• Title/Summary/Keyword: Sim-Hak

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Determination of cyromazine residues in agricultural commodities using HPLC-UVD/MS (HPLC-UVD/MS를 이용한 농산물 중 Cyromazine의 잔류분석법)

  • Song, Lee-Seul;Kim, Young-Hak;Lee, Su-Jin;Hwang, Young-Sun;Kwon, Chan-Hyeok;Do, Jung-Ah;Oh, Jae-Ho;Im, Moo-Hyeog;Chang, Woo-Suk;Lee, Young-Deuk;Choung, Myoung-Gun
    • The Korean Journal of Pesticide Science
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    • v.16 no.3
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    • pp.202-208
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    • 2012
  • A high-performance liquid chromatographic (HPLC) method was developed to determine residues of cyromazine, a triazine insecticide, in agricultural commodities. Cyromazine was extracted with 90% aqueous methanol from representative crops which comprised brown rice, oyster mushroom, oriental melon, watermelon, and Chinese cabbage. Following to evaporation of methanol in the extract, the aqueous concentrate was acidified to form the protonated cyromazine. Dichloromethane partition was then applied to remove nonpolar co-extractives in the aqueous phase. Strong cation-exchange chromatography using Dowex 50W-X4 resin was employed for final purification of the extract. Cyromazine was successfully separated on a Zorbax SB-Aq $C_{18}$ column showing high retention for polar compounds. Cyromazine was sensitively quantitated by ultraviolet absorption at 214 nm. Limit of quantitation (LOQ) of the method was 0.04 mg/kg irrespective of sample types. Each crops were fortified at 3 different concentrations of cyromazine for recovery test. Mean recoveries from samples fortified at LOQ~2.0 mg/kg in triplicate ranged 80.2~103.3% in five agricultural commodities. Relative standard deviations in recoveries were all less than 6%. A selected-ion monitoring LC/MS method with electrospray ionization in positive-ion mode was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to routinely determine and inspect the residue of cyromazine in agricultural commodities.

Analysis of Postoperative Coronary Angiography in Symptomatic Patients (관상동맥 우회술 시행 후 증상이 있는 환자에서 시행한 관상동맥 조영술의 분석)

  • Kim Young-Hak;Han San-Woong;Kang Jeong-Ho;Kim Hyuck;Lee Chul-Burm;Chon Soon-Ho;Nam Seung-Hyuk;Chung Won-Sang
    • Journal of Chest Surgery
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    • v.39 no.10 s.267
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    • pp.759-764
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    • 2006
  • Background: We analyzed post-operative angiography performed in symptomatic patients to evaluate the patency rates and the roles of grafts. Material and Method: We reviewed 52 (15%) coronary angiograms performed for recurrent angina after prior coronary artery bypass surgery from January 1995 to June 2005. A total of 345 patients underwent coronary artery bypass surgery during this period. There were 41 men and 11 women and the mean age was $64.07{\pm}15.58$ years. The median period from operation to re-angiogram was 68.5 months (range, 1 to 126 months). The numbers of grafts and peripheral anastomoses were 42 and 43 for internal thoracic artery (ITA), 14 and 20 for radial artery (RA), and 49 and 89 for saphenous vein. The mean number of anastomosis was 2.9 per patient, Result: The patency rates of ITA, RA and saphenous vein graft (SVG) were 37/43 (86%), 17/20 (85%) and 34/89 (38.2%). The patency rate of arterial grafts was significantly higher than that of SVG (p< 0.001) and the patency rate of the RA was comparable to that of ITA (p=0.942). The patency rate of sequential SVGs was higher than that of single SVG (40.3% vs 31.8%, p=0.478) and the patency rate of proximal segments in sequential anastomosis was higher than that in single anastomsis (55.6% vs 31.8%, p=0.097), but statistically not significant. Conclusion: Arterial grafts have markedly superior patency rates than SVGs, so consideration should be given to the vigorous use of arterial grafts. The patency rate of the RA was comparable to that of ITA.

