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A Study on Electrophoretic Pattern and Immunosuppressive Activity of Uterine Secretory Protein in Early Pregnant Sows (초기 임신돈에 있어서 자궁분비단백질의 전기영동상과 면역억제력에 관한 연구)

  • 김경순;정영채;김창근;한기영;김형태;윤종택;김종대;최선호
    • Korean Journal of Animal Reproduction
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    • v.13 no.3
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    • pp.179-187
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    • 1989
  • The experiment was carried out to study the profile of uterine specific protein during early pregnancy in sows and to test it's immunosuppressive activity. Uterine protein samples were obtained by flushing the uterine horn on Day 3, 6, 9, 12 and 15 of the estrous cycle and the pregnancy respectively and the protein concentration of each sample was determined. The change of uterine protein was examined by sodium dodecyl sulfate(SDS)-PAGE. The immunosuppressive activity of uterine secretory protein was investigated according to the lymphocyte blastogenesis response to mitogen. The results of this experiment are summarized as follows ; 1. The uterine protein during estrous cycle and early pregnancy was relatively constant up to Day 9, but increased on Day 12. Maxium total protein values were found on Day 15. The concentration of serum proteins were about 82-95 mg during estrous cycle, but decreased to about 70-82 mg during early pregnancy. 2. The proteins components similar electrophoretic patterns(PAGE) that were no differences (band ; a, b, c, d, e, f, g, I) on Days 3, 6 and 9 of the estrous cycle and pregnancy. But there were more 2 bands specifically on Day 12 of the pregnancy and on Day 15 of estrous cycle and showed more 4 bands on Day 15 of early pregnancy. They seemed to be acidoprotein and their average molecular weight were 38,000, 22,300 and 12,600. 3. When uterine protein were added 500$\mu\textrm{g}$/ml, there was no immunosuppresive activity on Day 3 of estrous cycle and lymphocyte blastogenesis was slightly suppressed on Day 3 of pregnancy. The immunosuppressive activity on Day 9 of estrous cycle and pregnancy appeared in 500$\mu\textrm{g}$/ml and 150$\mu\textrm{g}$/ml, respectively the uterine protein on Day 12 and 15 showed immunosuppresive activity, which at the level of 150$\mu\textrm{g}$/ml during non-pregnancy and at the level of 100 to 125$\mu\textrm{g}$/ml during early pregnancy, respectively.

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Quality Characteristics of Garaedduk with Lentinus edodes Powder (표고버섯분말을 첨가한 가래떡의 품질 특성)

  • Hyun, Young-Hee;Pyun, Jin-Won;Nam, Hae-Won
    • Korean journal of food and cookery science
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    • v.30 no.1
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    • pp.11-21
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    • 2014
  • This research focused on investigating the effect of lentinus edodes on the Garaedduk by analyzing its quality characteristics. Through this research, it could be verified whether this improved food has the potential to develop into a functional food. 5 pieces of Garaedduk with 0, 2, 4, 6, and 8% of lentinus edodes powder were analyzed by water content, color, sensory, textural characteristics and cooking characteristics right after the production and after one day. The five Garaedduk showed significant differences in water content during storage period of 0 & 1 day. As the result of Hunter's color values, with the increase of lentinus edode during storage period of 0 & 1 day, lightness was significantly decreased and redness and yellowness were significantly increased. As the result of textural analysis, hardness and chewiness showed significant increase and cohesiveness was significantly decreased in Garaedduk with the increase of lentinus edodes. When the Garaedduk were preserved for one day, the hardness and chewiness of the Garaedduk with 2% of lentinus edodes showed similar with the control. Cohesiveness showed significant decrease in all Garaedduk. The result of the sensory evaluation showed increase in the color, aroma and taste with the increase of lentinus edodes. The 2% Garaedduk showed similar hardness with the control. Garaedduk with lentinus edodes showed no difference in cohesiveness and adhesiveness with the control. The overall acceptability was significantly increased with the increase of lentinus edodes while the Garaedduk with 6% of lentinus edodes showed the highest overall acceptability. After one day of preservation, the Garaedduk with 4% lentinus edodes also showed the highest overall acceptability. As the result of cooking properties, weight gain and volume were significantly increased with the increase of lentinus edodes. The 2% Garaedduk particularly showed similar weight gain with the control. In turbidity of soup was significantly increased with the increase of lentinus edodes. In Hunter's color values of soup, lightness was significantly decreased with the increase of lentinus edodes. Redness and yellowness were significantly increased with the increase of lentinus edodes like Garaedduk.

