• Restorative Dentistry and Endodontics
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• v.14 no.1
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• pp.57-70
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• 1989

The purpose of this study was to investigate the effect of salivary gland on the calcification of dentin in rats. 80 Sprague-Dawley male rats that weighed approximately 120gm were used in this study. 5 rats among them were shared as controls. 75 rats received sialoadenectomy were divided into submaxillary adenectomy group, parotidectomy group, and submaxillary-parotid gland combined removal group. In experimental groups, 25 rats in each of the 3 groups were sacrificed at the following intervals; 3 days, 1, 2, 3 and 4 weeks. All animals were sacrificed by vascular perfusion with 10% formalin. The maxillary incisors including periapical tissues were removed and defatted in 20% KOH solution at $0^{\circ}C$ for 24 hours, and dehydrated with acetone. Each tooth specimen was attached on the stab for scanning electron microscopic study. Gold was coated on the each specimen in the thickness of 300${\AA}$ at D.C. 1400V, 6mA for 6 minutes with coating machine (Eiko IB-3). Inner dentinal surfaces of the specimens were observed with SEM (Hitachi S-450). The results were as follows, 1. Parotidectomy groups were found to be inhibited the formation of dentinal calcification compared to submaxillary adenectomy groups in the eady stages. 2. Combined removal of submaxillary and parotid gland was appeared to cause more severe inhibition effect on the dentinal calcification than that of each salivary gland separately. 3. Inhibition of the calcification and mineralization of dentin caused by sialoadenectomy was more extreme from 3 day to 2 weeks after beginning of the experiments. However it was tended to be normalized after that. 4. Salivary gland was responsible for alterations in calcification and mineralization of dentinal growth.

• The Journal of the Korea Contents Association
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• v.9 no.3
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• pp.175-184
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• 2009

Korea shared 2.4% of world culture contents market in 2006 and ranked 9th. Therefore Korean government classified culture contents industry as a new growth-driving industries and started fixing the total contents policy promote system, exterminating illegal copying, protect copyright,improving contents creativity power, and supporting foreign market pioneering. Thus the importance of culture contents industry is increasing day by day. This study analyze the amount changes about production-inducing effect, value-added-inducing effects, employ-inducing effect, supply shortage effect and sectoral price effect, using inter-industry analysis according to time series. Especially, the sectoral price effect of culture contents industry increased by time pass. Thus, the influence of price changes in the culture contents industry to the other industries are increasing.

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Journal of Information Technology Services
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• v.16 no.3
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• pp.167-183
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• 2017

The rapid growth of information technology and mobile service platforms, i.e., internet, google, and facebook, etc. has led the abundance of data. Due to this environment, the world is now facing a revolution in the process that data is searched, collected, stored, and shared. Abundance of data gives us several opportunities to knowledge discovery and data mining techniques. In recent years, data mining methods as a solution to discovery and extraction of available knowledge in database has been more popular in e-commerce service fields such as, in particular, movie recommendation. However, most of the classification approaches for predicting the movie popularity have used only several types of information of the movie such as actor, director, rating score, language and countries etc. In this study, we propose a classification-based support vector machine (SVM) model for predicting the movie popularity based on movie's genre data and social network data. Social network analysis (SNA) is used for improving the classification accuracy. This study builds the movies' network (one mode network) based on initial data which is a two mode network as user-to-movie network. For the proposed method we computed degree centrality, betweenness centrality, closeness centrality, and eigenvector centrality as centrality measures in movie's network. Those four centrality values and movies' genre data were used to classify the movie popularity in this study. The logistic regression, neural network, $na{\ddot{i}}ve$ Bayes classifier, and decision tree as benchmarking models for movie popularity classification were also used for comparison with the performance of our proposed model. To assess the classifier's performance accuracy this study used MovieLens data as an open database. Our empirical results indicate that our proposed model with movie's genre and centrality data has by approximately 0% higher accuracy than other classification models with only movie's genre data. The implications of our results show that our proposed model can be used for improving movie popularity classification accuracy.

• Fisheries and Aquatic Sciences
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• v.19 no.8
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• pp.33.1-33.7
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• 2016

Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

• Journal of Microbiology and Biotechnology
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• v.20 no.5
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• pp.893-903
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• 2010

