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Selection of Surrogates and Analysis of Its Ovulation Status for the Production of Somatic Cell Cloned Piglets (체세포 복제돼지 생산에 있어서 대리모의 선발과 배란상태 분석)

  • Hyun Sang-Hwan;Jeung Yeon-Woo;Lee Eun-Song;Kim Hyun-Wook;Kim Gon-Hyung;Jeung Eui-Bae
    • Journal of Veterinary Clinics
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    • v.23 no.2
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    • pp.123-128
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    • 2006
  • Production of cloned pigs by somatic cell nuclear transfer (SCNT) has unlimited value for developing critical biotechnology such as xenotransplantation. Various efforts have been made to establish this technology, and several litters of live piglets have been produced after transfer of SCNT embryos. However, the efficiency is very low compared to piglet production by artificial insemination or natural mating. So far, most studies have been limited to in vitro production of SCNT embryos. This study was conducted to standardize a surrogate recipient (gilts) for transfer of SCNT embryos to improve pregnancy rate. Potential surrogate gilts over 7 months of age were checked for their estrous status by observing external signs; vaginal fluid, vulva redness, vulva swelling, and standing response to back pressure. Viscosity of vaginal fluid was evaluated and classified as none (0), medium (1), and strong (2). Vulva redness and swelling was respectively assessed by none or shrink (0), medium (1), strong (2). Back pressure was estimated by an immediate move (0), standing less than 10 sec (1), and standing over 10 see (2). And then ovulation status of each surrogate was classified as pre-ovulation (PO-17 surrogates), just prior to ovulation (JPO-20 surrogates), in ovulation (IO-12 surrogates), just after ovulation (JAO-14 surrogates) and after ovulation (AO-24 surrogates) at the time of surgery for embryo transfer (ET). Real-time ultrasonographic scanners have been used for pregnancy diagnosis by observing amniotic vesicles. The first pregnancy diagnosis was done on Day 30 after ET and then repeated 2-week interval. In the results, SCNT embryos transferred into JPO surrogates gave better pregnancy rates (45%) than others (4% to 11%) on Day 30 after ET. These result indicates that surrogate gilts in a status just prior to ovulation can offer optimal condition to establish pregnancy by transfer of SCNT pig embryos.

Studies on the Relation between Characteristics of Stem and Leaf Area and Weight in the Mulberry Tree (뽕나무지조의 성상과 엽면적 및 엽중과의 관계에 관한 연구)

  • 김호락;김문협
    • Journal of Sericultural and Entomological Science
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    • v.13 no.1
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    • pp.1-11
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    • 1971
  • This study are conducted to observe the correlation which are concerned with tile internode length to the leaf area and leaf weight. Where as, small leaf variety Gae Ryang Su Ban, and large leaf variety Ro Sang are adopted to observe their correlations in a group consisting of 30 varieties and in identifiable varieties. The results are as follows: A. Such positive correlations are observed between each characteristics and leaf area with leaf weight. (1) There are highly positive correlation between internode length and leaf area with leaf weight together within varieties group. (2) No correlation with stem length is observed, but high correlation is acknowledged in identifiable varieties although positive correlations are recognized. (3) Correlations are showed between stem volume and leaf area with leaf weight in identifiable varieties, especially Gae Ryang Su Ban higher than any other varieties. (4) Petiole length is not correlated to leaf area and leaf weight in varieties group, but petiole weight is highly correlated to leaf area and leaf weight in varieties group, Gae Ryang Su Ban and Ro Sang variety. B. Internode length along with the stem parts shows the same tendency of variance as most varieties do; internode length of I part at the base is the longest one, gradually shrunk to that of III part and gloved longer, and again internode length becomes shorter to the top. C. Variance of leaf area and leaf weight along with the parts are the lowest ones at the I part, gradually increased to the maximum at IV part, and here after it comes to shrink.

