• Molecular & Cellular Toxicology
• /
• v.5 no.3
• /
• pp.216-221
• /
• 2009

Bisphenol A (BPA) is commonly used in the production of pharmaceutical, industrial, and housing epoxy, as well as polycarbonate plastics. Owing to its extensive use, BPA can contaminate the environment either directly or through derivatives of these products. BPA has been classified as an endocrine disruptor chemicals (EDCs), and the primary toxicity of these EDCs in males involves the induction of reproductive system abnormality. First, in order to evaluate the direct effects on the Y chromosome associated with reproduction, we evaluated Y chromosome abnormalities using a Y chromosome microdeletion detection kit. However, we detected no Yq abnormality as the result of BPA exposure. Secondly, we performed high-density oligonucleotide array-based comparative genome hybridization (CGH) to assess genomic alteration as a component of our toxicity assessment. The results of our data analysis revealed some changes in copy number. Seven observed features were gains or losses in chromosomal DNA (P-value<1.0e-5, average log2 ratio>0.2). Interestingly, 21 probes of chr7:7312289-10272836 (qA1-qA2 in cytoband) were a commonly observed amplification (P-value 3.69e-10). Another region, chr14:4551029-10397399, was also commonly amplified (P-value 2.93e-12, average of log2 ratios in segment>0.3786). These regions include many genes associated with pheromone response, transcription, and signal transduction using ArrayToKegg software. These results help us to understand the molecular mechanisms underlying the reproductive effects induced by BPA.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.1 no.1
• /
• pp.63-69
• /
• 2006

The optical submarine cable has a long distance cable and the repeater for optical amplification compared to territorial optical cable so conventional OTDR utilization for the optical submarine cable is limited. in case the optical core in the optical submarine cable system cut, by using Coherent OTDR that utilize OTDR path in repeater the cable fault point can be detected and in case the faulty of the copper tube in the cable that provide power for the repeater to amplify optical signal, the ways using the current/voltage characteristic, the capacitance per Km and so on is required. this report suggest efficient fault location detecting mechanism by categorized cable fault type.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.8
• /
• pp.4533-4537
• /
• 2013

Objective: To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells. Method: After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs. Results: 70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results. Conclusion: Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2001.05a
• /
• pp.333-336
• /
• 2001

The transient response in PDFA(Praseodymium-Doped Fiber Amplifier) is theoretically investigated. The PDFA has the spectral gain band in 1.3${\mu}{\textrm}{m}$. The transient model includes the transient buildup of the population inversion, the pump power, and the signal power and their transient variation along the fiber amplifier. The numerical analysis of transient model can predict the gain saturation, the variation of pump power and the gain as a function of the fiber length. It also shows the gain saturation and recovery effects depending on the pumping rate lead to distortion and saturation in the amplification of optical pulse. The results of numerical analysis, for the case of the Pr ion concentration of 1000ppm and the pump power of 0.5W the gain saturation is obtained 30dB at the length of 5m and the saturation time of upper level is 250 $mutextrm{s}$.

• Journal of Plant Biotechnology
• /
• v.36 no.4
• /
• pp.407-414
• /
• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Journal of Broadcast Engineering
• /
• v.18 no.6
• /
• pp.884-894
• /
• 2013

In this paper, we investigate the characteristics of power amplifiers and simplified memoryless non-linear power amplifier models for energy efficient communication system. First, we present the transfer function of GaAs FET (Gallium Arsenide Field Effect Transistor) that is widely used for high power amplifier. From those investigations, we introduce the instantaneous efficiencies and methods of amplification by assuming that the saturated current is constant, while perfect linearity is exploited under knee voltage. Then, we discuss four non-linear power amplifier models in a baseband signal processing. Finally, we explain the specified total power consumption model in a base station to achieve the resonable analysis for energy efficient communication.

• KSBB Journal
• /
• v.32 no.1
• /
• pp.71-82
• /
• 2017

To verify anti-diabetic effect of oligopeptide with His-Pro repeats (mHP peptide), the oligopeptide was first secreted and optimized using the secretion vector, pRBAS with alkaline protease gene promoter and the signal sequence in Bacillus subtilis and directly the anti-diabetic effect of the mHP peptide was investigated in insulinoma cell, RINm5F cell line. The oligopeptide gene was obtained by annealing oligonucleotides with repeated His-Pro sequence and finally was constructed as 18 dipeptides (108 bp and 4.0 kDa) coding gene, named oligopeptide with His-Pro repeats (mHP peptide) to make cyclo(His-Pro) known to be anti-diabetic effects. The region encoding the oligopeptide gene was subcloned into the pRBAS secretion vector (E.coli-Bacillus shuttle vector) after PCR amplification using the designed primers including initiation and termination codons and His tag, named pRBAS-mHP (6.56 kb). To optimize secretion of the oligopeptide, various culture conditions were investigated in Bacillus subtilis LKS. As a result, the secreted oligopeptide was maximally measured (approximately $59.6{\mu}g/mL$) in 3 L batch culture and the highest secretion was achieved at $30^{\circ}C$, PY medium, and carbon sources (particularly barley and glycerol). In the RINm5F cells treated with 2 mM STZ, the oligopeptide treatment (0.1 mg/mL) restored the cell viability (10%) and reduced the nitric oxide (NO) generation (35%) and DNA fragmentation (90%). And also, insulin secretion level was increased to 17% higher than in STZ-treated RINm5F cells. These results suggest that the oligopeptide with His-Pro repeats could be a candidate material for anti-diabetic agent against STZ-induced diabetes.

