• 제목/요약/키워드: Shin-Hur

검색결과 581건 처리시간 0.034초

Effects of variation in the number and developmental stage of donor embryos and ovulation status of the surrogate mother on the efficiency of pig somatic cell cloning

  • Park, Mi-Ryung;Yoo, Jae Gyu;Hur, Chang-Gi;Sim, Bo-Woong;Kim, Myunghoo;Seo, Jakyeom;Kim, Byeong-Woo;Cho, Byung-Wook;Shin, Teak-Soon;Cho, Seong-Keun
    • 한국동물생명공학회지
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    • 제35권3호
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    • pp.258-264
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    • 2020
  • This study investigated the effect of variation in the number of somatic-cell-cloned embryos and their developmental stage at transfer on pregnancy, as well as the influence of the estrus status of recipient pigs on in vivo development of cloned porcine embryos after embryo transfer. For somatic cell nuclear transfer (SCNT), fibroblast cells were obtained from a male porcine fetus. Recipient oocytes were collected from prepubertal gilts at a local abattoir and then cultured. After SCNT, reconstructed embryos of different numbers and developmental stages were transferred into recipient pigs. The developmental stage of the cloned embryos and the number of transferred embryos per surrogate showed no significant differences in terms of the resulting cloning efficiency. However, the pregnancy rate improved gradually as the number of transferred cloned embryos was increased from 100-150 or 151-200 to 201-300 per recipient. In pre-, peri-, and post-ovulation stages, pregnancy rates of 28.6%, 41.8%, and 67.6% and 16, 52, and 74 offspring were recorded, respectively. The number of cloned embryos and estrus status of the recipient pig at the time of transfer of the cloned embryo affect the efficiency of pig production; therefore, these variables should be particularly considered in order to increase the efficiency of somatic cell pig cloning.

Long-term Activation of c-Jun N-terminal Kinase through Receptor Interacting Protein is Associated with DNA Damage-induced Cell Death

  • Seok, Jeong-Ho;Park, Kyeong-Ah;Byun, Hee-Sun;Won, Min-Ho;Shin, Sang-Hee;Choi, Byung-Lyul;Lee, Hyun-Ji;Kim, Young-Rae;Hong, Jang-Hee;Park, Jong-Sun;Hur, Gang-Min
    • The Korean Journal of Physiology and Pharmacology
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    • 제12권4호
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    • pp.185-191
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    • 2008
  • Activation of c-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase family, is an important cellular response that modulates the outcome of the cells which are exposed to the tumor necrosis factor (TNF) or the genotoxic stress including DNA damaging agents. Although it is known that JNK is activated in response to genotoxic stress, neither the pathways to transduce signals to activate JNK nor the primary sensors of the cells that trigger the stress response have been identified. Here, we report that the receptor interacting protein (RIP), a key adaptor protein of TNF signaling, was required to activate JNK in the cells treated with certain DNA damaging agents such as adriamycin (Adr) and 1-${\beta}$-D-arabinofuranosylcytosine (Ara-C) that cause slow and sustained activation, but it was not required when treated with N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and short wavelength UV, which causes quick and transient activation. Our findings revealed that this sustained JNK activation was not mediated by the TNF (tumor necrosis factor) receptor signaling, but it required a functional ATM (ataxia telangiectasia) activity. In addition, JNK inhibitor SP-600125 significantly blocked the Adr-induced cell death, but it did not affect the cell death induced by MNNG. These findings suggest that the sustained activation of JNK mediated by RIP plays an important role in the DNA damage-induced cell death, and that the duration of JNK activation relays a different stress response to determine the cell fate.

요추부 수술 후 경막외 무통방법과 지속적 정맥 주입에 의한 통증 조절의 효과 비교 (Effects of Continuous Intravenous Analgesia Versus Epidural Analgesia after Lumbar Spinal Surgery : A Prospective Study)

