• 한국멀티미디어학회논문지
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• 제12권3호
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• pp.409-418
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• 2009

서비스 품질을 보장하기 위해 서비스 트래픽 흐름 경로상의 각 라우터는 자신에게 배분된 흐름의 지연규격을 준수해야 한다. 라우터에 가해지는 부하 량은 지연규격에 의해 결정되므로 라우터간 부하 불균형은 배분된 지연규격의 조정에 의해 해소될 수 있다. RSVP에 적용된 균등배분 방법은 간단하지만 자원 이용 효율이 낮다. 자원 이용 효율을 높이기 위해 라우터의 부하 상태에 따라 지연규격을 달리 배분하는 부하 밸런싱 방법이 연구되었으나 RSVP에 적용하기에 너무 복잡하다. 본 논문은 RSVP에 적용할 수 있으며 자원 이용 효율을 보다 개선할 수 있는 경로 부하 밸런성 방법을 제안한다. 제안된 방법은 흐름의 종단간 지연한계를 RSVP 절차에 의해 각 라우터에 배분한 후 경로의 병목상태와 링크의 대역폭 가용도에 따라 배분된 지연규격을 조정한다. 평가 네트워크를 대상으로 성능을 평가한 결과 제안방법은 기존방법에 비해 4 ${\sim}$ 17% 정도 높은 자원 이용 효율을 제공함을 보였다.

• 대한핵의학회지
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• 제28권1호
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• pp.106-111
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• 1994

2-iminothiolane is known to bind $NH_2$ group of lysine in the protein and deliver SH group, which can be used to label protein with $^{99m}Tc$. In this study, we looked for the best reaction condition in which 2-iminothiolane is conjugated to human polyclonal IgG and labeling condition with $^{99m}Tc$-glucoheptonate. Labeling yield was measured with TSK G4000SW column and HPLC or precipitation with 10% TCA (trichloroacetic acid) and 1% HSA. In vivo distribution was investigated with Staphylococcal abscess bearing rats. With decreasing glucoheptonate, the labeling yield decreased. Without 2-iminothiolane, $^{99m}Tc$-glucoheptonate was bound to IgG, which seemed to be direct labeling. With increasing 2-iminothiolane upto 20 times higher than IgG, the labeling yield increased, and plateau was seen with higher molar excess of 2-iminothiolane. Polymer formation was not observed. The pH for the conjugation of 2-iminothiolane and IgG was best around 6.4. $^{99m}Tc$-2-iminothiolane-IgG showed faster blood clearance, higher renal activity and lower hepatic and splenic activity than $^{99m}Tc$-DTPA-IgG. The biodistribution of $^{99m}Tc$-2-iminothiolane-IgG with higher molar excess of 2-iminothiolane was not different from that with lower molar excess. Labeling antibodies with $^{99m}Tc$ using 2-iminothiolane can afford a possible route to simple labeling and wide clinical use of the immunoscintigraphy.

• 한국작물학회지
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• 제30권1호
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• pp.47-52
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• 1985

Abscisin산(ABA)의 정량분석을 위한 방사면역 측정법을 개발하였다. ($\pm$)-ABA를 사람혈청 albumin에 conjugate 시킨 항원을 만들고 이를 가토에 접종하여 얻은 항혈청은 ABA에 대하여 높은 친화성을 나타냈다(Ka=3.28$\times$$10^{13}$$\ell$/mol ) $^3$H-ABA를 추적자로 사용하고 dextran-coated charcoal을 유리 ABA와 항체결합 ABA의 분리에 사용한 본 방사면역측정법은 ABA를 0.5$\times$$10^{-12}$mol 까지 측정할 수 있었고 측정범위는 14$\times$$10^{-12}$mol 까지 포함되었다. 이 면역측정법은 특이성이 높기 때문에 분석전 시료로부터 ABA를 미리 정제할 필요가 없었다. 시료 1개의 분석에는 2시간 정도 소요되어 하루 수백개의 시료률 분석처리할 수 있었다.