• The Korean Journal of Physiology and Pharmacology
• /
• v.13 no.5
• /
• pp.373-378
• /
• 2009

Cerebellar Purkinje cells (PCs) play a crucial role in motor functions and their progressive degeneration is closely associated with spinocerebellar ataxias. Although immunohistochemical (IHC) analysis can provide a valuable tool for understanding the pathophysiology of PC disorders, the method validation of IHC analysis with cerebellar tissue specimens is unclear. Here we present an optimized and validated IHC method using antibodies to calbindin D28k, a specific PC marker in the cerebellum. To achieve the desired sensitivity, specificity, and reproducibility, we modified IHC analysis procedures for cerebellar tissues. We found that the sensitivity of staining varies depending on the commercial source of primary antibody. In addition, we showed that a biotin-free signal amplification method using a horseradish peroxidase polymer-conjugated secondary antibody increases both the sensitivity and specificity of ICH analysis. Furthermore, we demonstrated that dye filtration using a $0.22\;{\mu}m$ filter eliminates or minimizes nonspecific staining while preserving the analytical sensitivity. These results suggest that our protocol can be adapted for future investigations aiming to understand the pathophysiology of cerebellar PC disorders and to evaluate the efficacy of therapeutic strategies for treating' these diseases.

• International Journal of Advanced Culture Technology
• /
• v.6 no.1
• /
• pp.42-49
• /
• 2018

In this study, we tried to reveal the state of stillness of Cho Ji-Hoon's poem "Silent Night 1" as a healing modifier. The language of poem is synaptically linked to the calmness emotion of the human body, seeking a principle that leads to a state of healing. Therefore, this study was carried out for the purpose of applying the principle to literary therapy program. The silent signal embedded in the poem is encoded into the signals of the sound as it is synapsed to the human body. Encoding of auditory nerves by poem lines is like a Morse code that word and word leave in the human body. The action potential of the auditory nerve is further activated by the potential difference between the word and the word represented by the neural network, such as a Morse code, which is accessed to the human body by such a path. There is worked as amplified potential difference between the words perceived by a sound which is synapsed to the human body and by a silence which is synapsed to the human body. The phenomenon of the words approaching the human body and setting the absence of sound and amplifying the sound is because the words amplifies the Morse codes in the human neural network. At this time, the signals overlap each other. Thereby this poem is increasing the amplitude of the sound. This overlapping of auditory signals appears and amplifies the catharsis. If this Cho Ji-Hoon Poem's principle is applied to literary therapy program in the future, more effective treatment will be done.

• Journal of Power Electronics
• /
• v.16 no.1
• /
• pp.362-373
• /
• 2016

The on-line measurement of high-power IGBT collector current is important for the hierarchical control and short-circuit and overcurrent protection of its driver and the sensorless control of the converter. The conventional on-line measurement methods for IGBT collector current are not suitable for engineering measurement due to their large-size, high-cost, low-efficiency sensors, current transformers or dividers, etc. Based on the gate driver, this paper has proposed a current measuring circuit for IGBT collector current. The circuit is used to conduct non-intervention on-line measurement of IGBT collector current by detecting the voltage drop of the IGBT power emitter and the auxiliary emitter terminals. A theoretical analysis verifies the feasibility of this circuit. The circuit adopts an operational amplifier for impedance isolation to prevent the measuring circuit from affecting the dynamic performance of the IGBT. Due to using the scheme for integration first and amplification afterwards, the difficult problem of achieving high accuracy in the transient-state and on-state measurement of the voltage between the terminals of IGBT power emitter and the auxiliary emitter (u_Ee) has been solved. This is impossible for a conventional detector. On this basis, the adoption of a two-stage operational amplifier can better meet the requirements of high bandwidth measurement under the conditions of a small signal with a large gain. Finally, various experiments have been carried out under the conditions of several typical loads (resistance-inductance load, resistance load and inductance load), different IGBT junction temperatures, soft short-circuits and hard short-circuits for the on-line measurement of IGBT collector current. This is aided by the capacitor voltage which is the integration result of the voltage uEe. The results show that the proposed method of measuring IGBT collector current is feasible and effective.