A cellulolytic and xylanolytic enzyme complex-producing alkalothermoanaerobacterium strain, Tepidimicrobium xylanilyticum BT14, is described. The cell was Grampositive, rod-shaped, and endospore-forming. Based on 16S rRNA gene analysis and various lines of biochemical and physiological properties, the strain BT14 is a new member of the genus Tepidimicrobium. The strain BT14 cells had the ability to bind to Avicel, xylan, and corn hull. The pH and temperature optima for growth were 9.0 and $60^{\circ}C$, respectively. The strain BT14 was able to use a variety of carbon sources. When the bacterium was grown on corn hulls under an anaerobic condition, a cellulolytic and xylanolytic enzyme complex was produced. Crude enzyme containing cellulase and xylanase of the strain BT14 was active in broad ranges of pH and temperature. The optimum conditions for cellulase and xylanase activities were pH 8.0 and 9.0 at $60^{\circ}C$, respectively. The crude enzyme had the ability to bind to Avicel and xylan. The analysis of native-PAGE and native-zymograms indicated the cellulosebinding protein showing both cellulase and xylanase activities, whereas SDS-PAGE zymograms showed 4 bands of cellulases and 5 bands of xylanases. Evidence of a cohesinlike amino acid sequence seemed to indicate that the protein complex shared a direct relationship with the cellulosome of Clostridium thermocellum. The crude enzyme from the strain BT14 showed effective degradation of plant biomass. When grown on corn hulls at pH 9.0 and $60^{\circ}C$ under anaerobic conditions, the strain BT14 produced ethanol and acetate as the main fermentation products.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.3
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• pp.181-189
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• 2015

As a study on entrepreneurship and venture companies to support, this research is to find how to inflow the fund which is provided by private companies. This is to propose a shared platform for Information for connecting producers and consumers, corporate investors to help fund inflows to private companies and utilization of the enterprise information collected by government. Entrepreneurship support policies, as one of economy activation have a limit in the size of the support of the government therefore support continued growth through aggressive inflow of private funds is needed. It is significant to provide the environment that private funds could spill into the environment to provide for excellent start-up companies.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.3
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• pp.493-500
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• 2015

Personal information exposed in the Internet is increasing by the public data opening and sharing, vitalization of SNS(Social Network Service) and growth of information shared between users. Exposed personal information in the Internet can infringe upon targeted users using linkage attack or background attack. To prevent these attack De-identification models were appeared a few years ago. The 'k-anonymity' has been introduced in the first place, and the '${\ell}$-diversity' and 't-closeness' have been followed up as solutions, and diverse algorithms have been being suggested for performance improvement nowadays. However, industry or public sectors actually needs a whole solution as a system for the de-identification process rather than performance of the de-identification algorithm. This paper explains a way of de-identification techique for 'k-anonymity', '${\ell}$-diversity', and 't-closeness' algorithm using QI(Quasi-Identifier) grouping method in the relational database.

• Korean Institute of Interior Design Journal
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• v.24 no.5
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• pp.117-127
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• 2015

This paper depicts a case study of the BIM (Building Information Modeling)-enabled evaluation of design alternatives for an actual remodeling project in Yeongwol, Korea. The increase of urban population and income followed by the growth of economy has derived massive supply of grand scale housing project in Korea since 1970s. Consequently, building remodeling became one of the feasible resolutions for renovating such old housings in these days. This paper aims to introduce a technical approach to such remodeling projects based on BIM-enabled applications focusing on quantitative analysis of design alternatives. Among the technical issues of such building remodeling projects, this paper focuses on the BIM-enabled area analysis and comparison between design alternatives to support decision-making even in early phase of remodeling design process. BIM and its variety of applications have broadly influenced the domain of AEC-FM (Architecture, Engineering, Construction, and Facility Management) within the lifecycle of buildings. As one of the applications facilitated by BIM, the automated area calculation and scenario-based comparison between alternatives can play an important role in the early phase of remodeling project. We modeled three design alternatives (buildings) and three housing modules (units) based on the actual case in Yeongwol city, Korea using a BIM design authoring tool. Nine combinatorial BIM models were demonstrated for the BIM-enabled review process described in this paper. To determine the most optimal design scenario among nine alternatives, this paper demonstrates a result of the conducted spatial program review. The main subject includes specific spatial program issues on; 1) the number of unit spaces; and 2) area of individual/grouped and private/shared spaces.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.889-893
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• 2012

Appressorium is a specialized infection structure of filamentous pathogenic fungi and plays an important role in establishing a pathogenic relationship with the host. The Egh16/Egh16H family members are involved in appressorium formation and pathogenesis in pathogenic filamentous fungi. In this study, a homolog of Egh16H, Magas1, was identified from an entomopathogenic fungus, Metarhizium acridum. The Magas1 protein shared a number of conserved motifs with other Egh16/Egh16H family members and specifically expressed during the appressorium development period. Magas1-EGFP fusion expression showed that Magas1 protein was not localized inside the cell. Deletion of the Magas1 gene had no impact on vegetative growth, conidiation and appressorium formation, but resulted in a decreased mortality of host insect when topically inoculated. However, the mortality was not significant between the Magas1 deletion mutant and wild-type treatment when the cuticle was bypassed by injecting conidia directly into the hemocoel. Our results suggested that Magas1 may influence virulence by affecting the penetration of the insects' cuticle.