• 한국산림과학회지
• /
• 제111권4호
• /
• pp.644-650
• /
• 2022

두릅나무는 두릅나무과에 속하는 낙엽활엽관목으로 전국에서 재배되고 있는 고소득 작목이다. 재배의 효율성 제고 및 소득 증대를 위하여 두릅나무의 새순 수확 후 전정 시기 및 연차별 전정에 따른 생산성을 분석하였다. 두릅나무 새순 수확 직후 6일 뒤부터 2주 간격으로 5회 전정하여 최적 시기를 판단하였다. 또한 전정 효과를 규명하기 위하여 정식 후 1년 차에 지상에서 30 cm 높이로 전정하였다. 정식 후 2년 차부터 전년도 발생한 가지를 10 cm 남기고 전정하였으며, 연차별 새순 및 가지 발생 특성을 비교하였다. 그 결과 새순 수확 종료 후 6일이 지난 뒤 처음 전정한 두릅나무의 가지 수가 다른 시기보다 1.3-2.1배 많았다. 새순 특성 또한 다른 시기보다 1.2-1.7배 우수하였으며, 전정이 빠를수록 품질이 좋은 많은 양의 새순을 수확할 수 있었다. 연차별 두릅나무의 전정을 통하여 정식 후 2년 차부터 고품질의 새순(직경 약 1.5 cm, 길이 약 16 cm)을 생산할 수 있었으며, 새순 수확이 가능한 가지 발생을 유도하여 생산성을 높일 수 있었다. 또한, 연차별 전정을 통하여 새순 수확이 쉬운 150 cm 정도의 수고를 유지 시킬 수 있었다. 그 결과 두릅나무의 전정 작업은 새순 수확 후 6일 전후로 빠른 시기에 시행하는 것이 효율적이다. 또한 전정하게 되면 매년 균일한 품질의 새순을 생산할 수 있고, 본당 수확량을 증가시킬 수 있다.

• Journal of Plant Biotechnology
• /
• 제6권1호
• /
• pp.39-43
• /
• 2004

A successful, efficient system for multiple shoot induction and plant regeneration of soybean (Glycine max) was established. Four soybean genotypes were compared for organogenic responses on various media cultured under light conditions. The adventitious shoots (98%, 2.6 shoots/cotyledon) directly from one-day-old cotyledon after germination induced by the hormone treatment and its efficiency was higher than any other conditions. The optimal medium for the induction of multiple shoots from cotyledon in Pungsannamulkong(shoot formation rate, 98%), Lx 16 (83%) and IIpumgeomjeongkong(63%) was MS medium supplemented with 2 mg/L BAP, but for Alchankong(75%), MS medium supplemented with 1mg/L zeatin and 1mg/L IAA, 3% sucrose, 4% Phytagel. Higher root induction (88%) was observed from the shoots placed on rooting medium (hormone-free MS basal). Plantlets were transferred onto the same medium supplemented with 1% activated charcoal for further development. With this treatment, regenerated plantlets were obtained within 7-8 weeks (shoot induction for 4 weeks, rooting and shoot elongation for 3-4 weeks).

• Journal of Plant Biotechnology
• /
• 제6권1호
• /
• pp.51-54
• /
• 2004

This study was conducted to examine differences in shoot regeneration among chrysanthemum cultivars. Leaf explants of chrysanthemum cultivars 'Sulhwa', 'Puma', 'Geummokseo' and 'Sulpoong' were used. Explants cultured on the medium for 2 weeks formed calli at the cut surfaces. Shoots regenerated on MS basal medium supplemented with various concentration combinations of NAA and BAP. Explants were cultured under cool-white fluorescent lamps with a light intensity of $40\mu{Mm}^{-2}$.$s^{-1}$ for 16 $hday^{-1}$, at $25^{\circ}c$ and 70-80% relative humidity. 'Geummokseo' and 'Sulpoong' were the most responsive cultivars in shoot regeneration. Most effective medium for 'Sulhwa' and 'Puma' was MS basal medium supplemented with 10.0 $\mu{M}$ NAA and 5.0 $\mu{M}$ BAP and for 'Geummokseo' MS supplemented with 10.0$\mu{M}$ NAA and 20.0$\mu{M}$ BAP. Regeneration of multiple shoots was observed on MS basal medium supplemented with 1.0$\mu{M}$ or 10.0 $\mu{M}$ NAA and 5.0$\mu{M}$ BAP. High frequency regeneration of adventitious shoots from leaf explants and efficient induction of root from these regenerated shoots were obtained.

