• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.128-134
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• 2008

The kinetics of cell growth and Cyclosporin A (Cyc A) production by Tolypocladium inflatum were studied in shake flasks and bioreactors under controlled and uncontrolled pH conditions. In the case of the shake flask, the production time was extended to 226 h and the maximal antibiotic concentration was 76 mg/l. When scaling up the cultivation process to a bioreactor level, the production time was reduced to only 70h with a significant increase in both the cell growth and the antibiotic production. The maximal dry cell weights in the case of the controlled pH and uncontrolled pH cultures in the bioreactor were 22.4g/l and 14.2g/l, respectively. The corresponding maximal dry cell weight values did not exceed 7.25g/l with the shake flask cultures. The maximal values for Cyc A production were 144.72 and 131.4 mg/l for the controlled and uncontrolled pH cultures, respectively. It is also worth noting that a significant reduction was observed in both the dry cell mass and the antibiotic concentration after the Cyc A production phase, whereas the highest rate of antibiotic degradation was observed in the stirred tank bioreactor with an uncontrolled pH. Morphological characterization of the micromorphological cell growth (mycelial/pellet forms) was also performed during cultivation in the bioreactor.

• Steel and Composite Structures
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• v.28 no.2
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• pp.223-231
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• 2018

Nowadays, there are a great number of various structures that have been retrofitted by using different FRP Composites. Due to this, more researches need to be conducted to know more the characteristics of these structures, not only that but also a comparison among them before and after the retrofitting is needed. In this research, a model steel structure is tested using a bench-scale earthquake simulator on the shake table, using recorded micro tremor data, in order to get the dynamic behaviors. Columns of the model steel structure are then retrofitted by using GFRP composite, and then tested on the Quanser shake table by using the recorded micro tremor data. At this stage, it is needed to evaluate the dynamic behaviors of the retrofitted model steel structure. Various types of methods of OMA, such as EFDD, SSI, etc. are used to take action in the ambient responses. Having a purpose to learn more about the effects of GFRP composite, experimental model analysis of both types (retrofitted and no-retrofitted models) is conducted to evaluate their dynamic behaviors. There is a provision of ambient excitation to the shake table by using recorded micro tremor ambient vibration data on ground level. Furthermore, the Enhanced Frequency Domain Decomposition is used through output-only modal identification. At the end of this study, moderate correlation is obtained between mode shapes, periods and damping ratios. The aim of this research is to show and determine the effects of GFRP Composite implementation on structural responses of the model steel structure, in terms of changing its dynamical behaviors. The frequencies for model steel structure and the retrofitted model steel structure are shown to be 33.916% in average difference. Finally, it is shown that, in order to evaluate the period and rigidity of retrofitted structures, OMA might be used.

• Earthquakes and Structures
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• v.23 no.1
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• pp.23-34
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• 2022

This paper presents experimental studies on reinforced concrete moment resisting frames that have engineered cementitious composite (ECC) in plastic hinge length (PHL) of beam/column members and beam-column joints. A two-story frame structure reduced by a 1:3 scale was further tested through a shake-table (seismic simulator) using multiple levels of simulated earthquake motions. One model conformed to all the ACI-318 requirements for IMRF, whereas the second model used lower-strength concrete in the beam/column members outside PHL. The acceleration time history of the 1994 Northridge earthquake was selected and scaled to multiple levels for shake-table testing. This study reports the observed damage mechanism, lateral strength-displacement capacity curve, and the computed response parameters for each model. The tests verified that nonlinearity remained confined to beam/column ends, i.e., member joint interface. Calculated response modification factors were 11.6 and 9.6 for the code-conforming and concrete strength deficient models. Results show that the RC-ECC frame's performance in design-based and maximum considered earthquakes; without exceeding maximum permissible drift under design-base earthquake motions and not triggering any unstable mode of damage/failure under maximum considered earthquakes. This research also indicates that the introduction of ECC in PHL of the beam/column members' detailing may be relaxed for the IMRF structures.

• Computers and Concrete
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• v.33 no.2
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• pp.195-204
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• 2024

Floor inertial forces are transferred to lateral force resisting systems through a diaphragm action during earthquakes. The diaphragm action requires floor slabs to carry in-plane forces. In precast concrete diaphragms, these forces must be carried across the joints between precast floor units as they represent planes of weakness. Therefore, diaphragm reinforcement with sufficient strength and deformability is necessary to ensure the diaphragm action for the floor inertial force transfer. In a shake table test for a three-story precast concrete structure, an unexpected local failure in the diaphragm flexural reinforcement occurred. This failure caused loss of the diaphragm action but did not trigger collapse of the structure due to a possible alternative path for the floor inertial force transfer. This paper investigates this failure event and its impact on structural seismic responses based on the shake table test and simulation results. The simulations were conducted on a structural model with discrete diaphragm elements. The structural model was also validated from the test results. The investigation indicates that additional floor inertial force will be transferred into the gravity columns after loss of the diaphragm action which can further result in the increase of seismic demands in the gravity column and diaphragms in adjacent floors.

