• 한국미생물·생명공학회지
• /
• 제39권1호
• /
• pp.77-85
• /
• 2011

본 연구는 은나노 입자의 항균활성을 알아보기 위하여, 그람 양성세균인 황색포도상구균과 그람 음성세균인 대장균에 대한 은나노 입자(Ag-NPs)를 처리 후, 세균세포 생장곡선측정, 활성산소생성능 측정, 세포질 단백질 누출량 측정, 젖산탈수소효소 활성측정 및 고분해능 임계방사 주사전자현미경 관찰이 수행되었다. 세균세포의 생장곡선 측정은 다양한 농도, 배양시간, 배양온도 및 pH에서 수행되었다. 결과적으로 황색 포도상구균과 대장균은 배양온도와 pH에 영향을 받지않고 은나노 입자에 의해 효과적으로 생장억제가 이루어지는 것을 관찰할 수 있었다. 또한 활성산소의 생성에 의하여 세포막의 파괴로 세포질내 물질의 유출을 세포질 유래 단백질 측정으로 확인할 수 있었으며, 젖산탈수소효소 활성측정을 통하여 은나노 입자에 대한 세포호흡억제활성 또한 확인할 수 있었다. 임계방사 주사전자현미경 관찰결과 은나노 입자에 의한 세균 세포표면의 형태학적 변화 또한 관찰되었다. 이러한 결과를 통하여 은나노 입자를 효과적인 항균활성소재로 활용 가능함이 입증되었다.

• 방사선산업학회지
• /
• 제5권2호
• /
• pp.119-124
• /
• 2011

Silver nano-particles (AgNPs) have attracted much attention for centuries due to their unique optical properties, electrical conductivities, oxidative catalysis, and antibacterial effect. In this study, AgNPs have been prepared by using aqueous $AgNO_3$ solution in the poly(vinyl alcohol) (PVA)/poly(ethylene glycol) (PEG) hydrogels. PVA and PEG powders were dissolved in deionized water, and then irradiated by a gamma-ray with a radiation dose of 50 kGy to make hydrogels. PVA/PEG hydrogels were dipped into $1.0{\times}10^{-2}M$ $AgNO_3$ solution for 1 hour. After that, the swollen hydrogels were irradiated by gamma-ray for the formation of AgNPs. FE-SEM is used to observe the formation of AgNPs as a function of the content of PEG and the irradiation dose. Also, AgNPs in the PVA/PEG hydrogels were monitored by UV-Vis. It is observed that the content of PEG and gamma-ray irradiation in the hydrogel is crucial to the formation of AgNPs. Finally, antibacterial tests indiacted that the hydrogel containing silver nanoparticle has antibacterial activity.

• Toxicological Research
• /
• 제35권3호
• /
• pp.257-270
• /
• 2019

Silver nanoparticles (AgNPs) have been widely used in a variety of applications in innovative development; consequently, people are more exposed to this particle. Growing concern about toxicity from AgNP exposure has attracted greater attention, while questions about nanosilver-responsive genes and consequences for human health remain unanswered. By considering early detection and prevention of nanotoxicology at the genetic level, this study aimed to identify 1) changes in gene expression levels that could be potential indicators for AgNP toxicity and 2) morphological phenotypes correlating to toxicity of HepG2 cells. To detect possible nanosilver-responsive genes in xenogenic targeted organs, a comprehensive systematic literature review of changes in gene expression in HepG2 cells after AgNP exposure and in silico method, connection up- and down-regulation expression analysis of microarrays (CU-DREAM), were performed. In addition, cells were extracted and processed for transmission electron microscopy to examine ultrastructural alterations. From the Gene Expression Omnibus (GEO) Series database, we selected genes that were up- and down-regulated in AgNPs, but not up- and down-regulated in silver ion exposed cells, as nanosilver-responsive genes. HepG2 cells in the AgNP-treated group showed distinct ultrastructural alterations. Our results suggested potential representative gene data after AgNPs exposure provide insight into assessment and prediction of toxicity from nanosilver exposure.

