• The Korean Journal of Physiology and Pharmacology
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• 제24권3호
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• pp.203-212
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• 2020

Promyelocytic leukemia (PML) gene, through alternative splicing of its C-terminal region, generates several PML isoforms that interact with specific partners and perform distinct functions. The PML protein is a tumor suppressor that plays an important role by interacting with various proteins. Herein, we investigated the effect of the PML isoforms on oncostatin M (OSM)-induced signal transducer and activator of transcription-3 (STAT-3) transcriptional activity. PML influenced OSM-induced STAT-3 activity in a cell type-specific manner, which was dependent on the p53 status of the cells but regardless of PML isoform. Interestingly, overexpression of PML exerted opposite effects on OSM-induced STAT-3 activity in p53 wild-type and mutant cells. Specifically, overexpression of PML in the cell lines bearing wild-type p53 (NIH3T3 and U87-MG cells) decreased OSM-induced STAT-3 transcriptional activity, whereas overexpression of PML increased OSM-induced STAT-3 transcriptional activity in mutant p53-bearing cell lines (HEK293T and U251-MG cells). When wild-type p53 cells were co-transfected with PML-IV and R273H-p53 mutant, OSM-mediated STAT-3 transcriptional activity was significantly enhanced, compared to that of cells which were transfected with PML-IV alone; however, when cells bearing mutant p53 were co-transfected with PML-IV and wild-type p53, OSM-induced STAT-3 transcriptional activity was significantly decreased, compared to that of transfected cells with PML-IV alone. In conclusion, PML acts together with wild-type or mutant p53 and influences OSM-mediated STAT-3 activity in a negative or positive manner, resulting in the aberrant activation of STAT-3 in cancer cells bearing mutant p53 probably might occur through the interaction of mutant p53 with PML.

• 한국멀티미디어학회논문지
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• 제20권10호
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• pp.1662-1670
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• 2017

A-Scan NDT equipment is widely used in the industrial field because it is inexpensive and easy to carry but it is necessary to have a skilled inspection specialist who is trained to analyze the waveform of ultrasonic signal. Since the welding quality is judged subjectively by the specialist, there is a problem in the reliability of the quality. In the C-Scan NDT which overcomes the shortcomings of the A-Scan, welding part can be represented in the form of two dimensional image by combining one dimensional ultrasonic waveform so that the quality of welding can be grasped without the help of specialist. In order to develop C-Scan NDT, it is necessary to develop an array type two dimensional transducer and an algorithm to composing image by combining ultrasonic signals generated from a two dimensional transducer. In addition, the noise component must be minimized in the ultrasonic signal in order to display the quality of welding in the form of images. Therefore we propose a method to remove noise component from the ultrasonic wave and construct a two dimensional ultrasonic image.

• Natural Product Sciences
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• 제21권3호
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• pp.205-209
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• 2015

Fucoidan, a sulfated polysaccharide is found in several types of edible brown algae. It has shown numerous biological activities; however, the molecular mechanisms on the activity against atopic dermatitis have not been reported yet. We now examined the effects of fucoidan on chemokine production co-induced by TNF-α/IFN-γ, and the possible mechanisms underlying these biological effects. Our data showed that fucoidan inhibited the TNF-α/IFN-γ-induced production of thymus and activation-regulated chemokine (TARC) and macrophagederived chemokine (MDC) mRNA in human keratinocytes HaCaT cells. Also, fucoidan suppressed phosphorylation of nuclear factor kappa B (NF-κB) and activation of signal transducer and activator of transcription (STAT)1 in a dose-dependent manner. In addition, fucoidan significantly inhibited activation of extracellular-signal-regulated kinases (ERK) phosphorylation. These data indicate that fucoidan shows anti-inflammatory effects by suppressing the expression of TNF-α/IFN-γ-induced chemokines by blocking NF-κB, STAT1, and ERK1/2 activation, suggestive of as used as a therapeutic application in inflammatory skin diseases, such as atopic dermatitis.