• Journal of Plant Biotechnology
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• v.5 no.3
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• pp.187-191
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• 2003

This paper reported the establishment of mass production system of adventitious roots of Eleutherococcus sessiliflorus through the shake flask and bio-reactor culture. Induction of adventitious roots was started from the explants of germinated somatic embryos on half-strength Murashing and Skoog (MS) solid medium. The frequency of adventitious root formation was better in the explants comprising the basal hypocotyl parts than root explants alone. Among the different auxins tested (NAA, IBA and IAA), frequency of adventitious root induction was highest on medium with 0.5 mg/L NAA, and produced $16.3\pm1.9$ roots per explant. In shake-flask culture, deletion of $NH_4NO_3$ of MS medium was effective for induction of adventitious root compared with both full and half-strength MS media. Fresh weight increase of induced adventitious roots was performed well in medium with 0.5 mg/L IBA. When adventitious roots produced in shake-flask culture were transferred to 10-liter bioreactor, 5.5 times of fresh weight increase was gained after one month of culture. HPLC analysis revealed that the amount of eleutheroside E and E1 was higher in in vitro cultured adventitious roots than the 3 year-old field cultivated root barks of Eleutherococcus sessiliflorus. The content of eltutheroside B was much lower in adventitious roots than that of field cultivated one.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.4
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• pp.97-102
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• 2008

The vibration of a vehicle, which is caused by and transmitted from the engine, has significant effect on the ride comfort and the dynamic characteristics of the engine mount system have direct influence on the vibration and noise of the vehicle. This paper examines the body shake caused by the engine excitation force on engine key on/off of a medium truck by experiment and simulation. The analysis model consists of the engine, a body including the frame, front and rear suspensions and tires. The force element between the body and the suspension is modeled as a combination of a suspension spring and a damper. The engine shake obtained from the experiment was compared with the result of the computer simulation, and by using the verified computer model, parametric study of the body shake on engine key on/off is performed with changing the stiffness of an engine mount rubber, the engine mount angle, and the position of engine mounts.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.10
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• pp.4665-4671
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• 2013

Engine mounting system is the most responsible system for NVH performance of vehicle. The vibration at idle shake, road shake, Key ON/OFF, gear shift tuned by the engine mount position and stiffness. Previously described Engine mounting system theory investigated and summarized in this paper. Decoupling of the Power train rigid mode and Reducing the angle between Torque-Roll-Axis and Elastic-roll-Axis is starting point of optimization. Multi-optimization analysis was performed because of variety simulation case and FE-model. Eventually, Find the best mount location and the stiffness has improved the performance of the vehicle NVH.

• Journal of the Earthquake Engineering Society of Korea
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• v.17 no.1
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• pp.21-31
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• 2013

In this study, dynamic characteristics and seismic capacity of the nuclear power plant piping system are evaluated by model test results using multi-platform shake table. The model is 21.2 m long and consists of straight pipes, elbows, and reducers. The stainless steel pipe diameters are 60.3 mm (2 in.) and 88.9 mm (3 in.) and the system was assembled in accordance with ASME code criteria. The dynamic characteristics such as natural frequency, damping and acceleration responses of the piping system were estimated using the measured acceleration, displacement and strain data. The natural frequencies of the specimen were not changed significantly before and after the testing and the failure and leakage of the piping system was not observed until the final excitation. The damping ratio was estimated in the range of 3.13 ~ 4.98 % and it is found that the allowable stress(345 MPa) according to ASME criteria is 2.5 times larger than the measured maximum stress (138 MPa) of the piping system even under the maximum excitation level of this test.

• Steel and Composite Structures
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• v.21 no.4
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• pp.805-827
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• 2016

A new type of precast CFST column to RC beam braced frame is proposed in this paper. A series of shake table tests were conducted to excite a one-third scale six-story model for investigating the global seismic performance of this type of structure against earthquake actions. Particular emphasis was given to its dynamic property, global seismic responses and failure path. Correspondingly, a numerical model built on the basis of fiber-beam-element model, multi-layer shell model and element-deactivation method was developed to simulate the seismic performance of the prototype structure. Numerical results were compared with the measured values from shake table tests to verify the validity and reliability of the numerical model. The results demonstrated that the proposed novel precast CFST column to RC beam braced frame performs excellently under strong earthquake excitations; the "strong CFST column-weak RC beam" and "strong connection-weak member" anti-seismic design principles can be easily achieved; the maximum deflections of precast CFSTC-RCB braced frame satisfied the deflection limitations proposed in national code; the numerical model can properly simulate the dynamic property and responses of the precast CFSTC-RCB braced frame that are highly concerned in engineering practice.

• Fashion & Textile Research Journal
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• v.3 no.3
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• pp.277-282
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• 2001

The effect of chitosan on antibacterial activities of cotton, wool and polyester fibers was investigated by shake flask method. Chitosan was treated in 0.1%, 1% and 2% $NaBO_3$ solution to reduce the molecular weight in 4 steps, wadding of cotton, wool and polyester were treated in 0.1%, 0.3% and 0.5% of chitosan solution which were dissolved in 2% acetic acid aqueous solution. The antibacterial activities of the fiber wadding treated and untreated by chitosan against Escherichia coli, Proteus vulgaris and Stephylococcus aureus were measured by shake flask method. On the untreated waddings, cotton showed better antibacterial activities than wool, but on the treated ones, wool showed better than cotton. The antibacterial activity of polyester was better than that of cotton or wool which preserved before and after the chitosan treatment against the three kinds of bacteria. When the chitosan treated cotton waddings was retreated in NaOH aqueous solution, their bacterial activities decreased. After laundering, the antibacterial activities of the treated cotton and wool waddings kept good, but that of the treated polyester reduced by almost half.