• Title/Summary/Keyword: Shoot cutting

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The Production of Adventitious Sprouts by Water-soak and Vegetative Propagation of Plus tree of Ash Species Through Cuttings of Their Sprouts (물푸레나무속(屬) 수형목(秀型木)의 수심(水沈)에 의(依)한 지조맹아(枝條萌芽) 발생력(發生力)과 이를 이용(利用)한 녹지삽목(綠枝揷木) 증식시험(增殖試驗))

  • Han, Sang Don;Hong, Sung Ho;Min, Young Taek;Kim, Young Mo;Kim, Hong Eun
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.148-154
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    • 1994
  • As a vegetative propagation method for ash species, which is a dioecism and a long cycle of fructification, cut-stem was soaked in water to induce adventitious sprouts, and 2-year-old ash seedling was cut in a nursery to induce adventitious sprouts. We obtained the 1,019 adventitious sprouts from branches of 101 plus trees througout the country. The mean ortet-age is 48. There is not a correlation between ortet ages and production of adventitious sprouts. These sprouts were placed in a cutting bed for rooting. Root ability varied with environmental factors of cuttings. The best rooting(87%) resulted from cutting performed in a vinyl-plastic greenhouse. Rooting was better on perlite+peat moss(2 : 1) medium than other media tested. The rooting ability was generally higher in 2-year-old ortet than plus tree ortet. In the root development of cuttings the non-container cuttings was better than container cuttings.

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Studies on the Host Range of Colletotrichum dematium Isolated from Anthracnose of Pepper and Toxic Metabolites Produced by the Pathogen (고추 탄저병균(炭疽病菌) Colletotrichum dematium의 기주범위(寄主範圍) 및 대사독소(代謝毒素)에 관(關)한 연구(硏究))

  • Kang, Hi Wang;Yu, Seung Hun;Park, Jong Seong
    • Korean Journal of Agricultural Science
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    • v.14 no.1
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    • pp.26-37
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    • 1987
  • This studies were conducted to investigate pathgenicity and host range of Colletotrichum dematium isolated from anthracnose of pepper, and phytoxicity of its culture filtrate and the partially purified toxin. The results obtained were as follows. 1. Investigation on the host range of C. dematium has revealed that pepper as well as soybean, tomato, spinach, and beet were highly susceptible, egg plant and water melon were moderately susceptible and stone leek was slightly suceptible, but no symptoms were produced on carrot, tabacco, cucumber and melon. 2. The culture filtrates of C. dematium in Czapeck dox liquid media were toxic to leaves of pepper and caused necrosis and wilting of the plant. The toxicity of culture filtrates was most active at 15 days after fungal growth in Czapeck dox liquid media and the toxin productivity in still culture was higher than that in shaking culture. 3. The partially purified toxic substance was isolated from the culture filtrates by the acetone precipitation method. When cuttings of various pepper cultivars were placed in the toxin solutions, suceptible cultivars and resistant cultivars were equally toxic and showed necrosis and wilting of the leaves. 4. Several other plants such as soybean, tomato and carrot were also affected with the toxin solution by shoot cutting bioassay and showed veinal necrosis, leaf spots and wilting of the shoots. 5. The acetone precipitation toxin affected seed germination of pepper, cucumber, sesame and egg plant and inhibited the growth of root and hypocotyl of the seedlings.

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Rooting and Survival Rate as Affected by Various Types and Concentrations of Auxin on 'Maehyang' Strawberry in Cutting Propagation ('매향' 딸기의 삽목 번식 시 옥신의 종류 및 처리 농도에 따른 발근율과 생존율)

