• 제목/요약/키워드: Shannon species diversity index

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Bacterial Community and Diversity from the Watermelon Cultivated Soils through Next Generation Sequencing Approach

  • Adhikari, Mahesh;Kim, Sang Woo;Kim, Hyun Seung;Kim, Ki Young;Park, Hyo Bin;Kim, Ki Jung;Lee, Youn Su
    • The Plant Pathology Journal
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    • 제37권6호
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    • pp.521-532
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    • 2021
  • Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

구조다양성 표현인자 연구를 통한 내설악 전나무 고목림 (자연림) 구조 조사 (A Study on the Stand Structure of Korean Fir Natural Forest in Naesorak through the Investigation of Stand Structure Diversity Features)

  • 윤영일
    • 환경생물
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    • 제29권4호
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    • pp.345-352
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    • 2011
  • 내설악 전나무 자연림 임분구조를 좀 더 체계적으로 이해하기 위하여 종다양성 추정에 사용되는 Shannon-Weaver-Index를 응용한 Herles의 구조표현인자 다양성 계산 방법을 도입하여 12개의 임분을 조사하였다. 임분 구조 변화는 당연히 phase의 변화에 종속되지만 개개인자들 간의 연관에서 자연림의 특성이 재확인되었다. 우선 내설악 전나무 자연림의 구조는 표현인자 다양성의 결과에서 보이는 것처럼 다양성이 상대적으로 낮은 것으로 나타났다. 인자 간의 상관에서 종다양성은 구조 변화 관련 전체 인자 (다양성)와 미약한 관계만 보이며, 어느 정도 설명이 가능한 연관을 보이는 것으로는 개체수였으며, 개체수가 증가할수록 임분구조 전체의 다양성은 떨어지는 결과를 보인다. 입목축적이나 고사목 축적은 대부분 조사구에서 천연갱신고도와 연관을 보이며 다른 인자와는 별다른 관계를 보이지 않는 것으로 나타났다. 타 구조표현인자 서로서로의 연관은 천연갱신고도와 관련하여 울폐도나 수관면적, 형태 등과 연관을 보였으며 수고나 흉고직경은 타 인자들과 별다른 연관을 보이지 않았다. 전체적으로 내설악 전나무림 구조변화 이해에 도움을 주는 결과를 가져왔지만 좀 더 많은 조사구에 대한 조사가 필요하리라 본다.

안면도 먹넌출 집단의 유전다양성과 공간적 유전구조 (Genetic Diversity and Spatial Genetic Structure of Berchemia racemosa var. magna in Anmyeon Island)

  • 송정호;임효인;장경환;홍경낙;한진규
    • 원예과학기술지
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    • 제32권1호
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    • pp.84-90
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    • 2014
  • 우리나라에서 먹넌출은 안면도 지역에서만 소나무 숲에서 제한적으로 분포하는 덩굴성 식물이다. 본 연구는 먹넌출 집단의 분포형태와 특성, 유전다양성 및 공간분포에 따른 유전구조를 파악하는데 있다. 선발된 8개 I-SSR primer에서 총 50개의 I-SSR 증폭산물을 얻었으며 37개의 단형성 증폭산물을 제외한 13개의 다형적 증폭산물을 분석에 이용하였다. 공간적 자기상관 분석을 위한 조사구 $90m{\times}70m$내에 총 39개체의 먹넌출이 자생하고 있었으며, 군집지수(aggregation index)는 0.706으로 집중분포(clumped distribution)하는 공간분포를 나타냈다. I-SSR 표지자 분석 결과 39개체 중 유전자형이 서로 다른 21개의 유성생식체(genet)가 식별되었으며, 유전자형 비율(G/N)은 53.8%, 유전자형 다양성(D)은 0.966, 유전자형 균등도(E)는 0.946으로 각각 나타났다. Shannon의 다양성지수(I = 0.598)는 적은 개체수와 제한적 분포에도 불구하고 다른 수종들에 비해 비교적 높은 유전다양성을 나타냈다. Tanimoto distance를 이용한 공간적 자기상관 분석 결과 안면도 먹넌출의 현지외 보존을 위한 표본 추출 전략은 6m 이상의 간격을 두고 개체를 선발하는 것이 타당한 것으로 나타났다.

하천환경의 생물학적 평가를 위한 간이저서동물지수(SBMI)의 개발 (Development of Simple Benthic Macroinvertebrates Index (SBMI) for Biological Assessment on Stream Environment)