• Journal of Photoscience
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• 제9권2호
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• pp.102-105
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• 2002

Phoborhodopsin (pR or sensory rhodopsin II, sRII; the absorption maximum of ∼ 500 nm) is a retinoid protein and works as a photoreceptor of the negative phototaxis of Halobacterium salinarum. pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. These sensory proteins form a complex with a cognate transducer protein in the membrane, and this complex transmits the light-signal to the cytoplasm to evoke avoidance reaction from blue-green light. Recently, the functional expression in Escherichia coli membrane of ppR was achieved, which can afford a large amount of the protein and enables mutant studies to clarify the role of various amino acid residues. A truncated transducer which can bind to ppR is also expressed in Escherichia. coli membrane. In this article, we will review properties of ppR mainly using observations of our laboratory; which contains photochemistry (photocycle), light-driven proton uptake, release and transport, F -helix titling during photocycle and association of the transducer.

• 센서학회지
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• 제27권4호
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• pp.269-274
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• 2018

High-sensitivity signal-to-noise ratio (SNR) microphones are essentially required for a broad range of automatic speech recognition applications. Piezoelectric microphones have several advantages compared to conventional capacitor microphones including high stiffness and high SNR. In this study, we designed a new piezoelectric membrane structure by using the finite elements method (FEM) and an optimization technique to improve the sensitivity of the transducer, which has a high-quality AlN piezoelectric thin film. The simulation demonstrated that the sensitivity critically depends on the inner radius of the top electrode, the outer radius of the membrane, and the thickness of the piezoelectric film in the microphone. The optimized piezoelectric transducer structure showed a much higher sensitivity than that of the conventional piezoelectric transducer structure. This study provides a visible path to realize micro-scale high-sensitivity piezoelectric microphones that have a simple manufacturing process, wide range of frequency and low DC bias voltage.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2006년 창립20주년기념 정기학술대회 및 국제워크샵
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• pp.41-45
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• 2006

The fabrication process of fiber placement system of carbon fiber reinforced plastic (CFRP) requires real time process control and reliable inspection to ensure quality by preventing defects such as delamination and void. Therefore, novel non-contact inspection technique is required during the non-destructive evaluation in a fiber placement system. For the inspection of delamination in CFRP, various methods to receive laser-generated ultrasound were applied by using piezoelectric transducer, air-coupled transducer, wavelet transform and scanning laser ultrasonic technique. Laser-generated ultrasound was received with a conventional piezoelectric sensor in contacting manner. Then signal characteristics due to defects were analyzed to find a factor for detecting defects. Air-coupled transducer was used for reception of laser-generated guided wave using linear slit array in order to generate high frequency guided wave. And line scan technique was used to confirm the capability of on-line application. The high frequency component of laser-generated guided wave received with piezoelectric sensor disappeared after propagating through delamination region. Nevertheless, it was failed to receive high frequency guided wave in using air-coupled transducer. The first peak of the frequency spectrum under 100kHz in the delamination region is higher than in the sound region. By using this feature, the line scanned frequency data were acquired in fully non-contact generation and reception of ultrasound. This method was proved as useful technique for detecting delamination in CFRP.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 하계학술대회 논문집
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• pp.942-945
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• 2000

Recently, electret applications have placed the focus on polymer thin film because electrical properties of a blend are studied. In this paper, we hale measured the infra sonic frequency by using Electret Sensor. Electret is formed by appling the voltage range of -4[kV] to -8[kV] to PTFE film and the sensor is manufactured by moment method to detect the infra-sonic signal. Electret Infrasonic Transducer, which is designed and manufactured by using of the moment method according to the potential and electric field simulation , shows its promising result, since the average rising rate of sensitivity is 7.68 [dB/oct] under 1[Hz], and the average values are within ${\pm}$ 1 [dB/oct]. As a result, it is believed that the characteristics of acquired transducer can be applied to the medical treatment, the industry, and the animal life researches, and also the study on noise elimination is required.

