• Advances in Traditional Medicine
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• v.7 no.4
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• pp.385-389
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• 2007

We undertook the present study to evaluate different pharmacological as antioxidant, antibacterial and cytotoxic activities of the crude ethanolic extract of Momordica charantia (Family: Cucurbitaceae) leaves. The antioxidant property of the extract was assessed by 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay. The extract showed antioxidant activity where $IC_{50}$ was about ${\sim}500{\mu}g/ml\;and\;IC_{50}$ was about ${\sim}10{\mu}g/ml$ for standard drug ascorbic acid. The extract showed a broad spectrum of antibacterial activity against all the tested gram positive and gram-negative bacteria where Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella dysenteriae were prominent. And the zones of inhibition were ranging from 8-15 mm for all the tested bacteria. Its cytotoxic property was evaluated by brine shrimp lethality bioassay. The extract showed significant lethality and the $LC_{50}$ value was $20{\mu}g/ml$.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.4
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• pp.575-580
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• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• Korean Journal of Microbiology
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• v.31 no.3
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• pp.245-254
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• 1993

Distribution and variation of bacterial densities of heterotrophic plate count (HPC) and Enterobacteriaceae in the water supply systems comprising raw, treated, and three tap water samples of a water treatment plant in Seoul were studied 23 times from 1991 to 1992. HPC bacteria of raw. treated, and tap waters on $R_{2}A$ agar media were at a density of $1.22{\times}10^{3} to 3.05{\times}10^{5}$, $1.50{\times}10^{1} to 4.29{\times}10^{3}$ and 2 to $5.41{\times}10^{3}$ cfu/ml, respectively. Densities of Enterobacteriaceae in raw, treated, and tap waters on mENDO-LES agar media ranged from 0.] to 8200 cfu/ml, 0 to 17.5 cfu/JOO mI. and 0 to 47.5 cfu/IOO ml, respectively. Injured Enterobacteriaceae of treated and tap waters on m-T7 agar media were at a density of o to 27 and 0 to 35 cfu/100 mI. These results showed that the density of bacteria in the treated water outflowing from the water plant significantly increased as the water flowed along the distribution sytems, which is so-called bacterial regrowth. The predominant bacteria] types in the water supply system were Pw'udomonas and Acinerobacter. In raw water, the ratio of Pseudomonas was higher than that of Acinetobaeter, but in treated and tap waters. both ratios were reversed. The most predominant species of Enterobacteriaceae was Enterobacter agglomerans. Some species such as Citrobacter freundii. Escherichia coli. Klebsiella pneumoniae. and Shigella dysenteriae which are opportunistic pathogens or pathogens were not found in the treated water but additionally detected in tap waters.

• Clinical and Experimental Pediatrics
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• v.53 no.2
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• pp.253-257
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• 2010

Hemolytic uremic syndrome (HUS) is the most common cause of acute renal failure in children younger than 4 years and is characterized by microangiopathic hemolytic anemia, acute renal failure, and thrombocytopenia. HUS associated with diarrheal prodrome is usually caused by Shiga toxin-producing Escherichia coli O157:H7 or by Shigella dysenteriae, which generally has a better outcome. However, atypical cases show a tendency to relapse with a poorer prognosis. HUS has been reported to be associated with acute lymphoblastic leukemia (ALL) in children. The characteristics and the mechanisms underlying this condition are largely unknown. In this study, we describe the case of an 11-year-old boy in whom the diagnosis of ALL was preceded by the diagnosis of atypical HUS. Thus, patients with atypical HUS should be diagnosed for the possibility of developing ALL.

• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.495-501
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• 1999

Lactobacillus acidophilus KY 2104 was isolated from 41 healthy korean ($2{\sim}35\;age$, 26 man, 15 woman) feces to use as a probiotic lactic acid bacteria for functional foods, fermented milk and pharmaceutical products. L. acidophilus KY 2104 survived in the presence of acid and bile, and inhibited pathogens. It survived almost 100% in pH 3.0 phosphate buffer for 2 hr, and more than $10^4\;cfu/mL$ in pH 2.0 phosphate buffer when inoculated by $10^7\;cfu/mL$. It was also found to grow in MRS medium containing 0.5% oxgall. L. acidophilus KY 2104 exerted inhibitory effect against enteric pathogens such as Escherichia coli, Shigella dysenteriae, Staphylococcus aureus and Salmonella typhimurium, when grown with each in associative cultures for 16 hr. As a result of acute toxicity of L. acidophilus KY 2104 in rats, there was no observation on motality, clinical signs, body weights and gross findings in the oral administration.

• The Korean Journal of Mycology
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• v.10 no.1
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• pp.27-31
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• 1982

The antibacterial activity of Poria cocos against the human pathogenic bacteria and general bacteria was observed. The results obtained were as follows; 1. There wasn't any anti­bacterial activity at the Poria cocos powders, pieces and its extracts in every different kinds and places of origin. 2. Hyphae of Poria cocos in every different kinds and places of origin had the antibacterial activity against four Gram positive bacteria, i.e., Staphylococcus aureus, S. epidermidis, Bacillus subtilis and Streptococcus faecalis, on the other hand, hadn't the antibacterial activity against six Gram negative bacteria, Salmonella typhi, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaria and Klebsiella pneumoniae. 3. The average inhibitory zone was 13.6mm for S. aureus, 14.7mm for S. epidermidis, 12.7mm for B. subtilis, and 13.2mm for S. faecalis at the concentration of $335{\mu}g$ of hyphae of Poria cocos.