• Bulletin of the Korean Chemical Society
• /
• 제32권10호
• /
• pp.3581-3586
• /
• 2011

Silver nanoparticle (Ag-NPs)-immobilized and amine-functionalized carbon nanotubes (MWCNTs), MWCNT-Ag-$NH_2$, were easily prepared in order to develop an efficient delivery system of biomolecules without complicated processes of manufacture. For this, Ag-NPs-immobilized MWCNTs, MWCNT-Ag, were initially prepared in order to create large surface area to enable more efficient linkage with guest-molecules using pristine MWCNTs. The Ag-NPs on MWCNTs were further positively functionalized with 2-aminoethanthiol to allow ionic linkage with biomolecules. Ultimately, the positively charged delivery system proved to be highly effective for the binding capacity of bovine serum albumin (BSA) as a negatively charged model protein, when compared to that of lysozyme used as a positively charged model protein. The releasing profile of BSA was observed in almost linear pattern for about two weeks in a saline solution. This study demonstrated the potential usefulness of the pristine MWCNTs in conjunction with Ag-NPs for the selective delivery of many (negatively or positively) charged biomolecules including proteins and genes.

• Bulletin of the Korean Chemical Society
• /
• 제32권2호
• /
• pp.613-619
• /
• 2011

Colloidal silver nanoparticles (AgNPs) have been commercialized as the typically stabilized form via the addition of a variety of surfactants or polymers. Herein, to examine the effects of stabilizing AgNPs in suspension, we modified the surface of bare AgNPs with four type of surfactants (NaDDBS, SDS, TW80, CTAB) and polymers (PVP, PAA, PAH, CMC). The modified AgNPs was applied to compare suspension stability and nanotoxicity test using Escherichia coli (E. coli) as a model organism. Modification of AgNPs surface using chemical stabilizer may be not related with molecular weight, but chemical structure such as ionic state and functional group of stabilizer. In this study, it is noteworthy that AgNPs modified with a cationic stabilizer (CTAB, PAH) were importantly toxic to E. coli, rather than anionic stabilizers (NaDDBS, SDS). Comparing similar anionic stabilizer, i.e., NaDDBS and SDS, the result showed that lipophilicity of chemical structure can affect on E. coli, because NaDDBS, which contains a lipophilic benzene ring, accelerated the cytotoxicity of AgNPs. Interestingly, none of the stabilizers tested, including biocompatible nonionic stabilizers (i.e., TW80 and cellulose) caused a reduction in AgNP toxicity. This showed that toxicity of AgNPs cannot be reduced using stabilizers.

• Journal of Microbiology and Biotechnology
• /
• 제29권5호
• /
• pp.713-720
• /
• 2019

Acanthamoeba castellanii belonging to the T4 genotype may cause a fatal brain infection known as granulomatous amoebic encephalitis, and the vision-threatening eye infection Acanthamoeba keratitis. The aim of this study was to evaluate the antiamoebic effects of three clinically available antidiabetic drugs, Glimepiride, Vildagliptin and Repaglinide, against A. castellanii belonging to the T4 genotype. Furthermore, we attempted to conjugate these drugs with silver nanoparticles (AgNPs) to enhance their antiamoebic effects. Amoebicidal, encystation, excystation, and host cell cytotoxicity assays were performed to unravel any antiacanthamoebic effects. Vildagliptin conjugated silver nanoparticles (Vgt-AgNPs) characterized by spectroscopic techniques and atomic force microscopy were synthesized. All three drugs showed antiamoebic effects against A. castellanii and significantly blocked the encystation. These drugs also showed significant cysticidal effects and reduced host cell cytotoxicity caused by A. castellanii. Moreover, Vildagliptin-coated silver nanoparticles were successfully synthesized and are shown to enhance its antiacanthamoebic potency at significantly reduced concentration. The repurposed application of the tested antidiabetic drugs and their nanoparticles against free-living amoeba such as Acanthamoeba castellanii described here is a novel outcome that holds tremendous potential for future applications against devastating infection.

