• Asian-Australasian Journal of Animal Sciences
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• v.24 no.1
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• pp.28-36
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• 2011

To investigate the genetic diversity of six Chinese indigenous pig breeds in Shandong province (Laiwu Black, Dapulian Black, Licha Black, Yantai Black, Yimeng Black and Wulian Black), explain their genetic relationship and assess their integrity and degree of admixture with three Western commercial breeds (Landrace, Yorkshire and Duroc), 303 individuals from these breeds were genotyped for 26 microsatellite markers. In general, high genetic diversity (observed heterozygosity ranging from 0.5495 to 0.7746) and large breed differentiation ($F_{ST}$ = 0.188) were observed. The indigenous pig breeds in Shandong exhibited consistently higher levels of genetic diversity than the three Western breeds. However, compared with the Western breeds, which have an $F_{ST}$ value of 0.252, the indigenous breeds in Shandong have smaller $F_{ST}$ value of 0.145. The analysis of breed relationship indicated that the six indigenous breeds are classified into two groups. One includes four breeds, Licha, Yantai, Yimeng and Wulian, which have experienced large gene introgression of the Western breeds through progressive crossbreeding as well as gene flow among themselves. The other includes Laiwu and Dapulian, which are less influenced by the Western breeds and other indigenous breeds in Shandong in the recent past. The results show that some measures must be taken to effectively protect these indigenous pig breeds in Shandong.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.763-767
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• 2014

Aim: To study the contribution of genetic variation in RAD51 to risk of esophageal squamous cell carcinoma (ESCC). Methods: Three single nucleotide polymorphisms (SNPs) in RAD51 (rs1801320, rs4144242 and rs4417527) were genotyped in 316 ESCC patients and 316 healthy controls in Anyang area of China using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Demographic variables between cases and controls were statistically compared by T test and Chi-square test. Hardy-Weinberg equilibrium was evaluated by the Chi-square test. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to measure any association with ESCC. Haplotype frequencies were estimated by Phase 2.1. Result: The genotype frequencies of rs1801320, rs4144242 and rs4417527 in patients with ESCC demonstrated no significant differences from those in control group (P>0.05). When the haplotypes of these three SNPs were constructed and their relationships with ESCC risk investigated, however, CGG was observed to increase the risk (P=0.020, OR=2.289). Conclusions: There was no association between the three SNPs of RAD51 and ESCC susceptibility in our Chinese population. However, the CGG haplotype might be a risk factor.

• Geomechanics and Engineering
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• v.28 no.6
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• pp.573-584
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• 2022

Water inrush may occur during seaside urban tunnel excavation. Various factors affect the water inrush, and the water inrush mechanism is complex. In this study, nine evaluation indices having potential effects on water inrush were analysed. Specifically, the geographic and geomorphic conditions, unfavourable geology, distance from the tunnel to sea, strength of the surrounding rock, groundwater level, tidal action, cyclical footage, grouting pressure, and grouting reinforced region were analysed. Furthermore, a two-step interval risk assessment method for water inrush management during seaside urban tunnel excavation was developed by a multi-index system and interval risk assessment comprised of an interval analytic hierarchy process, fuzzy comprehensive evaluation, and relative superiority analysis. The novel assessment method was applied to the Haicang Tunnel successfully. A preliminary interval risk assessment method for water inrush was performed based on engineering geological conditions. As a result, the risk level fell into a risk level IV, which represents a section with high risk. Subsequently, a secondary interval risk assessment method was performed based on engineering geological conditions and construction conditions. The risk level of water inrush is reduced to a risk level II. The results agreed with the current tunnel situation, which verified the reliability of this approach.

• Journal of the Optical Society of Korea
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• v.19 no.5
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• pp.467-476
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• 2015

Finger vein recognition, which is a promising biometric method for identity authentication, has attracted significant attention. Considerable research focuses on transmission-type finger vein recognition, but this type of authentication is difficult to implement in mobile consumer devices. Therefore, reflection-type finger vein recognition should be developed. In the reflection-type vein recognition field, the majority of researchers concentrate on palm and palm dorsa patterns, and only a few pay attention to reflection-type finger vein recognition. Thus, this paper presents reflection-type finger vein recognition for biometric application that can be integrated into mobile consumer devices. A database is built to test the proposed algorithm. A novel method of region-of-interest localization for a finger vein image is introduced, and a scheme for effectively extracting finger vein features is proposed. Experiments demonstrate the feasibility of reflection-type finger vein recognition.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.607-613
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• 2019

Objective: Intramuscular fat (IMF) content plays an important role in meat quality. Identification of single nucleotide polymorphisms (SNPs) and genes related to pig IMF, especially using pig populations with high IMF content variation, can help to establish novel molecular breeding tools for optimizing IMF in pork and unveil the mechanisms that underlie fat metabolism. Methods: We collected muscle samples of 453 Chinese Lulai black pigs, measured IMF content by Soxhlet petroleum-ether extraction method, and genotyped genome-wide SNPs using GeneSeek Genomic Profiler Porcine HD BeadChip. Then a genome-wide association study was performed using a linear mixed model implemented in the GEMMA software. Results: A total of 43 SNPs were identified to be significantly associated with IMF content by the cutoff p<0.001. Among these significant SNPs, the greatest number of SNPs (n = 19) were detected on Chr.9, and two linkage disequilibrium blocks were formed among them. Additionally, 17 significant SNPs are mapped to previously reported quantitative trait loci (QTLs) of IMF and confirmed previous QTLs studies. Forty-two annotated genes centering these significant SNPs were obtained from Ensembl database. Overrepresentation test of pathways and gene ontology (GO) terms revealed some enriched reactome pathways and GO terms, which mainly involved regulation of basic material transport, energy metabolic process and signaling pathway. Conclusion: These findings improve our understanding of the genetic architecture of IMF content in pork and facilitate the follow-up study of fine-mapping genes that influence fat deposition in muscle.

