• 대한본초학회지
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• 제31권5호
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• pp.93-98
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• 2016

Objectives : Hwangheuk-san is a complex prescription composed of oriental traditional medicine and has been reported for antioxidant, antimicrobial and anticancer effects in the recent study. Methicillin-resistant Staphylococcus aureus (MRSA) is one of important causes of fatal infectious diseases such as septicemia, endocarditis, toxic shock syndrome, pneumonia, skin and soft tissue infections (SSTIs). S. aureus is reported as being for a variety of human diseases and its epidemiological relevance is mainly due to their ability of becoming highly resistant to common antimicrobials such as tetracycline, penicillin, cphalosporin and aminoglycoside. The objective of this study is to determine the antimicrobial effect of Hwangheuk-san ethanol extracts (HHS) and synergistic effects with antibiotics oxacillin against MRSA.Methods : The antimicrobial activity of HHS was measured by the disc diffusion method, broth microdilution method and the checkerboard dilution test, time-kill curve assay was performed to investigate synergistic effects with antibiotics oxacillin against MRSA.Results : HHS showed antimicrobial activity against MRSA with a MIC value of 125 ㎍/㎖. In the checkerboard test, the interaction of HHS with antibiotics oxacillin produced almost synergy or partial synergy against MRSA. This study showed that HHS reduced the MICs of oxacillin tested, and a remarkable antibacterial effect of HHS, with membrane permeability enhancers.Conclusions : These results suggest that HHS has the antimicrobial effect and synergistic effects with antibiotics oxacillin against MRSA. This study thus can be a valuable source for the development of a new drug with low MRSA resistance.

• 한국철도학회논문집
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• 제18권3호
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• pp.175-185
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• 2015

일반적으로 열차분야에서 분기기 시스템은 안전성과 직접적으로 연관되기 때문에 높은 신뢰성이 요구된다. 특히 자기부상열차의 분기기 시스템은 대차가 궤도를 감싸는 구조적 특징으로, 고가궤도 전체가 움직여야 한다. 이러한 이유로 자기부상열차의 분기기는 강 재질로 설계되었다. 강 거더의 분기기는 콘크리트 거더에 비하여 진동측면뿐만 아니라 거더의 처짐에도 취약하다. 그러므로 자기부상열차가 유연한 분기기 위를 통과 할 때, 부상안정성 예측이 매우 중요하다. 본 논문의 목표는 자기부상열차와 분기기 거더의 연성된 동역학 해석모델을 개발하고, 공진예측 및 차량의 부상안정성 예측에 있다. 이를 위해서 차량의 3차원 다물체 동역학 모델을 개발 하였고, 분기기 거더와 모달중첩법을 이용한 연성모델링을 수행하였다. 개발된 해석모델은 실측 실험과 비교를 통해서 해석모델의 타당성을 검증하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.44-45
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• 2010

We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.

• 해양환경안전학회지
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• 제25권2호
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• pp.178-188
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• 2019

충돌 피항 동작은 선박 간 끊임없이 영향을 주고받는다. 특히 다수의 선박이 조우하는 경우, 상대 선박의 피항 의도를 파악하고 서로에게 얼마나 영향을 미치는 지를 파악하는 것은 어려운 일이다. 이를 위해 분산 확률 탐색 알고리즘이 제안되었다. 분산 확률 탐색 알고리즘은 이웃 선박과 반복적인 메시지 교환을 통해 비용을 가장 크게 낮출 수 있는 코스를 탐색 후 확률과 제한 조건에 따라 기존의 코스를 유지할지 아니면 새로운 코스를 선택할지를 결정한다. 그러나 분산 확률 탐색 알고리즘에 사용된 파라미터가 충돌 피항에 어떠한 영향을 미치는지 증명되지 않았다. 본 논문에서는 분산 확률 탐색 알고리즘의 파라미터와 가중치가 충돌 피항에 어떠한 영향을 미치는지 분석하였다. 또한 타선과의 피항 거리를 조절하기 위한 충격 흡수 영역을 소개한다. 실험 방법은 두 선박이 조우할 수 있는 세 가지 상황, 즉 정면에서 조우하는 상황, 횡단하는 상황, 추월하는 상황에 파라미터와 가중치의 변수들을 조합하여 실험을 진행하였다. 각 상황 당 8,000회, 총 24,000회의 실험이 진행되었다. 실험 결과 모든 실험에서 한 건의 충돌도 발생하지 않았다. 선박이 목적지에 큰 가중치를 줄 경우, 즉 이기적인 행동을 할 경우, 비용은 증가함을 보였다. 타선의 움직임을 더 길게 예측할수록 항행 거리, 메시지 교환 횟수는 작아지는 경향을 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권6호
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• pp.904-911
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• 2019

