• Journal of Plant Biotechnology
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• v.50
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• pp.142-154
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• 2023

Plant abscission is a natural process in which plant organs or tissues undergo detachment, a strategy selected by nature for the disposal of nonessential organs and widespread dissemination of seeds and fruits. However, from an agricultural perspective, the abscission of seeds or fruits represents a major factor that reduces crop productivity and product quality. Therefore, during the crop domestication process in traditional agriculture, mutants exhibiting suppressed abscission were selected and crossbred, thereby enabling the production of modern crop varieties such as rice, tomatoes, canola, and soybeans. These crops possess a unique trait of retaining ripe fruits or seeds in contrast to disposal via abscission. During the previous century, research on quantitative trait loci along with genetic and molecular biological studies on Arabidopsis thaliana have elucidated various cell biological mechanisms, signaling pathways, and transcription regulators involved in abscission. Additionally, it has been revealed that various hormone signals, which are involved in plant growth, play crucial roles in modulating abscission activity. Researchers have developed several chemical treatments that target these hormones and signal transduction pathways to enhance crop yields. This review aimed to introduce the previously identified signal transduction pathways and pivotal regulators implicated in abscission activity. Moreover, this review will discuss the future direction of research required to investigate crop abscission mechanisms for their potential application in smart farming and other areas of agriculture, as well as areas within model systems that require extensive research.

• Journal of the Korean Electrochemical Society
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• v.11 no.2
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• pp.105-108
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• 2008

In the recent years, increasing interests are being focused on the rational functionalization of the CNTs by some creative methods. However, the considerable toxicity of CNT is still a controversialissue and limits its biological application. To improve the biocompatibility of CNT, in this work we prepared CNT-$TiO_2$ nanocomposites with CNT and organic titanium precursors. Our observations demonstratethat the modified interface could accelerate the heterogeneous electron transfer rates and thusenhance the relevant detection sensitivity, suggesting its potential application as the new strategy for the development of the biocompatible and multi-signal responsive biosensors for the early diagnosis of cancers.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.11A
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• pp.853-860
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• 2011

In this paper, we proposed a transmission scheme for a full-duplex relay system that minimizes outage probability. A relay system with the full-duplex relay can mitigate resource inefficiency of a half-duplex relay system. However, the mobile station suffers from the interference because the base station and the relay station transmits signal simultaneously to the mobile station. First, we suggest a layered broadcasting for full-duplex relaying. Second, we derive an optimal power allocation for the layered broadcasting in terms of the outage probability minimization. The proposed algorithm shows better performance than a half-duplex relaying system with and without the diversity, and a conventional full-duplex relaying system.

• Journal of IKEEE
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• v.13 no.4
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• pp.75-81
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• 2009

The cellular system in the next generation decreases the range of transmission of a signal as well as increases the rate of transmission adapting the method of multi-hop relaying with the relay. However, the fact of decreasing the range of transmission with the method of multi-hop relaying means increasing interferential amount in the outer cell; therefore, the deployment of the relay can affect to the function of the cellular system. In this thesis, the deployment of the relay is determined for the maximum rate of transmission, based on the transmission power of the relay and the variation of interferential amount. The condition to determine the deployment of the relay is analyzed with the mathematical model; in addition, its performance is verified through the result of a simulation. Based on the analysis of this thesis, the established deployment and transmission power of the relay to minimize the average outage probability exist. Furthermore, the relay contributes to enlargement of capacity of cells, decreasing the average outage probability in the situation of less severe interference between cells with reuse of frequency. However, the relay should be restrained in use in the situation of severe interference between cells due to the fact that the outage probability of inter-cells can be increased.

• Biomolecules & Therapeutics
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• v.24 no.3
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• pp.260-267
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• 2016

Mesenchymal stem cells (MSCs) offer significant therapeutic promise for various regenerative therapies. However, MSC-based therapy for injury exhibits low efficacy due to the pathological environment in target tissues and the differences between in vitro and in vivo conditions. To address this issue, we developed adipose-derived MSC spheroids as a novel delivery method to preserve the stem cell microenvironment. MSC spheroids were generated by suspension culture for 3 days, and their sizes increased in a time-dependent manner. After re-attachment of MSC spheroids to the plastic dish, their adhesion capacity and morphology were not altered. MSC spheroids showed enhanced production of hypoxia-induced angiogenic cytokines such as vascular endothelial growth factor (VEGF), stromal cell derived factor (SDF), and hepatocyte growth factor (HGF). In addition, spheroid culture promoted the preservation of extracellular matrix (ECM) components, such as laminin and fibronectin, in a culture time- and spheroid size-dependent manner. Furthermore, phosphorylation of AKT, a cell survival signal, was significantly higher and the expression of pro-apoptotic molecules, poly (ADP ribose) polymerase-1 (PARP-1) and cleaved caspase-3, was markedly lower in the spheroids than in MSCs in monolayers. In the murine hindlimb ischemia model, transplanted MSC spheroids showed better proliferation than MSCs in monolayer. These findings suggest that MSC spheroids promote MSC bioactivities via secretion of angiogenic cytokines, preservation of ECM components, and regulation of apoptotic signals. Therefore, MSC spheroid-based cell therapy may serve as a simple and effective strategy for regenerative medicine.