Comparison of Greenhouse Gas Emission from Liquid Swine Manure According to Aeration Levels in Summer (돈분뇨 액비의 폭기수준에 따른 여름철 온실가스 배출량 비교)

  • Choi, Dong-Yoon;Park, Kyu-Hyun;Cho, Sung-Back;Yang, Seong-Hak;Hwang, Ok-Hwa;Kwag, Jung-Hoon;Ahn, Hee-Kwon;Yoo, Yong-Hee
    • Journal of Animal Environmental Science
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    • v.17 no.3
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    • pp.163-170
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    • 2011
  • This study was carried out to investigate greenhouse gas (GHG), $CH_4$ and $N_2O$, emission from liquid swine manure according to aeration levels in summer. To evaluate the influence of operation methods on GHG emissions, liquid swine manure were applied with different rates of aeration (store without aeration, $1m^3/ton/h$, $2.5m^3/ton/h$, and $5m^3/ton/h$). Following are the results of this study. The liquid swine manure applied no aeration, $1m^3/ton/h$, $2.5m^3/ton/h$, and $5m^3/ton/h$ aeration rates released 315.6, 13.9, 17.9 and $9.6{\mu}g/m^2/s$ of $CH_4$ and 0.173, 0.157, 0.131, and $0.241{\mu}g/m^2/s$ of $N_2O$, respectively. Liquid swine manure applied no aeration released the most amount of GHG ($6,681.4{\mu}g/m^2/s$ $CO_2$-Eq.) and followed by $5m^3/ton/h$ ($276.4{\mu}g/m^2/s$ $CO_2$-Eq.), $2.5m^3/ton/h$ ($416.0{\mu}g/m^2/s$ $CO_2$-Eq.), and $1m^3/ton/h$ ($340.8{\mu}g/m^2/s$ $CO_2$-Eq.). Our results reveal that the aerated system may reduce GHG emissions compared to no aeration. Consequently, aeration and mixing were effective at reducing GHG emissions during liquid swine manure storage.

Evaluation of Basic Oxygen Furnace Slag as Soil Conditioner in the Soybean Upland Field (밭토양 콩재배에서 제강슬래그의 토양개량제로서의 시용 효과)

  • Lim, June-Taeg;Kim, Hee-Kwon;Park, In-Jin;Lee, Choong-Il;Hyun, Kyu-Hawn;Kwon, Byung-Sun;Kim, Hak-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.6
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    • pp.493-497
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    • 2000
  • An experiment was conducted to evaluate the possibility of using basic oxygen furnace (BOF) slag as soil conditioner in soybean upland field. In 1997, soybean (Glycine max L. cv. Eunha) crop was cultivated under different application rates of BOF slag at an experimental field of Chonnam Rural Development Administration in Nampyung, Najoo city. Five treatments, four application rates of BOF slag (0, 4, 8, $12Mg\;ha^{-1}$) and one application rate of lime ($2Mg\;ha^{-1}$) were tried with three replications. Plant height and shoot dry weight per plant were measured five times during the growth period. Chemical contents of soybean plant tissues and soil were also measured at the same sampling date. Yield were estimated by harvesting $6.6m^2$ per experimental unit and yield components were measured by sampling 10 plants per experimental unit at the harvest date. In upland soil, application of BOF slag rarely affected contents of total nitrogen, organic matter, available phosphate and potassium in soil. Soil pH, and contents of Ca and Fe in soil became higher as BOF slag rate increased. Enhancement of soil pH by application of BOF slag appeared to be closely related with increase in soil Ca content. Application rate of $2Mg\;ha^{-1}$ of lime showed almost the same effect in increase of soil Ca content as application rate of $4{\sim}8Mg\;ha^{-1}$ of BOF slag. Slag treatment hardly affected the contents of total nitrogen, $P_2O_5$, CaO, $K_2O$ and MgO in the shoot of soybean plants. Soybean plants under treatments of BOF salg showed better growth from the earlier growth stage compared with those of control treatment, and at the later growth stage, their growth was even superior to that of lime treatment. BOF slag rate of $8Mg\;ha^{-1}$ showed the highest soybean yield with $1,232kg\;ha^{-1}$. which was $330kg\;ha^{-1}$ or 37% higher than the yield of control with $902kg\;ha^{-1}$, As a result, BOF slag appeared to be useful material as a soil conditioner as well as nurient source for Ca and Fe in upland soybean fields, and its optimal rate for higher yield seemed to be around $8Mg\;ha^{-1}$.