• Journal of Communications and Networks
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• 제13권6호
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• pp.602-611
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• 2011

The inter-domain routing scalability issue is a major challenge facing the Internet. Recent wide deployments of multihoming and traffic engineering urge for solutions to this issue. So far, tunnel-based proposals and compact routing schemes have been suggested. An implicit assumption in the routing community is that structured address labels are crucial for routing scalability. This paper first systematically examines the properties of identifiers and address labels and their functional differences. It develops a simple Internet routing model and shows that a binary relation T defined on the address label set A determines the cardinality of the compact label set L. Furthermore, it is shown that routing schemes based on flat address labels are not scalable. This implies that routing scalability and routing stability are inherently related and must be considered together when a routing scheme is evaluated. Furthermore, a metric is defined to measure the efficiency of the address label coding. Simulations show that given a 3000-autonomous system (AS) topology, the required length of address labels in compact routing schemes is only 9.12 bits while the required length is 10.64 bits for the Internet protocol (IP) upper bound case. Simulations also show that the ${\alpha}$ values of the compact routing and IP routing schemes are 0.80 and 0.95, respectively, for a 3000-AS topology. This indicates that a compact routing scheme with necessary routing stability is desirable. It is also seen that using provider allocated IP addresses in multihomed stub ASs does not significantly reduce the global routing size of an IP routing system.

• 한국축산식품학회지
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• 제33권4호
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• pp.501-507
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• 2013

Lysozyme was trapped from $2{\times}$ diluted egg white using Amberlite FPC 3500 ion exchange resin (1 g/10mL of egg white). The lysozyme bound to the resin was recovered using 0.1 N glycine-NaOH buffers, pH 9.0, containing 0.5 M NaCl. After separating lysozyme, the pH of the egg white solution was adjusted to 4.75 and centrifuged to remove interfering proteins. The supernatant was collected, added with 2.5% citric acid and 5.0% ammonium sulfate combination to precipitate egg white proteins, except for ovalbumin. After centrifugation, both supernatant (S1) and precipitant were collected. The precipitant was dissolved with 4 volumes of distilled water, and then 2.0% ammonium sulfate and 1.5% citric acid combinations added, stirred overnight in a cold room, and centrifuged. The resulting supernatant (S2) was pooled with the first supernatant (S1), desalted using an ultrafiltration unit, heat-treated at $70^{\circ}C$ for 15 min, and then centrifuged. The supernatant was collected as an ovalbumin fraction and lyophilized. The separated proteins were confirmed using Western blotting. The yield of lysozyme and ovalbumin was > 88.9% and > 97.7%, respectively, and the purity of lysozyme and ovalbumin was > 97% and 87%, respectively. The results indicated that the protocol was simple, and separated lysozyme and ovalbumin effectively.

• JSTS:Journal of Semiconductor Technology and Science
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• 제7권3호
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• pp.140-150
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• 2007

A simple, label-free microfluidic cell purification method is presented for separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and 50nm parallel lines with respect to the direction of flow) were fabricated using UV-assisted capillary moulding and included inside a polydimethylsiloxane (PDMS) microfluidic channel bonded onto glass substrate. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. It was found that the adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free detection and separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of $300{\mu}l/min$.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 추계학술대회 논문집 전기물성,응용부문
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• pp.53-54
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• 2006

Arsenic doped p-type ZnO thin films have been realized on intrinsic (100) GaAs substrate by RF magnetron sputtering and thermal annealing treatment. p-Type ZnO exhibits the hole concentration of $9.684{\times}10^{19}cm^3$, resistivity of $2.54{\times}10^{-3}{\Omega}cm$, and mobility of $25.37\;cm^2/Vs$. Photoluminescence (PL) spectra of As doped p-type ZnO thin films reveal neutral acceptor bound exciton ($A^{0}X$) of 3.3437 eV and a transition between free electrons and acceptor levels (FA) of 3.2924 eV. Calculated acceptor binding energy ($E_A$) is about 0.1455 eV. Thermal activation and doping mechanism of this film have been suggested by using X-ray photoelectron spectroscopy (XPS). p-Type formation mechanism of As doped ZnO thin film is more related to the complex model, namely, $As_{Zn}-2V_{Zn}$, in which the As substitutes on the Zn site, rather than simple model, Aso, in which the As substitutes on the O site. ZnO-based p-n junction was fabricated by the deposition of an undoped n-type ZnO layer on an As doped p-type ZnO layer.

