• Title/Summary/Keyword: Shape Criterion

Search Result 282, Processing Time 0.029 seconds

Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining (Hoechst 33258 Staining을 이용한 웅성 생쥐 성세포의 간편 분류)

  • Kim, Kyoung Guk;Park, Young Sik
    • Journal of Embryo Transfer
    • /
    • v.30 no.3
    • /
    • pp.213-218
    • /
    • 2015
  • In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming at experimental applications. For developing the efficient method to identify the stage of sperm cells, the morphological characteristics of sperm cells were investigated by staining the cells with blue fluorescent dye Hoechst 33258, and a criterion for male germ cell classification was elicited from results of the previous investigation, then the efficiency of the criterion was verified by applying it to assort the germ cells recovered from male mice in age from 6 to 35 days. As morphological characteristics, spermatogonia significantly differed from spermatocytes in size, appearance and fluorescent patches of nucleus, and spermatids could also be distinguished from spermatozoa by making a difference in the volume and shape of nucleus and the shape and fluorescence of tail. Aforesaid criterion was applicable for classifying in vitro cultured sperm cells by verifying its efficiency and propriety for assorting the stages of testicular germ cells. However, the fluorescent staining showed that germ cells in mouse testis should be dramatically differentiated and developed at 21 days and 35 days of age, which were known as times of sexual puberty and maturity in male mice, respectively. In conclusion, the results indicated that this simple criterion for sperm cell classification using fluorescence staining with Hoechst 33258 may be highly efficient and reasonable for spermatogenesis study.

Stability Analysis for Ground Uplift in Underground Storage Caverns for High Pressurized Gas using Hoek-Brown Strength Criterion and Geological Strength Index (GSI) (Hoek-Brown 강도기준식 및 암질강도지수를 이용한 고압 유체 지하저장 공동의 융기에 대한 안정성 평가)

  • Kim, Hyung-Mok
    • Tunnel and Underground Space
    • /
    • v.24 no.4
    • /
    • pp.289-296
    • /
    • 2014
  • A simple analytical approach for stability assessment of underground storage caverns against ground uplift of overburden rock above the rock caverns for high pressurized fluid such as compressed air energy storage (CAES) and compressed natural gas (CNG) was developed. In the developed approach, we assumed that failure plane of the overburden is straight upward to ground surface, and factor of safety can be calculated from a limit equilibrium analysis in terms of this cylindrical shape failure model. The frictional resisting force on the failure plane was estimated by Hoek-Brown strength criterion which replaces with Mohr-Coulomb criterion such that both intact rock strength and rock mass conditions can be considered in the current approach. We carried out a parametric sensitivity analysis of strength parameters under various rock mass conditions and demonstrated that the factor of safety againt ground uplift was more sensitive to Mohr-Coulomb strength criterion rather than Hoek-Brown criterion.

Shape Decision of the Stainless Steel at the Skin Pass Mill (스테인리스 냉연공정에서 강판의 형상판정)

  • Hur, Yone-Gi;Choi, Young-Kiu
    • Proceedings of the IEEK Conference
    • /
    • 2008.06a
    • /
    • pp.1047-1048
    • /
    • 2008
  • The objective of this paper is to build a decision method of the shape quality for the stainless steel and ensure reducibility of working-load for next rolling process. The criterion of the shape quality is derived from the customer needs, and Automatic Shape Decision System is implemented in SPM (Skin Pass Mill). The methods of shape decision are based on curve fitting and frequency analysis and so on. The Field Test with concerned customers is successful. The performance of right decision is 99% and the claims from the customer have been largely reduced.

  • PDF

The Sectional Analysis of Trunk-lid using the Equilibrium Approach and Three-Dimensional Shape Composition (평형해법을 이용한 트렁크 리드의 단면해석과 3차원 형상합성)

  • 정동원
    • Journal of Ocean Engineering and Technology
    • /
    • v.15 no.2
    • /
    • pp.66-71
    • /
    • 2001
  • A sectional analysis of trunk-lid carried out by using the equilibrium approach based on the force balance together with geometric relations and plasticity theory. In computing a force balance equation, it is required to define a geometric curve approximating the shape of sheet metal at any step of deformation from the interaction between the die and the deformed sheet. The trunk-lid panel material is assumed to possess normal anisotropy and to obey Hill's new yield criterion. Deformation of each section of trunk-lid panel is simulated and composed to get the three-dimensional shape by using CAD technique. It was shown that the three-dimensional shape composition of the two-dimensional analysis.

  • PDF

Hypotheses Testing for the Shape Parameter of the Weibull Lifetime Data

  • Kang, Sang-Gil;Kim, Dal-Ho;Cho, Jang-Sik
    • Journal of Korean Society for Quality Management
    • /
    • v.27 no.4
    • /
    • pp.153-166
    • /
    • 1999
  • In this paper, we address the Bayesian hypotheses testing for the shape parameter of weibull model. In Bayesian testing problem, conventional Bayes factors can not typically accommodate the use of noninformative priors which are improper and are defined only up to arbitrary constants. To overcome such problem, we use the recently proposed hypotheses testing criterion called the intrinsic Bayes factor. We derive the arithmetic and median intrinsic Bayes factors and use these results to analyze real data sets.