• Journal of Plant Biotechnology
• /
• 제8권1호
• /
• pp.37-41
• /
• 2006

In vitro mass multiplication of Vanilla planifolia was investigated using node as explant. Multiple shoots were developed in MS medium supplemented with $2.0mgl^{-1}$ 6-benzylaminopurine and $1.0mgl^{-1}$ $\alpha$-naphthalene acetic acid. Multiple shoots were maintained for 6-T weeks with regular subculturing at the end of $3^{rd}$ week onto fresh medium. The maximum number of shoots at the rate of 12.8 per node segment was achieved over a period of four weeks. The elongated shoots were separated from the shoot clusters and were transferred onto half strength MS medium supplemented with indole-3-acetic acid ($1.0mgl^{-1}$) over a period of 28 days for induction of roots. The development of roots was observed on $7^{th}$ day of incubation. The in vitro raised plantlets were transferred to poly-cups, covered with polyethylene sheets and maintained under shade net for 25 days for hardening. Finally these plants were transferred to field and recorded that 85 % of tissue cultured plants were survived. From the present study, a simple and efficient micropropagation protocol was developed for Vanilla planifolia using single node segments as explants.

• Plant Biotechnology Reports
• /
• 제4권3호
• /
• pp.229-235
• /
• 2010

In this study, we established an in vitro regeneration system to maximize the recovery of leafy perilla (Perilla frutescens L. Britton) plantlets as part of developing a molecular biotechnology-based metabolic engineering program for this crop plant. Hypocotyl segments including the apical buds were used as explants for the direct production of shoots without an interim callus phase. The number of shoots produced from the apical buds peaked within 3-4 weeks, and the shoots were subsequently cultured on Murashige and Skoog (MS) media supplemented with 2 mg $1^{-1}$ benzylaminopurine (BA). Spontaneous rhizogenesis was observed after 7-10 days of culture on MS media without hormonal additives. The rooted shoots developed into normal plants in soil after hardening on distilled water for 3-4 days. The average plantlet regeneration frequency was higher for the apical buds (64.33%) than for the top (15.66%), middle (4%), and basal (1.33%) segments of the hypocotyls. This regeneration system demonstrates a capacity for high-frequency plantlet recovery and thus should be considered for use in the genetic manipulation of leafy perilla.

• 농업과학연구
• /
• 제6권2호
• /
• pp.119-124
• /
• 1979

$SO_2$가스가 사과 Golden Delicious, 축(祝), 국광(國光), Starking, 홍옥(紅玉)등의 신초(新梢)에 미치는 영향(影響)을 조사(調査)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. $SO_2$ 가스에 대한 저항성(抵抗性)은 Starking이 가장 높았고 국광(國光)이 가장 낮았다. 2. Ethylene 발생량(發生量)은 가시적(可視的)인 피해(被害)가 나타나지 않은 8시간 처리후(處理後)에 가장 많았으며 처리(處理)시간이 길어짐에 따라 현저(顯著)히 감소(減少)하였고 국광(國光)에서 가장 완만(緩慢)한 발생량(發生量)의 감소(減少)를 보였다. 3. Chlorophyll a,b 그리고 a/b ratio는 국광(國光)에서 가장 높은 감소(減少)를 보였고 기타의 품종(品種)들도 감소(減少)하는 경향(傾向)을 보였다.

