured from diverse animals and human patients in Korea beginning in 1993, were analyzed and subjected to a comparison of Simpson's index of diversity (SID), using both PFGE and PCR methods. PFGE by XbaI enzyme digestion allowed for discrimination into 9 pulsotypes, with high SID values (0.991) on the genomic DNA level. This shows that PFGE is a very discriminative genotypic tool, and also that multiple clones of S. typhimurium isolates had existed in domestic animals and humans in Korea since 1993. However, we could ultimately not to trace the definitive sources or animal reservoirs of specific S. typhimurium isolates examined in this study. Depending on the SID values, the combined method (7 kinds of method) was found to be the most discriminative method, followed by (in order) SERE-PCR, REP-PCR, ERIC-PCR, PFGE & OPB-17 (RAPD), P-1254 (RAPD), and BOX-PCR at the $80\%$ clone cut-off value. This finding suggests that the REP-PCR method (which utilizes 4 primer types) may be an alternative tool to PFGE for the genotyping of S. typhimurium isolates, with comparable cost, time, and labor requirement. The establishment of a highly reliable and discriminatory method for epidemiologic analysis is considered necessary in order for researchers to trace the sources of specific pathogens and, consequently, to control and prevent the spread of epidemic S. typhimurium isolates to humans.

• Korean Journal of Weed Science
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• v.16 no.4
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• pp.259-263
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• 1996

Occurrence and distribution of weeds were surveyed in autumn vegetable crops(Chinese cabbage, radish, cucumber and Welsh onion) in 16 cities or counties of Chungnam area during October and November 1995. Thirty weed species were occurred, and Rorippa globosa, Alopecurus aequalis var. amurensis, Stellaria media, Portulaca oleracea, Digitaria sanguinalis and Chenopodium album var. centrorubrum showed higher importance values among them. S. media, P. oleracea and A, aequalis var. amurensis were dominant in northwest coastal area, R globosa, D. sanguinalis and S. media in southwest coastal area, R globosa, A. aegualis var. amurensis and D. sanguinalis in northern area, and R globosa, A. aequalis var. amurensis and P. oleracea in southern area. S. media, R globosa and P. oleracea were dominant in Chinese cabbage fields, R globosa, Amaranthus retroflexus and S. media in radish fields, A. retroflexus, S. media and D. sanguinalis in cucumber fields, and R. globosa and P. oleracea in Welsh onion fields.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.67-73
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• 2007

The present study compared the microbial diversity and activity during the application of various bioremediation processes to crude oil-contaminated soil. Five different treatments, including natural attenuation (NA), biostimulation (BS), biosurfactant addition (BE), bioaugmentation (BA), and a combined treatment (CT) of biostimulation, biosurfactant addition, and bioaugmentation, were used to analyze the degradation rate and microbial communities. After 120 days, the level of remaining hydrocarbons after all the treatments was similar, however, the highest rate (k) of total petroleum hydrocarbon (TPH) degradation was observed with the CT treatment (P<0.05). The total bacterial counts increased during the first 2 weeks with all the treatments, and then remained stable. The bacterial communities and alkane monooxygenase gene fragment, alkB, were compared by denaturing gradient gel electrophoresis (DGGE). The DGGE analyses of the BA and CT treatments, which included Nocardia sp. H17-1, revealed a simple dominant population structure, compared with the other treatments. The Shannon-Weaver diversity index (H') and Simpson dominance index (D), calculated from the DGGE profiles using 16S rDNA, showed considerable qualitative differences in the community structure before and after the bioremediation treatment as well as between treatment conditions.