• Journal of Korean Society of Transportation
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• v.22 no.2 s.73
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• pp.109-119
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• 2004

In COSMOS, an area for signal coordination is divided into subareas composed of several signalized intersections that share a common cycle time. Each subarea contains only one critical intersection having heavy traffic load. Subarea is a basic unit of control. The performance of COSMOS is highly dependent on the linkage rule between adjacent subareas. The purpose of this study is to provide an appropriate subarea linkage rule in COSMOS. This study developed a control strategy for Critical Intersection and Sub Area linkage. Critical Intersections calculate the Offset Pattern both East-West Axis and North-South Axis, and the coordination direction either East-West Axis or North-South Axis. Subarea can be combined with other one in all directions. The performance of the suggested linkage rule was evaluated on the real network in Gangnam-Gu. The result was that travel time was reduced by the suggested linkage rule.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1169-1175
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• 2009

An antifungal chitinase, ChiCW, produced by Bacillus cereus 28-9 is effective against conidial germination of Botrytis elliptica, the causal agent of lily leaf blight. ChiCW as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-III-like domain, and a chitin-binding domain. When two C-terminal domains of ChiCW were truncated, $ChiCW{\Delta}FC$ (lacking the chitin-binding domain and fibronectin type III-like domain) lost its antifungal activity. Since $ChiCW{\Delta}C$ (lacking the chitin-binding domain) could not be expressed in Escherichia coli as $ChiCW{\Delta}FC$ did, a different strategy based on protein engineering technology was designed to investigate the involvement of the chitin-binding domain of ChiCW ($ChBD_{ChiCW}$) in antifungal activity in this study. Because ChiA1 of Bacillus circulans WL-12 is a modular enzyme with a higher hydrolytic activity than ChiCW but not inhibitory to conidial germination of Bo. elliptica and the similar domain composition of ChiA1 and ChiCW, the C-terminal truncated derivatives of ChiA1 were generated and used to construct chimeric chitinases with $ChBD_{ChiCW}$. When the chitin-binding domain of ChiA1 was replaced with $ChBD_{ChiCW}$, the chimeric chitinase named ChiAAAW exhibited both high enzyme activity and antifungal activity. The results indicate that $ChBD_{ChiCW}$ may play an important role in the antifungal activity of ChiCW.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.305-311
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• 2002

Acetyl xylan esterase gene (AXE) from Aspergillus ficuum was cloned and its Pichia expression plasmid, pPICZ$\alpha$C-AXE (4.6 kb), was constructed, in which the AXE gene was under the control of the AOXI promoter and connected downstream of mating factor u-1 signal sequence. The plasmid linearized by Sacl was integrated into the 5'AOXI region of the chromosomal DNA of P. pastoris. In the flask batch culture of P. pastoris transformant on methanol medium, the cell concentration and total AXEase activity reached at 6.0 g-dry cell weight/1 and 77 unit/ml after 36 h cultivation, respectively. In the fed-batch culture employing the optimized methanol and histidine feeding strategy, the cell concentration and total AXEase activity were significantly increased to about 97 g-dry cell weight/1 and 930 unit/ml. Most of AXEase activity (>90%) was found in the extracellular medium and the majority of extracellular protein (>80%) was AXEase enzyme (33.5 kDa). This result means that about 9.8 g/1 of AXEase protein was produced in the extracellular medium.

• The Transactions of the Korean Institute of Power Electronics
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• v.3 no.3
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• pp.263-272
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• 1998

This paper presents the new control strategy that can minimizes the torque ripple by considering the magnetic nonlinearity and phase torque averlapping intervals, and describes the whole SRM drive system using proposed control method implemented by DSP(Digital Signal Processor). To do this, inductance and torque are, at first, measured according to the variation of rotor position angle while current is kept constant at predetermined several values. From these measured values, the entire inductance and torque for any current and rotor position are inferred by using neural network. And the waveform of the reference phase torque is determined for the torque ripple to be minimized considering the torque overlap between phases. The controller is designed for the actual torque obtained by the inferred torque look-up table using measured current and rotor position angle to track the predetermined reference phase torque by delta modulation technique. To perform a real time processing and ensure the reliability of the controller, DSP is implemented.

• Journal of Power Electronics
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• v.15 no.6
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• pp.1609-1618
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• 2015

DC microgrids are considered as prospective systems because of their easy connection of distributed energy resources (DERs) and electric vehicles (EVs), reduction of conversion loss between dc output sources and loads, lack of reactive power issues, etc. These features make them very suitable for future industrial and commercial buildings' power systems. In addition, the bipolar-type dc system structure is more popular, because it provides two voltage levels for different power converters and loads. To keep voltage balanced in such a dc system, a bidirectional dual buck-boost voltage balancer with direct coupling is introduced based on P-cell and N-cell concepts. This results in greatly enhanced system reliability thanks to no shoot-through problems and lower switching losses with the help of power MOSFETs. In order to increase system efficiency and reliability, a novel burst-mode control strategy is proposed for the dual buck-boost voltage balancer. The basic operating principle, the current relations, and a small-signal model of the voltage balancer are analyzed under the burst-mode control scheme in detail. Finally, simulation experiments are performed and a laboratory unit with a 5kW unbalanced ability is constructed to verify the viability of the bidirectional dual buck-boost voltage balancer under the proposed burst-mode control scheme in low-voltage bipolar-type dc microgrids.