• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.553-559
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• 1996

We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

• Journal of KIISE:Software and Applications
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• v.34 no.7
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• pp.595-602
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• 2007

This paper proposes a method that discriminates signal peptide and predicts the cleavage site of the secretory proteins cleaved by the signal peptidase I. The preprocessing stage uses hydrophobicity scales of amino acids in order to predict the presence of signal sequence and the cleavage site. The preprocessing enhances the performance of the prediction method by eliminating the non-secretory proteins in the early stage of prediction. for the effective use of support vector machine for the signal sequence prediction, the biologically relevant distance between the amino acid sequences is defined by using the hydrophobicity and substitution matrix; the hydrophobicity can be used to Predict the location of amino acid in a cell and the substitution matrix represents the evolutionary relationships of amino acids. The proposed method showed 98.9% discrimination rates from signal sequences and 88% correct rate of the cleavage site prediction on Swiss-Prot release 50 protein database using the 5-fold-cross-validation. In the comparison tests, the proposed method has performed significantly better than other prediction methods.

• Journal of Korean Society of Transportation
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• v.20 no.7
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• pp.23-29
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• 2002

Five and more leg intersections have been still in operation in many urban areas. The number of conflicts in five leg intersection is more than four leg intersection. The signal timing design in the five leg intersection should be performed not only to reduce delay but also to increase safety. This paper suggests safety enhanced signal phase sequence design of a rotary with five leg intersection such as phase sequence minimizing the number of conflict points at the rotary with five leg intersections and the phase-length-design procedure by utilizing the Traffic Network Study Tool(TRANSYT). Field data was collected from Gonguptap five leg intersection in Ulsan and TRANSYT-7F was applied for signal timing design model. Optimal signal phase length and sequence of TRANSYT-7F is rearranged based on the Principal of "two moving traffic flows per phase". In conclusion, proposed signal phase design increased delay by 6.2% compared with the optimal signal phase of TRANSYT-7F. However, it could decrease the number of conflict in the five leg intersection by 61.5%.

• The Journal of the Acoustical Society of Korea
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• v.9 no.2
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• pp.42-52
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• 1990

A method of measuring impulse response of acoustic system, two kinds of cross-correlation methods (the direct correlation method and the M-sequence modulation correlation method) were applied. According to the direct correlation method, by using stationary random noise source and by calculating the cross-correlation function between the sourece and the output signal, equivalent impulse response can be obtained not being influenced by the back ground noises. By applying this method, the measurement of echo-time patterns in rooms and oblique incident sound absorption characteristics of sound absorbing materials was carried out. In the case of the M-sequence modulation correlation method which was contrived by Aoshima, an intermittent random nosie modulated by M-sequence signal is used as the source signal, and the cross-correlation function between the M-sequence signal and the squared output signal is calculated. According to this method, equivalent energy impulse response (squared impulse response) of te propagation system can be obtained without being influenced by the back ground noises and the air fluctuation caused by wind. As the applcaition of this measuring method to the architectural acoustics, the meaurements of echo-time patterns, reverberation decays and sound pressure lev디 distributions in rooms and sound insulation efficiencies in buildings were carried out. From these experimental studies, it has been found that this M-sequence modulation correlation method is markedly useful especially for the field masurement of sound insulation under high back ground noise condition.

• Proceedings of the KIEE Conference
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• 2001.11c
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• pp.376-379
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• 2001

In this paper, I design the control chip that controls inner test sequence of Logic Tester to test chip. Logic tester has the thirteen inner instructions to control test sequence in test. And these instructions are saved in memory with test pattern data. Control chip generates address and control signal such as read, write signal of memory. Before testing, necessary data such as start address, end address, etc. are written to inner register of control chip. When test started, control chip receives the instruction in start address and executes, and generates address and control signals to access tester' inner memory. So whole test sequence is controlled by making the address and control signal in tester's inner memory. Control chip designs instruction's execution blocks, respectively. So if inner instruction is added from now on, a revision is easy. The control chip will be made using FPGA of Xilinx Co. in future.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2570-2575
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• 2003

