• The Plant Pathology Journal
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• v.24 no.1
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• pp.17-23
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• 2008

A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.

• ALGAE
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• v.26 no.4
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• pp.289-297
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• 2011

Ceramium riosmenae sp. nov. is described from Baja California Sur, Mexico based on morphological and molecular data. The new species is characterized by erect thalli only, penetrating rhizoids on Gracilaria, 7-8 periaxial cells, five cortical initials per periaxial cell, complete cortication throughout, an average of 11-12 segments between branching points, rare adventitious branchlets, and cruciate tetrasporangia. Although C. riosmenae sp. nov. is similar to C. interruptum, C. sinicola, and C. codicola reported from Baja California Sur, Mexico in size and habit, it differs from these species in the number of cortical initials, habit, degree of cortication, host, and the shape of rhizoidal tips. C. riosmenae is separated from C. interruptum with interrupted cortication and four cortical initials from C. sinicola with spins near the apex and incomplete cortication near the base and from C. codicola with bulbous rhizoids on Codium. Our rbcL sequences reveal sufficient sequence divergence (2.4-3.9%) between C. riosmenae and C. interruptum, C. sinicola, and C. codicola to warrant species recognition and to separate C. riosmenae from these species on a phylogenetic tree.

• Journal of Korea Multimedia Society
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• v.5 no.5
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• pp.552-561
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• 2002

In this paper, a new watermarking algorithm for STL files which contains 3D geometric information as triangular facets is proposed. STL files are widely used in rapid prototyping industry as a standard interchange format. The proposed algorithm inserts multi-bit watermark information into the surface normal vector and vertex description area of STL file without distorting the original 3D geometric information. According to the watermark bits, the position of normal vector and the direction of vertex sequence are modulated. The proposed algorithm is robust to the attack of changing the order of the triangular meshes. In addition, the invisibility requirement is also satisfied. Experiment results show that the proposed algorithm can encode and decode watermark bits into the various STL files without any distortion of 3D shape.

• Korean Journal of Computational Design and Engineering
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• v.9 no.1
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• pp.51-61
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• 2004

Mechanical parts are often grouped into part families based on the similarity of their shapes, to support efficient manufacturing process planning and design modification. The 2-part sequence papers present similarity assessment techniques to support part family classification for machined parts. These exploit the multiple feature decompositions obtained by the feature recognition method using convex decomposition. Convex decomposition provides a hierarchical volumetric representation of a part, organized in an outside-in hierarchy. It provides local accessibility directions, which supports abstract and qualitative similarity assessment. It is converted to a Form Feature Decomposition (FFD), which represents a part using form features intrinsic to the shape of the part. This supports abstract and qualitative similarity assessment using positive feature volumes.. FFD is converted to Negative Feature Decomposition (NFD), which represents a part as a base component and negative machining features. This supports a detailed, quantitative similarity assessment technique that measures the similarity between machined parts and associated machining processes implied by two parts' NFDs. Features of the NFD are organized into branch groups to capture the NFD hierarchy and feature interrelations. Branch groups of two parts' NFDs are matched to obtain pairs, and then features within each pair of branch groups are compared, exploiting feature type, size, machining direction, and other information relevant to machining processes. This paper, the second one of the two companion papers, describes the similarity assessment method using NFD.

• Korean Journal of Computational Design and Engineering
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• v.9 no.1
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• pp.44-50
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• 2004

Mechanical parts are often grouped into part families based on the similarity of their shapes, to support efficient manufacturing process planning and design modification. The 2-part sequence papers present similarity assessment techniques to support part family classification for machined parts. These exploit the multiple feature decompositions obtained by the feature recognition method using convex decomposition. Convex decomposition provides a hierarchical volumetric representation of a part, organized in an outside-in hierarchy. It provides local accessibility directions, which supports abstract and qualitative similarity assessment. It is converted to a Form Feature Decomposition (FFD), which represents a part using form features intrinsic to the shape of the part. This supports abstract and qualitative similarity assessment using positive feature volumes. FFD is converted to Negative Feature Decomposition (NFD), which represents a part as a base component and negative machining features. This supports a detailed, quantitative similarity assessment technique that measures the similarity between machined parts and associated machining processes implied by two parts' NFDs. Features of the NFD are organized into branch groups to capture the NFD hierarchy and feature interrelations. Branch groups of two parts' NFDs are matched to obtain pairs, and then features within each pair of branch groups are compared, exploiting feature type, size, machining direction, and other information relevant to machining processes. This paper, the first one of the two companion papers, describes the similarity assessment methods using convex decomposition and FFD.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.81-85
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• 2002

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Stenotrophomonas, but were not included in these genera. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium. We attempted to screening of bioactive compounds from oligotrophs which was isolated from forest soil. The active compounds were analyzed by mass and NMR spectrum, one of them identified as crisamicin A. Another one designated as SAPH is a new compound. The results indicate that there were possibilities for finding new compounds from the rare microorganisms such as oligotrophs.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.12
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• pp.55-64
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• 2008

This paper describes a research work of developing an automated progressive design system for manufacturing the product with multi processes such as piercing, bending, and deep drawing. An approach to the system for progressive working is based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system consists of three main modules, which are shape treatment, strip layout, and die layout modules. Based on knowledge-based rules, the system is designed considering several factors, such as material and thickness of a product, piercing, bending and deep drawing sequence, and the complexities of the blank geometry and punch profiles. It generates the strip layout drawing for an automobile product. Die design for each process is carried out through the die layout module from the results of the strip layout module. Results obtained using the modules enable the designers for manufacturing products with multi processes to be more efficient in this field.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.9
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• pp.49-56
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• 2004

With the wide application of fiber-reinforced composite material in aero-structures and mechanical parts, the design of composite joint have become a very important research area because they are often the weakest areas in composite structures. In this paper, the linear finite element analyses in which the pin of the composite joint was assumed to be the frictionless rigid body were performed and predict the strength of the mechanically fastened composite joint using the failure area index method. By the failure area index method, the strength of the mechanically fastened composite joint which has the specimen of different shape, hole size and stacking sequence could be predicted within 12.2%.

• Geomechanics and Engineering
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• v.10 no.2
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• pp.175-188
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• 2016

The construction of a new cavern modifies the state of stresses and displacements in a zone around the existing cavern. For multiple caverns, the size of this influence zone depends on the ground type, the in situ stress, the cavern span and shape, the width of the pillar separating the caverns, and the excavation sequence. Performances of underground twin caverns can be unsatisfactory as a result of either instability (collapse) or excessive displacements. These two distinct failures should be prevented in design. This study simulated the ultimate and serviceability performances of underground twin rock caverns of various sizes and shapes. The global factor of safety is used as the criterion for determining the ultimate limit state and the calculated maximum displacement around the cavern opening is adopted as the serviceability limit state criterion. Based on the results of a series of numerical simulations, simple regression models were developed for estimating the global factor of safety and the maximum displacement, respectively. It was proposed that a proper pillar width can be determined based on the threshold influence factor value. In addition, design charts with regard to the selection of the pillar width for underground twin rock caverns under similar ground conditions were also developed.

• Mycobiology
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• v.28 no.1
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• pp.11-16
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• 2000

The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.