  • 오규성;신문수;길현주;허륭;최훈규;안정용;권성오
    • Journal of Korean Neurosurgical Society
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    • 제30권12호
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    • pp.1394-1398
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    • 2001
  • Objective : The purpose of this non-randomized prospective study was to evaluate the safety and efficacy of continuous intravenous nalbuphine-ketorolac-droperidol(CIA) versus continuous infusion of epidural morphine-bupivacaine(CEA) for pain control after lumbar spinal surgery. Methods : Twenty-one patients who underwent spine surgery including laminectomy, fusion with fixation were assigned to receive an intravenous bolus of nalbuphine 5mg and ketorolac 15mg, followed by a continuous infusion of nalbuphine 25mg, ketorolac 105mg, and droperidol 5mg mixed with normal saline 98cc(2cc/hr). Twenty patients received a bolus infusion of morphine 2mg and 0.125% bupivacaine 8cc followed by a continuous intravenous infusion of 100cc 0.125% bupivacaine and morphine sulfate 8.0mg(2cc/hr). Pain score was measured on a visual analogue scale(VAS). It's safety and efficacies were compared with the results of continuous infusion of epidural morphine-bupivacaine, which was reported previously by same authors. A continuous infuser was used to give epidural morphine-bupivacaine and intravenous nalbuphine-ketorolac-droperidol. Results : In general, mild pain, pain less than 3 VAS scores, was observed postoperatively from 30minutes to 72hours in CEA group, and from 6 hours to 72 hours in CIA group. The early postoperative pain was controlled easily in 6 hours in CEA group, compared to CIA group(p<0.05). However, there was no statistical significance in 72 hours on pain scores between CEA and CIA groups after 6-12hours of pain managements. Pruritus, nausea and vomiting, and urinary retention were more frequent in CEA group. Conclusion : CIA and CEA are considered effective methods in postoperative pain managements. However, adequate doses in early intravenous infusion and continuous intravenous analgesia with nalbuphine-ketorolac-droperidol will be needed for better control in early postoperative pain with less side effects.

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Agricultural Characters, Phenolic and Nutritional Contents, and Antioxidant Activities of Pigeon Pea (Cajanus cajan) Germplasms Cultivated in the Republic of Korea

  • Choi, Yu-Mi;Hyun, Do Yoon;Lee, Sukyeung;Yoon, Hyemyeong;Lee, Myung-Chul;Oh, Sejong;Ko, Ho-Cheol;Shin, Myoung-Jae;Hur, Onsook;Yi, Jung Yoon;Desta, Kebede Taye
    • 한국자원식물학회지
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    • 제33권1호
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    • pp.50-61
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    • 2020
  • Pigeon pea (Cajanus cajan) is an important legume species that produces seeds that are rich in phenolic compounds and dietary nutrients. The aim of the present study was to evaluate the agricultural characters, phenolic and nutritional contents, and antioxidant capacities of seeds from 82 pigeon pea germplasms cultivated in the Republic of Korea. The accessions exhibited compact (24.39%), semi-spreading (74.39%), or spreading (1.22%) growth habits and determinate (89.02%), indeterminate (8.54%), or semi-determinate (2.44%) flowering patterns. Days to 75% maturity ranged from 30 to 72 d, and yield per plant ranged from 6.00 to 148.60 g. Meanwhile, total phenolic, crude protein, crude fiber, and dietary fiber contents ranged from 16.42 ± 0.62 to 29.67 ± 0.43 mg gallic acid equivalent per g of dried extract, from 16.76 ± 6.74% to 22.61 ± 0.05%, from 4.70 ± 0.24 to 8.63 ± 0.02%, and from 12.98 ± 0.71 to 33.19 ± 1.50%, respectively. In addition, DPPH radical-scavenging capacity ranged from 1.61 ± 0.10 to 16.04 ± 2.30 mg ascorbic acid equivalent per g of dried extract, and Trolox equivalent antioxidant capacity ranged from 3.03 ± 0.86 to 42.24 ± 0.72 mg Trolox equivalent per g of dried extract. Phenolic content was correlated with both DPPH radical-scavenging capacity (r = 0.63) and Trolox equivalent antioxidant capacity (r = 0.29). Nine accessions (IT170290, IT170291, IT170270, IT170276, IT170379, IT170386, IT170388, IT170418, and IT170340) exhibited early maturity, compact and erect growth habits, and above average antioxidant activities and phenolic and protein contents. In addition, accessions IT170290 and IT170291 were especially promising pigeon pea germplasms to grow, owing to various favorable characteristics (e.g., high yield and dietary fiber content). Hence, these accessions could be useful cultivars to the Republic of Korea if considered in future agricultural systems.