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The Effect of Sympathectomy on Bone: -Evaluation with Quantitative Bone Scintigraphy- (흰쥐에서 교감신경절제술이 골에 미치는 영향 : -정량적 골스캔을 이용한 평가-)

  • Kim, Hak-Hee;Yang, Woo-Jin;Lee, Seong-Yong;Chung, Soo-Kyo;Park, Jang-Sang;Yim, Jung-Ik;Bahk, Yong-Whee;Shinn, Kyung-Sub
    • The Korean Journal of Nuclear Medicine
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    • v.28 no.1
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    • pp.85-88
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    • 1994
  • 근래 골조직에 있어서 자율신경의 기능에 대하여 많은 연구가 이루어지고 있으며, 골내의 자율신경의 해부학적 분포는 많이 알려져 있다. 그러나 임상적으로 반사적 교감신경 이상이나 레이노드 현상등과 같은 교감신경의 기능이상증에서나, 버거씨병 등의 치료 목적으로 시행되고 있는 교감신경 절제술 후, 자율신경기능의 변화가 사지골의 혈류나 골대사에 미치는 영향에 대하여는 아직도 논란의 여지가 있다. 저자들은 교감신경절제술 후 시간 경과에 따른 골에 미치는 영향을 알아보기 위하여 흰쥐에서 골대사와 혈류상태를 비교적 충실히 반영하는 정량적 골스캔을 시행하였다. 체중 $300{\sim}400g$의 수컷 흰쥐 10마리에서 복강을 통한 편측 요추부 교감신경절제술을 시행하였고, 수술 전과 후 1일, 3일, 1주, 2주, 3주, 4주에 양측 하지에서 각각 골스캔을 시행하고 교감신경 절제측 하지와 정상 하지에 대칭적으로 관심구역을 정하여 양측의 골스캔상 섭취계수를 비교하였다. 측정부위는 각 하지의 대퇴골간, 경골간 및 중족골로 하였다. 교감신경 절제술을 시행한 하지에서는 골스캔 소견상 수술 후 1일 또는 3일부터 동위원소 집적이 유의하게 증가되었으며 원위부로 갈수록 더욱 증가되었다. 그러나 3주 이후에는 정상측 수준으로 환원되었다. 교감신경절제술 후 골스캔상 동위원소집적이 증가되는 것은 골자체의 혈류가 증가되기 때문이며 이차적으로 골의 흡수를 유발하여 골밀도가 감소하는 것으로 생각되는데 이러한 변화는 시술 후 1일 째부터 관찰되어 사지골이 교감신경 절제에 매우 민감하게 반응하는 것을 알 수 있었다.9m}Tc$-MAA를 이용한 간 동맥 혈류 검사는 간암에서 색전술의 효과를 정확히 평가할 수 있는 유용한 검사법으로 이용될 수 있으리라 생각한다. 활성화 과정을 알아볼 수 있었으며 위상영상히스토그램을 통하여 이를 정량화하여 심실내 전기적 활성의 비동시성 여부를 추적관찰 할 수 있는 비관혈적검사임을 확인하였다.며, 3. $^{99m}Tc$으로 표지된 avidin과 streptavidin은 먼저 간으로 흡수된 후 대사된 다음 신장으로 배설된다는 사실을 알았다.damole에 의한 부작용은 흉통, 두통, 복통 등의 순이었고 전예에서 호전되었으며 생명에 위험을 초래할 수 있는 정도의 심장마비나 심부정맥은 한 예에서도 없었다. 결론적으로 dipyridamole은 약물부하 심근 SPECT 검사에 안전하게 사용할 수 있는 약물로 사료된다. 미소핵 빈도수가 증가하는 경향을 보였으나, 각 군간에 통계학적으로 유의한 차이는 없었다(p>0.05). 결론 : 임상적으로 치료를 중단하게 되는 1000mCi/60 Kg(16.67 mCi/Kg)를 투여한 군에서도 생쥐 골수내 미소핵이 발현되지 않는 것으로 보아, 방사성옥소는 비교적 안심하고 치료에 사용할 수 있는 제제로 사료되었다.반드시 비례하지만은 않아서 시간경과에 따른 추후 검사가 필요하리라 생각된다. 또한 방광요관역류가 있는 환아에서 DMSA 섭취율로 신기능을 평가할 때, 특히 영유아에서 연령에 따른 고려가 있어야 할 것으로 보인다.었다. 4) $^{99m}Tc-DISIDA$ hepatobiliary scintigram 음성율을

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Contamination of the Mushim-Cheon and its Countermeasure;II. The Status of the Seasonal and Hourly Contamination of the Water(1989${\sim}$1990)-Temperature, pH, DO, BOD, COD, SS, Turbidity, and BOD Load (무심천(無心川) 수질(水質) 오염(汚染) 실태(實態)와 그 방지책(防止策);II. 계절별(季節別) 및 시간별(時間別) 현황(現況) (1989-1990)-수온, pH, DO, BOD, COD, SS, 탁도 및 BOD 부하량(負荷量))

