• Korean Journal of Microbiology
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• v.30 no.2
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• pp.108-112
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• 1992

To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, .alpha.-1-antitrypsin (.alpha.-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the .alpha.1-AT expression vector pGAT6 which contained .alpha.-1-AT cDNA fused to GAL10-CYC1 promotor. The .alpha.-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was onfirmed both by the .alpha.-1-AT activity assay and by the immunoblot method using .alpha.-1-AT antibody. We also showed the secretion of .alpha.-1-AT into the culture media and into the periplasmic space by immunoblot.

• Journal of Digital Convergence
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• v.20 no.1
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• pp.185-190
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• 2022

Bluetooth is a common wireless technology that is widely used as a connection medium between various consumer electronic devices. The Bluetooth receiver usually adopts a Viterbi algorithm to improve signal-to-noise ratio performance, but requires complex hardware and calculations for continuous search and estimation for the irrational modulation indexes at the transmission. This paper proposes a non-coherent maximum estimation based 8-State Viterbi FSM to solve these complexity problems. The proposed optimal Viterbi FSM can detect Gaussian frequency-shfit keying symbol without any prior information and estimation for the modulation indexes. The HV1/HV2 packets are used for the estimation of the proposed algorithm and the simulation results have shown performance improvements with about 2dB for 10^-3 BER compared to other ideal approaches such as decision direct method.