• 미생물학회지
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• 제39권3호
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• pp.175-180
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• 2003

Shewanella putrefaciens DK-1은 그람음성, 통성 혐기성 세균으로 $NO_{3}$, Fe(III), Mn(IV), humic acid와 같은 다양한 전자수용체를 이용한다. S. putrefaciens DK-1의 전자공여체의 이용능력은 제한적이며, lactate나 formate는 좋은 전자공여체로 이용되지만 acetate나 toluene은 이용하지 못하였다. 다양한 전자수용체간의 경쟁을 살펴보기 위해 전자수용체로 Fe(III)와 같이 $NO_{3}^{-}$, $NO_{2}^{-}$를 넣어 주었을 매 Fe(III)의 환원은 저해되었다. 또한 5. putrefaciens DK-1은 전자수용체로 토양에 광범위하게 존재하는 humic acid를 이용하였으며, 환원된 humic acid는 질산염에 의해서 다시 산화되었다. Fe(III) 환원능이 있는 환경 시료를 이용하여 탄소, 질소, 인과 같은 제한 요인이 Fe(III) 환원세균의 활성에 미치는 효과를 조사하였다. 천호지의 저질토와 대호의 농토에 각각 탄소원, 질소원, 인을 첨가해 주었을 경우 S. putrefaciens DK-1과 탄소원을 동시에 첨가해 주었을 때 가장 높은 철 환원능을 보여주었다.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.127-131
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• 1999

Anaerobically grown cells of an Fe(III)-reducing bacterium, Shewanella putrefaciens IR-l, were electrochemically active with an apparent reduction potential of about 0.15 V against a saturated calomel electrode in the cyclic voltammetry. The bacterium did not grow fermentatively on lactate, but grew in an anode compartment of a three-electrode electrochemical cell using lactate as an electron donor and the electrode as the electron acceptor. This property was shared by a large number of Fe(III)-reducing bacterial isolates. This is the first observation of a direct electrochemical reaction by an intact bacterial cell, which is believed to be possible due to the electron carrier(s) located at the cell surface involved in the reduction of the natural water insoluble electron acceptor, Fe(III).

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.365-367
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• 1999

A fuel cell type biosensor for lactate was developed using a metal-reducing bacterium, Shewanella putrefaciens IR-1. Under the operational conditions, the bacterial cell suspension generated the current without an electrochemical mediator in the presence of lactate. The current was proportional to the lactate concentration up to 30 mM.

• Journal of Microbiology
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• 제37권4호
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• pp.206-212
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• 1999

In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various patty fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity, Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III), The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identified as Shewanella putrefaciens IR-1.

• Journal of Microbiology
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• 제38권3호
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• pp.180-182
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• 2000

The inhibitory effects of nitrate on Fe(III) and humic acid reduction were examined in Shewanella putrefaciens DK-1. Therer is no difference in Fe(III) reduction until 25 hours between cultures using Fe(III) production was decreased drastically when Fe(III) and nitrate were used as electron acceptors. The production of AHQDS(2,6-anthrahydroquinon disulfonate) showed similar patterns when AQDS alone and both AQDS and Fe(III) were used as electron acceptors. When AQDS(2,6-anthraquinon disulfonate) and nitrate were used as electron acceptors, the production of AHQDS was completely inhibited.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.132.1-132
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• 2000

No Abstract, See Full Text

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.127-133
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• 2006

Shewanella oneidensis MR-1 has the ability to inhale certain metals and chemical compounds and exhale these materials in an altered state; as a result, this microorganism has been widely applied in bioremediation protocols. However, the relevant characteristics of cell growth and biosynthesis of PuFAs have yet to be thoroughly investigated. Therefore, in this study, we have attempted to characterize the growth and fatty acid profiles of S. oneidensis MR-1 under a variety of temperature conditions. The fastest growth of S. oneidensis MR-1 was observed at $30^{\circ}C$, with a specific growth rate and doubling time of $0.6885h^{-1}\;and\;1.007 h$. The maximum cell mass of this microorganism was elicited at a temperature of $4^{\circ}C$. The eicosapentaenoic acid (EPA) synthesis of S. oneidensis MR-1 was evaluated under these different culture temperatures. S. oneidensis MR-1 was found not to synthesize EPA at temperatures in excess of $30^{\circ}C$, but was shown to synthesize EPA at temperatures below $30^{\circ}C$. The EPA content was found to increase with decreases in temperature. We then evaluated the EPA biosynthetic pathway, using a phylogenetic tree predicted on 16s rRNA sequences, and the homology of ORFs between S. oneidensis MR-1 and Shewanella putrefaciens SCRC-2738, which is known to harbor a polyketide synthase (PKS)-like module. The phylogenetic tree revealed that MR-1 was very closely related to both Moritella sp., which is known to synthesize DHA via a PKS-like pathway, and S. putrefaciens, which has been reported to synthesize EPA via an identical pathway. The homology between the PKS-like module of S. putrefaciens SCRC-2738 and the entire genome of S. oneidensis MR-1 was also analyzed, in order to mine the genes associated with the PKS-like pathway in S. oneidensis MR-1. A putative PKS-like module for EPA biosynthesis was verified by this analysis, and was also corroborated by the experimental finding that S. oneidensis MR-1 was able to synthesize EPA without the expression of $dihomo-{\gamma}-linoleic$ acid (DGLA) and arachidonic acid (AA) formed during EPA synthesis via the FAS pathway.

