• Korean Journal of Veterinary Research
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• v.36 no.3
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• pp.521-529
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• 1996

In an effort to obtain basic data of carbohydrate metabolism during spermiogenesis of the sexually-matured Jindo dog, the glycogen distribution in the testis was investigated by light and transmission electron microscopy. Periodic acid thiocarbohydrazide silver proteinate physical development(PA-TCH-SP-PD) staining method provided better results in the detection of glycogen granules from Sertoli cells and germ cells than the periodic acid schiff(PAS) staining method did. Pre-treatment of the tissue sections with ${\alpha}$-amylase elicited a significant decrease in PA-TCH-SP-PD stained granules, which suggested that the stained granules were of glycogen origin. High concentration of the glycogen granules were observed in the Sertoli cells, especially in its column, sheet-like processes, club-like processes, and tubular processes. The glycogen granules were unevenly distributed in some Sertoli cell columns. These results strongly indicated that the Sertoli cells of Jindo dogs showed vigorous activity of carbohydrate metabolism.

• Applied Microscopy
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• v.26 no.4
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• pp.411-420
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• 1996

A zinc-specific method (selenium method) has been employed to identify the zinc-containing cells in the cerebellum of the rats. When rats were allowed to survive 24 hours after the sodium selenite administration, zinc selenide reaction products formed in zinc-containing cellular boutons are retrogradely transported to the somata of those boutons. And the zinc selenide products accumulated in somata of the cells can be rendered visible by silver amplification of developer. Zinc-containing cells identified by the method were Bergmann glial and granule cells. Labeled zinc-containing cells were absent in molecular layer and white matter of the cerebellum. In ultrastructural level, the zinc selenide products were located in lysosomes of somata of the zinc-containing cells.

• Journal of the Korean Chemical Society
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• v.20 no.4
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• pp.277-279
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• 1976

A potentiometric titration method has been developed for the titration of copper with 2,5-dimercapo-1,3,4-thiadizole in aqueous solution using a silver electrode as an indicator electrode vs. the standard calomel electrode as a reference electrode. The 2,5-dimercapto-1,3,4-thiadiazole is very sensitive reagent for copper, which has been found to be highly selective for potentiometric determination of copper. Direct titration of trace amount of copper (0.02${\sim}$0.1 mg) is possible in the presence of a number of foreign ions in aqueous solution containing potassium hydrogen phthalate and sodium fluoride as masking agent under atmosphere.

• Journal of the Korea Safety Management & Science
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• v.12 no.2
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• pp.99-105
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• 2010

In this paper, the uAPSS(u-APartment Service System) that is based on location-aware technology is designed and implemented for a luxury apartment. On the real luxury apartment the developed system has been employed and tested to provide convenient and secure living for residents. It provides services such as emergency call, intelligent elevator operation, and hands-free door access based on the location of the residents with personal device as called smart tag. It can also be applied to other service areas such as the location-aware u-Service for hospitals, high-rising complex buildings, silver towns, etc.

• Journal of information and communication convergence engineering
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• v.1 no.1
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• pp.27-30
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• 2003

Electrophoretic deposition (EPD) of alcohol YBCO suspensions on the Ag wire electrode is studied. Poly(ethylene glycol) was coordinated to a structure formed by the EPD process with YBCO particles. The suspension is characterized in terms of zeta potential and conductivity. The d.c electric fields of 200-300 V/cm are applied for 1-10 min. The optimal condition for the EPD allows modifying the properties and microstructure of the deposited films. Superconducting coatings with nanometer-sized pores and a preferred orientation along the c-axis were prepared from the result with chemically modified precursor solution. In contrast, YBCO coatings of submicrometer-sized pores and randomly orientated grains were prepared from the solution without PEG.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.325-328
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• 2009

최근 자율 로봇을 지원하는 로봇의 지식 체계를 확립하기 위해 온톨로지 기술이 많이 사용되고 있다. 즉, 온톨로지를 통한 로봇의 지식 체계에 대한 데이터베이스 스키마를 설계하고 이에 따른 추론 시스템을 개발하는 연구가 활발히 진행되고 있는 시점이다. 이러한 관점에서, 본 논문은 독거 노인을 보조하는 실용적인 SilverMate 로봇분야와 같은 로봇의 지능적인 서비스를 요구하는 분야에서 로봇의 지식 체계 프레임워크를 제공하는데 초점을 두고 있다. 제안하는 지식 체계는 로봇의 자전적 기억을 중심으로 자전적 기억을 기술하기 위한 5W1H 모델을 제안하고 의미적 상관관계를 기술하기 위해 의미기억 모델로서 SCOT-SN 모델, 절차적 기억 모델로서 TES 모델을 정의한다.