  • Hwang, Hee Sung;Jeong, Hyeon Woo;Lee, Hye Ri;Jo, Hyeon Gyu;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.56-64
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    • 2021
  • This study was conducted to examine the effect by different types and concentrations of auxin on the rooting and growth of strawberry (Fragaria × ananassa Duch. cv. Maehyang) cuttings in the greenhouse. The NAD (1-naphthylacetamide), IBA (indole-3-butyric acid), and IAA (3-indoleacetic acid) were applied with a 1 hour soaking as 50, 100, 150, and 200 mg·L-1, respectively. The non-treatment was set as the control. The cuttings of strawberry were transplanted in the strawberry seedling tray filled with coir medium on June 4, 2020. The humidification was carried out for 2 weeks. The average relative humidity, daytime temperature, and nighttime temperature inside the humidification tunnel was 63.4 ± 15%, 29.3 ± 5℃, and 16.2 ± 5℃, respectively. There was no significant difference in rooting rate on the control, IBA, and IAA treatments. However, significantly low rooting rates were observed in NAD treatments. The survival rates were significantly higher in the control and IBA with 50 mg·L-1 than in other treatments. The number of leaves was the highest in IBA with 100 mg·L-1. The root length was the longest in the control. More number of roots were counted in IAA with 100 and 150 mg·L-1. The dry weight of root was the heaviest in the control. The total root length, root surface, number of root tips, and number of root forks were significantly higher in the control. As a result, control, IAA, and IBA showed similar shoot and root growth. However, NAD showed the worst root and shoot growth. Consequently, compared with IAA and IBA, NAD was not appropriate plant growth regulator of rooting for cutting propagated strawberries.

Sensitivity of Five Clones of Populus alba × P. glandulosa Cuttings to Ozone Exposure in Open-Top Chambers in Relation to Their Growth Rates (Open-Top chamber 내(內)에서 오존에 노출(露出)시킨 현사시 5개(個) 클론의 생장량(生長量)과 오존에 대(對)한 민감성(敏感性)과의 관계(關係))

  • Kim, Tae Kyu;Lee, Kyung Joon;Kim, Goon Bo;Koo, Yong Bon
    • Journal of Korean Society of Forest Science
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    • v.89 no.1
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    • pp.105-115
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    • 2000
  • This study was conducted to test a hypothesis that sensitivity of trees to ozone exposure was related to their growth rates. Two cultivars of Populus alba ${\times}$ P. glandulosa with different genetic growth potential were used for the comparison. Two clones(72-30, 72-16) of cultivar No. 4 with fast growing potential and three clones(71-28, 72-27, 72-19) of cultivar No. 2 with slow growing potential were propagated in early spring by cutting in $2-{\ell}$ plastic pots. They were grown outdoor for 5 months and exposed in late August for 30 days to 70 and 130ppb ozone in a open-top chambers(2.5m in diameter and 2m in height). Ozone concentration in a control chamber was maintained below 30ppb by filtering with activated charcoal. Each treatment was replicated twenty times. In a control chamber, cultivar No. 4 grew 73%, 64%, and 38% faster than cultivar No. 2 in leaf weight, root weight, and total dry weight, respectively. Visible injury was observed only in cultivar No. 4 in 130ppb treatment. Ozone treatment at both 70 and 130ppb decreased height growth, dry weight of leaf, root, and entire plants in all five clones. Particularly root growth was reduced by 39.7% and 13.8% in cultivar No. 4 and No. 2, respectively, in 70ppb treatment. Consequently, shoot/root ratio of cultivar No.4 was increased by 63.4%, while that of cultivar No.2 was increased by 22.1%. Stomatal conductance decreased more in cultivar No.4 than in cultivar No.2. Net photosynthesis of cultivar No.4 at 130ppb ozone decreased by 69.5%, while that of cultivar No.2 decreased by 31.5%. Above mentioned physiological responses of two cultivars to ozone strongly suggested that fast growing cultivar No.4 was more sensitive to ozone than slow growing cultivar No.2. It was concluded that sensitivity of trees to ozone exposure was closely related to their growth rates.

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Optimum Culture Conditions of Sweetpotato Stem Cut for Shoot-transplant Production during Winter (고구마 줄기묘의 원동육묘 적정조건)

  • 안영섭;정병춘;정미남;오용비;송연상;민경수;강윤규
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.6
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    • pp.382-386
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    • 2000
  • This study was conducted to know the optimum conditions for overwinter culture of sweetpotato stems in PE film house. The stems will be used as transplant shoots in the next year instead of sprouts produced from storage roots. Sweetpotato stems were cut at field on harvest season and planted in PE film house under three different conditions of PE film mulching, tunnel, or mulching plus tunnel in comparison with the non-treatment of PE film on October 10 and November 10. The survival rate of sweetpotato stems, which was evaluated on April 10 after overwinter, was higher in the treatment planted on October 10 than that on November 10, and with PE film treatments, it was higher in tunnel or mulching plus tunnel than that of the non-treatment of PE film. The survival rate of sweetpotato stems to planting densities was 95-96% in 10$\times$2cm (333 stems/$m^2$) or in 10$\times$4cm (250 stems/$m^2$) when compared with 10$\times$2cm (500 stems/$m^2$). The survival rate under low temperature showed 95% until 20 days at 5$\pm$1$^{\circ}C$, and 0% within 5 days at 2$\pm$1$^{\circ}C$. From these results, it was concluded that there were optimum conditions that cutting time is middle October, planting density is 10$\times$3cm, and minimum maintenance temperature is 5$^{\circ}C$ in growing conditions of sweetpotato stems. Root yield produced by trans-planting shoots using the stems was similar to yield by shoots produced from roots, and the survival rate was not different among varieties.

