• Title/Summary/Keyword: Shiva

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Introduction of Shiva Gene into tobacco and Potato Using Tissue-Specific Tomato PAL Promoter (조직특이성 promoter를 이용한 Shiva 유전자의 식물체내 도입)

  • 이정윤;이신우;박권우
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.2
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    • pp.109-113
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    • 1998
  • In this study we tried to transform an antimicrobial peptide gene (Shiva) under the promoter of tomato phenylalanine ammonia-lyase (tPAL5) into tobacco and potato plants. Antimicrobial peptide gene was isolated originally from giant silk moth (Hyalophora cecropia) and modified ie nucleotide sequence to increase antimicrobial activity. Transgenic tobacco plants were regenerated and their seeds were tested on the media containing kanamycin (500 mg/L). The results of PCR amplification and genomic Southern blot hybridization confirmed the integration of construct (tPAL5 promoter-Shiva-NOS-GUS-NOS) into chromosome. We observed that one of the transgenic tobacco plants showed chromosome rearrangement when integrated. In case of potato transformation, the efficiency of regeneration was maximized at the medium containing Zeatin 2mg/L, NAA 0.01mg/L, GA$_3$ 0.1mg/L. We also observed the high expression of GUS (${\beta}$-glucuronidase) enzyme which was located next to the terminator sequence of nopaline synthase gene (NOS) in the vascular tissue of stem, leaves of transgenic potatoes. This result suggested that a short sequence of Shiva gene (120 bp) and NOS terminator sequence might be served as a leader sequence of transcript when translated.

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Zinc (II) [tetra(4-methylphenyl)] Porphyrin: a Novel and Reusable Catalyst for Efficient Synthesis of 2,4,5-trisubstituted Imidazoles Under Ultrasound Irradiation

  • Safari, Javad;Khalili, Shiva Dehghan;Banitaba, Sayed Hossein;Dehghani, Hossein
    • Journal of the Korean Chemical Society
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    • v.55 no.5
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    • pp.787-793
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    • 2011
  • An efficient three-component one-step synthesis of 2,4,5-trisubstituted imidazoles by condensation reaction of 1,2-diketones or ${\alpha}$-hydroxyketones with aromatic aldehydes and ammonium acetate using Zinc (II) [tetra (4-methylphenyl)] porphyrin as a novel and reusable catalyst under ultrasound irradiation at ambient temperature is described. In this method, ${\alpha}$-hydroxyketones as well as 1,2-diketones were converted to their corresponding 2,4,5-trisubstituted imidazoles in excellent yields.

Nanofiller as Crosslinker for Halogen-Containing Elastomers

  • Sahoo, N.G.;Kumar, E.Shiva;Das, C.K.;Panda, A.B.;Pramanik, P.
    • Macromolecular Research
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    • v.11 no.6
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    • pp.506-510
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    • 2003
  • A Zn ion-coated nanosilica filler has been developed and tested, in chlorosulfonated polyethylene (CSPE) and polychloroprene (CR), as a vulcanizing activator, cum was reinforcing filler. In this study, ZnO was replaced by the Zn ion-coated nanosilica filler with an aim of studying the dual role of this nanofiller in CSPE and CR. In the case of CSPE vulcanizates, the presence of MgO deteriorated the state and rate of cure when the Zn ion-coated nanosilica filler was used, but in the case of CR it improved the state of cure and enhanced the modulus and tensile strength. The Zn ion-coated filler proved to be a better reinforcing-cum-curing agent than was externally added ZnO and NA-22 also proved to be a better curative in the presence of the Zn ion-coated nanosilica filler for both CSPE and CR.

A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus

  • Khodabakhsh, Tannaz;Arabi, Azin;Pakzad, Parviz;Gheflat, ShivaSadat;Bahreinipour, Akram;Bandehpour, Mojgan
    • Microbiology and Biotechnology Letters
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    • v.47 no.1
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    • pp.158-163
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    • 2019
  • Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.

CURVATURES OF SEMI-SYMMETRIC METRIC CONNECTIONS ON STATISTICAL MANIFOLDS

  • Balgeshir, Mohammad Bagher Kazemi;Salahvarzi, Shiva
    • Communications of the Korean Mathematical Society
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    • v.36 no.1
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    • pp.149-164
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    • 2021
  • By using a statistical connection, we define a semi-symmetric metric connection on statistical manifolds and study the geometry of these manifolds and their submanifolds. We show the symmetry properties of the curvature tensor with respect to the semi-symmetric metric connections. Also, we prove the induced connection on a submanifold with respect to a semi-symmetric metric connection is a semi-symmetric metric connection and the second fundamental form coincides with the second fundamental form of the Levi-Civita connection. Furthermore, we obtain the Gauss, Codazzi and Ricci equations with respect to the new connection. Finally, we construct non-trivial examples of statistical manifolds admitting a semi-symmetric metric connection.

DIFFUSIVE AND STOCHASTIC ANALYSIS OF LOKTA-VOLTERRA MODEL WITH BIFURCATION

  • C.V. PAVAN KUMAR;G. RANJITH KUMAR;KALYAN DAS;K. SHIVA REDDY;MD. HAIDER ALI BISWAS
    • Journal of applied mathematics & informatics
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    • v.41 no.1
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    • pp.11-31
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    • 2023
  • The paper presents a critical analysis of selected topics related to the modeling of interacting species in which prey has nonlinear reproduction, which is in competition with predator. The mathematical model's stochastic stability is investigated. The method of designing appropriate Lyapunov functions is used to identify permanence conditions among the parameters of the model and conditions for the structure to no longer be extinct. The system's two-dimensional diffusive stability is regarded and studied. The system experiences the process of saddle-node bifurcation by varying the death rate of predator parameter. Further effects of parameters that undergo inherent oscillations are numerically investigated, revealing that as the intensity of predation parameter b is increased, the device encounters non-periodic and damped oscillations.