유라시아 교통물류 네트워크 기반 구축을 위한 한국적 시각에서의 정보공유 플랫폼 도입방안 (An Information Sharing Platform for Eurasia Transportation Logistics Network from Korean perspective)

  • 신승진;노홍승;허성호
    • 한국ITS학회 논문지
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    • 제17권5호
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    • pp.100-116
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    • 2018
  • 최근 미국의 보호무역 기조에 따라 대미수출이 위축되어 유라시아 신흥시장의 개척 필요성이 한층 커졌고, 대형 국적선사인 한진해운의 파산으로 인해 아시아-유럽 해운항로를 대체할 수 있는 운송로 확보의 필요성이 대두되었다. 더욱이 전자상거래의 확산으로 인해 국제교역 및 물류환경이 급변하고 있어 국제 물류활동에 IT기술을 적용하는 환경에 대비해야 하는 시점이다. 따라서 본 연구는 유라시아 각 국의 물류정보 수준비교를 통해 한국의 시각에서 유라시아 물류정보 공유플랫폼 정보화전략을 수립하는 차원에서 수행되었다. 본 연구는 유라시아 국가 간 물류활동 지원을 위한 표준화 방향, 단절 없는 통관 물류서비스 제공, 무역업체의 비즈니스 확대를 위한 정보제공, 국가 간 물류기업 간 협력확대를 위한 정보연계 기반구축 등을 목표로 한다. 본 연구를 통해 국가 간 정보공유를 통한 물류활동이 활발히 이루어지기를 기대한다.

온대 활엽수림에 서식하는 산림성 조류의 가장자리 선호도 분석 (Edge Preference of Forest-dwelling Birds in Temperate Deciduous Forests)

  • 최창용;남현영;허위행;이우신;김현중;황근연
    • Journal of Ecology and Environment
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    • 제29권3호
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    • pp.191-203
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    • 2006
  • 본 연구는 중부 온대활엽수림을 대표하는 광릉 숲을 대상으로 산림성 조류가 숲의 내부와 가장자리를 어떻게 이용하는지 분석하고, 숲의 단편화로 인한 영향을 파악할 수 있도록 가장 자리 선호도에 따라 서식지 특이종을 파악하기 위해 실시되었다. 그 결과 조류의 풍부도는 숲의 내부와 가장자리에서 일관성 있는 결과를 얻지 못하였으며, 이는 숲 내부와 가장자리를 모두 이용할 수 있는 일반종의 출현 여부에 의해 크게 좌우되었다. 따라서 산림의 단편화가 산림성 조류에 미치는 영향을 파악하기 위해서는 전체 조류군집의 풍부도를 확인하는 것보다 특정 환경을 선호하는 서식지 특이종의 변화를 집중적으로 파악하는 것이 유리할 것으로 판단되었다. 선 조사법과 정점 조사법에 의해 얻어진 숲 내부와 가장자리의 서식밀도를 기준으로 서식지 특이종을 파악한 결과 숲새(U. squameiceps), 흰배 지빠귀(T. pallidus), 진박새(P. ater) 등 3 종이 숲 내부종으로 나타났으며 까치(P. pica), 꾀꼬리(O. chinensis)의 2 종은 숲 가장자리종으로 나타났다. 따라서 광릉 숲으로 대표되는 중부 온대활엽수림의 단편화에 의한 산림성 조류의 영향을 파악하기 위해서는 서식지 특이종으로 파악된 종을 대상으로 한 분포와 번식 등에 대한 모니터링이 필요하며, 특히 숲의 내부에 강한 선호도를 보이는 종에 대한 조사가 우선되어야 할 것으로 판단된다.

냉동치료로 치료된 비전형적 기관지 유암종 1예 (A Case of Atypical Bronchial Carcinoid Treated by Cryotherapy)

  • 김혜옥;김세중;이승현;이은주;허규영;이승룡;이상엽;김제형;신철;심재정;인광호;강경호;유세화
    • Tuberculosis and Respiratory Diseases
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    • 제70권6호
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    • pp.521-525
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    • 2011
  • Bronchial carcinoid tumors are relatively uncommon neoplasms that are considered to be malignant tumors of low to intermediate grade. They are classified by pathologic features as typical or atypical carcinoids and have distinctly different prognoses and therapeutic options. Surgery is the treatment of choice in typical and atypical carcinoid tumors but the approach has been changing. Recently, several studies have described experiences using other technologies as adjuncts to bronchoscopic resection, technologies such as laser and cryotherapy with curative intent in endoluminal typical carcinoids. Here we present a case of atypical bronchial carcinoid that was treated with bronchoscopic cryotherapy.