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'Study on Oui-Ga-Sil( 胃家實 )' (위가실(胃家實)에 관(關)한 연구(硏究))

  • Han, Gyu-Eon;Ryu, Bong-Ha;Park, Dong-Won;Ryu, Gi-Won;Jang, In-Gyu
    • The Journal of Internal Korean Medicine
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    • v.10 no.1
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    • pp.65-80
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    • 1989
  • About Oui-Ga-Sil(胃家實) in order to considerate the contents recorded in Nai-Gyung Sang-Han-Lon and latter literature, definition, etiopathology, syndrome, differential diagnosis, therapy, Prognosis and prevention were classified. And the results were as follows: 1. Oui-Ga(胃家)was a term which indicated the whole digestive system such as stomach, small intestine, large intestine, rectum and anus. Sil(實)could be defined as the peculiar concept pertaining to the acute and last stage which was invaded to inside bowels because of abundance with evil influence. 2. Eliology of Oui-Ga-Sil was abunt gastric fever originally, injured mucus because of mistreatment, the invasion to inside of outside evil influence through meridian. Pathology was the opening and shutting appearance of gastric abundance with intestinal emptiness, and intestinal abundance with gastric emptiness, Oui-Ga-Sil could be occurred because of gastric abundant dryness and splenic humidifying capacity decrease. 3. Symptom of Oui-Ga-Sil was classified as for the sunlight outside syndrome and the inside abundant syndrome. The sunlight outside syndrome was body fever, sweating, no chilling, on the contrary hatred of fever. The chief complaint of inside abundant syndrome was daily fever, talking in delirium, hand and foot sweating, abdominal distention, difficult defection and those could be pertained to sunlight bowel syndrome. 4. Diagnostic views of Oui-Ga-Sil were that pulse was descending abundant large strong and smooth quick, a coated tongue was yellow, deep yellow, old yellow, thick, scorching dry rough or gray black. On abdominal diagnosis, pressing by hand, patient was conscious of pain, excessive pain, rejection against press, impossible press or intermittent abdominal pain and bowel cutting pain without press. 5. Differential diagnosis was that the sunlight of Nai-Gyung-Fever-Theory was outside desease making meridian the prime object, Baik-Ho-Tang syndrome was making figureless abundant fever the pivotal point. And important differential standard of splenic shrink syndrome was that a daily fever, an irritation with fever were not occurred. 6. Theory of Oui-Ga-Sil was that Seng-Gi-Tang classes had been used in attacking downward or making balance, and moxibustion on Jung-Wan, honey boiling induction theory had been also used. Attacking downward therapy was invigorating method to preserve mucus, and if mucus had been exausted with complicating emptiness prognosis had been appeared badly. 7. Preventing from Oui-Ga-Sil diet by rule, fitness to cold and warmth may be needed to prevent outside evil influence attack and inside evil influence occurrence. Prudence with being very busy, fatigue, wine and woman may be also needed not to be an injury to splanic and gastric spirit.

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Comparative analysis of two methods of laser induced boron isotopes separation

  • K.A., Lyakhov;Lee, H.J.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.407-408
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    • 2011
  • Natural boron consists of two stable isotopes 10B and 11B with natural abundance of 18.8 atom percent of 10B and 81.2 atom percent of 11B. The thermal neutron absorption cross-section for 10B and 11B are 3837 barn and 0.005 barn respectively. 10B enriched specific compounds are used for control rods and as a reactor coolant additives. In this work 2 methods for boron enrichment were analysed: 1) Gas irradiation in static conditions. Dissociation occurs due to multiphoton absorption by specific isotopes in appropriately tuned laser field. IR shifted laser pulses are usually used in combination with increasing the laser intensity also improves selectivity up to some degree. In order to prevent recombination of dissociated molecules BCl3 is mixed with H2S 2) SILARC method. Advantages of this method: a) Gas cooling is helpful to split and shrink boron isotopes absorption bands. In order to achieve better selectivity BCl3 gas has to be substantially rarefied (~0.01%-5%) in mixture with carrier gas. b) Laser intensity is lower than in the first method. Some preliminary calculations of dissociation and recombination with carrier gas molecules energetics for both methods will be demonstrated Boron separation in SILARC method can be represented as multistage process: 1) Mixture of BCl3 with carrier gas is putted in reservoir 2) Gas overcooling due to expansion through Laval nozzle 3) IR multiphoton absorption by gas irradiated by specifically tuned laser field with subsequent gradual gas condensation in outlet chamber It is planned to develop software which includes these stages. This software will rely on the following available software based on quantum molecular dynamics in external quantized field: 1) WavePacket: Each particle is treated semiclassicaly based on Wigner transform method 2) Turbomole: It is based on local density methods like density of functional methods (DFT) and its improvement- coupled clusters approach (CC) to take into account quantum correlation. These models will be used to extract information concerning kinetic coefficients, and their dependence on applied external field. Information on radiative corrections to equation of state induced by laser field which take into account possible phase transition (or crossover?) can be also revealed. This mixed phase equation of state with quantum corrections will be further used in hydrodynamical simulations. Moreover results of these hydrodynamical simulations can be compared with results of CFD calculations. The first reasonable question to ask before starting the CFD simulations is whether turbulent effects are significant or not, and how to model turbulence? The questions of laser beam parameters and outlet chamber geometry which are most optimal to make all gas volume irradiated is also discussed. Relationship between enrichment factor and stagnation pressure and temperature based on experimental data is also reported.

