• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5725-5730
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• 2013

Cancer patients often suffer from local tumor recurrence after radiation therapy. Cell cycling, an intricate sequence of events which guarantees high genomic fidelity, has been suggested to affect DNA damage responses and eventual radioresistant characteristics of cancer cells. Here, we established a radioresistant lung cancer cell line, A549R, by exposing the parental A549 cells to repeated ${\gamma}$-ray irradiation with a total dose of 60 Gy. The radiosensitivity of A549 and A549R was confirmed using colony formation assays. We then focused on examination of the cell cycle distribution between A549 and A549R and found that the proportion of cells in the radioresistant S phase increased, whereas that in the radiosensitive G1 phase decreased. When A549 and A549R cells were exposed to 4 Gy irradiation the total differences in cell cycle redistribution suggested that G2-M cell cycle arrest plays a predominant role in mediating radioresistance. In order to further explore the possible mechanisms behind the cell cycle related radioresistance, we examined the expression of Cdc25 proteins which orchestrate cell cycle transitions. The results showed that expression of Cdc25c increased accompanied by the decrease of Cdc25a and we proposed that the quantity of Cdc25c, rather than activated Cdc25c or Cdc25a, determines the radioresistance of cells.

• Smart Structures and Systems
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• 제21권5호
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• pp.571-582
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• 2018

Track irregularities of high-speed Maglev lines have significant influence on ride comfort. Their adjustment is of key importance in the daily maintenance of these lines. In this study, an adjustment method is proposed and track irregularities analysis is performed. This study considers two modules: an inspection module and a vehicle-guideway coupling vibration analysis module. In the inspection module, an inertial reference method is employed for field-measurements of the Shanghai high-speed Maglev demonstration line. Then, a partial filtering, integration method, resampling method, and designed elliptic filter are employed to analyze the detection data, which reveals the required track irregularities. In the analysis module, a vehicle-guideway interaction model and an electromagnetic interaction model were developed. The influence of the measured line irregularities is considered for the calculations of the electromagnetic force. Numerical integration method was employed for the calculations. Based on the actual field detection results and analysis using the numerical model, a threshold analysis method is developed. Several irregularities modalities with different girder end's deviations were considered in the simulations. The inspection results indicated that long-wavelength irregularities with larger girder end's deviations were the dominant irregularities. In addition, the threshold analysis of the girder end's deviation shows that irregularities that have a deviation amplitude larger than 6 mm and certain modalities (e.g., M- and N-shape) are unfavorable. These types of irregularities should be adjusted during the daily maintenance.

• Asian Pacific Journal of Cancer Prevention
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• 제16권8호
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• pp.3509-3515
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• 2015

Background: Diallyl disulfide (DADS) may exert potent anticancer action both in vitro and in vivo. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between DADS and pyruvate kinase (PKM2). Materials and Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PKM2 was used with a cell counting kit (CCK)-8 and flow cytometry (FCM) to investigate the effects of DADS. Relationships between PKM2 and DADS associated with phosphorylation of EGFR, ERK1/2 and MEK, were assessed by western blot analysis. Results: In $KG1{\alpha}$ cells highly expressing PKM2, we found that DADS could affect proliferation, apoptosis and EGFR/ERK/PKM2 signaling pathways, abrogating EGF-induced nuclear accumulation of PKM2. Conclusions: These results suggested that DADS suppressed the proliferation of $KG1{\alpha}$ cells, providing evidence that its proapoptotic effects are mediated through the inhibition of EGFR/ERK/PKM2 signaling pathways.

• Journal of Power Electronics
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• 제18권3호
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• pp.841-852
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• 2018

This paper presents a dual-algorithm search method (GWO-GSO) combining grey wolf optimization (GWO) and golden-section optimization (GSO) to realize maximum power point tracking (MPPT) for photovoltaic (PV) systems. First, a modified grey wolf optimization (MGWO) is activated for the global search. In conventional GWO, wolf leaders possess the same impact on decision-making. In this paper, the decision weights of wolf leaders are automatically adjusted with hunting progression, which is conducive to accelerating hunting. At the later stage, the algorithm is switched to GSO for the local search, which play a critical role in avoiding unnecessary search and reducing the tracking time. Additionally, a novel restart judgment based on the quasi-slope of the power-voltage curve is introduced to enhance the reliability of MPPT systems. Simulation and experiment results demonstrate that the proposed algorithm can track the global maximum power point (MPP) swiftly and reliably with higher accuracy under various conditions.

