• Title/Summary/Keyword: Shock Structure

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Continuous Synthesis of Escherichia coli GroEL at a high Temperature

  • Kwak, Young-Hak;Lee, Kyong-Sun;Kim, Ji-Yeon;Lee, Dong-Seok;Kim, Han-Bok
    • Journal of Microbiology
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    • v.38 no.3
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    • pp.145-149
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    • 2000
  • GroEL is a typical molecular chaperone. GroEL synthesis patterns at various culture temperatures in Escherichia coli were investigated in this study. No significant differences in the amount of GroEL produced from the chromosome were found at 30 and 37$^{\circ}C$. However, GroEL production increased 3.4-fold at 42$^{\circ}C$. GroEL synthesis was not transient but continuous at 42$^{\circ}C$, although most heat shock gene expression is known to be transient. To understand the role of the groEL structural gene, a groE promoter-lacZ fusion was constructed. Interestingly , while transcriptional fusion is not thermally inducible, it is inducible by ethanol, suggesting that the secondary structure of the groEL transcript is involved in thermal regulation of the groEL gene. Secondary structures of groE mRNA at 37 and 42$^{\circ}C$ were compared using the computer program RNAdraw. Distinct structures at the two temperatures were found, and these structures may be related to a high level of GroEL expression at 42$^{\circ}C$.

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Predicting Micro-Thickness of Phase Fronts in Propellants (추진제의 마이크로 스케일 상면 두께 예측)

  • Yoh Jai-Ick
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2005.11a
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    • pp.13-21
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    • 2005
  • I consider the structure of steady wave system which is admitted by the continuum equations for materials that undergo phase transformations with exothermic chemical reaction. In particular, the dynamic phase front structures between liquid and gas phases, and solid and liquid phases are computationally investigated. Based on the one-dimensional continuum shock structure analysis, the present approach can estimate the nano-width of waves that are present in combustion. For illustration purpose, n-heptane is used in the evaporation and condensation analysis and HMX is used in the melting and freezing analysis of energetic materials of interest. On-going effort includes extension of this idea to include broad range of liquid and solid fuels, such as rocket propellants.

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Backbone 1H, 15N, and 13C Resonances Assignment and Secondary Structure Prediction of SAV0506 from Staphylococcus aureus

  • Lee, In Gyun;Lee, Ki-Young;Kim, Ji-Hun;Chae, Susanna;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.17 no.1
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    • pp.54-58
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    • 2013
  • SAV0506 is an 87 residue hypothetical protein from Staphylococcus aureus strain Mu50 and also predicted to have similar function to ribosome associated heat shock protein, Hsp 15. Hsp15 is thought to be involved in the repair mechanism of erroneously produced 50S ribosome subunit. In this report, we present the sequence specific backbone resonance assignment of SAV0506. About 82.5% of all resonances could be assigned unambiguously. By analyzing deviations of the $C{\alpha}$ and $C{\beta}$ chemical shift values, we could predict the secondary structure of SAV0506. This study is an essential step towards the structural characterization of SAV0506.

Numerical Study of Electrohydraulic Forming Using an Arbitrary Lagrange-Eulerian Method (Arbitrary Lagrange-Eulerian 기법을 활용한 액중 방전 성형의 해석적 연구)

  • Woo, M.A.;Noh, H.G.;Song, W.J.;Kang, B.S.;Kim, J.
    • Transactions of Materials Processing
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    • v.25 no.1
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    • pp.49-55
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    • 2016
  • Electrohydraulic forming (EHF) is a high-speed forming process that uses an electric arc discharge in water. Shock waves resulting from the electric arc discharge are propagated to the blank through water and the blank moves toward the die. Advantages of EHF include improved formability due to the high-speed process and reduction of the bouncing effect. In the current study, a numerical simulation of EHF was developed using LS-DYNA. In the simulation, the model for the electric arc was assumed as an adiabatic gas expansion and an Arbitrary Lagrange-Eulerian (ALE) multi material formulation was used to describe the interaction between the electric arc and the water. In order to model the Fluid-Structure Interaction (FSI), a coupling mechanism was used. The blank of Al 1100-O was simulated using shell elements. The results of the simulation showed that the blank was deformed due to the pressure propagation of water and the bouncing effect did not affect the formability of blank.

The Electrical Characterization of the Quantized Hall Device with GaAs/AlGaAs heterojunction structure (GaAs/AlGaAs 이종접합된 양자흘 소자의 전기적 특성)

  • 유광민;류제천;한권수;서경철;임국형
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07a
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    • pp.334-337
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    • 2002
  • The Quantum Hall Resistance(QHR) device which consists of GaAs/AlGaAs heterojunction structure is used for the realization of QHR Standard based on QHE. In order to characterize electrical contact resistances and dissipations of the device, it is slowly cooled down for eliminating thermal shock and unwanted noise. Then, the two properties are measured under 1.5 K and 5.15 T. Contact resistances are all within 1.2 Ω and longitudinal resistivities are all within 1 mΩ up to DC 90${\mu}$A. The results mean the device is operated well to realize the QHR Standard. To confirm it, the QHR Standard having the device is compared using a direct current comparator bridge with a 1 Ω resistance standard which the calibrated value is known from QHR standards maintained by other countries. The difference between them is agreed well within measurement uncertainty. It is thus considered that the properties of the device is estimated well and has good performance.