• 한국해양생명과학회지
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• 제1권1호
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• pp.18-24
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• 2016

Since April 2012, doctor fish in the breeding tank and in the quarantine tank in Hanwha Aquaplanet Yeosu Aquarium have been dying, accompanied by diffuse bleeding around the mouth, in the chin, and at the bottom of the abdomen. In this study, the cause of death would be examined through the bacteriological study of doctor fish and the rearing water quality in the aquarium. The water quality and the bacterial counts of the rearing water in the exhibit tank and in the quarantine tank were analyzed once a week, starting from August to November 2014. Water quality was measured based on the following data: temperature was in the range of 24.5~26.8℃, pH at 6.77~7.94, DO at 6.15~8.61 ppm, ammonia at 0~0.93 ppm, nitrite at 0.009~0.075 ppm, and nitrate at 1.1~40.9 ppm. Studies revealed that the differences in these water quality factors were not related to the death of doctor fish. Bacterial counts in the rearing waters of Garra rufa slightly increased to 10³~10⁴ CFU/ml, just before the death of the doctor fish. Twelve strains of bacteria were isolated from the dead fish and rearing waters. The isolates were identified as Aeromonas veronii, Citrobacter freundii, Pseudorhodoferax aquiterrae, Shewanella putrefaciens, and Vibrio anguillarum on the basis of 16S rRNA gene sequences. The most dominant species was C. freundii, which showed medium sensitivity to florfenicol and norfloxacin, and was resistant to amoxacillin, doxycycline, oxytetracycline, tetracycline, and trimethoprim. Ten isolates were confirmed to be pathogenic to the doctor fish. Doctor fish infected with C. freundii and S. putrefaciens showed high mortality in the experimental groups. These results indicate that the variation in bacterial numbers in the rearing water was related to the death of doctor fish. C. freundii and S. putrefaciens were directly implicated in causing the death of doctor fish in the aquarium.

• KSBB Journal
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• 제14권6호
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• pp.731-736
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• 1999

동두천과 반월공단에서 87가지의 균주를 분리하였다. 그 중 5가지 균주가 색도 제거율이 우수함을 보였으며, 동정을 통해 Shewanella putrefaciens, Aeromonas salmonicida(서로 다른 3종), Pseudomonas vesicularis로 각각 동정되었다. 동정된 5종의 미생물들은 성장에 최적인 pH와 온도는 각각 7.0과 30$^{\circ}C$이었으며, Gram negative, catalase nagative, rod shape, 그리고 비운동성을 보였다. 500 mL 플라스크에서 분리된 균주 중 단일균주를 사용하여 염색폐수를 처리한 경우 약 35%의색도 제거율을 보였다. 2균주를 조합하여 적용하였을 때는, 색도 제거율이 65%까지 증가한 반면, 3균주 조합 혹은 그 이상의 균주 조합에서는 2균주 조합보다 더 좋은 효율은 보이지 않았다. 염색폐수 분해시 $Mn^{2+},\;Fe^{3+}$와 같은 중금속의 영향을 조사하였는데, 70 ppm 이하의 농도에서는 별로 영향을 주지 않았다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제16권6호
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• pp.58-65
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• 2011

The bacterial uranium(VI) reduction and its resultant low solubility make this process an attractive option for removing U from groundwater. An impact of aqueous suspending iron phase, which is redox sensitive and ubiquitous in subsurface groundwater, on the U(VI) bioreduction by Shewanella putrefaciens CN32 was investigated. In our batch experiment, the U(VI) concentration ($5{\times}10^5M$) gradually decreased to a non-detectable level during the microbial respiration. However, when Fe(III) phase was suspended in solution, bioreduction of U(VI) was significantly suppressed due to a preferred reduction of Fe(III) instead of U(VI). This shows that the suspending amorphous Fe(III) phase can be a strong inhibitor to the U(VI) bioreduction. On the contrary, when iron was present as a soluble Fe(II) in the solution, the U(VI) removal was largely enhanced. The microbially-catalyzed U(VI) reduction resulted in an accumulation of solid-type U particles in and around the cells. Electron elemental investigations for the precipitates show that some background cations such as Ca and P were favorably coprecipitated with U. This implies that aqueous U tends to be stabilized by complexing with Ca or P ions, which easily diffuse and coprecipitate with U in and around the microbial cell.