• Parasites, Hosts and Diseases
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• 제35권1호
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• pp.19-24
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• 1997

The infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in Bayuquan region, Yingko city nearby the coast of the Bohai Sea from may to August 1992. A total of 7,327 laivae were collected from 156 fish of 19 species and 8 squids of one species. The 3rd-stage larvae of Anisokis simplex were collected from 121 fish (63..4%) of 15 species (N : 191) and from 8 squids (14.8%) of one species (N : %4), and they were total, 5,992 (81.8%). Out of remaining 1,335 larvae, 154 (2.1%) were classified as Rvnnqscnris type B from 23 fish of 4 species, 1,013 (13.8%) as avnnosccris type C from 79 fish of 13 species, 164 (2.2%) as Hvsterothylacium China type V from 20 fish of 4 species, 3 (0.04%) as Raphinoscnris from 3 fish of 2 species and one was Pseunotewonovc necipiens larva. Key words: Bohai Sea, Anisokinae, larvae , Anisckis, HvsterothWLacium, Thwnnascaris, Raphinqscaris , Pseudoterranova.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.59-64
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• 2011

Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at $21^{\circ}C$. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.454-464
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• 2019

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, $MgSO_4$ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as $89fg/{\mu}l$, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.

• Journal of Ginseng Research
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• 제46권4호
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• pp.543-549
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• 2022

Background: In physical activity or labor, the human body is in a state of high intensity stress, and all parts or physiological functions of the body respond positively to maintain or balance the need for movement. The human body has many physiological changes in the process of movement, and fatigue is the external manifestation of various complex changes inside the human body. Fatigue is also a physiological mechanism of self-protection after the body reaches a certain level of activity, which can prevent the occurrence of life-threatening excessive functional failure. The generation of fatigue is a very complex process, and its mechanism has not been concluded yet. Therefore, it is an important work to search and screen the effective components of natural plants that have anti-fatigue effect and to explore their mechanism. Methods: This was a 8-week, randomized, double-blind, placebo-controlled clinical trial. A total of 110 subjects who passed physical examination were included according to the scheme design, and randomly divided into a test group which was given KRG and a placebo control group. The calculation is carried out according to the standard of sub-high-intensity exercise test. Results: There was no adverse effect on safety index of subjects after taking red ginseng capsule. After KRG treatment, subjective strength grade is significant lower than placebo treatment. Blood lactic acid content is significantly get lower after trial in KRG group, and significant lower than placebo group. Creatine phosphokinase(CK) content is significantly get lower after trial in KRG group, and significant lower than placebo group. Conclusion: According to the criterion in the test scheme, the result shows that KRG is helpful on relieving physical fatigue.

• Geomechanics and Engineering
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• 제25권3호
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• pp.179-194
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• 2021

In this paper, to study the stability of surrounding rock during roadway excavation in different rock mass structures, the physical model test for roadway excavation process in three types of intact rock mass, layered rock mass and massive rock mass were carried out by using the self-developed two-dimensional simulation testing system of complex underground engineering. Firstly, based on the engineering background of a deep mine in eastern China, the similar materials of the most appropriate ratio in line with the similarity theory were tested, compared and determined. Then, the physical models of four different schemes with 1000 mm (height) × 1000 mm (length) × 250 mm (width) were constructed. Finally, the roadway excavation was carried out after applying boundary conditions to the physical model by the simulation testing system. The results indicate that the supporting effect of rockbolts has a great influence on the shallow surrounding rock, and the rock mass structure can affect the overall stability of the surrounding rock. Furthermore, the failure mechanism and bearing capacity of surrounding rock were further discussed from the comparison of stress evolution characteristics, distribution of stress arch, and failure modes in different schemes.

• Nuclear Engineering and Technology
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• 제55권8호
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• pp.2723-2733
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• 2023

Transuranic nuclides (such as ²³⁸Pu, ²⁵²Cf, ²⁴⁹Bk, etc.) have a wide range of application in industry, medicine, agriculture, and other fields. However, due to the complex conversion chain and remarkable fission losses in the process of transuranic nuclides production, the generation amounts are extremely low. High flux reactor with high neutron flux and flexible irradiation channels, is regarded as the promising candidate for producing transuranic nuclides. It is of great significance to increase the conversion ratio of transuranic nuclides, resulting in higher efficiency and better economy. In this paper, we perform an optimization design evaluation of producing transuranic nuclides in high flux reactor, which includes optimization design of irradiation target and influence study of reactor core loading. It is demonstrated that the production rate increases with appropriately determined target material and target structure. The target loading scheme in the irradiation channel also has a significant influence on the production of transuranic nuclides.