Monitoring a power transmission line is significant for power electric companies. In this paper, we propose a new method for detecting an fault point of power transmission line by use of M-sequence correlation technique. In this method, detecting signal is used as one or plural M-sequences ( same characteristic polynomial, including normal and reverse mark, synchronized ). In receiving point, we make same sequence with the input one and take crosscorrelation function between M-sequence and the received signal. We can see transfer fanctions of plural paths between inputs and a output taps separated from different of delay times on the crosscorrelation function, and from these transfer fanctions, so we compare them when fault occurred with in usual.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.8
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• pp.836-844
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• 1991

PLC(programmable logic controller) system is widely used for the control of factory. PLC system receives ladder diagram which is drawn by the user to implement hardware logic, converts the ladder diagram into sequence program which is executable in the PLC system, and executes the sequence program indefinitely unless user breaks. The sequence program processes the data of on/off signal, and endures 1 scan delay and missing of pulse-type signal shorter than a scan time. So, data dependency doesn't exist. By applying theis characteristics to multiprocessor architecture, we design parellel PLC functionally and evaluate performance upgrade. Parallel PLC consists of central processing module, N general processing unit, and a shared memory by master-slave type. Each module executes allocated sequence program by the control of central processing module. We can expect performance upgrade by parallel processing, and reliability by relocation of sequence program when error occurs in processing module.

• ETRI Journal
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• v.28 no.1
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• pp.95-98
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• 2006

In this letter, a novel M-ary code-selected direct sequence (DS) ultra-wideband (UWB) communication system is presented. Our purpose is to achieve a high data rate by an M-ary code-selected direct sequence bipolar pulse amplitude modulation (MCSDS-BPAM) scheme. In this system, a particular DS code sequence is selected by the $log_2M$/2 bits from the DS gold code set. This scheme can accomplish both a high data rate without increasing the system bandwidth or changing the pulse shape and improve the BER with an increase of modulation level M even at a lower signal-to-noise ratio (SNR). The receiver signal processing algorithm is given for an MCSDS-BPAM UWB system over an ideal AWGN channel and correlation receivers.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.15-16
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• 2006

In this paper, we propose an adaptive SLM scheme based on peak observation for PAPR reduction of OFDM signals. The proposed scheme is composed of three steps: peak scaling, sequence selection, and SLM procedures. In the first step, the peak signal samples in the IFFT outputs of the original input sequence are scaled down. In the second step, the sub-carrier positions where power difference between the original input sequence and the FFT outputs of the scaled signal is large, are identified. Then, the phase sequences which have the maximum number of phase-reversed sequence words only for these positions, are selected. Finally, only using the selected phase sequences, the generic SLM procedure is performed for the original input sequence. Simulation results reveal that the proposed adaptive SLM remarkably reduces the complexity in terms of IFFT and PAPR calculations than the conventional SLM, while maintaining the PAPR reduction performance.

• Korean Journal of Microbiology
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• v.31 no.3
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• pp.203-207
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• 1993

Five different secretion vectors were constructed by varying the signal sequences and .alpha.-antitrypsin (.alpha.1-AT) a numan secretory protein, was produced from yeast cells. The signal sequences used are those of acid phosphatase (PH05) and .alpha.-factor (M f.alphal1) of Saccharomyces cerevisiae, glucoamylase (STA1) of Saccharomyces diastaticus, and human .alpha.1-AT. Four vectors directed the efficient secretion of .alpha.1-AT ito the culture media. The secretion vector carrying the glucoamylase signal sequence (pGAT11) showed the highest efficiency of secretion. About 70% of .alpha.1-AT produce dwere secreted into the media. The endo H treatment of partially purified .alpha.1-AT indicates that the secreted .alpha.1-AT appeared to be glycosylated. This glycosylation pattern was altered when amino acid substitution mutations were introduced at the three glycosylation sites of .alpha.-AT.