• Bulletin of the Korean Chemical Society
• /
• 제34권2호
• /
• pp.505-509
• /
• 2013

A low temperature ($65^{\circ}C$) thermal deposition process was developed for depositing a silver coating on thermally sensitive polymeric substrates. This low temperature deposition was achieved by chemical reduction of a silver alkylcarbamate complex with latent reducing agent. The effects of acetol as a latent reducing agent for the silver 2-ethylhexylcarbamate (Ag-EHCB) complex and their blend solutions were investigated in terms of reducing mechanism, and the size and shape of silver nanoparticles (Ag-NPs) as a function of reduced temperature and time, and PVP stabilizer concentration were determined. Low temperature deposition was achieved by combining chemical reduction with thermal heating at $65^{\circ}C$. A range of polymer film, sheet and molding product was coated with silver at thicknesses of 100 nm. The effect of process parameters and heat treatment on the properties of silver coatings was investigated.

• Bulletin of the Korean Chemical Society
• /
• 제33권3호
• /
• pp.906-910
• /
• 2012

Hollow gold-silver bimetallic nanoparticles (AuAg-HNPs) have been synthesized and their optical and structural properties were characterized. Initially Ag nanoparticles (Ag-NPs) were prepared using poly(ethylenimine) (PEI) as a reducing and a stabilizing agent simultaneously. AuAg-HNPs could then be synthesized via galvanic replacement reaction in a PEI aqueous solution by reacting sacrificial Ag template with a precursor compound of Au, i.e., $HAuCl_4$. Due to the presence of abundant amine functional groups in PEI, which could act as the dissolving ligand for AgCl, the precipitation problem of $Ag^+$ in the presence of Cl from $HAuCl_4$ salt was avoided. On this basis, the relatively high concentrations of $HAuCl_4$ and PEI-stabilized Ag nanoparticles could be used for the fabrication of AuAg-HNPs. Because of their increased surface areas and reduced densities, the AuAg-HNPs were expected and confirmed to outperform their solid counterparts in applications such as catalysis for the reduction of 4-nitrophenol in the presence of $NaBH_4$.

• 펄프종이기술
• /
• 제46권4호
• /
• pp.28-36
• /
• 2014

We studied the green synthesis and antibacterial activity of paper coated with chitosan-silver (Ag) green nanocomposites for packaging applications. Green synthesis of Ag nanoparticles (AgNPs) was achieved by a chemical reaction involving a mixture of chitosan-silver nitrate ($AgNO_3$) in an autoclave at 15 psi, $121^{\circ}C$, for 30 min. AgNPs and their formation in chitosan was confirmed by UV-Vis spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS). As-prepared chitosan-AgNPs composite materials were coated on manila paper using Meyer rod. Surface morphology and Ag contents in coating layer were characterized by field emission scanning electron microscopy (FESEM) and energy dispersive spectroscopy (EDS). The mechanical properties such as tensile strength and elongation were significantly affected by coating with chitosan-AgNPs. The antibacterial test of coated paper was performed qualitatively and quantitatively against Escherichia coli (E. coli). It was shown to be effective in suppressing the growth of E. coli with increasing Ag contents on the surface of coated paper and more than 95 R (%) of antimicrobial rate was obtained at chitosan-AgNPs coated papers.

• International Journal of Advanced Culture Technology
• /
• 제8권1호
• /
• pp.182-187
• /
• 2020

Silver nanoparticles (AgNPs) have been manufactured in recent years and widely used in various fields. Reactive oxygen species (ROS), which occur in AgNPs, destroy cell membranes. It is widely accepted that ROS generated in this manner inhibit microorganisms growth and causes toxic effects, However, it does not affect cell membranes directly but positively affects growth in plants with cell walls. The nanoball used in this experiment is a new material that generates ROS stably and is used in aqueous solution. Results of this study indicate a 30% increase in yield of Ginseng mixed with culture soil. The analysis of soil condition after cultivation showed that the possibility of repetitive cultivation in soil mixed with Nanoball was high. This suggests that Nanoball is an antimicrobial active material due to the microbial / extermination effect of pathogenic microorganisms. Therefore, there may be potential applications in agricultural cultivation sites as a repetitive cultivation technology that reuses soil.