  • 공동수;민정기;노성유
    • 한국물환경학회지
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    • 제34권5호
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    • pp.514-536
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    • 2018
  • GPI (Group Pollution Index) using 29 indicator groups of Korean benthic macroinvertebrates was proposed in 1992, a higher category taxa-level index developed for rapid field assessment of organic water pollution. This study was performed to revise the assessment scheme of GPI based on taxonomic performance and ecological information accumulated since 1992. The original GPI was renamed SBMI (Simple Benthic Macroinvertebrates Index), and SBMI was based on saprobic valency of 26 indicator groups composed of higher category taxa (mainly family ~ phylum) excluding some genus or species-level taxa. SBMI revealed highly significant correlation with concentration of 5-day biochemical oxygen demand ($BOD_5$) (correlation coefficient r = 0.78, n = 569 sites), total suspended solids (r = 0.69), and total phosphorus (r = 0.77). Also, SBMI revealed strong correlation with Shannon-Weaver's species diversity (r = -0.85), Margalef's species richness (r = -0.85), and McNaughton's dominance (r = 0.83). Determination coefficient of SBMI to concentration of water quality items and values of community indices such as species diversity was 3 ~ 8 % and approximately 11 ~ 14 % higher than that of GPI, respectively. Correlation between SBMI and water quality factors or community indices such as species diversity did not reveal much difference compared to that of species-level indices, such as BMI (Benthic Macroinvertebrates Index) and ESB (Ecological Score of Benthic Macroinvertebrates). SMBI is a simple-qualitative index with higher category taxa easily identified, and is applicable for rapid field assessment of water environment impairment.

강원 중부지역 산림 내 나비류 분포에 관한 연구(I) (The Study on the Distribution of Lepidoptera on forest stand in Central Gangwon Area(I))

  • 장석준;심상준;최종운;원대성;김종국
    • Journal of Forest and Environmental Science
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    • 제21권1호
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    • pp.66-74
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    • 2005
  • 본 연구는 2004년 7월과 8월에 백두대간에 서식하는 나비목 가운데 나방류의 곤충상을 조사하였다. 조사결과 총 채집종은 14과 162종 570개체이었으며, 한계리가 12과 69종 149개체로 가장 많이 채집되었고, 진부리가 6과 12종 58개체로 가장 적게 채집되었다. 채집종중 우점을 나타낸 종은 솔송나방 40개체, 머루박각시 32개체, 애물결박각시 27개체, 물결멧누에나방 26개체, 꽃술재주나방 23개체 순으로 조사되었다. Shannon(H') 지수 및 균등도지수(J')는 봉명리 1.34, 0.86, 감정리 1.48, 0.89, 한계리 1.77, 0.94, 용대리 1.53, 0.93, 귀둔리 1.56, 0.94, 진부리 1.15, 0.83로 조사되었으며, 유사도지수(QS)는 진부리와 귀둔리가 0.11로 낮았고, 용대리와 한계리가 0.38로 가장 높았다.

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Phytosociological Analysis of Woody Species in Kedarnath Wildlife Sanctuary and Its Adjoining Areas in Western Himalaya, India

  • Malik, Zubair A.;Bhatt, A.B.
    • Journal of Forest and Environmental Science
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    • 제31권3호
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    • pp.149-163
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    • 2015
  • The aim of the present study was to assess the variation in species composition and diversity of woody species at different altitudes (900 to 2600 m asl) in Kedarnath Wildlife Sanctuary (KWLS) and its adjoining areas in Garhwal Himalaya, India. A total of 94 woody plant species (including 44 tree and 50 shrub species) belonging to 72 genera and 44 families were reported. Density varied from $235{\pm}9$ to $505{\pm}21trees\;ha^{-1}$ and $4,730{\pm}474$ to $9,530{\pm}700shrubs\;ha^{-1}$. Total basal cover varied from $10.49{\pm}0.66$ to $42.92{\pm}2.57m^2ha^{-1}$ (trees) and $0.36{\pm}0.024$ to $0.62{\pm}0.047m^2ha^{-1}$ (shrubs). Shannon-Wiener Index fluctuated between 2.30 to 3.53 (trees) and 2.74 to 3.78 (shrubs). Analysis of variance (ANOVA) indicated that altitude and aspect had significant effect on the distribution of woody species. Taxonomically, Rosaceae with 15 species emerged as the dominant family. Low value of maturity index and contiguous distribution of species denoted the early successional status of the studied forests. The conservation assessment based on altitudinal regimes and the information on species structure and function can provide baseline information for monitoring and sustaining the biodiversity.

강원 중부지역 산림 내 나비류 분포에 관한 연구(II) (Distribution of Lepidoptera on Forest Stand in Central Gangwon Area(II))

  • 장석준;최종운;심상준;김종국
    • Journal of Forest and Environmental Science
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    • 제22권1호
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    • pp.58-65
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    • 2006
  • 본 연구는 백두대간에 서식하는 나비목 곤충의 분포를 파악하기 위하여 2005년 7월과 8월에 수행되었다. 총 15과 123속 462개체가 채집되었으며, 점봉산(인제군 인제읍 귀둔리) 15과 63속 166개체, 응복산(홍천군 내면 명계리) 15과 65 속 159개체, 오대산(평창군 도암면 병내리) 9과 40속 137개체가 채집되었다. 조사지역에서 채집된 종들 중 우점하고 있는 5종은 머루박각시 (Ampelophaga rubiginosa) 54개체, 참나무산누에나방 (Antheraea yamamai) 35개체, 솔송나방 (Dendrolimus superans) 27개체, 북방뒷노랑밤나방 (Triphaenopsis jezoensis) 14개체, 얼룩매미나방 (Lymantria monacha) 13개체였다. Shannon(H') 지수와 균등도지수 (J') 는 점봉산 1.52, 0.84, 응복산1.69, 0.91, 오대산 1.37, 0.83, 유사도지수 (QS)는 점봉산과 응복산이 0.28로 다소 높았으며, 점봉산과 오대산이 0.11로 낮게 나타났다.