• 전기학회논문지
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• 제58권4호
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• pp.867-872
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• 2009

Peak expiratory flow rate(PEF) is one of the most important diagnostic parameters in spirometry. PEF occurs in a very short duration during the forced expiratory maneuver, which could lead to measurement error due to non-ideal dynamic characteristic of the transducer. In such case the initial slope of the flow rate signal determines the accuracy of the measured PEF. The present study considered this initial slope as a parameter to compensate PEF. The 26 standard flow rate signals recommended by the American Thoracic Society(ATS) were flown through the air flow transducer followed by simultaneous measurements of PEF and maximum transducer output$(N_{PEF})$. $N_{PEF}$-PEF satisfied a quadratic equation in general, however, two signals (ATS #2 and #26) having large initial slopes deviated from the fitting equation to a significant degree. The relative error was found to be in a linear relationship with the initial slope, thus, $N_{PEF}$ was appropriately compensated to provide accurate PEF with mean relative error less than only 1%. The 99% confidence interval of the mean relative error was less than a half of the error limit of 5% recommended by ATS. Therefore, PEF can be very accurately determined by compensating the transducer output based on the initial slope, which should be a useful technique for air flow transducer calibration.

• 비파괴검사학회지
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• 제29권2호
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• pp.93-97
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• 2009

자기변형 탐촉자는 유도초음파를 적용한 장거리 배관 검사 시에 유용하게 사용되는데 특히 그 신호패턴이 배열형 압전탐촉자를 사용한 유도초음파 신호보다 깨끗하고 단순하기 때문에 단일 결함에 의한 신호패턴을 해석할 수 있을 뿐만 아니라 결함 신호의 진행 상황을 분석함으로써 배관의 구조 건전성 감시(structural health monitoring)에도 사용되고 있다. 그러나 실제 현장 검사에서는 유도초음파의 방향성신호, 잔향효과, 모드변환, 구조물 등에 의한 의사 신호 또는 거짓 신호가 나타나기 때문에 결함 평가 시에 주의를 요한다. 본 연구에서는 실제 상황을 모의한 장거리배관 mock-up에서 발생하는 모드변환 신호를 실험적으로 검증하고 해석하였다. 자기변형 스트립 탐촉자에서 발생한 비틀림진동 T(0,1) 모드와 휨진동 F(1,3) 모드 또는 종진동 L(0,2) 모드 간에 모드변환이 발생함을 확인하였고 이러한 모드변환이 발생하는 원인을 스트립 탐촉자 및 검사대상체의 전자기적 재료 특성 및 구조 측면에서 해석하였다.

• 비파괴검사학회지
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• 제32권1호
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• pp.1-6
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• 2012

본 연구에서는 광대역(100 kHz - 1 MHz) 탐촉자를 사용하여 자동차용 CNG연료탱크의 파열시험시 발생하는 음향방출신호의 분석을 통해 압력용기의 손상정도를 평가하였다. 압력이 420 bar까지 올라가기 전의 각 단계에서는 Kaiser 효과가 나타나고 creep 효과가 거의 나타나지 않으나, 420 bar 이상 상승시 creep 효과가 현저히 나타남으로써 용기가 손상을 입었다는 것을 알 수 있었으며, 480 bar 압력 상승 단계에서는 Kaiser 효과가 없어졌다. 540 bar의 압력 단계에서 공진형 탐촉자의 경우에서는 480 bar의 압력단계에 비하여 energy나 count 같은 activity가 감소하였으나 광대역 탐촉자의 경우 계속적으로 증가하였다. 또한 rise time이나 주파수 분석을 통해서 복합재료 압력용기의 손상 메카니즘을 관찰하기 위해서는 일반적으로 금속압력용기에서 많이 사용되는 공진형 탐촉자(150 kHz)보다 광대역 탐촉자가 효과적임을 알 수 있었다.