  • PDF

Shape Similarity Analysis for Verification of Hazard Map for Storm Surge : Shape Criterion (폭풍해일 침수예상도 검증을 위한 형상유사도 분석 : 형상기준)

  • Kim, Young In;Kim, Dong Hyun;Lee, Seung Oh
    • Journal of Korean Society of Disaster and Security
    • /
    • v.12 no.3
    • /
    • pp.13-24
    • /
    • 2019
  • The concept of shape similarity has been applied to verify the accuracy of the SIND model, the real-time prediction model for disaster risk. However, the CRITIC method, one of the most widely used in geometric methodology, is definitely limited to apply to complex shape such as hazard map for coastal disaster. Therefore, we suggested the modified CRITIC method of which we added the shape factors such as RCCI and TF to consider complicated shapes. The matching pairs were manually divided into exact-matching pairs and mis-matching pairs to evaluate the applicability of the new method for shape similarity into hazard maps for storm surges. And the shape similarity of each matching pair was calculated by changing the weights of each shape factor and criteria. Newly proposed methodology and the calculated weights were applied to the objects of the existent hazard map and the results from SIND model. About 90% of exact-matching pairs had the shape similarity of 0.5 or higher, and about 70% of mis-matching pairs were it below 0.5. As future works, if we would calibrate narrowly and adjust carefully multi-objects corresponding to one object, it would be expected that the shape similarity of the exact-matching pairs will increase overall while it of the mis-matching pairs will decrease.

Damage Detection of a Steel Member Using Modal Testing (강부재의 손상발견을 위한 모달실험 기법)

  • Jang, Jeong Hwan;Lee, Jung Whee;Kim, Sung Kon;Chang, Sung Pil
    • Journal of Korean Society of Steel Construction
    • /
    • v.9 no.4 s.33
    • /
    • pp.467-477
    • /
    • 1997
  • A series of experimental tests have been performed on a tube beam in which artificial damage is applied in order to address damage detectability using modal analysis. Modal parameters considered are frequency, displacement mode shape and strain mode shape CoMAC(Coordinate Modal Assurance Criterion) and Modal Vector Error have been adopted for presenting the change of displacement mode shape and strain mode shape. It is revealed strain mode shape is the most sensitive to damage.

  • PDF

Analysis of cavity expansion based on general strength criterion and energy theory

  • Chao Li;Meng-meng Lu;Bin Zhu;Chao Liu;Guo-Yao Li;Pin-Qiang Mo
    • Geomechanics and Engineering
    • /
    • v.37 no.1
    • /
    • pp.9-19
    • /
    • 2024
  • This study presents an energy analysis for large-strain cavity expansion problem based on the general strength criterion and energy theory. This study focuses on the energy dissipation problem during the cavity expansion process, dividing the soil mass around the cavity into an elastic region and a plastic region. Assuming compliance with the small deformation theory in the elastic region and the large deformation theory in the plastic region, combined with the general strength criterion of soil mass and energy theory, the energy dissipation solution for cavity expansion problem is derived. Firstly, from an energy perspective, the process of cavity expansion in soil mass is described as an energy conversion process. The energy dissipation mechanism is introduced into the traditional analysis of cavity expansion, and a general analytical solution for cavity expansion related to energy is derived. Subsequently, based on this general analytical solution of cavity expansion, the influence of different strength criterion, large-strain, expansion radius, cavity shape and characteristics of soil mass on the stress distribution, displacement field and energy evolution around the cavity is studied. Finally, the effectiveness and reliability of theoretical solution is verified by comparing the results of typical pressure-expansion curves with existing literature algorithms. The results indicate that different strength criterion have a relatively small impact on the displacement and strain field around the cavity, but a significant impact on the stress distribution and energy evolution around the cavity.

Design of Step-Stress Accelerated Life Tests for Weibull Distributions with a Nonconstant Shape Parameter

  • Kim, C. M.;D. S. Bai
    • Journal of the Korean Statistical Society
    • /
    • v.28 no.4
    • /
    • pp.415-433
    • /
    • 1999
  • This paper considers the design of step-stress accelerated life tests for the Weibull distribution with a nonconstant shape parameter under Type I censoring. It is assumed that scale and shape parameters are log-linear functions of (possibly transformed) stress and that a cumulative exposure model holds for the effect of changing stress. The asymptotic variance of the maximum likelihood estimator of a stated quantile at design stress is used as an optimality criterion. The optimum three step-stress plans are presented for selected values of design parameters and the effects of errors in pre- estimates of the design parameters are investigated.

  • PDF

Variation of Notch Shape on the Delamination Zone Behavior in Al/AFRP Laminates (노치형태 변화에 따른 Al/AFRP 적층재의 층간분리거동)

  • Song, Sam-Hong;Kim, Cheol-Woong
    • Proceedings of the KSME Conference
    • /
    • 2001.11a
    • /
    • pp.278-285
    • /
    • 2001
  • Aluminum/Aramid Fiber Reinforced Plastic(Al/AFRP) laminates are applied to the fuselage-wing intersection. The main objective of this study was to evaluate the delamination zone behavior of Al/AFRP with a saw-cut and circular hole using average stress criterion and the effect of notch geometry. Mechanical tests were carried out to determine the cyclic-bending moment and delamination zone observed ultrasonic C-scan pictures. In case of Al/AFRP containing saw-cut specimen, the shape and size of the delamination zone formed along the fatigue crack. However, in case of Al/AFRP containing circular hole specimen, the shape and size of delamination zone formed two types. first type, delamination zone formed along the fatigue crack. Second type, not observed fatigue crack. Therefore, delamination zone was formed dependently of the circular hole shape.

  • PDF