A Case Study on the Meteorological Observation in Spring for the Atmospheric Environment Impact Assessment at Sangin-dong Dalbi Valley, Daegu (대기환경영향평가를 위한 대구광역시 상인동 달비골의 봄철 기상관측 사례분석)

  • Park, Jong-Kil;Jung, Woo-Sik;Hwang, Soo-Jin;Yoon, Ill-Hee;Park, Gil-Un;Kim, Sin-Ho;Kim, Seok-Cheol
    • Journal of Environmental Science International
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    • v.17 no.9
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    • pp.1053-1068
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    • 2008
  • This study aims to produce fundamental database for Environment Impact Assessment by monitoring vertical structure of the atmosphere due to the mountain valley wind in spring season. For this, we observed surface and upper meteorological elements in Sangin-dong, Daegu using the rawinsonde and automatic weather system(AWS). In Sangin-dong, the weather condition was largely affected by mountains when compared to city center. The air temperature was low during the night time and day break, and similar to that of city center during the day time. Relative humidity also showed similar trend; high during the night time and day break and similar to that of city center during the day time. Solar radiation was higher than the city, and the daily maximum temperature was observed later than the city. The synoptic wind during the measurement period was west wind. But during the day time, the west wind was joined by the prevailing wind to become stronger than the night time. During the night time and daybreak, the impact of mountain wind lowered the overall temperature, showing strong geographical influence. The vertical structure of the atmosphere in Dalbi valley, Sangin-dong had a sharp change in air temperature, relative humidity, potential temperature and equivalent potential temperature when measured at the upper part of the mixing layer height. The mixing depth was formed at maximum 1896m above the ground, and in the night time, the inversion layer was formed by radiational cooling and cold mountain wind.

Quality Characteristics of Jeolpyun with Hizikia fusiforme Powder (톳가루를 첨가한 절편의 품질 특성)

  • Pyun, Jin-Won;Hyun, Young-Hee;Nam, Hae-Won
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.196-204
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    • 2012
  • This research focused on the effects of adding Hizikia fusiforme to Jeolpyun. We were able to demonstrate in this study that Jeolpyun after the addition of Hizikia fusiforme had the potential to become a functional food. Jeolpyun with 0, 2, 4, 6, and 8% of Hizikia fusiforme was analyzed by water content, color, sensory and textural characteristics immediately after production and one day later. The five different types of Jeolpyun did not show any significant differences in water content. However, all the Jeolpyun with Hizikia fusiforme showed slightly higher water retention ratio than the control after storage. As the result of Hunter's color values, lightness decreased significantly as the amount of Hizikia fusiforme increased. Redness increased in the Jeolpyun with Hizikia fusiforme compared with the control. Yellowness was highest in the Jeolpyun with 2% of Hizikia fusiforme and decreased with an increase in its content. In the textural analysis, hardness, chewiness, adhesiveness and gumminess of Jeolpyun with 2% and 4% of Hizikia fusiforme were similar to those of the control, whereas springiness of Jeolpyun with 2%, 4% and 6% of Hizikia fusiforme were similar to that of the control group. Cohesiveness significantly decreased as the amount of Hizikia fusiforme increased. When the Jeolpyun was preserved for one day, all five Jeolpyun showed significant increases in hardness, gumminess and chewiness. However, their adhesiveness significantly decreased. Cohesiveness decreased in all Jeolpyun except for the 8% Jeolpyun. In sensory evaluation, color and aroma became stronger as the content of Hijikia fuziforme increased, and 2% Jeolpyun showed similar hardness with the control. Jeolpyun with Hijikia fuziforme showed no difference in cohesiveness with the control, but the adhesiveness showed a little increase when compared to the control. The overall acceptability of 2% Jeolpyun showed slightly higher than control. After one day of storage, Jeolpyun with 2% of Hijikia fuziforme also showed the highest overall acceptability. And the overall acceptability showed the biggest decrease in the control group. Overall acceptability showed strong negative correlation with color, aroma and hardness, and showed strong positive correlation with cohesiveness and adhesiveness. After one day of storage, overall acceptability showed a decrease in correlation with color and smell and an increase in correlation with cohesiveness, compared to before storage. Results of multiple regression analysis showed that hardness had the biggest influence(-) on the overall acceptability of Jeolpyun, aroma and cohesiveness ranked second(-) and third(+) respectively. After one day of storage, cohesiveness had the biggest influence on the overall acceptability of Jeolpyun. Color and adhesiveness ranked second(-) and third(+) respectively. It can be seen that different factors influence overall acceptability of Jeolpyun after storage.