Objective: The study was conducted to evaluate the effects of night light regimen on growth performance, antioxidant status and health of Lingnan Yellow broiler chickens from 1 to 21 days of age. Methods: A completely randomized factorial design involved 2 photoperiods (constant lighting [CL], 24 L:0 D and intermittent lighting [INL], 17 L:3 D:1 L:3 D)${\times}2$ light intensities (10 lx and 30 lx). A total of one thousand six hundred and eighty 1-d-old Lingnan Yellow broiler chicks were randomly divided into 4 treatments with 6 replicates (70 birds per replicate). The experiment lasted for 21 d. Results: Photoperiods and light intensities had no effect on average daily gain, feed conversion ratio, and mortality of the broiler chickens (p>0.05). The INL had a significant effect on average daily feed intake (p<0.05) of broiler chickens compared with CL. Photoperiod and light intensity had an interactive effect on melatonin (MT) concentration (p<0.05). At CL, reducing light intensity increased MT concentration; INL birds had higher MT but MT concentration was not affected by light intensity. There was an interactive effect on glutathione peroxidase (GPx) and catalase (CAT) in serum and total antioxidant capability (T-AOC) in liver between photoperiod and light intensity. With the decrease of light intensity, the activities of GPx and CAT in serum and T-AOC in liver increased in CL group (p<0.05). Broiler chickens reared under INL had better antioxidant status and 10 lx treatments had higher activities of CAT in serum than 30 lx (p<0.05). Different photoperiods and light intensities had no effect on malondialdehyde. There was an interaction between photoperiod and light intensity on serum creatine kinase (CK) concentration (p<0.05). At CL, the elevated light intensity resulted in an increase in CK content; INL birds had lower CK concentration especially in low light intensity group. Besides, INL and low light intensity significantly reduced the concentration of serum corticosterone and heat shock protein 70 (p<0.05). Serum immunoglobulin M contents were increased in broiler chickens reared under the INL compared with CL group (p<0.05). Conclusion: Results above suggest that the night light regimen of INL and 10 lx could be beneficial to the broiler chickens from 1 to 21 days of age due to the better health status and electricity savings.

• 국제지역연구
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• 제21권4호
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• pp.223-245
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• 2017

본 연구의 목적은 다변량 GARCH(DCC)모형을 이용하여 탄소시장과 주식시장간 상호 영향력을 분석하는 데 있다. 탄소시장은 유럽 배출권거래제(EU ETS)의 탄소가격을, 주식시장은 에너지 소비와 탄소 배출이 상대적으로 많은 유럽의 철강 및 시멘트부문의 주식가격을 대상으로 하였다. 또한, 상관계수 분석을 통하여 시장간 상관관계 변화를 분석하였다. 이를 통해, 새로운 상품인 탄소시장의 시장성 확대(또는 배출저감 확대) 여부를 확인해 보았다. 기존 연구와 달리, (1) 배출권거래제 시작부터 2단계가 완료된 시점인 2012년까지의 자료를 모두 활용하며 (2) 전력부문에 집중된 기존연구와 달리 두 번째로 에너지 다소비 탄소 다배출부문인 철강과 시멘트부문을 대상으로 하며 (3) 수익률의 영향뿐만 아니라 변동성의 파급효과를 추가하며 (4) 시장의 동시적 상관성을 고려하여 다변량 변동성 모형인 DCC(Dynamic Conditional Correlation) 모형을 활용하였다. 분석결과, 가격 전이효과가 미미하고, 변동성 전이효과도 금융위기라는 외부충격에 의해 2단계에서 소멸하여 양 시장간에는 약한 상관관계를 유지하는 것으로 나타났다. 탄소시장과 주식시장간 상관관계는 약간 상승하는 추이를 보이지만 뚜렷한 변화를 발견하지 못하였다. 따라서, 탄소배출권은 하나의 상품으로서 금융시장으로의 시장성 확대가 미진한 것으로, 또는 배출저감이라는 본연의 역할을 수행하지 못하고 있는 것으로 해석할 수 있겠다.