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Assessment of the Distribution of the Street Trees of Suwon City for Biodiversity

  • Choi, Sun A;Kim, Shin Won
    • KIEAE Journal
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    • 제14권5호
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    • pp.49-57
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    • 2014
  • This research is about analysis and evaluation of biodiversity of Suwon's street tree, then understanding the problem of it and finally finding a solution. Because the increasing damage of the street trees by disease and insects, insecticide is applied to prevent further damage. However, this insecticide is found to be cancer genic and causing hygienic threat to civilians. Therefore, by gathering Suwon's internal statistics about Suwon's street tree, the trees are divided into three categories, tall evergreen trees, deciduous trees, shrubs following Frank's 30-20-10 theory(1990). Also, according to species diversity index, the problem of disease and insect is researched in terms of biodiversity, and here we suggests solutions to counter such problems. According to the results, the trees planted in Suwon was found to be 31 families, 43 genus and 58 species. The most used kinds, almost 85% of the whole species, are found to be Rhododendron indicum (L.) Sweet, Buxus koreana Nakai ex Chung & al, Euonymus japonicus Thunb, Ligustrum obtusifolium Siebold & Zucc. Besides these, the rest of 15% of street trees had little variety. Therefore, it is necessary to plant tree variously and equally in terms of biodiversity. If this Frank's 10-20-30 solution is not enough to completely solve coulure problem, then further research will be done on soil properties, and local features for improvement of Suwon street tress.

Tree Diversity, Distribution and Population Structure of a Riparian Forest from Certain Zones along the Dikhu River in Nagaland, India

  • Leishangthem, Devlin;Singh, Maibam Romeo
    • Journal of Forest and Environmental Science
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    • 제34권1호
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    • pp.31-45
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    • 2018
  • At present the biodiversity of the riparian forest is under threat due to various anthropogenic pressures. Hence study was conducted along the three unprotected zones of riparian forest in Dikhu river, Nagaland, north-east India in order to compared the diversity, distribution and population structure of riparian trees. In each zone $100{\times}100m^2$ plot were marked and subdivided into 20 plots of $10{\times}10m^2$. 10 plots on each side of the river were taken randomly covering 0.02 ha. Only tree with dbh ${\geq}10cm$ and dbh above 1.5 m above ground level were recorded as individual species. A total of 29 tree species belonging to 18 families were recorded from the three zones of the river. Tree species richness was highest at the middle zone (19) followed by upper (14) and lower zone (7). The most abundant species and family recorded at upper, middle and lower zones were Melia azaderach of Euphorbiaceae ($380\;stems\;ha^{-1}$), Terminalia chebula of Euphorbiaceae ($432.5\;stems\;ha^{-1}$) and Duabanga grandiflora of Lythraceae ($365\;stems\;ha^{-1}$) respectively. The ranges of diversity indices observed in the three zones were: Shannon-Wiener index (1.25-0.73), Simpson diversity index (0.42-0.93), Evenness index (0.47-0.37) and Index of co-dominance (0.75-0.94). Rest of other indices were also estimated and compared. Distribution of trees shows the contagious pattern common in the upper and middle zones and regular in the lower zone. The girth size class analysis demonstrated that the riparian forest is in less mature succession stage. This study emphasize the need for management and conservation of riparian forest by developing policy to declare the riparian zone as protected area to prevent further degradation and loss of biodiversity from these unregulated zones along the river.

순천만에 자생하는 염생식물 근권에서 유래한 해양세균의 계통학적 분석 및 다양성 (Phylogenetic Analysis and Diversity of Marine Bacteria Isolated from Rhizosphere Soils of Halophyte in Suncheon Bay)

  • 유영현;박종명;이명철;김종국
    • 한국미생물·생명공학회지
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    • 제43권1호
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    • pp.65-78
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    • 2015
  • 순천만의 염생식물 근권에서 정주하는 세균의 분리를 위해 순천만에서 우점하는 자생식물인 칠면초 군락 3개 지점을 선발하여 샘플링 하였다. 시료는 marine broth, tryptic soy broth 한천배지를 통해 분리되었으며, 형태학적인 구분을 통해 순수분리되었다. 분리주의 16S rDNA를 분석하여 총 92 균주가 동정되었다. 이들의 유연관계 확인을 위한 계통수 작성 결과, 각각 firmicutes (56.5%), gamma-proteobacteria (29.3%), alpha-proteobacteria (5.4%), actinobacteria (5.4%), bacteroidetes (3.3%)에 속하였다. Shannon’s Diversity index (H')를 산출하였을 때 각각 1.675, 1.924, 2.04로, 채취 지점별로 종 다양성의 차이를 보였다.