• The Korean Journal of Ecology
• /
• 제17권1호
• /
• pp.59-67
• /
• 1994

팔당호 연안대에서 수심에 따른 줄(Zizania latifolia) 개체군의 저토 환경, 경엽부의 밀도, 높이 및 현존량을 조사하였다. 저토의 pH, 유기물함량, 총질소량, 유호태 인량 및 치환성 칼륨량은 수심에 따른 유의한 차이가 없었으나, 모래 함량은 수심이 깊을수록 적었다. 줄 경엽부의 밀도는 수심이 깊을수록 경엽부의 키가 높았고 경엽무 하나의 건물량과 단위면적당 현존량이 많았다. 경엽무 높이의 빈도분포는 줄이 생장함에 따라서 J자형과 첨두형으로 변하였으며, 높이의 불균등도는 감소하였는데, 그 변화는 수심이 깊은 곳에서 보다 빨리 일어났다. 따라서 줄은 수심이 깊을수록 경엽부의 밀도가 낮아지고, 그 높이와 현존량이 증가하여 수면 위로 경엽부를 돌출시키는 생장전략을 가지는 것으로 해석된다.

• Journal of Plant Biotechnology
• /
• 제6권2호
• /
• pp.113-118
• /
• 2004

Somatic embryos were formed from calli obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and coty-ledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Mura-shige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L). Somatic embryo induction was lesser with different combinations of auxins while it increased to 100% in internodal segment and cotyledonary leaf calli with 6-benzyladenine (BA) (2mg/L) along with 2,3,5-triiodobenzoic acid (TIBA) (2mg/L). The shoots were induced from somatic embryos raised from root, coty-ledonary leaf and internodal segment calli grown on MS medium containing BA in combination with indole-3-acetic acid (IAA). Maximum of 66.67% cultures formed shoots on MS medium containing BA (1mg/L) in combination with IAA (2mg/L). The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3-butyric acid (IBA) (2mg/L). The plantlets transferred to the field showed 70% survival rate after one year.

• 한국조경학회지
• /
• 제27권4호
• /
• pp.50-58
• /
• 1999

This study was carried out to investigate the growth characteristics and propagation methods of L. obtusiloba as a woody landscape plant. The results are obtained as follows: L. obtusiloba was dioecious shrub and shaped with bush type. Leaves were ovate, obtuse, cordate, 7.05 cm long, 7.20 cm wide, and petiole 2.0 cm long. Flowers of L. obtusiloba were diclinous. Soil acidity ranged from pH 4.06 to 5.53 with the lowest at the Mt. Soo-Ri. Mt. Soo-Ri located near factory district, which was considered to damaged by environmental deteoration. While soil organic matter was highest at Mt. Soo-Ri, inorganic nutrients were low. L. obtusiloba grows in the area with low soil acidity and low content of inorganic. Therefore it seemed to be tolerant to air pollution. L. Obtusiloba was high seed germination rate in the plug box and its shoots were longer than seeding box and softwood cutting of L. obtusiloba showed the rooting rate of 50% at 5,000ppm on June 23. To develop a mass propagation method of Korean native L. obtusiloba through an axillary bud culture as a woody landscape plant, about 2∼3 cm shoots induced from explant were subcultured to new media contained different growth regulators. Shoots multiplied most effectively on a WPM containing 1.0 mg/l BA, producing 5.5 shoots with a shoot length of 2.5 cm per shoot explant.

• 한국약용작물학회지
• /
• 제10권2호
• /
• pp.116-119
• /
• 2002

번행초(Tetragonia tetragonoides)의 잎 절편으로부터 기내(in vitro) 증식을 유도 하였다. 2 mg/L BA와 0.5 mg/L NAA가 조합 처리된 MS 기본배지에서 잎 절편으로부터 직접 기관형성 과정을 통하여 부정아가 형성 되었다. 유기한 부정아는 절취하여 2 mg/L NAA와 0.5 mg/L BA이 첨가된 MS배지로 옮겨 유묘의 대량 증식을 시도하였으며, 배양 3주 후 신초의 지속적인 성장을 위해 무기염의 농도가 두배로 증가된 2MS배지로 옮겨 배양 하였다. 증식된 신초로부터 발근은 식물성장조절물질이 첨가되지 않은 1/2MS와 MS배지 모두에서 이루어 졌다.