The Effect of Microcurrent Stimulation on Wound Healing in Rat (미세전류전기자극이 흰쥐의 창상치유에 미치는 영향)

  • Oh, Hye-Jin;Kim, Jung-Woo;Park, Jang-Sung
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.6 no.1
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    • pp.43-55
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    • 2008
  • The purpose of this study was to investigate the effect of microcurrent stimulation intensity($50{\mu}A,\;100{\mu}A,\;300{\mu}A$ - 5 pps pulse frequency was same) on wound healing in rat. Sixty male Korean rats were randomly divided into 4 groups of 15 for 4 different treatment protocols(none-control group, $50{\mu}A,\;100{\mu}A,\;300{\mu}A$ experimental groups). Experimental 20 mm linear wound were made and all animals in the experimental groups were received microcurrent stimulation once a day for 20 minutes until sacrifice days(1st day, 3rd day, 6th day). A vernier caliper was used to measure a wound healing length and an optical microscope was used to determine any histological changes. The repeated measures two-way ANOVA was used for statistical differences in wound healing length. Experimental results were as follows: 1. In the examination with the naked eye, all groups showed similar changes until 1st day. But from 3rd day, a little intercellular fluid soaked through wound region in control group. In experimental groups, little intercellular fluid soaked through wound region, and swelling and redness did not appear. 2. Wound length of experimental $50{\mu}A$ group was significantly decreased than control group(p<0.001). And in the aspect of application period, wound length was significantly decreased in 3th, 6th day than 1st day and 6th day than 3th day (p<0.001). In conclusion, it has been found that the microcurrent stimulation had a positive effect on wound healing. And $50{\mu}A$ stimulation intensity was more effective than other intensities($100{\mu}A,\;300{\mu}A$) in wound healing. Also, low-intensity microcurrent stimulation was more effective on the purpose of wound healing.

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Treatment of Corn Starch Wastewater Using an UASB Reactor (UASB 반응조를 이용한 옥수수 전분폐수의 처리)

  • Shin, Hang-Sik;Bae, Byung-Uk
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.4
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    • pp.269-275
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    • 1993
  • The performance of Upflow Anaerobic Sludge Blanket(UASB) reactor for treatment of corn starch wastewater was investigated using continuous and batch experiment. Results showed that the corn starch wastewater had different characteristics in terms of biodegradability and methane potential, depending on the manufacturing precess. COD removal efficiencies were maintained over 70% up to the loading rate of 3.2 kg $COD/m^3{\cdot}day$ and the maximum gas production rate was about 55 l/day, equivalent to 3.5 l/day per liter of reactor volume, at the loading rate of 8.4 kg $COD/m^3{\cdot}day$. In the anaerobic serum bottle test(SBT) carried out along with continuous operation, the sludge activity was found to increase from 0.03 to 0.53 g $COD-CH_4/g\;VSS{\cdot}day$ as granular sludges were developed in 130 days operation. SBT gave valuable informations on the characteristics of wastewaters to be treated as well as on the sludge activity. The overall morphological characteristics of granular sludges cultivated on corn starch wastewaters were similar to those cultivated on various organic industrial wastewaters such as distillery and sugar.