• Korean Journal of Pharmacognosy
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• v.30 no.3
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• pp.340-344
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• 1999

To evaluate antimicrobial activity of Moutan cortex the compounds isolated from $CHCl_3$ and EtOAc fractions of Moutan cortex were subjected to eight pathogenic strains. Benzoic acid, witch was identified from the $CHCl_3$ fraction, had MICs with $625{\sim}1,250\;{\mu}g/ml$ against all of the strains tested. Methyl gallate, p-hydroxy benzoic acid, gallic acid and $1,2,3,4,6-penta-O-gallyol-{\beta}-D-glucose$, which were identified from the EtOAc fraction, showed the antimicrobial activity, and the methyl gallate had the widest antimicrobial activity with MICs of $625{\sim}5,000\;{\mu}g/ml$ against all strains tested. p-Hydroxy benzoic acid showed MICs of $1,250{\sim}2,500\;{\mu}g/ml$ against all of the strains tested except C.albicans. Gallic acid had the best antimicrobial activities with MICs against the Shigella dysenteriae and Streptococcus mutans-strains of 78.1 and $312.5\;{\mu}g/ml$, respectively, but not against the C. albicans. And $1,2,3,4,6-penta-O-gallyol-{\beta}-D-glucose$ had the best antimicrobial activitie with MICs against the B. cereus, Staph. epidermidis and C. albicans strains of 39.1, 39.1 and $156.3\;{\mu}g/ml$, respectively, but not against the E. coli and Shig. Dysenteriae.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.107-112
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• 2005

This study was performed to investigate the antimicrobial effect of the Hedyotis diffusa extracts against food-borne pathogens. First, the Hedyotis diffusa was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of the Hedyotis diffusa extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Hedyotis diffusa showed the highest antimicrobial activity against Staphylococcus aureus and Shigella flexneri. Synergistic effect in inhibition was observed when Hedyotis diffusa extract was mixed Sophora subprostrata extract as compared to each extracts alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of Hedyotis diffusa against Staphylococcus aureus and Salmonella Typhimurium. The methanol extract of Hedyotis diffusa had strong antimicrobial activity against Staphylococcus aureus at the concentration of 4,000 ppm. At this concentration, the growth of Staphylococcus aureus was retarded more than 36 hours and up to 24 hours for Salmonella Typhimurium. In conclusion, the methanol extracts of Hedyotis diffusa inhibit efficiently Staphylococcus aureus and Salmonella Typhimurium.

• The Korean Journal of Food And Nutrition
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• v.25 no.2
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• pp.233-238
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• 2012

Diarrheal diseases constitute one of the major causes of morbidity and mortality in infants and young children globally. One of the main microorganisms causing diarrheal diseases is Campylobacter jejuni. For treatment of these diseases, Portulaca oleracea has been widely used as a folk remedy for a long time. This study was performed to investigate the antimicrobial activity of P. oleracea against gastroenteritis pathogens including C. jejuni. P. oleracea was extracted with petroleum ether, chloroform, ethylacetate, methanol, and hot water. The antimicrobial activity of the P. oleracea extracts was determined using the paper disc method, minimum inhibitory concentration, and the liquid culture method. The 10 $mg/m{\ell}$ ethylacetate extract showed the strongest antimicrobial activity against Salmonella typhimurium, Salmonella enteriditis, and Shigella spp.. The hot water extract from P. oleracea showed the highest anti-microbial activity against C. jejuni at 10~20 $mg/m{\ell}$. The hot water extract of P. oleracea retarded the growth of C. jejuni for 36 hr at $42^{\circ}C$.

• Journal of Life Science
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• v.11 no.5
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• pp.483-488
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• 2001

The antimicrobial substance produced by Pseudomonas aeruginosa KLP-2 strain was purified and identified. The substance was identified as a pyocyanine by the fast atom bombardment mass(FAB-MS). In physic-chemical properties, the pyocyanine was dark blue needles, and was soluble in various organic solvents such as chlorogorm, methanol, ethanol and ethyl acetae. The pyocyanine possessed a ultraviolet absorbance spectrum in methanol, 0.1 M HCl, and chlorogorm. The maximum absorption peak of the pyocyanine showed at 318 mm in methanol. The molecular formula of the pyocyanine was determined to the $C_{13}$ H$_{10}$ N$_{2}$O and protonate molecular ion species (M+H)$^{+}$ was observed at m/z 211 by FAB-MS. The pyocyanine showed antimicrobial against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Rodococcus equi, Staphylococcus aureus, Streptococcus faecalis, E. col, Legionella pneumophila, Shigella flexneri Shigella boydii, shgella sonnei, NAG Vibrio cholerae, Vibrio parahaemolyticus, Vibro vulnificus, Yersinia enterocolitica, and Saccharomyces cerevisiae. However, Salmonella spp. Shigela dysenteriae, 3 strains of Pseudomonas aeruginosa, Klebsiela pneumoniae, and Aspergillus niger were resistant to the pyocyanine. The pyocyanine showed the highest antimicrobial activity aganist Legionella pneumophila based on the size of inhibition zone by the disk contained 0.5 $\mu\textrm{g}$ of the pyocyanine.e.