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Sintering Mixtures in the Stage of Establishing Chemical Equilibrium

  • Savitskii, A.P.
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 1999.04a
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    • pp.5-5
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    • 1999
  • The Principal deficiency of the existing notion about the sintering-mixtures consists in the fact that almost no attention is focused on the Phenomenon of alloy formation during sintering, its connection with dimensional changes of powder bodies, and no correct ideas on the driving force for the sintering process in the stage of establishing chemical equilibrium in a system are available as well. Another disadvantage of the classical sintering theory is an erroneous conception on the dissolution mechanism of solid in liquid. The two-particle model widely used in the literature to describe the sintering phenomenon in solid state disregards the nature of the neighbouring surrounding particles, the presence of pores between them, and the rise of so called arch effect. In this presentation, new basic scientific principles of the driving forces for the sintering process of a two-component powder body, of a diffusion mechanism of the interaction between solid and liquid phases, of stresses and deformation arising in the diffusion zone have been developed. The major driving force for sintering the mixture from components capable of forming solid solutions and intermetallic compounds is attributed to the alloy formation rather than the reduction of the free surface area until the chemical equilibrium is achieved in a system. The lecture considers a multiparticle model of the mixed powder-body and the nature of its volume changes during solid-state and liquid-phase sintering. It explains the discovered S-and V-type concentration dependencies of the change in the compact volume during solid-state sintering. It is supposed in the literature that the dissolution of solid in liquid is realised due to the removal of atoms from the surface of the solid phase into the melt and then their diffusicn transfer from the solid-liquid interface into the bulk of liquid. It has been shown in our experimental studies that the mechanism of the interaction between two components, one of them being liquid, consist in diffusion of the solvent atoms from the liquid into the solid phase until the concentration of solid solutions or an intermetallic compound in the surface layer enables them to pass into the liquid by means of melting. The lecture discusses peculimities of liquid phase formation in systems with intermediate compounds and the role of the liquid phase in bringing about the exothermic effect. At the frist stage of liquid phase sintering the diffusion of atoms from the melt into the solid causes the powder body to grow. At the second stage the diminution of particles in size as a result of their dissolution in the liquid draws their centres closer to each other and makes the compact to shrink Analytical equations were derived to describe quantitatively the porosity and volume changes of compacts as a result of alloy formation during liquid phase sinteIing. Selection criteria for an additive, its concentration and the temperature regime of sintering to control the density the structure of sintered alloys are given.

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Revisioned Labour Law and Labour Management Strategy in Hospital (개정노동법과 종합병원의 노무관리전략)