• Animal Bioscience
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• v.34 no.7
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• pp.1123-1133
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• 2021

Objective: Shandong indigenous pig breeds are important Chinese pig resources. Their progressive population decline in recent decades has attracted attention towards their conservation. Conservation genetics of these indigenous breeds are essential for developing a conservation and utilization scheme. Methods: A high-density single nucleotide polymorphism (HD-SNP) chip-based comparative analysis of genetic characteristics was performed for seven Shandong indigenous pig breeds in the context of five Western commercial breeds. Results: The results showed that Shandong indigenous pig breeds varied greatly in genetic diversity, effective population size, inbreeding level, and genetic distance with the Western commercial breeds. Specifically, Laiwu and Dapulian displayed low genetic diversity, and had a genetically distant relationship with the Western commercial breeds (average F statistics [F_ST] value of 0.3226 and 0.2666, respectively). Contrastingly, the other five breeds (Yantai, Licha, Yimeng, Wulain, and Heigai) displayed high genetic diversity within breed and had some extent of mixture pattern with the Western commercial breeds, especially Duroc and Landrace (F_ST values from 0.1043 to 0.2536). Furthermore, intensive gene flow was discovered among the seven Shandong indigenous breeds, particularly Wulian, Licha, and Heigai, as indicated by the large cluster formed in the principal component analysis scatterplot and small population differentiation (average of 0.1253) among them. Conclusion: Our study advances the understanding of genetic characteristics of Shandong indigenous breeds and provides essential information for developing an appropriate conservation and utilization scheme for these breeds.

• International Journal of Naval Architecture and Ocean Engineering
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• v.10 no.5
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• pp.651-659
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• 2018

A multi-level overtopping wave energy converter was designed according to the large tidal range and small wave heights in China. It consists of two reservoirs with sloping walls at different levels. The reservoirs share a common outflow duct and a low-head axial turbine. The experimental study was carried out in a laboratory wave-flume to investigate the overtopping performance of the device. The depth-gauges were used to measure the variation of the water level in the reservoirs. The data was processed to derive the time-averaged overtopping discharges. It was found that the lower reservoir can store wave waters at the low water level and break the waves which try to climb up to the upper reservoir. The upper sloping angle and the opening width of the lower reservoir both have significant effects on the overtopping discharges, which can provide more information to the design and optimization of this type of device.

• Journal of Korea Trade
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• v.27 no.3
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• pp.1-20
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• 2023

Purpose - Using trade data from 2008 to 2019, this study analyzes the impact of trade facilitation on China's agricultural exports under the RCEP framework using a gravity model based on the level of trade facilitation in 13 RCEP countries. Design/methodology - This study constructs a complete set of trade facilitation index systems, comprehensively measures the trade facilitation level of RCEP member countries, and uses a gravity model to verify the critical role of trade facilitation level in enhancing the trade volumes of RCEP member countries. Findings - We found that trade facilitation has a significant impact on China's agricultural exports as a whole. The effect of each primary indicator varies in magnitude, with finance and e-commerce (F) having the most significant impact, followed by customs efficiency (C) and infrastructure development (1); the institutional environment has no significant effect. Originality/value - This study analyzes the impact of trade facilitation on China's agricultural exports from the perspective of exports, and uses the latest data to study the degree of the impact of trade facilitation in importing countries. Measures to jointly enhance trade facilitation among member countries under the RCEP framework are proposed.

• Earthquakes and Structures
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• v.25 no.2
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• pp.135-148
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• 2023

The structure-soil-structure interaction (SSSI) effect in adjacent structures may affect the liquefaction-induced damage of shallow foundation structures. The existing studies only analysed the independent effects on the structural dynamic response but ignored the coupling effect of height difference and distance of adjacent structures (F) on liquefied foundations on the dynamic response. Therefore, this paper adopts finite element and finite difference coupled dynamic analysis method to discuss the effect of the F on the seismic response of shallow foundation structures. The results show that the effect of the short structure on the acceleration response of the tall structure can be neglected as F increases when the height difference reaches 2 times the height of the short structure. The beneficial effect of SSSI on short structures is weakened under strong seismic excitations, and the effect of the increase of F on the settlement ratio gradually decreases, which causes a larger rotation hazard. When the distance is smaller than the foundation width, the short structure will exceed the rotation critical value and cause structural damage. When the distance is larger than the foundation width, the rotation angle is within the safe range (0.02 rad).

• Journal of Forest and Environmental Science
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• v.28 no.4
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• pp.242-246
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• 2012

The variation in random amplified polymorphic DNA (RAPD) markers were examined for Xanthoceras sorbifolium Bunge seeds from three plantations (Inner Mongolia [IM], Liaoning [LN], and Shandong [SD] province) in China. Mean genetic variation was measured by polymorphism percentage (42.10%) and expected heterozygosity (He=1.27). Among three populations, Shandong showed the highest values both in polymorphism percentage and heterozygosity (p=57.89; Ho=1.58; and He=1.37). Total genetic diversity value, based on the total loci, was estimated as total genetic diversity of the species (Ht)=0.27 and mean within-population genetic diversity (Hs)=0.16. UPGMA cluster analysis showed the genetic closeness between Inner Mongolia and Liaoning population, but that Shandong seems to be the separate population.