우유빛 기관지 세척액 소견을 보인 양측성 폐침윤 (Bilateral Pulmonary Infiltrate with Milky BAL Fluid)

  • 윤기철;문재영;김혜옥;김정하;하은실;이경주;이은주;허규영;강은해;정기환;이승룡;김제형;이상엽;신철;심재정;인광호;강경호;유세화
    • Tuberculosis and Respiratory Diseases
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    • 제65권3호
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    • pp.239-242
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    • 2008
  • 외인성 지방성 폐렴은 자세한 병력 청취 없이는 의심하기 힘든 비교적 드문 질환이다. 저자들은 비특이적인 임상 증상과 방사선 소견을 보인 환자에서 스쿠알렌을 섭취한 과거력에 주목하였으며 외인성 지방성 폐렴을 의심 하에 기관지폐세척술 및 폐 생검을 통하여 확진하였다.

편측성 종격동 종괴와 반대측 흉수로 발현된 기형종 1예 (Teratoma Presenting as An Unilateral Mediastinal Mass with Contralateral Pleural Effusion)

  • 하은실;허규영;정기환;이승룡;조원민;이상엽;김제형;이응석;신철;심재정;인광호;강경호;유세화
    • Tuberculosis and Respiratory Diseases
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    • 제60권3호
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    • pp.347-352
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    • 2006
  • 저자들은 1개월간 지속된 왼쪽 어깨통증 및 갑자기 발생한 호흡곤란으로 내원한 36세 여자 환자에서, 편측성 종격동의 종괴와 흉막강으로 파열되어 반대측의 흉수를 동반한 기형종의 진단 및 치료 1예를 경험하여 문헌고찰과 함께 보고하는 바이다.

기계적 측정에 의한 호상요구르트의 관능특성 예측 (Prediction of Sensory Properties for the Stirred-type Fruit Yogurts by Instrumental Measurements)

  • 오세종;심재헌;허재관;신정걸;김상교;백영진
    • 한국식품과학회지
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    • 제25권6호
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    • pp.620-625
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    • 1993
  • 본 연구는 요구르트의 관능 특성과 기계적 측정과의 관계를 구명하여 기계적 측정으로 관능특성을 예측하기 위한 피시계수를 추정할 목적으로 실시하였다. 딸기, 복숭아, 열대 혼합과일 요구르트에 대한 관능특성은 점성, 조직감, 맛, 종합적 품질의 4항목으로하여 각각 평가하였으며, 기계적 parameter들은 Refractive index, Viscosity, Consistency, 경도, 부착성, 응집성, 탄력성, 껌성으로 특성을 산출하였다. 관능적 점성은 복숭아 요구르트가 발기와 열대 혼합과일 요구르트보다 다소 높았으며(p<.05), Refractive index와 Consistency의 경우에도 복숭아 요구르트가 가장높은 수치를 나타내었다(p<.05). 또한 경도, 부착성, 껌성에서 요구르트간 차이가 나타났다(p<.05). 관능적 점성과 기계적 측정과의 상관관제는 모든 요구르트에서 Viscosity, Consistency, 경도, 부착성과 유의적 상관관계가 있었으며(p<.05), 관능적 점성과 명성과의 상관관계는 복숭아와 열대 혼합과일 요구르트에서 나타났다(p<.05). 조직감, 맛, 품질과 기계적 측정과의 상간관계는 열대 혼합과일 요구르트에서 맛과 부착성에서만 유의적 관계(p<.05)가 나타났을 뿐 비교적 남은 관계를 보였다. 관능특성에 대한 기계적 요소의 다중회귀모형을 구한 결과, 관능적 점성$(Y_{1})$$Consistency(X_{1}),\;Viscosity(X_{2})$, 응집성($X_{3}$)으로, 조직감은 Consistency로 각각 표현될 수 있었으며, 추정된 회귀방정식은 다음과 같다.

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