  • Moon, Young-Jeon;Shon, Tae-Yong
    • Korea Journal of Hospital Management
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    • v.15 no.4
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    • pp.191-222
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    • 2010
  • Revisioned labour law was confirmed by vote of National Assembly on 1st January 2010. Under revisions labour law the necessity was expressed to reconstruct and change the relationship between labour and management in hospital. This study was described to investigate the operational statement of labour union in general hospital and to investigate how the labour policy would be change and which form would be appear in labour union in hospital. The survey results which was operated targeting labour managers in hospital were as follows ; first, in the possibility of plural labour unions establishment, university hospital labour managers forecasted the possibility as high. but non-university hospital labour managers forecasted the possibility as low. second, even though the plural union labours would be established in hospitals there would be no difficulties in collective bargaining between labour and management. third, the labour union activities would not be shrink even though there would be wage pay prohibition for the full-time union laboures. As the wage pay prohibition acted on the full-time union labours, countermeasures are required as follows; first, there is a possibility that there will be diversity in the form of full-time union labours. It is need to make the criterion of hospital about the limit of full-time labour union members. It is need to make the criterion of labour activities of non-working hour exception. It is need to cope with the diverse demands of labour union which aim to solve the financial burdens. As the plural labour unions are permitted it is need to make countermeasures as follows ; To analyze whether there is a possibility by the establishment of new labour to be plural labour unions. To establish the countermeasures in short-term aspect to make the countermeasures to cope with the establishment of plural labour unions. In long-term aspect to establish labour and management strategies. Henceforth, found on the revisions labour law, it is need to provide in hospitals as follows; first, it is need to prepare labour management policies and substitutes which suitable for plural labour union period. Second, it is need to determine concretely on the related matters of the union labours and to develop the conclusion guidelines which preparing infective criteria. Third, to examine the effects on the hospital organization by organizational forms of labour union which is weak in financial aspect.

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A STUDY ON THE PHYSICAL PROPERTIES OF RESTORATIVE MATERIALS FOR PHOTO-POLYMERIZATION OF ARGON LASER (아르곤 레이저를 이용한 광중합 수복재의 물리적 성질에 관한 연구)

  • Ju, Sang-Ho;Choi, Hyung-Jun;Kim, Seong-Oh;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.2
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    • pp.368-382
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    • 1998
  • The purpose of this study is to evaluate and compare the results of argon laser for 5 seconds, argon laser for 10 seconds, and visible light for 40 seconds photo-polymerization in compressive strength, microhardness, curing depth, temperature rising during polymerization, and polymerization shrinkage. Hybrid type composite resin(Z-100) and compomer(Dyract) were used to be compared. The compressive strength was measured by an Instron(1mm/min cross head speed) in 60 specimens and the microhardness of the surface was expressed by Vickers Hardness Number(VHN) in 30 specimens. The curing depth was evaluated comparing the different values of upper and lower VHN according to irradiation time and thickness for the light source polymerization in 60 specimens. The temperature rising during photopolymerization was observed by the temperature change with thermocouple sensitizer beneath 40 specimens at the argon laser for 10 seconds and visible light 40 seconds irradiation. The polymerization shinkage was evaluated by calculating the decrease of % volume by using a dilatometer in 30 specimens. The results were as follows ; 1. In the case of compressive strength, the argon laser polymerization groups were higher than visible light group in Z-100 (p<0.05). In Dyract, the argon laser 5 seconds group did not show a significant difference with the visible light 40 seconds group. The argon laser 10 seconds group showed the markedly low value when compared with other groups (p<0.05) 2. In microhardness, Z-100 was better than Dyract when comparing by VHNs (p<0.05); however, there was not a significant difference between two materials in the visible light 40 seconds group and the argon laser 10 seconds group. 3. In the study of curing depth, Z-100 showed the consistent polymerization in argon laser irradiation because there was no difference in the VHN decrease according to the thickness change. Over the thickness control, the results did not show a significant difference between visible light and argon laser group in Z-100; however, in the case of Dyract, the visible light 40 seconds group was better than the argon laser groups(p<0.05). 4. There was a significant difference between the two materials in temperature rising during polymerization (p<0.05), but not a significant difference between irradiation times, 5. There was not a significant difference between the two materials in polymerization shrink age. The argon laser 5 seconds group was smaller than the other groups (p<0.05). It could be concluded that Z-100 polymerization was recommended to use the argon laser for reduction of the irradiation time while Dyract was recommended to use the visible light polymerization.

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Physico-chemical Changes of Radish Cubes for Kakdugi during Salting (간절임중 깍뚜기용 무우 Cube의 이화학적인 변화)