• International Journal of Industrial Entomology and Biomaterials
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• 제46권1호
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• pp.16-23
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• 2023

The silkworm's dormancy and embryonic development are accomplished through the interaction of various genes. Analysis of the expression of several interacting genes can predict the embryonic stage of silkworms. In this study, we analyzed the changes in the expression level of genes at each stage during the embryonic development of dormant silkworm eggs and selected genes that can predict the hatching time. Jam123 and Jam124 silkworms were collected after egg laying, and the silkworm eggs were preserved using a double refrigeration method and expression analysis was performed for 23 genes during embryogenesis. There were 5 genes showing significant changes during embryogenesis: UDP-glucuronosyltransferases (BmUGTs), heat shock protein hsp20.8 (BmHsp20.8), Cytochromes b5-like proteins (BmCytb5), Krüppel homolog 1 (BmKr-h1), and cuticular protein RR-1 motif 41 (BmCpr41). As a result of quantitative comparison of the expression levels of these 5 genes through real-time PCR, the BmUGTs gene showed a difference between Jam123 and Jam124, making it difficult to see it as an indicator for predicting hatching time. However, the BmHsp20.8 gene had a common expression decreased at the imminent hatching stage. In addition, it was confirmed that the expression level of the BmCytb5 gene decreased to the lowest level at the time of imminent hatching, and the expression of the BmKr-h gene was made only at the time of imminent hatching. The expression of the last BmCpr41 gene can be confirmed only at the time of imminent hatching, and it was confirmed that it shows a rapid increase right before hatching. Taken together, these results suggest that expression analysis of BmHsp20.8, BmCytb5, BmKr-h1, and BmCpr41 genes can determine the stage of embryogenesis, predict hatching time, which facilitate better management of silkworm eggs.

• Journal of Astronomy and Space Sciences
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• 제22권4호
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• pp.419-430
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• 2005

이 연구에서는 지자기 폭풍의 주상 기간 동안 발생하는 태양풍 동압력 펄스에 대한 통계적 분석을 하였다. 이를 위해 먼저 1997년부터 2001년까지 5년간의 기간으로부터 지자기 폭풍 지수인 Dst 값이 -50nT 이하인 지자기 폭풍을 모두 111개 얻었다. 이러한 지자기 폭풍의 주상기간 동안에 발생한 태양풍 동압력 펄스를 정확히 조사하기 위해 태양풍 자료 뿐만 아니라 지구 저위도 여러 관측소에서 관측된 지자기 수평 성분 H값을 이용하였다. 즉 동압력 펄스가 자기권에 충돌하면 저위도 H 값이 전 지구적인 증가를 보여야 한다는 사실을 이용하였다. 이러한 과정을 통해 얻은 통계적 결과는 다음과 같다. 첫째, 자기 폭풍 중에 발생하는 H의 증가는 평균적으로 그 크기가 자기 폭풍의 강도와 비례하는 경향을 보인다. 이는 강한 자기폭풍일 수록 강한 태양풍 펄스를 동반한다는 것이다. 둘째로 자기폭풍 중에 발생하는 동압력 펄스의 발생 빈도 역시 자기 폭풍의 강도와 비례한다. 셋째, 동압력 펄스 발생 빈도가 0.4회/hr 이상인, 즉 2.5시간에 1회 이상의 동압력 펄스를 동반하는, 지자기 폭풍은 여기서 다루어진 전체 지자기 폭풍 중 약 $30\%$를 차지한다. 2.5시간은 서브스톰의 평균 지속 시간으로 볼 수 있으며, 따라서 자기 폭풍중에 서브스톰이 연속적으로 발생하는 것 만큼 자주 동압력 펄스가 나타나는 자기폭풍이 전체의 $30\%$라는 것이다. 한편 이러한 동압력 펄스의 기원을 이해하기 위해 먼저 지자기 폭풍 유도체에 대해 조사하였다. 그 결과 여기서 다루어진 지자기 폭풍의 약 $65\%$가 CME(Coronal Mass Ejection)에 의해 발생되었고 CIR(Corotating Interaction Regions)과 Type II bursts에 의해 발생한 것이 각각 6.3, $7.2\%$인 것으로 나타났다. 그런데 CME에 의해 발생된 지자기폭풍 중에서 $70\%$ 이상이 그 주상 기간이 CME와 충격파 사이의 공간인 sheath 영역 혹은 CME 앞부분에 해당되는 것으로 나타났다. 따라서 이들 지자기폭풍 주상기간에 빈번히 발생하는 동압력 펄스는 CME와 충격파 사이의 sheath 영역, 그리고 CME 앞부분 영역에서의 빈번한 태양풍 밀도 증가에 기인하는 것으로 보인다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2003년도 제20회 춘계학술대회 논문집
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• pp.91-93
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• 2003