• Journal of Practical Agriculture & Fisheries Research
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• v.7 no.1
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• pp.21-34
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• 2005

The purpose of this study was to suggest strategies for the development of mushroom associated tour villages with the aged. Mushroom has many strong points to use a theme for making a park and tour village. Especially it can be easily cultivated by the aged and a popularist commodity. The 5 days work system in a week enlarge the chance for the people to get a weekend tour. The number of the people who want to live in rural area after retire is getting increased also. This trend needs the development of tour villages as a kind of the silver agricultural industry in rural area. "Work & Play" concepts should be considered as a pivotal strategy to develope mushroom associated tour villages with the aged workforce. Mushroom can be a good theme attracting the tourist when the cultivating, showing, processing and marketing mushroom are welly connected in a village. And the traditional family game and culture must be adjusted for the tourist.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.6 no.2
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• pp.103-111
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• 2003

Purpose: This study was undertaken to evaluate the gastroduodenal pathology and Helicobacter pylori infection in children with upper gastrointestinal symptoms. Methods: One hundred and seven pediatric patients with upper gastrointestinal symptoms were undergone endoscopy at the Gyeongsang National University Hospital from June 1990 to April 1991. Histopathologic examination was done by H & E staining of gastric antral biopsy specimen and gastritis was defined according to the Sydney System. Tissue H. pylori status was evaluated with the urease test using Christensen's urea broth and H & E or Warthin-Starry silver staining of gastric antral biopsy specimen. IgG Immunoblotting were also performed to detect specific anti-H. pylori antibody in these patients. Results: The reasons for endoscopy were recurrent abdominal pain, acute abdominal pain, sallow face, hunger pain, and frequent nausea. Variable degrees of gastric mucosal hyperemia were found in most of the patients. Gastric hemorrhagic spots, gastric ulcer, duodenal ulcer, duodenal erosion, and hemorrhagic duodenitis were rare endoscopic findings. Histologic chronic gastritis was found in 88% of 107 patients. Histologic chronic duodenitis was observed in all 99 patients whose tissue were available. Gastric tissue H. pylori was positive in 57% of 107 patients by one of the ureasetest, H & E staining and Warthin-Starry silver staining. However, gastric tissue H. pylori detection rate was lower in the younger age groups. Anti-H. pylori IgG antibodies were detectable in 96% of 107 patients. Conclusion: Chronic gastroduodenitis and anti-H. pylori IgG antibody were ubiquitous in children with upper gastrointestinal symptoms.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.622-626
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• 2006

Molecular characterization of Hallikar, the native cattle breed of Karnataka, was undertaken using 19 cattle specific, highly polymorphic microsatellite markers recommended by FAO. The genomic DNA was subjected to PCR amplification and alleles were resolved through six per cent denaturing PAGE with a 10 bp DNA ladder followed by silver staining. Genotyping of animals was done based on allele size. The number of alleles ranged from three to nine with allele sizes ranging from 102 bp to 294 bp. These alleles were distributed in the frequency range between 0.0306 and 0.8673 in the population. The mean observed number of alleles was $6.368{\pm}1.4225$. The mean observed and expected heterozygosities were $0.7515{\pm}0.1734$ and $0.7850{\pm}0.1381$, respectively. The high heterozygosity observed implies presence of higher genetic variability within Hallikar breed. The PIC (Polymorphism Information Content) values ranged from 0.2322 (ETH152) to 0.8654 (ETH225). The percentage of polymorphic loci obtained was 100 as all the 19 microsatellite markers were found to be polymorphic. Except for ETH152, all the other loci had high PIC values, indicating that these markers are highly informative for characterization of Hallikar breed. The population was tested for Hardy-Weinberg equilibrium at 19 microsatellite loci, and at 74 per cent of the loci the population was found to be in disequilibrium.

• Journal of Gastric Cancer
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• v.1 no.3
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• pp.129-135
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• 2001

Purpose: Dysplasia or flat adenoma of the stomach is regarded as a precancerous lesion. However, the frequency and the evolutionary process of malignant transformation of gastric dysplasia are still debated. In order to see whether the lesion was a monoclonal or a polyclonal proliferation, clonality was assayed by X-linked HUMARA polymorphism. Materials and Methods: DNA was extracted from the paraffin-embedded tissue of 16 consecutive cases of endoscopic biopsy, eight of which supplied both dysplastic and nondysplastic tissue for comparison. HUMARA was amplified by PCR with or without pretreatment with methylationsensitive restriction enzyme, HpaII. The amplification products were electrophoresed on polyacrylamide gel and silver-stained. Results: Among the 16 cases, 13 cases were informative and 3 cases noninformative. Of the 13 cases, one case showed skewed lyonization, rendering 12 cases to be analyzed further. A monoclonal band pattern was noted in 2 cases, and a polyclonal band pattern in 10 cases. A review of the histopathologies of the monoclonal and the polyclonal cases did not reveal features discriminating the two groups. Conclusion: These results suggest that gastric dysplasia is a disease entity heterogeneous in the genetic level, and many cases may be non-neoplastic.