• KSBB Journal
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• 제17권1호
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• pp.20-25
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• 2002

We performed a basic experiment for rapid, on-line, real-time measurement of HBsAg by using a surface plasmon resonance biosensor to quantify the recognition and interaction of biomolecules. We immobilized the anti-HBsAg polyclonal antibody to the dextran layer on a CM5 chip surface which was pre-activated by N-hydroxysuccinimide for amine coupling. The binding of the HBsAg to the immobilized antibody was measured by the mass increase detected by the change in the SPR signal. The binding characteristics between HBsAg and its antibody followed typical monolayer adsorption isotherm. When the entire immobilized antibody was interacted, there was no additional, non-specific binding observed, which suggested the biointeraction was very specific as expected and independent of the ligand density. No significant steric hindrance was observed at 17.6 nm/$mm^2$ immobilization density. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the chip surface was linear up to ca. $40\mu\textrm{g}$/mL, which is much wider than that of the ELISA method. It appeared the antigen-antibody binding was increased as the immobilized ligand density increased, but verification is warranted. This study showed the potential of this biosensor-based method as a rapid, simple, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification replacing the current ELISA method.

• Structural Engineering and Mechanics
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• 제17권5호
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• pp.691-706
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• 2004

The paper presents shear lag parameters for beam-to-column connections in steel box piers. Previous researches have analyzed beam-to-column connections in steel piers using a shear lag parameter ${\eta}_o$ obtained from a simple beam model, which is not based on a reasonable design assumption. Instead, the current paper proposes a cantilever beam model and has proved the effectiveness through theoretical and experimental studies. The paper examines the inaccuracy of the previous researches by estimating the effective width, the width-span length ratio L/b, and the sectional area ratio S of a cantilever beam. Two different shear lag parameters are defined using the cantilever model and the results are compared each other. The first type of shear lag parameter ${\eta}_c$ of a cantilever beam is derived using additional moments from various stress distribution functions while the other shear lag parameter ${\eta}_{eff}$ of a cantilever beam is defined based on the concept of the effective width. An evaluation method for shear lag stresses has been investigated by comparing analytical stresses with test results. Through the study, it could be observed that the shear lag parameter ${\eta}_{eff}$ agrees with ${\eta}_c$ obtained from the $2^{nd}$ order stress distribution function. Also, it could be observed that the shear lag parameter ${\eta}_c$ using the $4^{th}$ order stress distribution function almost converges to the upper bound of test results.

• 한국컴퓨터정보학회논문지
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• 제17권12호
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• pp.149-158
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• 2012

본 논문에서는 점근적으로 최적인 두가지의 무작위화 함수인 일라이어스(Elias) 함수와 페레즈(Peres) 함수의 장단점을 고려한 하이브리드 무작위화 함수를 제안한다. 무작위화 함수는 편향성이 있는 무작위수의 공급원으로부터 균등한 무작위수를 생성하는데 쓰이는 알고리즘을 수학적으로 추상화한 것이다. 일라이어스 함수와 페레즈 함수는 입력의 길이가 무한으로 증가함에 따라 그 출력효율성이 정보론적 한계치에 다가간다. 특히, 일라이어스 함수는 주어진 (유한의) 입력길이에 대해 최적인 무작위화 함수이다. 그러나 그 계산은 간단하지 않고, 주어진 입력길이에 의존한다. 반면, 페레즈 함수는 정해진 입력의 길이에 대해 출력효율이 최적이지는 않으나, 점근적으로는 최적이고, 간단한 재귀식에 의해 정의되어서 그 계산이 매우 간단하고 적은 메모리를 필요로 한다. 이러한 계산복잡도와 출력효율에 대한 두가지 무작위화 함수의 장단점에 주목하여, 각각의 장점을 고려한 하이브리드 무작위화 함수를 제안하고 이를 분석한다.