A comprehensive numerical study is carried out to investigate for the understanding of the flow evolution and flame development in a supersonic combustor with normal injection of ncumally injecting hydrogen in airsupersonic flows. The formulation treats the complete conservation equations of mass, momentum, energy, and species concentration for a multi-component chemically reacting system. For the numerical simulation of supersonic combustion, multi-species Navier-Stokes equations and detailed chemistry of H2-Air is considered. It also accommodates a finite-rate chemical kinetics mechanism of hydrogen-air combustion GRI-Mech. 2.11[1], which consists of nine species and twenty-five reaction steps. Turbulence closure is achieved by means of a k-two-equation model (2). The governing equations are spatially discretized using a finite-volume approach, and temporally integrated by means of a second-order accurate implicit scheme (3-5).The supersonic combustor consists of a flat channel of 10 cm height and a fuel-injection slit of 0.1 cm width located at 10 cm downstream of the inlet. A cavity of 5 cm height and 20 cm width is installed at 15 cm downstream of the injection slit. A total of 936160 grids are used for the main-combustor flow passage, and 159161 grids for the cavity. The grids are clustered in the flow direction near the fuel injector and cavity, as well as in the vertical direction near the bottom wall. The no-slip and adiabatic conditions are assumed throughout the entire wall boundary. As a specific example, the inflow Mach number is assumed to be 3, and the temperature and pressure are 600 K and 0.1 MPa, respectively. Gaseous hydrogen at a temperature of 151.5 K is injected normal to the wall from a choked injector.A series of calculations were carried out by varying the fuel injection pressure from 0.5 to 1.5MPa. This amounts to changing the fuel mass flow rate or the overall equivalence ratio for different operating regimes. Figure 1 shows the instantaneous temperature fields in the supersonic combustor at four different conditions. The dark blue region represents the hot burned gases. At the fuel injection pressure of 0.5 MPa, the flame is stably anchored, but the flow field exhibits a high-amplitude oscillation. At the fuel injection pressure of 1.0 MPa, the Mach reflection occurs ahead of the injector. The interaction between the incoming air and the injection flow becomes much more complex, and the fuel/air mixing is strongly enhanced. The Mach reflection oscillates and results in a strong fluctuation in the combustor wall pressure. At the fuel injection pressure of 1.5MPa, the flow inside the combustor becomes nearly choked and the Mach reflection is displaced forward. The leading shock wave moves slowly toward the inlet, and eventually causes the combustor-upstart due to the thermal choking. The cavity appears to play a secondary role in driving the flow unsteadiness, in spite of its influence on the fuel/air mixing and flame evolution. Further investigation is necessary on this issue. The present study features detailed resolution of the flow and flame dynamics in the combustor, which was not typically available in most of the previous works. In particular, the oscillatory flow characteristics are captured at a scale sufficient to identify the underlying physical mechanisms. Much of the flow unsteadiness is not related to the cavity, but rather to the intrinsic unsteadiness in the flowfield, as also shown experimentally by Ben-Yakar et al. [6], The interactions between the unsteady flow and flame evolution may cause a large excursion of flow oscillation. The work appears to be the first of its kind in the numerical study of combustion oscillations in a supersonic combustor, although a similar phenomenon was previously reported experimentally. A more comprehensive discussion will be given in the final paper presented at the colloquium.