  • Lee, Jae-Koo;Kim, Hak-Nam;Kyung, Kee-Sung;Kwak, Hee-In
    • Korean Journal of Environmental Agriculture
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    • v.10 no.1
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    • pp.33-49
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    • 1991
  • In order to disclose the contamination of the Mushim-Cheon by pollutants and to establish the countermeasures, the water samples collected in November of 1989(lst sample), February(2nd), May(3rd) and August of 1990(4th) were analyzed to obtain the following results : 1. The water temperatures of the seasonal samples ranged from 8.6 to 16.2, 8.3 to 25.2, 18 to 26, and 24 to 32$^{\circ}C$, in the 1st, 2nd , 3rd, and 4th samples, respectively. 2. The pHs of all the samples ranged from 6.5 to 8.5. 3. In the 3rd sample, especially, DO was observed to fall down to 0.8 ppm at the downstream of St 13, which is not allowed even for agricultural use. 4. The BOD and COD values in sewers were much higher than those in the main stream, and especially the values of St 14-A reached 107-608 and 176-635 ppm, respectively, which far exceeded the limit of 40 ppm, the allowed value for the discharges from the disposal facilities. The SS value of St 14A ranged from 142 to 1, 900 ppm, which far exceeded the limit of 70 ppm, the allowed value for the discharges from the disposal facilities. 5. It turned out that the water quality of the Mushim-Cheon flowing through Cheong Ju was more worsened at sewers than the main stream, and at the downstream than the upstream of the surveyed area. Accordingly, the sewage disposal plant and the expansion of the disposal facilities are urgently needed.

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Preparation and Properties of Crosslinked Thermo-responsive Poly(N-isopropylacrylamide) Gel Materials For Smart Windows - Effect of Glycerol Content in Water/Glycerol Solvent - (스마트 윈도우용 가교 열감응성 폴리(N-이소프로필아마이드) 겔 소재의 제조 및 특성 - 물/글리세롤 혼합용매 중의 글리세롤 함량의 영향 -)

  • Park, Jae-Hyong;Kim, Il-Jin;Lee, Dong-Jin;Sim, Jae-Hak;Song, Min-Seop;Lee, Young-Hee;Yoo, Jung-Whan;Kim, Han-Do
    • Clean Technology
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    • v.24 no.2
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    • pp.112-118
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    • 2018
  • Thermo-responsive polymers that exhibit phase transition in response to temperature change can be used as materials for smart windows because they can control solar light transmission depending on the outside temperature. The development of thermo-responsive polymers for smart windows that can be used over a wide temperature range is desirable. To obtain high performance smart windows materials, three-dimensional thermo-responsive poly(N-isopropylacrylamide) (PNIPAm) gels were prepared by free radical polymerization from monomer N-isopropylacrylamide, N, N'-methylenebis acrylamide (MBAm) as a crosslinking agent, ammonium persulfate (APS) as a strong oxidizing agent/tetramethylene diamine as a catalyst, and a mixture of two solvents (water/glycerol). This study examined the effect of glycerol content on the lower critical solution temperature (LCST), freezing temperature and the solar light transmittance of crosslinked PNIPAm gel films. The LCST and freezing temperature of PNIPAm gel films were found to be significantly decreased from 34.3 and $6.3^{\circ}C$ to 28.2 and $-6.5^{\circ}C$ with increasing glycerol content from 0 wt% to 10 wt%, respectively. It was found that the transparent PNIPAm gel films at $25^{\circ}C$ (temperature < LCST) were converted to translucent gels at higher temperature ($45^{\circ}C$) (temperature > LCST). These results suggested that the crosslinked PNIPAm gel materials prepared in this study could have high potential for application in smart glass materials.

Effect of Defatted Soy flour on the Bread Making Properties of Wheat flour (탈지 대두분 첨가가 제빵 특성에 미치는 영향)

