• Weed & Turfgrass Science
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• v.5 no.4
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• pp.260-267
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• 2016

Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.4
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• pp.400-407
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• 1989

This experiment was carried out to study the effects of nitrogen concentration of cultural solution, Rhizobium inoculation, and planting density on the growth and yield of soybean cultivars, Hwanggeumkong, Jangbaegkong, Paldalkong, Clark, and non-nodulation isoline of Clark. Rhizobium inoculation increased the stem length, particularly in Hwanggeumkong, Jangbaegkong, and decreased it significantly in non-nodulation Clark. Stem length was increased by the increase in nitrogen fertilization by the 195ppm level and decreased by the increase in plant population density. Rhizobium inoculation also increased the shoot dry weight, but significantly decreased it in non-nodulation Clark. As nitrogen concentration in the cultural solution increased the shoot dry weight decreased in Jangbaegkong and paldalkong. However, the shoot dry weight was decreased by the increase in plant population density. Rhizobium inoculation and the increase in nitrogen concentration of cultural solution increased the ratio of shoot dry weight to root weight. The Rhizobium inoculation and the increase in nitrogen concentration of cultural solution increased the grain yield per pot in Hwanggeumkong and paldolkong, While non-nodulating Clark showed significant decrease in grain yield. Grain yield per pot was also increased by the increase of plant population density. Grain yield was significantly correlated with shoot dry weight, nodule number, and nitrogen content of the soybean plants. The correlation between nitrogen contents of the soybean plants and stem length, shoot dry weight, and nodulation was significant. The allantoin-N content in stem was also significantly correlated with nodulation.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.212-217
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• 2021

This experiment was conducted to compare effects of summer pruning at different times and dormant pruning on shoot growth and yield of northern highbush blueberry. Using 7 (2018) to 9 (2020) years old 'Duke' bushes grown in 180 L containers, summer pruning was treated on June 20 (around 30 days after harvest), July 20, and August 20 consecutively in both 2018 and 2019 removing 30% of the total woods, while dormant pruning (conventional) was on January 20 in both 2019 and 2020. Summer pruning reduced shoot growth the following year, especially when treated in late summer. Total shoot length per bush decreased to 47%, 37%, and 33% on October 15, 2020 in June, July, and August pruning, respectively, compared with that of dormant pruning. Summer pruning at different times in 2018 and 2019 did not affect berry characteristic in the following year. Yield per bush was not significantly changed in 2019, but it decreased by 21 to 38% in 2020 in the summer pruning treatments compared with 2.9 kg of the dormant pruning. It was concluded that consecutive summer pruning in 'Duke' under unheated plastic house could weaken the shoot growth with reducing yield.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.334-340
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• 2005

Lectin is a cell-agglutinating and carbohydrate-binding protein present in many plants. The lectin of Canavalia ensiformis shoot with specific affinity for D-glucose was purified by affinity chromatography using Sephadex G-100, and some of its biochemical characterizations were studied. Lectin was purified 8.87-fold and exhibited final specific activity of 225.74 units/mg protein with a $2.3\%$ yield. SDS-PAGE analysis demonstrated that the purified shoot lectin exists as a tetramer of 102 kD, composed of two subunits with molecular weight of 29 and 22 kD. The purified lectin was observed to agglutinate rabbit blood cell. The optimal temperature for the activity of this lectin was $40^{\circ}C$, and this lectin was relatively stable to heat with the highest activity at $50{\~}60^{\circ}C$. The maximal activity was observed at pH 7.2.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.141-148
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• 2023

Black shoot blight disease caused by Erwinia pyrifoliae has serious impacts on quality and yield in pear production in Korea; therefore, rapid and accurate methods for its detection are needed. However, traditional detection methods require a great deal of time and fail to achieve absolute quantification. In the present study, we developed a droplet digital polymerase chain reaction (ddPCR) method for the detection and absolute quantification of E. pyrifoliae using a pair of species-specific primers. The detection range was 10³-10⁷ copies/ml (DNA templates) and cfu/ml (cell culture templates). This new method exhibited good linearity and repeatability and was validated by absolute quantification of E. pyrifoliae DNA copies from samples of artificially inoculated immature pear fruits. Here, we present the first study of ddPCR assay for the detection and quantification of E. pyrifoliae. This method has potential applications in epidemiology and for the early prediction of black shoot blight outbreaks.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.90-96
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• 1991

This experiment was carried out in order to estimate the recovery days of root and stubble to the days after cutting, and contribution of specific stubble weight on the regrowth was examined using the relationships between the dry weight of shoot and yield components, and regrowth parameters by the days after cutting. The varieties examined were Maprima, Manhattan, Tove, Peramo, Caliente, Tempo and P-2 grown under individual plant basis. The results are may be summarized as follows: 1. Dry weight of root and stubble were recovered up to 13.5 and 11 days after cutting, respectively. 2. Dry weight of shoot(regrowth parts+stubble) was affected significantly by the varieties, stages of regrowth and variety x stage of regrowth. 3. The variety with tiller weight type showed higher average productivity of shoot than those of the variety with tiller number type. 4. Absolute growth rate(AGR) of shoot was correlated significantly with regrowth parts, stubble, root and weight of a tiller at the early stage of regrowth(up to 12 days after cutting), and correlated with regrowth parts, stubble, weight of tiller and stubble area at the late stage of regrowth(up to 20 days after cutting). 5. Contribution of specific stubble weight to absolute growth rate of shoot was different between the stages of regrowth. Thus, regrowth parts per specific stubble weight(RP1SSbW) and weight of tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the early stage of regrowth, and efficiency of specific stubble weight(ESSbW), regrowth parts per specific stubble weight (RPISSbW) and weight of a tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the late stage of regrowth. 6. Regrowth utilization rate(RUR) was one of the useful regrowth parameter to indicate the regrowth potential of grasses.