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Effect of Planting Density on Growth and Quality in Hydroponics of Sedum sarmentosum (돌나물 수경재배에서 재식밀도에 따른 생육 및 품질 특성)

  • Lee, Seung-Yeob;Kim, Hyo-Jin;Bae, Jong-Hyang
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.580-584
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    • 2010
  • The effect of planting densities on the growth of $Sedum$ $samentosum$ (4 collections) was investigated using nutrient film technique (NFT) with Yamazaki' lettuce nutrient solution (1982) from September 5th to October 24th in non-heating plastic film house. At 40 days, the plant height of 4 collections showed the range of 15.0-18.9 cm, and mean plant height was high the order of $10{\times}10$, $2.5{\times}2.5$, and $5{\times}5cm$. The growth of 'Pohang' and 'Wando' collections was better compared to that of 'Gunsan' and 'Wanju' collections. Number of node, stem diameter, and fresh and dry weight per plant were decreased in higher planting density. Despite the lower fresh weight per plant obtained, the fresh yield per $m^2$ was significantly increased in higher planting density. The mean fresh yield was $14.9kg{\cdot}m^{-2}$ in $2.5{\times}2.5cm$, and 'Pohang' collection showed the highest fresh yield ($17.6kg{\cdot}m^{-2}$). The first optimized harvesting time base on plant height was 30-40 days after NFT culture during autumn season. In eating quality, compression force of stem and bitterness of shoot were decreased in higher planting density. However, the heavy labor demanding high density cutting needs to be improved for hydroponic culture of $S.$ $samentosum$.

Trinexapac-ethyl Treatment for Kentucky Bluegrass of Golf Course during Summer (하절기 켄터키블루그래스 관리를 위한 식물생장조절제 Trinexapac-ethyl의 활용)

  • Tae, Hyun-Sook;Hong, Beom-Seok;Cho, Yong-Sup;Oh, Sang-Hun
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.156-160
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    • 2010
  • This study was performed to provide useful information for kentucky bluegrass management during summer by application of plant growth regulator, Trinexapac-ethyl. Visual quality, shoot density and chlorophyll contents of treatment blocks were significantly different from those of control during summer by application of Trinexapac-ethyl. The turfgrass density of treatment was increased of 4ea/$10\;cm^2$, especially about 5ea/$10\;cm^2$ during the growth retarded period of June and July. Chlorophyll contents index and visual quality of kentucky bluegrass were improved by application of Trinexapac-ethyl during summer, too. In addition, the occurrence of foliage in rainy and high temperature season was less than that of control. However, there was no significant difference in the root length of Kentucky Bluegrass. Meanwhiles, mowing height of kentucky Bluegrass was suppressed by 38% at 2 WAT week after treatment and that there was no significant growth of treatment at 4 WAT. In this experiment, turfgrass quality was significantly better than that of control during July, even though trinexapac-ethyl wasn't applied at all in July. Consequently, periodic treatment of trinexapac- ethyl from spring would be very important to promote the turfgrass visual quality during summer which is unfavorable season on the growth of kentucky bluegrass. And it is possible to reduce mowing times at least 30% for 2 weeks by one application of Trinexapac-ethyl 0.02~0.03 ml/$m^2$ in kentucky bluegrass fairway. Additively, trinexapac- ethyl treatment can be helpful environmentally by cutting down the fertilizers and pesticides in golf course.