  • Kim, Joong-Man;Shin, Mi-Kyung;Hwang, Ho-Sun
    • Korean Journal of Food Science and Technology
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    • v.21 no.2
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    • pp.300-306
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    • 1989
  • Physico-chemical changes caused by salting Korean radish cubes (for Kakdugi) with sodium chloride solution were investigated. Two-centimter cubes of Korean radish were soaked in saline solution of 5, 10, 15, 20 and 25 percent concentration. Optimum salinity, 3% as determined by taste, was reached in six hours at 5% strength, two hours in 10%, one hour at 15% and within one hour at concentration of 15% plus. Radish cubes salted in 5, 10. 15, 20 and 25% sodium chloride solution in a cube/solution weight ratio of 1:1 decreased in volume from 7.6 to 11.2% after one hour, and from 11.2 to 17.9% after six hours. Decrease in moisture content was from 83.0 to 75.9% in one hour and from 74.5 to 68.5% after six hours. $Potassium\;ion(K^+)$, $calcium\;ion(Ca^{2+})$ and $magnesium\;ion(Mg^{2+})$ content was significantly decreased by salting, but $sodium\;ion(Na^+)$ content greatly increased. In addition, salting caused firmness of the cubes to decrease, and cell shapes to shrink by plasmolysis.

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Spatiotemporal change in ectomycorrhizal structure between Tricholoma matsutake and Pinus densiflora symbiosis (송이와 소나무간의 공생관계(共生關係)에서 외생균근(外生菌根)의 시(時)-공간적(空間的) 구조변화(構造變化))

  • Koo, Chang-Duck;Kim, Je-Su;Park, Jae-In;Ka, Kang-Hyeon
    • Journal of Korean Society of Forest Science
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    • v.89 no.3
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    • pp.389-396
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    • 2000
  • To determine whether the Tricholoma matsutake (pine mushroom, Songyi) is symbiotic or parasitic to Pinus densiflora, structural change in their natural ectomycorrhizas were examined. The mycorrhizal samples were collected at three progressional points in the natural hypogeous colony(shiro) : colony front edge, near the fruiting point and 20cm back. The fine roots in the colonies were typical ectomycorrhizas with fungal mantle and Hartig net. However, the T. matsutake mycorrhizas had unique characteristics compared to other types of ectomycorrhizas. That is, spatially the fungal mantle and Hartig net of the T. matsutake mycorrhizas continued to develop along the growing tip, while temporally those structures declined to shrink changing to black brown in the older part of the roots behind the actively growing tip portion. However, there was no mark that the fungal hyphae penetrated into either the cortical cells, endodermal cell layers or stele. The apical tips of the blackened roots remained alive to form new mycorrhizas with other fungi later. Therefore, we conclude that the mycorrhiza of T. matsutake+P. densiflora is rather a dynamic symbiosis that changes its position spatiotemporally as the root grows than either a simple parasitism or symbiosis.

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Development and Field Installation of a System of Simultaneously Removing Dust and Volatile Organic Compounds from Furan Process in Foundry (주물공장의 Furan 공정에서 발생하는 휘발성 유기 화합물 및 분진의 동시제거 시스템 개발 및 현장설치 연구)

  • Park, Jin Soo;Jung, Jae Hak;Lee, Tae-Jin
    • Korean Chemical Engineering Research
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    • v.44 no.2
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    • pp.136-148
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    • 2006
  • A foundry makes various machinery parts made by iron. For manufacturing machinery parts, they usually uses wooden mold with molding sand and pour the molten iron into wooden mold through inlet. A foundry have many processes including Furan process, In Furan process workers prepares a wooden mold in the molding sand. So they fixes wooden mold in sand housing and then they fill the molding sand in the sand housing. Molding sand should be sticky enough to sustain the shape of wooden mold, so several materials are needed to prepare the suitable molding sand. The first step of Furan process is making the molding sand with molding sand and Voltaic Organic Compounds (VOC) and the second step of Furan process is pour the molding sand into the wooden molding housing. This two step of process generated noxious VOC and various size of dust. So the process is very dirty and dangerous one. Because of these, Workers frequently shrink out of the plant. The company related with foundry usually faced on the difficult situation for engagement and always have shortage of hiring problem. Through this study, we developed a system which removes toxic VOC and dust simultaneously. We design and construct real system and install it at real plant. Before setting up this system, the working surroundings VOC (for formaldehyde) 15 ppm and Dust(for $PM_{10}$) $8,000{\mu}g/m^3$. After setting up this system, working surroundings is improved by VOC (for formaldehyde) 0 ppm, Dust(for $PM_{10}$) $4{\mu}g/m^3$, and the work evasion factor is removed. So we contribute to solve hiring problem of this company and increasing the productivity also.