• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.37-44
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• 1991

A shuttle promoter-probe vector, pEB203, was derived from pBR322, pPL703 and pUB110. Using the vector, a useful DNA fragment, 319 bp EcoRI fragment, having strong promoter activity has been cloned from Bacillus subtills chromosomal DNA. Selection was based on chloramphenicol resistance which is dependent upon the introduction of DNA fragments allowing expression of a chloramphenicol acetyl transferase gene. The nucleotide sequence of the 319 bp fragment has been determined and the putative -35 and -10 region, ribosome binding site, and ATG initiation codon were observed. This promoter was named EB promoter and the resultant plasmid which can be used as an expression vector was named pEBP313. The crystal protein gene from B. thuringiensis subsp. kurstaki HD-73 was cloned downstream from the EB promoter without its own promoter. When the resultant plasmid, pBT313, was introduced into Escherichia coli and B. subtilis, efficient synthesis of crystal protein was observed in both cells, and the cp gene expression in B. subtilis begins early in the vegetative phase. The cell extracts from both clones were toxic to Hyphantria cunea larvae.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.6 no.3
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• pp.47-52
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• 2007

LMTT (Linear Motor based Transfer Technology), which is a new type transfer system used in the maritime container terminal for the port automation, is driven by PM LSM (Permanent Magnetic Linear Synchronous Motor), and largely consists of a controller, shuttle car, and rail. The shuttle car is divided into the frame part, the driving part, and wheels. Because the shuttle car is supported by four wheels in opposition to have five times length for the width of it, a larger deflection than other transfer system using the linear motor occurs. This deflection changes the gap between the mover and the stator, and then brings on an ununiform thrust force. So in this study, we dealt with the structural design for the mover of the shuttle car to generate the uniform thrust force for the efficient control of it. For the investigation, the thickness for each beam of the mover was adopted as design variables, the weight of the mover as objective function, and stress and deflection of the mover as constraint condition.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.850-855
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• 2013

Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.

• Food Science and Biotechnology
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• v.18 no.2
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• pp.396-401
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• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

• Journal of the Korea Safety Management & Science
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• v.10 no.4
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• pp.199-207
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• 2008

Many logistics enterprises have made efforts to achieve low costly and high efficient logistics network. The cross-docking system can be a good solution for them. However, it requires tight schedule and all-night operation inevitably for realization of ideal cross-docking. These causes the difficulty of the attainment of daily delivery target and the leave of delivery service persons. In this paper, we develop the line-haul and shuttle service compromised cross-docking model in order to solve the problems practically. We apply the storage process with the cross-docking system and the direct cross-docking between line-haul and shuttle services. The simulation model validates the shorter delivery time by the developed model than the present model.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.321-324
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• 2006

LMTT(Linear Motor based Transfer Technology) is a new type of transfer system used in the maritime container terminal for the port automation, and largely consists of a controller, shuttle car, and rail. The shuttle car is divided into the frame part, the driving part, and wheels. In order to design this system, various researches on each part of it must be conducted. In this study, we dealt with the optimum design for the mover of the shuttle car designed from previous studies on the strength of the frame with respect to the number of cross beams to minimize the weight of the shuttle car and to satisfy design criteria of cargo-handling systems in container terminal. For the optimization of the mover, thicknesses of each beam were adopted as design variables, the weight of the frame as objective function, and stress and deflection per unit length as constraint condition.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.1
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• pp.89-97
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• 2001

A microgyroscope, which vibrates in two orthogonal axes on the substrate plane, is designed and fabricated. The shuttle mass of the vibrating gyroscope consists of two parts. The one is outer shuttle mass which vibrates in driving mode guided by four folded springs attached to anchors. And the other is inner shuttle mass which vibrates in driving mode as the outer frame does and also can vibrate in sensing mode guided by four folded springs attached to the outer shuttle mass. Due to the directions of vibrating mode, it is possible to fabricate the gyroscope with simplified process by using polysilicon on insulator structure. Fabrication processes of the microgyroscope are composed of anisotropic silicon etching by RIE, gas-phase etching (GPE) of the buried sacrificial oxide layer, metal electrode formation. An eletromechanical model of the vibrating microgyroscope was modeled and bandwidth characteristics of the gyroscope operates at DC 4V and AC 0.1V in a vacuum chamber of 100mtorr. The detection circuit consists of a discrete sense amplifier and a noise canceling circuit. Using the evaluated electromechanical model, an operating condition for high performance of the gyroscope is obtained.

• Journal of the Korean Society of Propulsion Engineers
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• v.23 no.4
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• pp.50-60
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• 2019

This paper introduces the development of a toolbox for the Space Shuttle Main Engine(SSME) based on MATLAB/Simulink. A mathematical model of rocket engine creation and validation can be a complex process, the development of a rocket engine toolbox simplifies this process, thereby facilitating engine performance optimization as well as new design development. The mathematical modeling of the SSME dealt with in this paper is formed by 32 first-order differential equations derived from seven governing equations. We develop the toolbox for the SSME classifying each module according to the engine components. Further, we confirm the validity of the toolbox by comparing the results of the simulation obtained using the toolbox with those obtained using the original simulation of the engine.

• Journal of the Korea Convergence Society
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• v.10 no.4
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• pp.195-201
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• 2019

The purpose of this study was to propose an effective field test for assessing the cardiovascular endurance of soccer players by comparing the shuttle run test and the Yo-Yo intermittent recovery test (Yo-Yo test), which are representative of the aerobic exercise performance test, with the VO2max and other sprint tests. The shuttle run test requires the subject to run continuously without a break interval, while the Yo-Yo test has a break interval, so that they run with the recovery period. Therefore, the Yo-Yo test can be used as an effective field test for evaluating the cardiopulmonary function considering the characteristics of the soccer players of intermittent training.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.18 no.4
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• pp.62-68
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• 2019

The aim of this study was to interpret the structure and dynamics of a transfer shuttle system as a material supply device for small machine tools. The following conclusions were obtained by performing a structural interpretation of the material supply equipment with respect to workload and the dynamical interpretation of a flexible multibody carriage shuttle. When a 1,000-kg workload was applied to a fork lift, the safety factor was approximately 1.86. To conservatively assess the integrity of the structure, a 1,000-kg workload would be proper. In the case of a deflection of the fork system, the width increased with increasing time. The greatest deflection occurred at 5.5 s, which was the largest increase in the time point of the fork system.