• Journal of Ecology and Environment
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• v.31 no.4
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• pp.309-316
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• 2008

To develop a monitoring method for soil microbial communities in rice paddy fields, we used terminal restriction fragment length polymorphism (T-RFLP) to compare soil bacterial community structure in rice paddy fields experiencing different management practices: organic practices, conventional practices without a winter barley rotation, and conventional practices with a winter barley rotation. Restriction fragment length profiles from soils farmed using organic practices showed very different patterns from those from conventional practices with and without barley rotation. In principal component analyses, restriction fragment profiles in organic practice samples were clearly separated from those in conventional practice samples, while principal component analysis did not show a clear separation for soils farmed using conventional practices with and without barley rotation. The cluster analysis showed that the bacterial species compositions of soils under organic practices were significantly different from those under conventional practices at the 95% level, but soils under conventional practice with and without barley rotation did not significantly differ. Although the loadings from principal component analyses and the Ribosomal DNA Project II databases suggested candidate species important for soils under organic farming practices, it was very difficult to get detailed bacterial species information from terminal restriction fragment length polymorphism. Rank-abundance diagrams and diversity indices showed that restriction fragment peaks under organic farming showed high Pielou's Evenness Index and the reciprocal of Simpson Index suggesting high bacterial diversity in organically farmed soils.

• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Journal of Life Science
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• v.26 no.12
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• pp.1446-1457
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• 2016

Wetlands exhibit intermediate characteristics of both terrestrial and aquatic ecosystems, and the biodiversity is rich in these unique biological habitats. The symbiotic relationships between plants and fungi that inhabit these wetlands play an important role in natural resource management, biodiversity, and conservation. Accordingly, the mujechi, having academic value for the study of the natural environment, was investigated in terms of genetic diversity of endophytic fungi, which inhabit the roots of wild plants. The internal transcribed spacer (ITS) region was amplified to identify fungal strains. In total, 226 strains were isolated and categorized into three phyla, seven classes, 10 orders, 22 families, and 31 genera. In plants by endophytic fungi were classified in Isachne globosa (Ig) to 19 genera, Scirpus karuisawensis (Sk) to 11 genera, Utricularia racemosa (Ur) to 19 genera, and one incertae sedis, Eriocaulon decemflorum (Ed) to 11 genera. The fungal taxa was identified the genera Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), and Perenniporia (7.1%). The fungal group isolated from Isachne globosa (Ig) grew the largest number of isolated fungal strains. After comprehensive evaluation, the endophytic fungal group from Utricularia racemosa (Ur) ranked highest in diversity analyses. From the roots of wild plant in mujechi-neup, it confirmed the distribution and diversity of endophytic fungi. This study provides the basic data to understand fungal community structure in peat wetlands.

• Journal of Forest and Environmental Science
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• v.35 no.3
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• pp.159-172
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• 2019

Madhupur National Park (MNP) is one of the last remaining patches of old-growth natural Sal forest left in Bangladesh where the forest is tropical moist deciduous type. A study was revealed to assess the tree species diversity and composition in this area. For determining tree species the study was conducted through extensive random quadrat survey methods with $20m{\times}20m$ sized plots. Results of the study indicated that there were 139 tree species belonging to 100 genera and 40 families. The quadrat survey assessed the basal area, stem density, diversity indices and importance value index of the tree species having ${\geq}5cm$ D.B.H (Diameter at Brest Height). The basal area and stem density of the tree species were $20.689{\pm}1.08m^2/ha$ and $1412.93{\pm}64.27stem\;ha^{-1}$ while, diversity indices, i.e. Shannon-Wiener's diversity, Simpson's evenness, Margalef's species richness and Pielou's dominance indices indicated poor diversity in comparison to that of other PAs (Protected Areas) in South-Eastern region of Bangladesh. The structural composition based on height and D.B.H through reverse-J shaped curve indicated higher regeneration and recruitment but removal of trees of large growth classes. Sal (Shorea robusta) was the most dominant tree species that accounts 75% of the total tree individuals in the natural forest patches. However, some associates of Sal, i.e. Bhutum (Hymenodictyon orixensis), Gadila (Careya arborea), and Kusum (Schleichera oleosa) etc. were seemed to be rare in MNP.

• Mycobiology
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• v.46 no.2
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• pp.85-91
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• 2018

Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson's and Shannon's diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.