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Effective application duration of sodium ascorbate antioxidant in reducing microleakage of bonded composite restoration in intracoronally-bleached teeth

  • Park, Jae-Young;Kwon, Tae-Yub;Kim, Young-Kyung
    • Restorative Dentistry and Endodontics
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    • v.38 no.1
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    • pp.43-47
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    • 2013
  • Objectives: The aim of this study was to determine an appropriate application duration of sodium ascorbate (SA) antioxidant gel in reducing microleakage of bonded composite restoration in intracoronally-bleached teeth. Materials and Methods: Eighty endodontically-treated human incisors were randomly divided into eight groups: control, no bleaching; IB and DB, immediate and delayed bonding after bleaching, respectively; S10m, S60m, S24h, S3d and S7d, bleaching + SA gel for 10 min, 60 min, 24 hr, 3 day and 7 day, respectively. For bleaching, a mixture of 30% hydrogen peroxide and sodium perborate was applied for 7 day. All access cavities were restored using One-Step adhesive (Bisco Inc.) and then Aelite LS Packable composite (Bisco Inc.). The bonded specimens were subjected to 500 thermal cycles, immersed in 1% methylene blue for 8 hr, and longitudinally sectioned. Microleakage was assessed with a 0 - 4 scoring system and analyzed using nonparametric statistical methods (${\alpha}$ = 0.05). Results: Group IB showed a significantly higher microleakage than the control group (p = 0.006) and group DB a statistically similar score to the control group (p > 0.999). Although groups S10m, S60m, and S24h exhibited significantly higher scores than group DB (p < 0.05), the microleakage in groups S3d and S7d was statistically similar to that in group DB (p = 0.771, p > 0.999). Conclusions: Application of SA gel for 3 day after nonvital bleaching was effective in reducing microleakage of composite restoration in intracoronally-bleached teeth.

Simulation of Truck-Loader Haulage Systems in an Underground Mine using GPSS/H (GPSS/H를 이용한 지하광산 트럭-로더 운반 시스템 시뮬레이션)

  • Park, Sebeom;Choi, Yosoon;Park, Han-Su
    • Tunnel and Underground Space
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    • v.24 no.6
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    • pp.430-439
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    • 2014
  • This study developed a simulation program for truck-loader haulage systems in an underground mine. A limestone underground mine in Korea was selected as a study area and investigated to design the simulation algorithm for truck-loader haulage systems. GPSS/H simulation language was utilized to develop the simulation program. Simulations were conducted to analyze the optimal number of haulage trucks dispatched in the 540 ML loading point by using the developed program. The simulation results showed that 8 trucks can maximize the daily production (2,076 ton). The real production (1,850 ton/day) of limestone ores was very similar to the simulated one (1,850.35 ton/day) when considers 7 trucks dispatched in the 540 ML loading point. In addition, average waiting time at loader were similar (waiting time by time study : 1.39 min, simulated waiting time : 1.35 min).

Quality Properties of Mortar Using the Recycled Fine Aggregates and Fly Ash Depending on Mixing Factors (순환잔골재와 플라이애시를 사용하는 모르터의 배합요인 변화에 따른 품질특성)

  • Han, Cheon-Goo;Son, Seok-Heon;Park, Kyung-Taek
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.5 no.4
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    • pp.99-105
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    • 2010
  • This study analyzed the effects of changes in mixing factors of zero-cement mortar that only used recycled fine aggregates and fly-ash on quality of mortar, and the results are as follows. To describe the property of fresh mortar, as mix proportion and flow of mortar increased, W/B was highly selected and air content decreased. To describe compressive strength according to age as the property of hardened mortar, it was revealed that the strength did not strength until the 14 day, but strength of about 1 ~ 2 MPa strength during the 14 day through 28 day, and almost similar strength after 28 day. Also, compressive strength according to changes in mix proportion, flow and B/W was generally similar. Summarizing the above experimental results, in case of mortar that used recycled fine aggregates and fly-ash, it secured the strength for the use of landfill even though not for structural use. Especially, poor mix proved to be more useful than rich mix.

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A Study on Protection Plan of Eutrophication in Fresh Water Environment by Development of Methods for Algal Growth Potential test (I) -Morphology and Growth Characteristics of Isolated algae- (조류생산잠재력조사 방법개발에 의한 육수환경의 부영양화 방지대책에 관한 연구(I) -순수분리종의 형태 및 증식특성-)

  • 위인선;나철호;이종빈;주현수
    • Journal of Environmental Health Sciences
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    • v.23 no.1
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    • pp.18-27
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    • 1997
  • The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

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