On the rooting of the cuttings of two conifer species (송백류삽수(松柏類揷穗)의 발근(發根)에 관(關)한 연구(硏究))

  • Yim, Kyong Bin
    • Journal of Korean Society of Forest Science
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    • v.1 no.1
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    • pp.12-21
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    • 1962
  • Outdoor cuttings studies on two coniferous species, pitch pine and needle fir, have been made at Suwon, Korea extending from March to early May, 1956. The purpose of these studies was to find the effects of ages of parent trees, topophysis, dilute hormone solution treatments and the time at which the cuttings were collected on the rooting response of the cuttings. On September 2, rooting results were examined. Pitch Pine (Pinus rigida) The total of 1335 cuttings were collected from 3- and 10-year old trees. The cuttings treated were planted on March 13 and April 20. 1. In the cuttings collected from 3-year-old seedlings, the best result (34 percent rooted) was obtained by treating ends of cuttings with a mixture of 50 ppm IBA and 50 ppm NAA. 2. No rooting was observed in lammas shoots. 3. The cuttings from 3-year-old seedlings gave better rooting than those from 10-year-old trees. 4. The root origins were developed from the lower part of the cutting and the cut surface. Needle fir (Abies holophylla) One hundred twenty-five cuttings were collected from a 30-year-old tree. The cuttings treated with any one of four mixed hormone solutions were planted on March 23. 1. Fifteen cuttings out of 75 cuttings from the lower half of the crown were rooted. The cuttings obtained from the upper half of the crown did not root at all. 2. The cuttings treated by the hormone solution made up of 20 ppm IAA, 20 ppm IBA, 20 ppm NAA, 20 ppm thiamine and 5 percent sucrose gave the best rooting (22 percent). 3. All roots originated from callus tissues and irregularly arranged parenchymatous tissues near the cut base.

Effects of Excising In Vitro-Formed Roots on Acclimatization of Micropropagated Cassava Plantlets (카사바의 미세증식에서 기내 발생 부정근의 절단이 순화에 미치는 영향)

  • Yoon, Sil;Cho, Duck-Yee;Soh, Woong Young
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.2
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    • pp.103-108
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    • 2001
  • The in vitro plantlets of cassava (Manihot esculenta Crantz cv. MColl 22) could be regenerated from nodal explant cultures in a liquid MS basal medium containing 0.01 mg/L zeatin for 2 weeks. The plantlets of 1.5∼2.5 cm in shoot length were transplanted to a glass bottle containing fine sand and acclimated under non-sterile conditions after excising their intact roots by: 1) prune leaving roots base of 1∼1.5 cm; 2) complete removal of roots; and 3) cutting off the rooting zone. The majority of in vitro-formed intact roots continued growth after transferred to soil, and all of the damaged roots stopped further growth. The plantlets with excised roots began to develop new roots within 7∼10 days after being transferred to a glass bottle, and a few of the pruned roots developed lateral roots from the remaining portion. Pruning and removal of in vitro roots resulted in a high survival rate (>87%), and did not significantly affect ex vitro root regeneration and acclimation, but the plantlets in which the rooting zone had been cut-off showed 73% survival rate. Pruning or removal of in vitro roots before transfer of plantlets is recommended for useful method of commercial micropropagation because of easier handling and high survival rate of plantlets.

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Effect of Silicate Supplemented to Medium on Rooting of Cutting and Growth of Chrysanthemum (배지에 첨가한 규산질 비료가 국화의 삽수 발근과 생육에 미치는 영향)

  • Moon, Ho Hwan;Bae, Min Ji;Jeong, Byoung Ryong
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.107-111
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    • 2008
  • This experiment was carried out to examine the effect of silicate on the rooting of cuttings and growth of cut chrysanthemum. In the first experiment, cuttings of chrysanthemum 'Pink Pixie Time' were grown in a 3 L medium which was supplemented with silicate at 0, 30, 60, or 90 g for examination of its effect on rooting of cuttings. In the second experiment, chrysanthemum 'Backwang' was grown in medium supplemented with silicate at 0, 60, 90, 120 or 240 g per 20 L medium. In the first experiment, the promotional effect of silicate increased with increasing silicate concentration in the medium, resulting in increased length of shoot and root. However, root dry and fresh weights were not affected by silicate concentration in the medium. In the second experiment, number of branches, plant height, number of nodes, and stem diameter in the silicate-supplemented treatments were greater than those in the control. However, dry matter was not affected significantly by silicate concentration in the medium. Fresh weights of the leaf, stem and root in the silicate-supplemented treatments were greater than those in the control.