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Spitzer and Herschel observations of protostellar outflows in L1251B

  • Choi, Yunhee;Lee, Jeong-Eun;Green, Joel D.;Maret, Sebastien;Yang, Yau-Lun;Bergin, Edwin A.;Blake, Geoffrey A.;Boogert, Abraham. C.A.;Di Francesco, James;Evans, Neal J. II;Pontoppidan, Klaus M.;Sargent, Annelia I.
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.81.1-81.1
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    • 2017
  • L1251B is an excellent example of a small group of pre- and protostellar objects in low-mass star-forming region. Previous interferometer data with a high angular resolution resolved the outflows associated with L1251B into a few components. To understand the physical conditions of the protostellar outflows in L1251B, we mapped this region spectroscopically with Spitzer/IRS and obtained spectral line data from Herschel/PACS. Spitzer/IRS provides the S(0)-S(7) pure rotational lines of H2 as well as fine-structure emissions produced in shocks such as S, [Si II], and [Fe II] and it is a powerful tool for studying shocked interstellar gas. In addition, [O I] lines observed with Herschel/PACS are described well by J-type shock models expected in the outflows from protostars. We will present an analysis of the L1251B protostellar outflow in the H2 pure rotational lines and fine-structure emissions.

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The Efficiency of a Spring Mass Dampers System for the Control of Vibrations and Structure-borne Noise (진동 및 고체음 제어를 위한 스프링 매스댐퍼계의 효과)

  • ;;;;Heiland, D.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1993.04a
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    • pp.147-147
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    • 1993
  • All types of dynamic excitation, periodical, pulse or transient in vertical, horizontal or all three directions can be effectively reduced by vibration isolation systems. Typical elements for vibration isolation control are spring units consisting of a group of helical compression springs. In all cases of shock, transient or random excitation energy absorbing dampers have to be added to the spring units in order to reduce system response in the frequency range near the natural frequency of the isolation system. The same isolation system of spring units and viscos-dampers has been used since 1979 for passive protection of buildings and structures has been proved to by very advantageous for vibration and structure borne noise control. Not only because of high vertical flexibility of the spring units, compared for example with typical rubber or neoprene mounts out also because of the horizontal of flexibility, which can be adapted by modifying the spring dimensions to nearly every requirement. It is just normal to use the same basic elements for passive isolation as for active isolation.

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A multi-wavelength study of N63A: A SNR within an H II region in the LMC.

  • Aliste C., Rommy L.S.E.;Koo, Bon-Chul;Lee, Yong-Hyun
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.54.3-55
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    • 2017
  • The nature and physical environments of SNRs are diverse, and for this reason, the understanding of the properties of nearby SNRs is useful in interpreting the emission from SNRs in remote galaxies where we cannot resolve them. In this regard, the LMC is a unique place to study SNRs due to its proximity, location, and composition compared with our galaxy. We carried out a multi-wavelength study of SNR N63A in the LMC, a young remnant of the SN explosion of one of the most massive (> 40 Msun) stars in a cluster. It is currently expanding within a large H II region formed by OB stars in the cluster and engulfing a molecular cloud (MC). As such, N63A is a prototypical SNR showing the impact of SN explosion on the cluster and its environment. Its morphology varies strongly across the wave bands, e.g. the size in X-ray is three times larger than in optical. However, the bright optical nebula would correspond to a MC swept up by the SNR, and consequently the interaction SNR-MC is limited to the central portion of the SNR. We aimed to study the overall structure of N63A, using near-IR imaging and spectroscopic observations to obtain the physical parameters of the atomic shocks, and also to understand how the SNR- MC interaction works and reveal the structure of the shocked cloud as well as the consequences of the impact of the SNR shock on the MC, comparing information obtained in different wavelengths.

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Characterization of nucleotide-induced changes on the quaternary structure of human 70 kDa heat shock protein Hsp70.1 by analytical ultracentrifugation

  • Borges, Julio C.;Ramos, Carlos H.I.
    • BMB Reports
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    • v.42 no.3
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    • pp.166-171
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    • 2009
  • Hsp70s assist in the process of protein folding through nucleotide-controlled cycles of substrate binding and release by alternating from an ATP-bound state in which the affinity for substrate is low to an ADP-bound state in which the affinity for substrate is high. It has been long recognized that the two-domain structure of Hsp70 is critical for these regulated interactions. Therefore, it is important to obtain information about conformational changes in the relative positions of Hsp70 domains caused by nucleotide binding. In this study, analytical ultracentrifugation and dynamic light scattering were used to evaluate the effect of ADP and ATP binding on the conformation of the human stress-induced Hsp70.1 protein. The results of these experiments showed that ATP had a larger effect on the conformation of Hsp70 than ADP. In agreement with previous biochemical experiments, our results suggest that conformational changes caused by nucleotide binding are a consequence of the movement in position of both nucleotide- and substrate-binding domains.

Design of Electronic Drum Using Computer Communication Based on Arduino (아두이노에 기반한 컴퓨터 통신을 이용한 전자드럼 설계)

  • Kim, Seungmin;Yang, Jisoo;Lee, Seungjae;Kim, Jung Tae
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.489-491
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    • 2013
  • In this paper, Electronic Drum based on Arduino and Processing language to communicate with a computer is implemented. First, we made a drum pad by using piezoelectric sensors. The drum pads prevent damage to the sensor and new mechanism was fabricated to mitigate the impact structure. Arduino connected to the pad, the sensor detects a signal when the shock sends it to Arduino. The received signal of Arduino sends a signal to the computer, and the signal received is stored in the computer to output sound of the drum. Through this structure, the micro-controller, the computer and communications technology can be combined and applicable to a many system.

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