  • Yoo Yang-Ja;Chang Hak-Gil;Choi Young-Sim
    • Korean journal of food and cookery science
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    • v.21 no.3 s.87
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    • pp.301-310
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    • 2005
  • The effects of defatted soy flour on the physicochemical characteristics of dough and bread making properties were studied. Defatted soy flour is added to wheat flour for bread-making in order to maximize the use of isoflavones in the soybean. Different particle sizes of both defatted soy flour and wheat flour were prepared by grinding and sievingwith meshes. In the mixograph test, the addition of defatted soy flour to wheat flour increased the requirement for water and decreased the dough development time. Water absorption rates were also investigated to determine the optimum quantity of water for good dough. As the level of defatted soy flour mixed with wheat flour increased, the sedimentation and P.K. values decreased. In comparison with control, the bread made with defatted soy flour especially had a lower specific loaf volume. Specific loaf volume of wheat flour-defatted soy flour bread prepared (Ed- this is an incomplete sentence, it's only a subject clause, and I don't how what you intend to state). In terms of the staling rate and hardness of the wheat flour-defatted soy flour bread, the increased defatted soy flour had a faster staling rate during storage at 5? than at 25? for 5days. From the result of sensory evaluation, wheat flour-defatted soy flour breads containing up to $4\%$ defatted soy flour were rated as being of high quality.

The Clinical and Histopathologic Features according to Loss of LKB1 Protein Expression on Primary Lung Cancer (원발성 폐암에서 LKB1 단백질 발현 소실에 따른 임상 양상 및 조직병리학적 특성)

  • Hwang, Ki Eun;Jo, Hyang-Jeong;Lee, Kang Kyoo;Sim, Hyeok;Song, Jung Sup;Shin, Jeong Hyun;Shin, Seong Nam;Park, Seong-Hoon;Hong, Kyeong-Man;Park, Jung-Hyun;Jeong, Jong-Hoon;Kim, Hui Jung;Kim, Hak-Ryul;Yang, Sei-Hoon;Jeong, Eun-Taik
    • Tuberculosis and Respiratory Diseases
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    • v.64 no.5
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    • pp.362-368
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    • 2008
  • Background: LKB1(STK11) is a serine/threonine kinase that functions as a tumor growth suppressor. The functions of LKB1 in lung cancer are not completely understood. This study evaluated the relationship between LKB1 protein expression and the clinicopathological features in lung cancer tissues. Methods: The expression of LKB1 was studied in paraffin-embedded tumor blocks, which were obtained from 77 patients who had undergone surgery at Wonkwang University Hospital. The expression of the LKB1 protein was considered positive if the staining intensity in the tumor tissue adjacent to the normal airway epithelium was >30%. Results: The LKB1 expression was positive in 31 (40%) of samples. Loss of LKB1 expression was significantly associated with being male, smoking history, and squamous cell carcinoma. In the peripheral sites, the loss of LKB1 expression was strongly associated with a smoking history. A loss of LKB1 expression was more frequently associated with progression according to TNM staging, particularly more than T2, N progression. Conclusion: There was a significant relationship between the loss of the LKB1 protein and gender, smoking history, and histological type in primary lung cancer. Although LKB1 expression was not found to be a significant prognostic factor, further studies with a larger cohort of patient's lung cancer tissue samples will be needed to confirm this.

Studies on the development of mushroom mediums of Pleurotus eryngii using ginko leaf pomace (은행잎박을 이용한 큰느타리버섯 배지 개발)

  • Kim, Hong-Kyu;Kim, Yong-Gyun;Lee, Byung-Joo;Lee, Bong-Chun;Yang, Eyu-Seog;Kwon, Kyung-Hak;Kim, Hong-Gi
    • Journal of Mushroom
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    • v.7 no.3
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    • pp.105-109
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    • 2009
  • Ten to thirty percent of ginko leaf pomace(GLP) were added to pine sawdust to investigate mycelial growth. Mycelial growth on the medium with GLP was 86~101(mm/28 days) and was slower than that of the control without GLP as, 102(mm/28 days). Mycelial growth time on medium of sawdust with 10 to 20% GLP was similar to the control without GLP as 29 days but that on medium with 30% GLP was delayed for 2 days. The time of pinhead formations of sawdust with 10 to 20% GLP was 7 days, the growth time to harvest was 11 days. These results were similar to those of the control without GLP. The sizes of pileus of treatments with 10 to 30% GLP were tend to be smaller compared with the control. The length of stipe of 10% GLP treatment was 98mm which was longer than that of the control with 91mm. The thickness of stipes of 10 to 30% GLP treatments were 45 to 48mm which were tend to be thicker than that of the control with 45mm. The hardness of stipe were 35,062($g/cm^2$) to 4,1065($g/cm^2$)) which were harder than that of the control with 32,156($g/cm^2$)). GLP treatments seem to be favorable to store or distribute. Yields per volume of GLP added treatments were 88.7g to 95.6g, which was increased 3 to 12% compare to 85.8g of the control without GLP.

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