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.13-16
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• 1970

The studies were intended to clarify the correlations between the characters and the influences upon yield of mulberry trees. The analysis of covariance was used to obtain the genotypic correlations and phenotypic correlations among the eight characters namely branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, etc., and path-coefficients were calculated by Dewey's method (5). The results obtained are summarized as follows： 1. Genotypic correlations were slightly higher than the corresponding phenotypic correlations between tile characters as shown in Table 1. It could be indicated that hight leaf yield was genetically correlated with almost all characters studied except branch diameter. 2. Leaf yield of mulberry trees was mainly associated with the characters such as node number per branch, total branch weight and old branch weight, etc. 3. Branch length, branch diameter, branch number per stock and new shoot weight had little direct influence upon yield but it had rather considerable indirect in fluence upon yield by increasing the node number and total branch weight per stock. As a result, it can be concluded that total branch weight is primarily determined by branch number per stock and old branch weight, and in turn the leaf yield of mulberry trees is secondary determined by the node number by increasing the branch diameter and length which are positively correlated with branch number per stock and new shoot weight of mulberry trees.

• Journal of Sericultural and Entomological Science
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• v.19 no.1
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• pp.1-4
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• 1977

This work was carried out to know the relation between the yield and the cutting length of mulberry branches in autumn. The results obtained are summarized as fellows, 1. Most of the experimented mulberry trees grew evenly, of which branches showed 171cm to 177cm in average length. 2. No difference could be seen in autumn yield among the treatments, however, the shorter branches were remained, the higher in ratio of re-sprouting bud. 3. The spring yield of cutting mode in 100cm and 200cm remained was as much as non-treatment, but dropped remarkably in below 80cm remained. The high productivity in the 100cm or above renamed mode accounts for the low ratio of no-sprouting bud and accelerating the growth of the shoot, which consequently become much of shoot yield per 1m of branch. By cutting branches, however, the ratio of amount of leaves per shoot became low. 4. The cutting mode in 100cm and 120cm remained can yield as much as non-treatment even in the total yields of spring and autumn. but 100cm mode seems to be more advanceable in autumn owing to a little sparing labor for picking leaves. Thus, the most desirable cutting mode in autumn may be in 100cm remained, which usually has been done at the part of one third or half of the whole branches.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.161-166
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• 1994

Since garlics (Allium sativum L.) are propagated through cloves, infection by virus or other pathogens may become severe problem if not using high quality seed bulbs every year resulting in the reduction of yield and bulb quality, In order to solve this problem, the establishment of virus-free bulb production and its supply system have been required because no chemicals were found to eliminate viruses from seed bulbs. This experiment was conducted to develop an effective production technique of high quality seed bulbs using shoot-tip culture. Over 90% of shoot-tips explanted on January L 1990 were survived at constant temperature of either 20, 24 or 28$^{\circ}C$, wheres 88% at alternate temperature (28/20$^{\circ}C$). The growth of shoot and root was most vigorous at constant 24$^{\circ}C$, and least at alternate temperature (28/20$^{\circ}C$) condition. When shoot-tips were explanted June 21 to August 1,1991, survival and growth of shoot-tips was most vigorous on MS medium supplymented with 0.1 mg/L NAA and 2 mg/L kinetin and least 1 mg/L Gh$_3$. The shoot-tips taken from the seed bulbs stored at 4$^{\circ}C$ for 15 to 60 days were placed on MS medium, shoot growth and in vitro bulblet formation increased slightly as affected by the increase of told treatment period at 4$^{\circ}C$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.140-145
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• 1995

This experiment was canied out to investigate the effect of seeding times on the fresh yield of fodder beet varieties, and to select the most adaptable varieties of fodder beet in Daekwanryeong areas. Three varieties(Kyros, Zorba and Hugin) and six seeding times(25 July, 4 Aug. and 14 Aug. in 1993. and 30 Apr., 10 May and 20 May in 1994) were combined in the experiment. The results obtained were as follows: 1. The seeding time for the highest fresh yield of fodder beet was obtained as 18.29 tonha on 25 July in 1993. Of various varieties, the highest fresh yields were 22.59 ton and 18.89 tonlha for Kyros and Zorba on 25 July, and 19.15 tonha for Hugin on 4 Aug. in 1993. 2. The fresh yield of fodder beet were 20.30 ton, 19.41 ton and 16.93 tonlha on 30 Apr., 10 May and 20 May in 1994, respectively. The highest fresh yield of the varieties were 77.78 ton for Kyms on 20 May, 19.79 ton for Zorba on 30 Apr., and 20.63 tonha for Hugin on 10 May in 1994. 3. Shoot and root(S/R) ratio was different between seedling times. S/R ratio of the varieties were increased in 1993 due to the delayed seeding times, but on the contrary it tended to decrease in 1994 with the delayed seeding times. 4. Shoot and root(S/R) ratios of varieties were 0.94, 0.89 and 1.18 for Kyros, Zorba and Hugin in 1993, and 1.29 for Kyms, and 1.85 for Zorba and Hugin in 1994, respectively.